CNRS - AIX MARSEILLE UNIV: UMR7257

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Structural Biology

Biophysical techniques

Head Maria MATE

This biophysical facility offers a large panel of state-of-the-art equipment for the characterization of molecular complexes and the screening or analysis of molecular interactions, in solution or on solid support. These tools are particularly adapted to the study of various complexes such as:

  • protein/protein
  • protein/nucleic acid
  • protein/small molecule
  • protein/detergent

Accessibility

Academics and industrials

How to access ?

Complete the proposal from
http://frisbi.eu/submit-a-proposal/
and check “Marseille” from the “Biophysical facility” item
Contact at AFMB: Maria Mate

Specific equipement

  • Isothermal Titration Calorimetry (ITC): MicroCal iTC200 (Malvern Panalytical)
  • Microscale thermophoresis (MST): Monolith NT.115 (NanoTemper Technologies GmbH)
  • Size exclusion chromatography/multi-angle light scattering/ refractometry (SEC-MALS) :
    HPLC Ultimate 3000 (Thermo Scientific)/ Dawn 8 (Wyatt)/ Optilab (Wyatt)
  • Bio-layer Interferometry (BLI) : Octet RED96 et Blitz (ForteBio, Molecular Devices)
  • Fluorescence-detection Size exclusion chromatography (FSEC): HPLC Dionex/ Fluorescence detector (Thermo Scientific)
  • Nano differential scanning fluorimetry (nanoDSF): Tycho (NanoTemper Technologies GmbH)
  • Dynamic light scattering (DLS): Zetasizer (Malvern Panalytical)
  • Circular dichroism (CD): J-810 (Jasco)

Cost

To be defined from the service requested.

Publications

  • Fuchsbauer O, et al. (2019) Cas9 Allosteric Inhibition by the Anti-CRISPR Protein AcrIIA6. Mol Cell. 2765(19):30697-5.
  • Bauer L, et al. (2019) Fluoxetine Inhibits Enterovirus Replication by Targeting the Viral 2C Protein in a Stereospecific Manner. ACS Infect Dis. 5 (9):1609-1623
  • Tsika AC, et al. (2019) Deciphering the Nucleotide and RNA Binding Selectivity of the Mayaro Virus Macro Domain. J Mol Biol. 431(12):2283-2297
  • Nguyen PC, et al. (2018) Biochemical and Structural Characterization of TesA, a Major Thioesterase Required for Outer-Envelope Lipid Biosynthesis in M. tuberculosis. J Mol Biol 430: 5120-5136
  • Nguyen VS, et al. (2018) Towards a complete structural deciphering of Type VI secretion system. Curr Opin Struct Biol 49 77-84
  • Leone P, et al. (2018) Type IX secretion system PorM and gliding machinery GldM form arches spanning the periplasmic space. Nat Commun 9 429
  • Nguyen VS, et al. (2017) Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex. Nat Microbiol 2 17103
  • Ravachol J, et al. (2016) Mechanisms involved in xyloglucan catabolism by the cellulosome-
  • Foos N, et al. (2015) A flexible extension of the Drosophila ultrabithorax homeodomain defines a novel Hox/PBC interaction mode. Structure 23(2):270-9
  • De la Cruz MA, et al. (2013) A toxin-antitoxin module of Salmonella promotes virulence in mice. PLoS Pathog. 12:e1003827
  • Mate MJ, et al. (2012) Crystal structure of the DNA-bound VapBC2 antitoxin/toxin pair from Rickettsia felis. Nucleic Acids Res 40 3245-58
  • Douzi B, et al. (2011) Deciphering the Xcp Pseudomonas aeruginosa type II secretion machinery through multiple interactions with substrates. J Biol Chem. 40792-801
  • Felisberto-Rodrigues C, et al. (2011) Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathog. 7 e1002386
  • Habchi J, et al. (2011) Characterization of the interactions between the nucleoprotein and the phosphoprotein of Henipavirus. J Biol Chem. 13583-602
  • Sciara G et al (2010) Structure of lactococcal phage p2 baseplate and its mechanism of activation. Proc Natl Acad Sci USA. 107 6852-7
  • Veesler D, et al. (2009) Production and biophysical characterization of the CorA transporter from Methanosarcina mazei. Anal Biochem. 388 115-21.
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