CNRS - AIX MARSEILLE UNIV: UMR7257

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Host-pathogen interactions

Head Alain ROUSSEL

Latest Publications

  1. How disordered is my protein and what is its disorder for? A guide through the dark side of the protein universe. (2016) Lieutaud P, Ferron F, Uversky AV, Kurgan L, Uversky VN and Longhi S.. Intrinsically Disordered Proteins 4:1 e1259708 PMID:0
  2. Structure and specificity of the Type VI secretion system ClpV-TssC interaction in enteroaggregative Escherichia coli. (2016) Douzi B, Brunet YR, Spinelli S, Lensi V, Legrand P, Blangy S, Kumar A, Journet L, Cascales E, Cambillau C. Sci Rep 6 34405 PMID:27698444
  3. Structure-function analysis of the TssL cytoplasmic domain reveals a new interaction between the Type VI secretion baseplate and membrane complexes. (2016) Zoued A, Cassaro CJ, Durand E, Douzi B, Espana AP, Cambillau C, Journet L, Cascales E. J Mol Biol 428 4413-4423 PMID:27600409
  4. Characterization of prophages containing "evolved" Dit/Tal modules in the genome of Lactobacillus casei BL23. (2016) Dieterle ME, Fina Martin J, Duran R, Nemirovsky SI, Sanchez Rivas C, Bowman C, Russell D, Hatfull GF, Cambillau C, Piuri M. Appl Microbiol Biotechnol 100 9201-9215 PMID:27448399
  5. Structure of the host-recognition device of Staphylococcus aureus phage varphi11. (2016) Koc C, Xia G, Kuhner P, Spinelli S, Roussel A, Cambillau C, Stehle T. Sci Rep 6 27581 PMID:27282779
  6. The baseplate of Lactobacillus delbrueckii bacteriophage Ld17 harbours a glycerophosphodiesterase. (2016) Cornelissen A, Sadovskaya I, Vinogradov E, Blangy S, Spinelli S, Casey E, Mahony J, Noben JP, Dal Bello F, Cambillau C, van Sinderen D. J Biol Chem 291 16816-27 PMID:27268053
...All publications

Our group aims at understanding and analyzing the interactions of pathogens with their host, leading to its infection and its possible destruction. Our targets of interest are diverse, they gather i) bacteriophages infecting, among others, the lactic acid bacterium Lactococcus lactis used in the dairy industry, ii) bacterial secretion systems of type 6 and type 9, iii) the immune innate response of Drosophila after microbial or viral infection.

Understanding of host-pathogen interactions is at the heart of fighting pathogens involved in infectious diseases (bacteria, viruses). During the last ten years, we studied the structure and adhesion mechanism of bacteriophages (viruses that infect bacteria) infecting the bacterium used in the milk industry Lactococcus lactis. We have determined the structures of several phage virions by electron microscopy, and of the anti-receptor or the baseplate (1-2 MDa), eventually complexed to the receptor, by X-ray diffraction. We found that some phages, but not all, require an activation step by the calcium ion. More recently, we have addressed the study of type VI (T6SS) and Type IX (T9SS) secretion systems. We determined the structure and interactions of some of the 13 components of the T6SS, and we pursue the study of the system, including the trans-membrane domains. We wish to reconstruct the whole system by a "hybrid" approach, including electron microscopy and diffraction. The same approach is applied to T9SS. Finally, we have chosen the Drosophila model organism to study the innate immune response of the host. Genetic analysis has delineated two major regulatory pathways, the Toll and Imd pathways, that control the expression of anti microbial peptides (AMP) genes in response to microbial infection. We have solved the structure of recognition proteins (PGRP and GNBP) upstream of these two pathways.


UNK
Beatrice AMIGUES
Christian CAMBILLAU
Hervé DARBON
Aline DESMYTER
Yoan DUHOO
UNK
Olivier FUCHSBAUER
UNK
Anais GAUBERT
UNK
Magali GRANGE
Christine KELLENBERGER
Hang LE
Philippe LEONE
Van Son NGUYEN
UNK
Trinh NHUNG
Laurie RAMOND
Jennifer ROCHE
Alain ROUSSEL
Silvia SPINELLI
Jeremy TURCHETTO
Renaud VINCENTELLI

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