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Host-pathogen interactions

Head Alain ROUSSEL

Latest Publications

  1. A new non-classical fold of varroa odorant-binding proteins reveals a wide open internal cavity (2021) Amigues B, Zhu J, Gaubert A, Arena S, Renzone G, Leone P, Fischer IM, Paulsen H, Knoll W, Scaloni A, Roussel A, Cambillau C, Pelosi P. Sci Rep 11 13172 PMID:34162975
  2. Characterization of the First Virulent Phage Infecting Oenococcus oeni, the Queen of the Cellars (2021) Philippe C, Chaib A, Jaomanjaka F, Claisse O, Lucas PM, Samot J, Cambillau C, Le Marrec C. Front Microbiol 11 596541 PMID:33519734
  3. Wine Phenolic Compounds Differently Affect the Host-Killing Activity of Two Lytic Bacteriophages Infecting the Lactic Acid Bacterium Oenococcus oeni (2020) Philippe C, Chaib A, Jaomanjaka F, Cluzet S, Lagarde A, Ballestra P, Decendit A, Petrel M, Claisse O, Goulet A, Cambillau C, Le Marrec C. Viruses 12 1316 PMID:33213034
  4. Structure of odorant binding proteins and chemosensory proteins determined by X-ray crystallography (2020) Gaubert A, Amigues B, Spinelli S, Cambillau C. Methods Enzymol 642 151-167 PMID:32828251
  5. Structural Insights into Lactococcal Siphophage p2 Baseplate Activation Mechanism (2020) Spinelli S, Tremblay D, Moineau S, Cambillau C, Goulet A. Viruses 12 E878 PMID:32796652
  6. The CWPS Rubik's cube: Linking diversity of cell wall polysaccharide structures with the encoded biosynthetic machinery of selected Lactococcus lactis strains (2020) Mahony J, Frantzen C, Vinogradov E, Sadovskaya I, Theodorou I, Kelleher P, Chapot-Chartier MP, Cambillau C, Holo H, van Sinderen D. Mol Microbiol 114 582-596 PMID:32515029
...All publications

Our group aims at understanding and analyzing the interactions of pathogens with their host, leading to its infection and its possible destruction. Our targets of interest are diverse, they gather i) bacteriophages infecting, among others, the lactic acid bacterium Lactococcus lactis used in the dairy industry, ii) bacterial secretion systems of type 6 and type 9, iii) the immune innate response of Drosophila after microbial or viral infection.

Understanding of host-pathogen interactions is at the heart of fighting pathogens involved in infectious diseases (bacteria, viruses). During the last ten years, we studied the structure and adhesion mechanism of bacteriophages (viruses that infect bacteria) infecting the bacterium used in the milk industry Lactococcus lactis. We have determined the structures of several phage virions by electron microscopy, and of the anti-receptor or the baseplate (1-2 MDa), eventually complexed to the receptor, by X-ray diffraction. We found that some phages, but not all, require an activation step by the calcium ion. More recently, we have addressed the study of type VI (T6SS) and Type IX (T9SS) secretion systems. We determined the structure and interactions of some of the 13 components of the T6SS, and we pursue the study of the system, including the trans-membrane domains. We wish to reconstruct the whole system by a "hybrid" approach, including electron microscopy and diffraction. The same approach is applied to T9SS. Finally, we have chosen the Drosophila model organism to study the innate immune response of the host. Genetic analysis has delineated two major regulatory pathways, the Toll and Imd pathways, that control the expression of anti microbial peptides (AMP) genes in response to microbial infection. We have solved the structure of recognition proteins (PGRP and GNBP) upstream of these two pathways.

Allele-Boubou COULIBALY
Adeline GOULET
Isabelle IMBERT
Philippe LEONE

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