CNRS - AIX MARSEILLE UNIV : UMR7257

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Silvia SPINELLI

Ingénieur de recherche

AFMB UMR 7257 Case 932
Campus de Luminy
163 Avenue de Luminy
13288 Marseille CEDEX 09
Fax : 04 91 26 67 20

Tel: 04 91 82 55 93
Silvia.Spinelli afmb.univ-mrs.fr
 

Interactions Hôte-Pathogène

 

Curriculum Vitae

  • 1980 Licence en Physique. Universidad Nacional de La Plata, La Plata, Argentina
  • 1985 Doctorat en Physique. Universidad Nacional de La Plata, La Plata, Argentina. Thèse : "Structure tridimensionnelle du fragment Fab d’un anticorps anti-2-phenyloxazomone"
  • Stages post-doctoraux :
    • 1990 - 1993, Laboratoire d’Immunologie Structurale et Laboratoire d’Immunologie Moléculaire, Institut Pasteur, Paris, France
    • 1993 - 1995, AFMB, Marseille
  • Depuis 1995, Ingénieur de Recherches 1 (IR1) CNRS au sein de l’AFMB

Dernières Publications

  1. Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S , Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
    Mol Cell in press
  2. Phase transition and amyloid formation by a viral protein as an additional molecular mechanism of virus-induced cell toxicity. (2018)
    Salladini E, Debarnot C, Delauzun V, Murrali MG, Sutto-Ortiz P, Spinelli S , Pierattelli R, Bignon C, Longhi S.
    BioRxiv
  3. Douzi B, Logger L, Spinelli S , Blangy S, Cambillau C, Cascales E
    J Mol Biol 430 297-309
  4. Zhu J, Arena S, Spinelli S , Liu D, Zhang G, Wei R, Cambillau C, Scaloni A, Wang G, Pelosi P
    Proc Natl Acad Sci U S A 114 E9802-E9810
  5. Desmyter A, Spinelli S , Boutton C, Saunders M, Blachetot C, de Haard H, Denecker G, Van Roy M, Cambillau C, Rommelaere H
    Front Immunol 8 884

PDB Codes

  1. Structure and activity of AbiQ, a lactococcal endoribonuclease belonging to the type-III toxin-antitoxin system. (2013)
    Samson JE, Spinelli S , Cambillau C, Moineau S
    Mol Microbiol 87 756-68
  2. Crystal structure of Apis mellifera OBP14, a C-minus odorant-binding protein, and its complexes with odorant molecules. (2012)
    Spinelli S , Lagarde A, Iovinella I, Legrand P, Tegoni M, Pelosi P, Cambillau C
    Insect Biochem Mol Biol 42 41-50
  3. Crystal structure of Apis mellifera OBP14, a C-minus odorant-binding protein, and its complexes with odorant molecules. (2012)
    Spinelli S , Lagarde A, Iovinella I, Legrand P, Tegoni M, Pelosi P, Cambillau C
    Insect Biochem Mol Biol 42 41-50
  4. Crystal structure of Apis mellifera OBP14, a C-minus odorant-binding protein, and its complexes with odorant molecules. (2012)
    Spinelli S , Lagarde A, Iovinella I, Legrand P, Tegoni M, Pelosi P, Cambillau C
    Insect Biochem Mol Biol 42 41-50
  5. Crystal structure of Apis mellifera OBP14, a C-minus odorant-binding protein, and its complexes with odorant molecules. (2012)
    Spinelli S , Lagarde A, Iovinella I, Legrand P, Tegoni M, Pelosi P, Cambillau C
    Insect Biochem Mol Biol 42 41-50
  6. Crystal structure of Apis mellifera OBP14, a C-minus odorant-binding protein, and its complexes with odorant molecules. (2012)
    Spinelli S , Lagarde A, Iovinella I, Legrand P, Tegoni M, Pelosi P, Cambillau C
    Insect Biochem Mol Biol 42 41-50
  7. Crystal structure of Apis mellifera OBP14, a C-minus odorant-binding protein, and its complexes with odorant molecules. (2012)
    Spinelli S , Lagarde A, Iovinella I, Legrand P, Tegoni M, Pelosi P, Cambillau C
    Insect Biochem Mol Biol 42 41-50
  8. Crystal structure of Apis mellifera OBP14, a C-minus odorant-binding protein, and its complexes with odorant molecules. (2012)
    Spinelli S , Lagarde A, Iovinella I, Legrand P, Tegoni M, Pelosi P, Cambillau C
    Insect Biochem Mol Biol 42 41-50
  9. Structural characterization and oligomerization of the TssL protein, a component shared by the bacterial Type VI and Type IVb secretion systems. (2012)
    Durand E, Zoued A, Spinelli S , Watson PJ, Aschtgen MS, Journet L, Cambillau C, Cascales E
    J Biol Chem 287 14157-68
  10. Structure of the phage TP901-1 1.8 MDa baseplate suggests an alternative host adhesion mechanism. (2012)
    Veesler D, Spinelli S , Mahony J, Lichiere J, Blangy S, Bricogne G, Legrand P, Ortiz-Lombardia M, Campanacci V, van Sinderen D, Cambillau C
    Proc Natl Acad Sci U S A 109 8954-8958
  11. Structure of the phage TP901-1 1.8 MDa baseplate suggests an alternative host adhesion mechanism. (2012)
    Veesler D, Spinelli S , Mahony J, Lichiere J, Blangy S, Bricogne G, Legrand P, Ortiz-Lombardia M, Campanacci V, van Sinderen D, Cambillau C
    Proc Natl Acad Sci U S A 109 8954-8958
  12. Structure of the phage TP901-1 1.8 MDa baseplate suggests an alternative host adhesion mechanism. (2012)
    Veesler D, Spinelli S , Mahony J, Lichiere J, Blangy S, Bricogne G, Legrand P, Ortiz-Lombardia M, Campanacci V, van Sinderen D, Cambillau C
    Proc Natl Acad Sci U S A 109 8954-8958
  13. Structure of the phage TP901-1 1.8 MDa baseplate suggests an alternative host adhesion mechanism. (2012)
    Veesler D, Spinelli S , Mahony J, Lichiere J, Blangy S, Bricogne G, Legrand P, Ortiz-Lombardia M, Campanacci V, van Sinderen D, Cambillau C
    Proc Natl Acad Sci U S A 109 8954-8958
  14. The Crystal Structure of Odorant Binding Protein 7 from Anopheles gambiae Exhibits an Outstanding Adaptability of Its Binding Site. (2011)
    Lagarde A, Spinelli S , Tegoni M, He X, Field L, Zhou JJ, Cambillau C
    J Mol Biol 414 401-12
  15. The Crystal Structure of Odorant Binding Protein 7 from Anopheles gambiae Exhibits an Outstanding Adaptability of Its Binding Site. (2011)
    Lagarde A, Spinelli S , Tegoni M, He X, Field L, Zhou JJ, Cambillau C
    J Mol Biol 414 401-12
  16. The Crystal Structure of Odorant Binding Protein 7 from Anopheles gambiae Exhibits an Outstanding Adaptability of Its Binding Site. (2011)
    Lagarde A, Spinelli S , Tegoni M, He X, Field L, Zhou JJ, Cambillau C
    J Mol Biol 414 401-12
  17. Crystal structure of Bacillus subtilis SPP1 phage gp22 shares fold similarity with a domain of lactococcal phage p2 RBP. (2010)
    Veesler D, Blangy S, Spinelli S , Tavares P, Campanacci V, Cambillau C
    Protein Sci 19 1439-1443
  18. Bebeacua C, Bron P, Lai L, Vegge CS, Brondsted L, Spinelli S , Campanacci V, Veesler D, van Heel M, Cambillau C
    J Biol Chem 285 39079-86
  19. Siponen M, Sciara G, Villion M, Spinelli S , Lichiere J, Cambillau C, Moineau S, Campanacci V
    J Bacteriol 191 728-734
  20. Pesenti ME, Spinelli S , Bezirard V, Briand L, Pernollet JC, Campanacci V, Tegoni M, Cambillau C
    J Mol Biol 390(5) 981-90
  21. Pesenti ME, Spinelli S , Bezirard V, Briand L, Pernollet JC, Campanacci V, Tegoni M, Cambillau C
    J Mol Biol 390(5) 981-90
  22. Queen bee pheromone binding protein ph induced domain-swapping favors pheromone release. (2009)
    Pesenti ME, Spinelli S , Bezirard V, Briand L, Pernollet JC, Campanacci V, Tegoni M, Cambillau C
    J Mol Biol 390(5) 981-90
  23. Queen bee pheromone binding protein ph induced domain-swapping favors pheromone release. (2009)
    Pesenti ME, Spinelli S , Bezirard V, Briand L, Pernollet JC, Campanacci V, Tegoni M, Cambillau C
    J Mol Biol 390(5) 981-90
  24. Pesenti ME, Spinelli S , Bezirard V, Briand L, Pernollet JC, Campanacci V, Tegoni M, Cambillau C
    J Mol Biol 390(5) 981-90
  25. Queen bee pheromone binding protein ph induced domain-swapping favors pheromone release. (2009)
    Pesenti ME, Spinelli S , Bezirard V, Briand L, Pernollet JC, Campanacci V, Tegoni M, Cambillau C
    J Mol Biol 390(5) 981-90
  26. Pesenti ME, Spinelli S , Bezirard V, Briand L, Pernollet JC, Campanacci V, Tegoni M, Cambillau C
    J Mol Biol 390(5) 981-90
  27. Pesenti ME, Spinelli S , Bezirard V, Briand L, Pernollet JC, Campanacci V, Tegoni M, Cambillau C
    J Mol Biol 390(5) 981-90
  28. Pesenti ME, Spinelli S , Bezirard V, Briand L, Pernollet JC, Campanacci V, Tegoni M, Cambillau C
    J Mol Biol 390(5) 981-90
  29. Queen bee pheromone binding protein ph induced domain-swapping favors pheromone release. (2009)
    Pesenti ME, Spinelli S , Bezirard V, Briand L, Pernollet JC, Campanacci V, Tegoni M, Cambillau C
    J Mol Biol 390(5) 981-90
  30. Crystal structure of a chimeric receptor binding protein constructed from two lactococcal phages. (2009)
    Siponen M, Spinelli S , Blangy S, Moineau S, Cambillau C, Campanacci V
    J Bacteriol 191 3220-5
  31. Crystal structure of a chimeric receptor binding protein constructed from two lactococcal phages. (2009)
    Siponen M, Spinelli S , Blangy S, Moineau S, Cambillau C, Campanacci V
    J Bacteriol 191 3220-5
  32. Crystal structure and function of a DARPin neutralizing inhibitor of lactococcal phage TP901-1: Comparison of DARPin and camelid VHH binding mode. (2009)
    Veesler D, Dreier B, Blangy S, Lichiere J, Tremblay D, Moineau S, Spinelli S , Tegoni M, Pluckthun A, Campanacci V, Cambillau C
    J Biol Chem 284 30718-30726
  33. The crystal structure of ORF14 from Sulfolobus islandicus filamentous virus. (2009)
    Goulet A, Spinelli S , Blangy S, van Tilbeurgh H, Leulliot N, Basta T, Prangishvili D, Cambillau C, Campanacci V
    Proteins 76 1020-2
  34. Structure of Human Pancreatic Lipase-Related Protein 2 with the Lid in an Open Conformation. (2008)
    Eydoux C, Spinelli S , Davis TL, Walker JR, Seitova A, Dhe-Paganon S, De Caro A, Cambillau C, Carriere F
    Biochemistry 47(36) 9553-64
  35. Structure of Human Pancreatic Lipase-Related Protein 2 with the Lid in an Open Conformation. (2008)
    Eydoux C, Spinelli S , Davis TL, Walker JR, Seitova A, Dhe-Paganon S, De Caro A, Cambillau C, Carriere F
    Biochemistry 47(36) 9553-64
  36. Pesenti ME, Spinelli S , Bezirard V, Briand L, Pernollet JC, Tegoni M, Cambillau C
    J Mol Biol 380 158-169
  37. Pesenti ME, Spinelli S , Bezirard V, Briand L, Pernollet JC, Tegoni M, Cambillau C
    J Mol Biol 380 158-169
  38. Pesenti ME, Spinelli S , Bezirard V, Briand L, Pernollet JC, Tegoni M, Cambillau C
    J Mol Biol 380 158-169
  39. Structural Basis of the Honey Bee PBP Pheromone and pH-induced Conformational Change. (2008)
    Pesenti ME, Spinelli S , Bezirard V, Briand L, Pernollet JC, Tegoni M, Cambillau C
    J Mol Biol 380 158-169
  40. Structural Basis of the Honey Bee PBP Pheromone and pH-induced Conformational Change. (2008)
    Pesenti ME, Spinelli S , Bezirard V, Briand L, Pernollet JC, Tegoni M, Cambillau C
    J Mol Biol 380 158-169
  41. Receptor-Binding Protein of Lactococcus lactis Phages: Identification and Characterization of the Saccharide Receptor-Binding Site. (2006)
    Tremblay DM, Tegoni M, Spinelli S , Campanacci V, Blangy S, Huyghe C, Desmyter A, Labrie S, Moineau S, Cambillau C
    J Bacteriol 188 2400-10
  42. Lactococcal bacteriophage p2 receptor-binding protein structure suggests a common ancestor gene with bacterial and mammalian viruses. (2006)
    Spinelli S , Desmyter A, Verrips CT, de Haard HJ, Moineau S, Cambillau C
    Nat Struct Mol Biol 13 85-9
  43. Lactococcal bacteriophage p2 receptor-binding protein structure suggests a common ancestor gene with bacterial and mammalian viruses. (2006)
    Spinelli S , Desmyter A, Verrips CT, de Haard HJ, Moineau S, Cambillau C
    Nat Struct Mol Biol 13 85-9
  44. Ricagno S, Campanacci V, Blangy S, Spinelli S , Tremblay D, Moineau S, Tegoni M, Cambillau C
    J Virol 80 9331-5
  45. Crystal structure of the temperature-sensitive and allosteric-defective chaperonin GroELE461K. (2006)
    Cabo-Bilbao A, Spinelli S , Sot B, Agirre J, Mechaly AE, Muga A, Guerin DM
    J Struct Biol 155 482-92
  46. Isolation of llama antibody fragments for prevention of dandruff by phage display in shampoo. (2005)
    Dolk E, van der Vaart M, Lutje Hulsik D, Vriend G, de Haard H, Spinelli S , Cambillau C, Frenken L, Verrips T
    Appl Environ Microbiol 71 442-50
  47. A mutation designed to alter crystal packing permits structural analysis of a tight-binding fluorescein-scFv complex. (2005)
    Honegger A, Spinelli S , Cambillau C, Pluckthun A
    Protein Sci 14 2537-49
  48. Crystal structures of bovine odorant-binding protein in complex with odorant molecules. (2004)
    Vincent F, Ramoni R, Spinelli S , Grolli S, Tegoni M, Cambillau C
    Eur J Biochem 271 3832-3842
  49. Crystal structures of bovine odorant-binding protein in complex with odorant molecules. (2004)
    Vincent F, Ramoni R, Spinelli S , Grolli S, Tegoni M, Cambillau C
    Eur J Biochem 271 3832-3842
  50. Crystal structures of bovine odorant-binding protein in complex with odorant molecules. (2004)
    Vincent F, Ramoni R, Spinelli S , Grolli S, Tegoni M, Cambillau C
    Eur J Biochem 271 3832-3842
  51. Crystal structures of bovine odorant-binding protein in complex with odorant molecules. (2004)
    Vincent F, Ramoni R, Spinelli S , Grolli S, Tegoni M, Cambillau C
    Eur J Biochem 271 3832-3842
  52. The Escherichia coli YadB Gene Product Reveals a Novel Aminoacyl-tRNA Synthetase Like Activity (2004)
    Campanacci V, Dubois DY, Becker HD, Kern D, Spinelli S , Valencia C, Pagot F, Salomoni A, Brisel S, Vincentelli R, Bignon C, Lapointe J, Giege R, Cambillau C
    J Mol Biol 337 273-283
  53. Crystal Structure of E.coli Alcohol Dehydrogenase YqhD: Evidence of a Covalently Modified NADP Coenzyme. (2004)
    Sulzenbacher G, Alvarez K, Van Den Heuvel RH, Versluis C, Spinelli S , Campanacci V, Valencia C, Cambillau C, Eklund H, Tegoni M
    J Mol Biol 342 489-502
  54. Directed in vitro evolution and crystallographic analysis of a peptide binding scFv antibody with low picomolar affinity. (2004)
    Zahnd C, Spinelli S , Luginbuhl B, Amstutz P, Cambillau C, Pluckthun A
    J Biol Chem 279 18870-18877
  55. The crystal structure of the Escherichia coli lipocalin Blc suggests a possible role in phospholipid binding. (2004)
    Campanacci V, Nurizzo D, Spinelli S , Valencia C, Tegoni M, Cambillau C
    FEBS Lett 562 183-8
  56. Domain swapping of a llama VHH domain builds a crystal-wide beta-sheet structure. (2004)
    Spinelli S , Desmyter A, Frenken L, Verrips T, Tegoni M, Cambillau C
    FEBS Lett 564 35-40
  57. Lartigue A, Gruez A, Spinelli S , Riviere S, Brossut R, Tegoni M, Cambillau C
    J Biol Chem 278 30213-30218
  58. Boar salivary lipocalin. Three-dimensional X-ray structure and androsterol/androstenone docking simulations. (2002)
    Spinelli S , Vincent F, Pelosi P, Tegoni M, Cambillau C.
    Eur J Biochem 269 2449-56
  59. Crystal structure of the open form of dog gastric lipase in complex with a phosphonate (2002)
    Roussel A, Miled N, Berti-Dupuis L, Riviere M, Spinelli S , Berna P, Gruber V, Verger R, Cambillau C
    J Biol Chem 277 2266-2274
  60. Three camelid VHH domains in complex with porcine pancreatic a-amylase: Inhibition and versatility of binding topology. (2002)
    Desmyter A, Spinelli S , Payan F, Lauwereys M, Wyns L, Muyldermans S, Cambillau C
    J Biol Chem 277 23645-23650
  61. Three camelid VHH domains in complex with porcine pancreatic a-amylase: Inhibition and versatility of binding topology. (2002)
    Desmyter A, Spinelli S , Payan F, Lauwereys M, Wyns L, Muyldermans S, Cambillau C
    J Biol Chem 277 23645-23650
  62. Three camelid VHH domains in complex with porcine pancreatic a-amylase: Inhibition and versatility of binding topology. (2002)
    Desmyter A, Spinelli S , Payan F, Lauwereys M, Wyns L, Muyldermans S, Cambillau C
    J Biol Chem 277 23645-23650
  63. Crystal structure of Aphrodisin, a Sex Pheromone from Female Hamster (2001)
    Vincent F, Loebel D, Brown K, Spinelli S , Grote P, Breer H, Cambillau C and Tegoni M
    J Mol Biol 305 459-469
  64. The insect attractant, 1-octen-3-ol is the natural ligand of bovine odorant binding protein (2001)
    Ramoni R, Vincent F, Grolli S, Conti V, Malosse C, Boyer FD,Nagnan-Le Meillour P, Spinelli S , Cambillau C, Tegoni M
    J Biol Chem 276 7150-7155
  65. The insect attractant, 1-octen-3-ol is the natural ligand of bovine odorant binding protein (2001)
    Ramoni R, Vincent F, Grolli S, Conti V, Malosse C, Boyer FD,Nagnan-Le Meillour P, Spinelli S , Cambillau C, Tegoni M
    J Biol Chem 276 7150-7155
  66. The importance of framework residues h6, h7 and h10 in antibody heavy chains: experimental evidence for a new structural subclassification of antibody v(h) domains. (2001)
    Jung S, Spinelli S , Schimmele B, Honegger A, Pugliese L, Cambillau C, Pluckthun A
    J Mol Biol 309 701-716
  67. Selection, characterization and x-ray structure of anti-ampicillin single-chain fv fragments from phage-displayed murine antibody libraries. (2001)
    Burmester J, Spinelli S , Pugliese L, Krebber A, Honegger A, Jung S, Schimmele B, Cambillau C, Pluckthun A
    J Mol Biol 309 681-695
  68. Selection, characterization and x-ray structure of anti-ampicillin single-chain fv fragments from phage-displayed murine antibody libraries. (2001)
    Burmester J, Spinelli S , Pugliese L, Krebber A, Honegger A, Jung S, Schimmele B, Cambillau C, Pluckthun A
    J Mol Biol 309 681-695
  69. The insect attractant, 1-octen-3-ol is the natural ligand of bovine odorant binding protein (2001)
    Ramoni R, Vincent F, Grolli S, Conti V, Malosse C, Boyer FD,Nagnan-Le Meillour P, Spinelli S , Cambillau C, Tegoni M
    J Biol Chem 276 7150-7155
  70. Selection, characterization and x-ray structure of anti-ampicillin single-chain fv fragments from phage-displayed murine antibody libraries. (2001)
    Burmester J, Spinelli S , Pugliese L, Krebber A, Honegger A, Jung S, Schimmele B, Cambillau C, Pluckthun A
    J Mol Biol 309 681-695
  71. Spinelli S , Tegoni M,Frenken L,van Vliet C, Cambillau C
    J Mol Biol 311 123-129
  72. Lateral Recognition of a Dye Hapten by a Llama VHH Domain (2001)
    Spinelli S , Tegoni M,Frenken L,van Vliet C, Cambillau C
    J Mol Biol 311 123-129
  73. Recombinant chemosensory protein (CSP2) from the moth Mamestra brassicae: crystallization and preliminary crystallographic study. (2001)
    Campanacci V, Spinelli S , Lartigue A, Lewandowski C, Brown K, Tegoni M, Cambillau C
    Acta Crystallogr D Biol Crystallogr 57 137-139
  74. Complexes of Porcine Odorant Binding Protein with Odorant Molecules Belonging to Different Chemical Classes (2000)
    Vincent F, Spinelli S , Ramoni R, Grolli S, Pelosi P, Cambillau C, Tegoni M
    J Mol Biol 300 127-139
  75. Complexes of Porcine Odorant Binding Protein with Odorant Molecules Belonging to Different Chemical Classes (2000)
    Vincent F, Spinelli S , Ramoni R, Grolli S, Pelosi P, Cambillau C, Tegoni M
    J Mol Biol 300 127-139
  76. Complexes of Porcine Odorant Binding Protein with Odorant Molecules Belonging to Different Chemical Classes (2000)
    Vincent F, Spinelli S , Ramoni R, Grolli S, Pelosi P, Cambillau C, Tegoni M
    J Mol Biol 300 127-139
  77. Complexes of Porcine Odorant Binding Protein with Odorant Molecules Belonging to Different Chemical Classes (2000)
    Vincent F, Spinelli S , Ramoni R, Grolli S, Pelosi P, Cambillau C, Tegoni M
    J Mol Biol 300 127-139
  78. Complexes of Porcine Odorant Binding Protein with Odorant Molecules Belonging to Different Chemical Classes (2000)
    Vincent F, Spinelli S , Ramoni R, Grolli S, Pelosi P, Cambillau C, Tegoni M
    J Mol Biol 300 127-139
  79. Complexes of Porcine Odorant Binding Protein with Odorant Molecules Belonging to Different Chemical Classes (2000)
    Vincent F, Spinelli S , Ramoni R, Grolli S, Pelosi P, Cambillau C, Tegoni M
    J Mol Biol 300 127-139
  80. Complexes of Porcine Odorant Binding Protein with Odorant Molecules Belonging to Different Chemical Classes (2000)
    Vincent F, Spinelli S , Ramoni R, Grolli S, Pelosi P, Cambillau C, Tegoni M
    J Mol Biol 300 127-139
  81. Crystal structure of a cohesin domain from Clostridium cellulolyticum : implications for the dockerin recognition (2000)
    Spinelli S , Fierobe HP, Belaich A, Belaich JP, Henrissat B, Cambillau C
    J Mol Biol 304 189-200
  82. Complexes of Porcine Odorant Binding Protein with Odorant Molecules Belonging to Different Chemical Classes (2000)
    Vincent F, Spinelli S , Ramoni R, Grolli S, Pelosi P, Cambillau C, Tegoni M
    J Mol Biol 300 127-139
  83. Camelid heavy-chain variable domains provide efficient combining sites to haptens. (2000)
    Spinelli S , Frenken LG, Hermans P, Verrips T, Brown K, Tegoni M, Cambillau C
    Biochemistry 39 1217-1222

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