CNRS - AIX MARSEILLE UNIV: UMR7257

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Host-pathogen interactions

Head Alain ROUSSEL

Latest Publications

  1. Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex. (2017) Nguyen VS, Logger L, Spinelli S, Legrand P, ORCID: http://orcid.org/0000-0003-2431-2255, Huyen Pham TT, Nhung Trinh TT, Cherrak Y, Zoued A, ORCID: http://orcid.org/0000-0002-6973-1755, Desmyter A, Durand E, Roussel A, Kellenberger C, Cascales E, ORCID: http://orcid.org/0000-0003-0611-9179, Cambillau C. Nat Microbiol 2 17103 PMID:28650463
  2. Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis. (2017) Duhoo Y, Roche J, Trinh TTN, Desmyter A, Gaubert A, Kellenberger C, Cambillau C, Roussel A, Leone P. Acta Crystallogr F Struct Biol Commun 73 286-293 PMID:28471361
  3. Genetic and functional characterisation of the lactococcal P335 phage-host interactions. (2017) Mahony J, Oliveira J, Collins B, Hanemaaijer L, Lugli GA, Neve H, Ventura M, Kouwen TR, Cambillau C, van Sinderen D. BMC Genomics 18 146 PMID:28183268
  4. Evolved distal tail carbohydrate binding modules of Lactobacillus phage J-1: a novel type of anti-receptor widespread among lactic acid bacteria phages. (2017) Dieterle ME, Spinelli S, Sadovskaya I, Piuri M, Cambillau C. Mol Microbiol in press PMID:28196397
  5. Characterization of the Porphyromonas gingivalis Type IX Secretion Trans-Envelope PorKLMNP Core Complex. (2017) Vincent MS, Canestrari MJ, Leone P, Stathopoulos J, Ize B, Zoued A, Cambillau C, Kellenberger C, Roussel A, Cascales E. J Biol Chem 292 3252-3261 PMID:28057754
  6. Probing Conformational Changes and Interfacial Recognition Site of Lipases With Surfactants and Inhibitors. (2017) Mateos-Diaz E, Amara S, Roussel A, Longhi S, Cambillau C, Carriere F. Methods Enzymol 583 279-307 PMID:28063495
...All publications

Our group aims at understanding and analyzing the interactions of pathogens with their host, leading to its infection and its possible destruction. Our targets of interest are diverse, they gather i) bacteriophages infecting, among others, the lactic acid bacterium Lactococcus lactis used in the dairy industry, ii) bacterial secretion systems of type 6 and type 9, iii) the immune innate response of Drosophila after microbial or viral infection.

Understanding of host-pathogen interactions is at the heart of fighting pathogens involved in infectious diseases (bacteria, viruses). During the last ten years, we studied the structure and adhesion mechanism of bacteriophages (viruses that infect bacteria) infecting the bacterium used in the milk industry Lactococcus lactis. We have determined the structures of several phage virions by electron microscopy, and of the anti-receptor or the baseplate (1-2 MDa), eventually complexed to the receptor, by X-ray diffraction. We found that some phages, but not all, require an activation step by the calcium ion. More recently, we have addressed the study of type VI (T6SS) and Type IX (T9SS) secretion systems. We determined the structure and interactions of some of the 13 components of the T6SS, and we pursue the study of the system, including the trans-membrane domains. We wish to reconstruct the whole system by a "hybrid" approach, including electron microscopy and diffraction. The same approach is applied to T9SS. Finally, we have chosen the Drosophila model organism to study the innate immune response of the host. Genetic analysis has delineated two major regulatory pathways, the Toll and Imd pathways, that control the expression of anti microbial peptides (AMP) genes in response to microbial infection. We have solved the structure of recognition proteins (PGRP and GNBP) upstream of these two pathways.


UNK
Beatrice AMIGUES
Christian CAMBILLAU
Hervé DARBON
Vincent DELAUZUN
Aline DESMYTER
Yoan DUHOO
UNK
Olivier FUCHSBAUER
UNK
Anais GAUBERT
UNK
Magali GRANGE
Christine KELLENBERGER
Philippe LEONE
Van Son NGUYEN
UNK
Trinh NHUNG
Laurie RAMOND
Jennifer ROCHE
Alain ROUSSEL
Silvia SPINELLI
Renaud VINCENTELLI

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