Christophe Bignon
Research engineer
Research group: Structural Disorder and Molecular Recognition
Publications
2023
Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies
Juliet F Nilsson, Hakima Baroudi, Frank Gondelaud, Giulia Pesce, Christophe Bignon, Denis Ptchelkine, Joseph Chamieh, Hervé Cottet, Andrey V Kajava, Sonia Longhi
International Journal of Molecular Sciences 24:399 (2023)10.3390/ijms24010399
2022
Split-GFP Reassembly Assay: Strengths and Caveats from a Multiparametric Analysis
Christophe Bignon, Antoine Gruet, Sonia Longhi
International Journal of Molecular Sciences 23:13167 (2022)10.3390/ijms232113167
Experimental Evidence of Intrinsic Disorder and Amyloid Formation by the Henipavirus W Proteins
Giulia Pesce, Frank Gondelaud, Denis Ptchelkine, Juliet F Nilsson, Christophe Bignon, Jérémy Cartalas, Patrick Fourquet, Sonia Longhi
International Journal of Molecular Sciences 23:923 (2022)10.3390/ijms23020923
Functional benefit of structural disorder for the replication of measles, Nipah and Hendra viruses
Frank Gondelaud, Giulia Pesce, Juliet F. Nilsson, Christophe Bignon, Denis Ptchelkine, Denis Gerlier, Cyrille Mathieu, Sonia Longhi
Essays in Biochemistry 66:915-934 (2022)10.1042/EBC20220045
2021
Identification of a Region in the Common Amino-terminal Domain of Hendra Virus P, V, and W Proteins Responsible for Phase Transition and Amyloid Formation
Edoardo Salladini, Frank Gondelaud, Juliet Nilsson, Giulia Pesce, Christophe Bignon, Maria Grazia Murrali, Roxane Fabre, Roberta Pierattelli, Andrey Kajava, Branka Horvat, Denis Gerlier, Cyrille Mathieu, Sonia Longhi
Biomolecules 11:1324 (2021)10.3390/biom11091324
Structural and Functional Characterization of the ABA-Water Deficit Stress Domain from Wheat and Barley: An Intrinsically Disordered Domain behind the Versatile Functions of the Plant Abscissic Acid, Stress and Ripening Protein Family
Ines Yacoubi, Karama Hamdi, Patrick Fourquet, Christophe Bignon, Sonia Longhi
International Journal of Molecular Sciences 22:2314 (2021)10.3390/ijms22052314
Structural and dynamics analysis of intrinsically disordered proteins by high-speed atomic force microscopy
Noriyuki Kodera, Daisuke Noshiro, Sujit K Dora, Tetsuya Mori, Johnny Habchi, David Blocquel, Antoine Gruet, Marion Dosnon, Edoardo Salladini, Christophe Bignon, Yuko Fujioka, Takashi Oda, Nobuo N Noda, Mamoru Sato, Marina Lotti, Mineyuki Mizuguchi, Sonia Longhi, Toshio Ando
Nature Nanotechnology 16:181-189 (2021)10.1038/s41565-020-00798-9
2019
Binding induced folding: Lessons from the kinetics of interaction between NTAIL and XD
Angelo Toto, Francesca Troilo, Lorenzo Visconti, Francesca Malagrinò, Christophe Bignon, Sonia Longhi, Stefano Gianni
Archives of Biochemistry and Biophysics 671:255-261 (2019)10.1016/j.abb.2019.07.011
An arsenal of methods for the experimental characterization of intrinsically disordered proteins – How to choose and combine them?
Antoine Schramm, Christophe Bignon, Stefania Brocca, Rita Grandori, Carlo Santambrogio, Sonia Longhi
Archives of Biochemistry and Biophysics 108055 (2019)10.1016/j.abb.2019.07.020
Understanding Intramolecular Crosstalk in an Intrinsically Disordered Protein
Francesca Troilo, Daniela Bonetti, Christophe Bignon, Sonia Longhi, Stefano Gianni
ACS Chemical Biology 14:337-341 (2019)10.1021/acschembio.8b01055
Modulation of Measles Virus NTAIL Interactions through Fuzziness and Sequence Features of Disordered Binding Sites
Christophe Bignon, Francesca Troilo, Stefano Gianni, Sonia Longhi
Biomolecules 9:8 (2019)10.3390/biom9010008
2018
InSiDDe: A Server for Designing Artificial Disordered Proteins
Antoine Schramm, Philippe Lieutaud, Stefano Gianni, Sonia Longhi, Christophe Bignon
International Journal of Molecular Sciences 19:e91 (2018)10.3390/ijms19010091
2017
Partner-Mediated Polymorphism of an Intrinsically Disordered Protein
Christophe Bignon, Francesca Troilo, Stefano Gianni, Sonia Longhi
Journal of Molecular Biology (2017)10.1016/j.jmb.2017.11.012
2016
Modulation of Re-initiation of Measles Virus Transcription at Intergenic Regions by PXD to NTAIL Binding Strength
Louis-Marie Bloyet, Joanna Brunel, Marion Dosnon, Véronique Hamon, Jenny Erales, Antoine Gruet, Carine Lazert, Christophe Bignon, Philippe Roche, Sonia Longhi, Denis Gerlier
PLoS Pathogens 12:e1006058 (2016)10.1371/journal.ppat.1006058
Modulation of Re-initiation of Measles Virus Transcription at Intergenic Regions by PXD to NTAIL Binding Strength
Louis-Marie Bloyet, Joanna Brunel, Marion Dosnon, Véronique Hamon, Jenny Erales, Antoine Gruet, Carine Lazert, Christophe Bignon, Philippe Roche, Sonia Longhi, Denis Gerlier
PLoS Pathogens 12:e1006058 (2016)10.1371/journal.ppat.1006058
Fuzzy regions in an intrinsically disordered protein impair protein-protein interactions
Antoine Gruet, Marion Dosnon, David Blocquel, Joanna Brunel, Denis Gerlier, Rahul K. Das, Daniela Bonetti, Stefano Gianni, Monika Fuxreiter, Sonia Longhi, Christophe Bignon
FEBS Journal 283:576-594 (2016)10.1111/febs.13631
2015
Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia pastoris
Mireille Haon, Sacha Grisel, David Navarro, Antoine Gruet, Jean-Guy Berrin, Christophe Bignon
Frontiers in Microbiology 6 (2015)10.3389/fmicb.2015.01002
2013
Dissecting Partner Recognition by an Intrinsically Disordered Protein Using Descriptive Random Mutagenesis
Antoine Gruet, Marion Dosnon, Andrea Vassena, Vincent Lombard, Denis Gerlier, Christophe Bignon, Sonia Longhi
Journal of Molecular Biology 425:3495-3509 (2013)10.1016/j.jmb.2013.06.025
2012
Single pH buffer refolding screen for protein from inclusion bodies
Bruno Coutard, Etienne Danchin, Rachid Oubelaid, Bruno Canard, Christophe Bignon
Protein Expression and Purification 82:352-359 (2012)10.1016/j.pep.2012.01.014
2009
Interaction between the SifA Virulence Factor and Its Host Target SKIP Is Essential for Salmonella Pathogenesis
Lautaro Diacovich, Audrey Dumont, Daniel Lafitte, Elodie Soprano, Aude-Agnès Guilhon, Christophe Bignon, Jean-Pierre Gorvel, Yves Bourne, Stéphane Méresse
Journal of Biological Chemistry 284:33151-33160 (2009)10.1074/jbc.m109.034975
Interaction between the sifa virulence factor and its host target skip is essential for salmonella pathogenesis.
Lautaro Diacovich, Audrey Dumont, Daniel Lafitte, Elodie Soprano, Aude-Agnes Guilhon, Christophe Bignon, Jean-Pierre Gorvel, Yves Bourne, Stephane Meresse
Journal of Biological Chemistry epub ahead of print (2009)10.1074/jbc.M109.034975
2007
Expression in Escherichia coli, refolding and crystallization of Aspergillus niger feruloyl esterase A using a serial factorial approach
Isabelle Benoit, Bruno Coutard, Rachid Oubelaid, Marcel M. Asther, Christophe Bignon
Protein Expression and Purification 55:166-174 (2007)10.1016/j.pep.2007.04.001
2006
Respective importance of protein folding and glycosylation in the thermal stability of recombinant feruloyl esterase A
Isabelle Benoit, Michèle Asther, Gerlind Sulzenbacher, Eric Record, Laurence Marmuse, Goetz Parsiegla, Isabelle Gimbert, Marcel M. Asther, Christophe Bignon
FEBS Letters 580:5815-5821 (2006)10.1016/j.febslet.2006.09.039
2004
The Three-dimensional Structure of Invertase (β-Fructosidase) from Thermotoga maritima Reveals a Bimodular Arrangement and an Evolutionary Relationship between Retaining and Inverting Glycosidases
Francois Alberto, Christophe Bignon, Gerlind Sulzenbacher, Bernard Henrissat, Mirjam Czjzek
Journal of Biological Chemistry 279:18903-18910 (2004)10.1074/jbc.M313911200
Crystal Structure of Thermotoga maritima ␣-L-Fucosidase INSIGHTS INTO THE CATALYTIC MECHANISM AND THE MOLECULAR BASIS FOR FUCOSIDOSIS
Gerlind Sulzenbacher, Christophe Bignon, Takeshi Nishimura, Chris A Tarling, Stephen G Withers, Bernard Henrissat, Yves Bourne
Journal of Biological Chemistry (2004)10.1074/jbc.M313783200
The Escherichia coli YadB Gene Product Reveals a Novel Aminoacyl-tRNA Synthetase Like Activity
Valérie Campanacci, Daniel Y. Dubois, Hubert Becker, Daniel Kern, Silvia Spinelli, Christel Valencia, Fabienne Pagot, Aurelia Salomoni, Sacha Grisel, Renaud Vincentelli, Christophe Bignon, Jacques Lapointe, Richard Giege, Christian Cambillau
Journal of Molecular Biology 337:273-283 (2004)10.1016/j.jmb.2004.01.027
2003
Identification of the Catalytic Nucleophile of the Family 29 α-L-Fucosidase from Thermotoga maritima through Trapping of a Covalent Glycosyl-Enzyme Intermediate and Mutagenesis
Chris A Tarling, Shouming He, Gerlind Sulzenbacher, Christophe Bignon, Yves Bourne, Bernard Henrissat, Stephen G Withers
Journal of Biological Chemistry (2003)10.1074/jbc.M306610200
Medium-Scale Structural Genomics: Strategies for Protein Expression and Crystallization
Renaud Vincentelli, Christophe Bignon, Arnaud Gruez, Stéphane Canaan, Gerlind Sulzenbacher, Mariella Tegoni, Rie Campanacci, Christian Cambillau
Accounts of Chemical Research (2003)10.1021/ar010130s
2002
A medium-throughput crystallization approach
Gerlind Sulzenbacher, Arnaud Gruez, Véronique Roig-Zamboni, Silvia Spinelli, Christel Valencia, Fabienne Pagot, Renaud Vincentelli, Christophe Bignon, Aurelia Salomoni, Sacha Grisel, Damien Maurin, Céline Huyghe, Kent Johansson, Alice Grassick, Alain Roussel, Yves Bourne, Sophie Perrier, Linda Miallau, Phillippe Cantau, Eric Blanc, Michel Genevois, Alain Grossi, André Zenatti, Valérie Campanacci, Christian Cambillau
Acta crystallographica Section D : Structural biology [1993-...] 58:2109-2115 (2002)10.1107/s0907444902013938
1998
Cloning, preparation and characterization of biologically active recombinant caprine placental lactogen
E. Sakal, Christophe Bignon, N. Chapnik-Cohen, Nathalie Daniel-Carlier, Jacqueline Paly, L. Belair, Jean Djiane, A. Gertler
Journal of Endocrinology 159:509-518 (1998)10.1677/joe.0.1590509
