CNRS - AIX MARSEILLE UNIV: UMR7257

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Structural Biology

Electron microscopy

Head Silvia SPINELLI

The electron microscopy service offers a complementary aproach to x-ray crystallography for the structural characterization of biological systems and large macromolecular assemblies. The service allow users to perform the different steps for the preparation of biological samples by negative staining to enable high contrast imaging form naturally low-contrast objects, imaging acquisition on the embedded camera and image analysis. This approach can be used to characterize the structural homogeneity of a protein sample, generate a low resolution 3D structure or identify large conformational changes in proteins or complexes upon addition of ligands.

Accessibility

Academics and industrials

How to access ?

Specific equipment

1 FEI Tecnai G2 spirit 120 kV equipped with a low dose module and a 2k x 2k CCD camera

Cost

To be defined from the service requested

Publications associated with this facility

  • Bebeacua C, Tremblay D, Farenc C, Chapot-Chartier MP, Sadovskaya I, van Heel M, Veesler D, Moineau S, Cambillau C (2013) Structure, adsorption to host, and infection mechanism of virulent lactococcal phage p2. J Virol 87:12302-12.
  • Desmyter A, Farenc C, Mahony J, Spinelli S, Bebeacua C, Blangy S, Veesler D, van Sinderen D, Cambillau C (2013) Viral infection modulation and neutralization by camelid nanobodies. Proc Natl Acad Sci U S A 110:E1371-9.
  • Sciara G, Bebeacua C, Bron P, Tremblay D, Ortiz-Lombardia M, Lichière J, van Heel M, Campanacci V, Moineau S, Cambillau C. (2010) Structure of lactococcal phage p2 baseplate and its mechanism of activation. Proc Natl Acad Sci USA 10:6852-7.
  • Bignon C, Li C, Lichière J, Canard B, Coutard B (2013) Improving the soluble expression of recombinant proteins by randomly shuffling 5’ and 3’ coding-sequence ends. Acta Crystallogr D 69, 2580-2.
  • Le Breton M, Meyniel-Schicklin L, Deloire A, Coutard B, Canard B, de Lamballerie X, Andre P, Rabourdin-Combe C, Lotteau V, Davoust N (2011) Flavivirus NS3 and NS5 proteins interaction network: a high-throughput yeast two-hybrid screen. BMC Microbiol 11, 234.
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