CNRS - AIX MARSEILLE UNIV: UMR7257

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Pascale Marchot, "Structural Glycobiology and Neurobiology" team

Head Pascale MARCHOT

Latest Publications

  1. Chatonnet A, Lenfant N, Marchot P , Selkirk ME
    J Neurochem in press
  2. Kessler P, Marchot P , Silva M, Servent D
    J Neurochem in press
  3. Molgo J, Marchot P , Araoz R, Benoit E, Iorga BI, Zakarian A, Taylor P, Bourne Y, Servent D
    J Neurochem in press
  4. Lenfant N, Bourne Y, Marchot P , Chatonnet A
    Chem Biol Interact 259 343-351
  5. Bourne Y, Sharpless KB, Taylor P, Marchot P
    J Am Chem Soc 138 1611 - 1621

Interests

My research focuses on the function, recognition properties and structure of receptors, enzymes and adhesion molecules involved in central and peripheral neurotransmission, with particular interest for their specificity of ligand recognition, structure and motion dynamics.

Fields of investigation: Biochemistry - Molecular toxinology, pharmacology and enzymology - Reactivity and structure of biological molecules - Molecular neuroscience and neurobiology - Physiology, pathology, and aging of the synapse.

Career

- 2014 - current: co-PI of team "Structural Neurobiology and Glycobiology", AFMB lab, Marseille
- 2013 - 2016: French coordinator of the International Associated Laboratory "SGIDSTS" (CNRS/AMU/UCSD) (US coordinator: Palmer Taylor)
- 2012 - 2013: PI of theme "Structural Neurobiology", team "Structural Neurobiology and Glycobiology", lab "Architecture et Fonction des Macromolécules Biologiques" (AFMB), Marseille
- 2008 - 2011: PI of team "Toxines Animales et Cibles Macromoleculaires" (ToxCiM), lab CRN2M, Marseille
- 2000 - 2007: PI of team "Réactivité et Structure de Macromolécules Biologiques", lab "Ingénierie des Protéines"/"BIMC", Marseille

- 2012 - current: Permanent CNRS Researcher, lab "Architecture et Fonction des Macromolécules Biologiques" (AFMB), Marseille
- 1996 - 2011: Permanent CNRS Researcher, labs "Ingénierie des Protéines", then "Biologie des Interactions Moléculaires et Cellulaires" (BIMC), then "Centre de Recherches en Neurobiologie et Neurophysiologie de Marseille" (CRN2M), Marseille (same lab, different names).
- 1994 - 1995: Permanent CNRS Researcher, sabbatical 2 yrs at the Dept Pharmacology, UCSD, La Jolla CA (USA)
- 1989 - 1993: Permanent CNRS Researcher, lab "Biochimie - Ingénierie des Protéines", Marseille
- 1987 - 1988: Post-doc, lab "Biochimie - Ingénierie des Protéines", Marseille
- 1983 - 1986: DEA + PhD thesis Sp. Neurosciences Moléculaires, lab "Biochimie", Marseille

Univ Diploma

- 1999 - Habilitation à Diriger les Recherches, Univ Méditerranée, Marseille "Fasciculines et acétylcholinestérase, une histoire d’atomes crochus".
- 1986 - PhD Univ Aix-Marseille II, sp Neurosciences, Marseille "Contribution à l’étude de la purification et du mode d’action des toxines des venins de serpents Elapidae" .

Awards

- 2017: Trophy of Attractivness - Congress by the Ville de Marseille, for having organized the XVth ISCM in 2016
- 2016: B.P. Doctor Memorial Young Investigator Travel Award, honoris causa
- 2013-2016: Prime d’Excellence Scientifique (PES) du CNRS

Councils & Committees

- J. Biol. Chem., editorial board member, 2016-2021
- XIV-th ISCM (2013), 12-th ChE (2015), XV-th ISCM (2016): Organizing Committees & International Advisory Boards, elected member, 2012 - current.
- GTBio 2014, scientific committee, solicited member.
- Société Française pour l’Etude des Toxines (SFET), Scientific, Administrative, Meeting organization & Edition Committees, elected member, 2007 – current.
- Vaincre la Mucoviscidose (VLM, ex-AFLM), Scientific Committee, elected member, 2002 - 2008.

Memberships

- Club -M- Ambassadeurs de la Ville de Marseille: 2017
- International Society for Neurochemistry (ISN): 2015 - current
- Association pour les Femmes en Science et en Ingénierie (AFSI): 2015 - current
- Association de Cristallographie d’Aix-Marseille (ACAM): 2013 - current
- Society for Neuroscience (SfN): 2013 - current
- Association Française de Cristallographie (AFC): 2012 - current
- American Society for Biochemistry & Molecular Biology (ASBMB): 2012 - 2021 (Adhering Body of the International Union of Biochemistry and Molecular Biology (IUBMB))
- Société des Neurosciences (SN), 2011 - current (Adhering Body of the International Brain Research Organization (IBRO) and the Federation of European Neurosciences (FENS))
- International Society on Toxinology (IST): 2007 - current
- Association pour la Recherche sur la Sclérose Latérale Amyotrophique et autres maladies du motoneurone (ARSla): 2007 - current
- Société Française pour l’Etude des Toxines (SFET): 1999 - current
- Association pour l’Etude des Cholinestérases: 1996 - current
- National Geographic Society USA: 1994 - 2008

On-going collaborations

- AgroM Différenciation Cellulaire et Croissance (INRA) & Unité Informatique de Centre INRA, Montpellier, France
- Lab Neurobiologie Cellulaire et Moléculaire, CNRS, Institut Fédératif de Neurobiologie Alfred Fessard, Gif-sur-Yvette, France
- Lab Toxinologie Moléculaire, Service d’Ingénierie Moléculaire des Protéines, Institut de biologie et de technologies de Saclay, CEA, Gif-sur-Yvette, France
- Institut Interdisciplinaire de Neuroscience, CNRS/Univ Bordeaux2, Centre de Génomique Fonctionnelle, Bordeaux, France
- BioXtal, campus Luminy, Marseille, France
- Lab Synapse Biology, VIB Center for the Biology of Disease, KU Leuven, Center for Human Genetics, Leuven, Belgium
- Skaggs School of Pharmacy & Pharmaceutical Sciences, Dept of Pharmacology, Univ of California at San Diego, La Jolla, CA, USA
- Dept of Neuroscience & Cell Biology, UMDNJ-Robert Wood Johnson Medical School, Child Health Institute of New Jersey, New Brunswick, NJ, USA
- Dept of Chemistry & Biochemistry, Univ of California at Santa Barbara, Santa Barbara, CA, USA
- Institut NeuroMyoGene, Univ Claude Bernard Lyon 1, CNRS/INSERM, Villeurbanne

Financial supports

- 1995 (9 mths): EMBO, complementary grant for studying abroad
- 1997-1999 (3 yrs): ACI CNRS-NSF, P Marchot & P Taylor (UCSD, La Jolla, CA) coordinators.
- 1997-1999 (3 yrs): AFM, P Marchot & PE Bougis (CNRS Marseille) coordinators.
- 2000-2002 (3 yrs): PAI France-Germany PROCOPE, P Marchot & O Pongs (ZMNH, Hambourg) coordinators; MF Martin-Eauclaire & PE Bougis (CNRS Marseille) contributors.
- 2001-2002 (2 yrs): AFM, P Marchot coordinator.
- 2003-2004 (2 yrs): Programme Bioinformatique inter EPST, P Marchot & A Chatonnet (INRA Montpellier) coordinators.
- 2003-2004 (2 yrs): AFM, P Marchot coordinators.
- 2005-2007 (3 yrs): Action CNRS/USA, P Marchot & P Taylor (UCSD, La Jolla, CA) coordinators.
- 2005 (1 yr): AFM, P Marchot coordinator.
- 2006-2008 (3 yrs): GIP-ANR Santé-environnement - Santé-travail, M Weill (CNRS Montpellier) coordinator; P Marchot, Y Bourne (CNRS Marseille), T Lenormand (CNRS Montpellier) partners.
- 2006-2009 (48 mths): Project Intégré Structural Proteomics in Europe SPINE2-COMPLEXES & TEACH-SG, Work Package WP1.3.1, partner 14 Y Bourne (CNRS Marseille) coordinator; P Marchot deputy.
- 2009 (1 yr): PEPS INSB-CNRS, MF Martin-Eauclaire (CNRS Marseille) coordinator; P Marchot, PE Bougis, B Céard participants.
- 2010-2012 (3 yrs): PICS CNRS, P Marchot & Y Bourne coordinators; P Taylor (UCSD, La Jolla, CA) partner.
- 2011-2014 (45 mths): ANR-Blanc SVSE 2 - Biologie cellulaire & développement, P Marchot coordinator; Y Bourne (CNRS Marseille) & A Chatonnet (INRA Montpellier) partners.
- 2013-2016 (4 yrs): CNRS INSB, LIA France-USA SGIDSTS, P Marchot & P Taylor (UCSD, La Jolla, CA) coordinators.
- 2014-2017 (42 mths): ANR-Blanc SVSE 4 – Neurosciences, O Thoumine (CNRS Bordeaux) coordinator; P Marchot & M Sainlos (CNRS Bordeaux) partners.
- 2016-2018 (3 yrs): ANR Générique "Societal Challenge 4.7", J-L Bessereau (U Lyon1 Claude Bernard) coordinator; P Marchot/Y Bourne partners.

LIA SGIDSTS

The agreement for the creation of an International Associated Laboratory (LIA – Laboratoire International Associé) between the laboratory Architecture et Fonction des Macromolécules Biologiques (AFMB) in Marseille, France, and the Palmer Taylor laboratory, Skaggs School of Pharmacy and Pharmaceutical Sciences (SSPPS) in La Jolla, California, has been signed by the French institutions, Centre National de la Recherche Scientifique (CNRS) and Aix-Marseille Université (AMU), and the American institution, University of California San Diego (UCSD), in February-April 2015. This LIA is named Structure-Guided Investigations into Disease States and Therapeutic Strategies (acronym SGIDSTS) and it effectively started January 1st, 2013, for 4 years. It is coordinated on the French side by Pascale Marchot, a CNRS researcher, and on the American side by Palmer Taylor, a UCSD researcher and professor. The activities of the LIA SGIDSTS cover fundamental research in molecular neuropharmacology and structural biochemistry. (See http://www.cnrs.fr/derci/IMG/pdf/ad... pages 1-2 and http://fr.calameo.com/read/00248816... page 7.)

Staff Research Assoc.

- Joan R. Kanter, staff research associate UCSD, 1994-1995
- Marianick Juin, technician CNRS (permanent position), 2000-2005
- Sandrine Conrod, technician CNRS (permanent position), 2005-2011 (now working with another team of the CRN2M)
- Claire Debarnot, study engineer AMU (permanent position), 2010-2011 (now a member of the AFMB lab and our team, since Jan 2017)
- Audrey Blanchard, study engineer, 2011-2014 (now an engineer at Innate Pharma, Marseille)
- Supanee ("Ann") Potisopon, research engineer/postdoc, Oct-Dec 2014 (now a postdoc at Harvard Medical School, Boston, MA)
- Aurélien Assice-Vivoni, study engineer, Sept 2015 (now an alternating project manager for quality/hygiene/security/environment at EDF)
- Julie Baptiste, study engineer, Nov 2015 - Sept 2016 (now an engineer at the Institute of Human Genetics, Montpellier)
- Maria Mate, research engineer CNRS, Oct 2013 - May 2017 (now working with another team of the AFMB lab)
- Marie-Julie Dejardin, study engineer, Oct 2016 - Jun 2017

Students, postdocs, visitors

Trainees

- Rozenn Guegan, Maitrise Biologie Cellulaire et Physiologie, Univ Rennes, Apr-Jun 1998
- Rozenn Guegan, DEA Neurosciences, Univ Méditerranée, Sept-Nov 1998 (abandoned)
- Céline Hamel, BTS Ecole Nationale de Chimie Physique et Biologie, Paris, May-Jul 1999 & March 2000
- Romain Morichon, Licence Biochimie, Univ Franche-Comté, UFR Sciences et Techniques, Besançon, Jun-Aug 2000
- Ludovic Renault, Magistère de l’Univ Rouen (3rd yr) & DEA BBSG, Univ Aix-Marseille, Sept 2001- Jun 2002
- Raja Noufir (now Bonifay), BTS Lycée Pro Marie Curie, Marseille, May-June 2003 & Nov-Dec 2003 (2 x 8 wks)
- Virginie Fabre, 4th yr INSA Lyon, Dept Biosciences, Jun-Jul 2004 (8 wks)
- Audrey Luzergues, 2nd yr ESIL, Dept Génie Biologique et Microbiologie Appliquée, Marseille, Feb-Apr 2005 (8 wks)
- Marion Traverse, 2nd yr ESIL, Dept Génie Biologique et Microbiologie Appliquée, Marseille, Mar-Apr 2006 (8 wks)
- Carole Rosenberger, Master-1 Nutrition - Sécurité Alimentaire, option Biochimie, Univ Méditerranée, Marseille, Apr-Jun 2006 (8 wks)
- Delphine Chaduli, BTS Lycée Pro Marie Curie, Marseille, May-Jun 2006 & Oct-Dec 2006 (2 x 8 wks)
- Grégoire Mondielli, Master-2 Neurosciences, Univ Méditerranée, Jan-Jun 2007
- David Menahem, 2nd yr ESIL, Dept Biotechnologie, Marseille, Feb-Apr 2010 (8 wks)
- Pauline Dressayre, 2nd yr ESIL, Dept Biotechnologie, Marseille, Feb-Apr 2011 (8 wks)
- Julie Prats, 2nd yr ESIL, Dept Biotechnologie, Marseille, Feb-Apr 2012 (8 wks)
- Camille Ribeyre, Master-1 BBSG, Aix-Marseille Univ, Apr-May 2013 (8 wks)
- Florian Mourey, 2nd yr AgroParisTech, Institut des sciences et industries du vivant et de l’environnement, Centre de Paris, Jun-Jul 2014 (8 wks) (co-mentor A Goulet)
- Farida Nezlioui, Master-2 Biochimie Biologie Structurale et Génomique, Aix-Marseille Univ, Jan - Jun 2016 (co-mentor Adeline Goulet)
- Yoann Crétinon, Master-1 Biochimie Biologie Structurale et Génomique, Aix-Marseille Univ, Mar - Apr 2017 (7 wks) (co-mentors A Goulet, S Platsaki)

PhD students

- Fatima Laraba-Djebari, Thesis Univ Aix-Marseille II, sp. Cell biology and microbiology, 1993-1995, co-mentor (thesis mentor: Marie-France Martin-Eauclaire). Now: Dean of the University of Sciences and Technology Houari Boumédiène, Algiers, Algeria.
- Ludovic Renault, Thesis Univ Méditerranée, sp Neurosciences, Sept 2002 - Dec 2005.
Funding: Association France Alzheimer ; Association Française contre les Myopathies ; Programme Bioinformatique inter-EPST.
Then: Postdoc Stahlberg Lab, Molecular & Cellular Biology, College of Biological Sciences, Univ California, Davis, CA, USA, 2006-2009.
Then again: Research associate, Howard Young Lab, Dept Biochemistry, Univ Alberta, Edmonton, Canada, 2009-2012.
Then again: Senior scientific officer, Cancer Research UK, London Research Inst, UK, 2013 - 2015
Now: Research Scientist & Facility Manager, NeCEN, Leiden University, NL, 2016 - ongoing
- Grégoire Mondielli, Thesis Univ Méditerranée, sp. Neurosciences, Sept 2007 – Dec 2011.
Funding: Association Française contre les Myopathies ; CNRS ; Fondation pour la Recherche Médicale.
Then: Research engineer at the CRN2M, team Gliotransmission & synaptopathies, Marseille, 2013 - 2016
Now: Research engineer at the CRN2M, team SIG-NET : SIGnalling in NeuroEndocrine Tumors, 2016 - on going
- Lamia Mebarki, Thesis Aix-Marseille Univ, sp Structural Biology & Biochemistry, Nov 2013 - ongoing
Funding: BioXtal Nov 2013 - Mar 2014; CIFRE-ANRT BioXtal-AFMB Apr 2014 - Mar 2017; BioXtal Apr - June 2017

Postdocs

- Igor Fabrichny, after a PhD Univ Lomonosov Moscow RU (1995-1999) then a 1rst postdoc at the Institute of Biotechnology, Univ Helsinki FI (2000-2005).
Funding: CNRS « Proposition d’accueil pour un post-doctorant au CNRS » (Oct 2005 - Sept 2006); Fondation pour la Recherche Médicale « Accueil de post-doctorant étranger » (Oct 2006 - Sept 2008).
Now: Product Manager, International Biotechnology Center GENERIUM, Volginskiy, RU (2011-ongoing).
- Adeline Goulet, after (i) a PhD Univ Aix-Marseille (AFMB lab, 2006-2009), (ii) a 1rst postdoc at the CBS Montpellier (2009-2010), & (iii) a 2nd postdoc at Birkbeck College, London, UK (2010-2013).
3rd postdoc in our team, funding: Fondation pour la Recherche Médicale (Sept 2013 - Sept 2015).
Now: CNRS Researcher (CR2) in our team, since Oct 2015.
- Semeli Platsaki, after (i) a PhD in Protein Biochemistry at the Institute for Cell and Molecular Biosciences, Newcastle Univ (UK) 2010-2015, (ii) a 1rst postdoc at the Northern Institute for Cancer Research, Newcastle Univ (UK) 2015-2016.
Now: 2nd postdoc in our team, funding: ANR (June 2016 - June 2018).

Long-term visitors

- Sventja von Daake, visitor Dept of Pharmacology, UCSD (Nov 2003 - Oct 2004)
Funding: Pharmacology Education Research Foundation
Then: Research associate, Susan Taylor Lab, UCSD, La Jolla CA
Now: Lab manager, Comoletti Lab, Dept Neuroscience & Cell Biology, UMDNJ-RWMS, Child Health Institute of New Jersey, New Brunswick NJ (Aug 2011 - ongoing).
- Nicolas Lenfant, visitor postdoc from lab Dynamique Musculaire et Métabolisme, INRA, Montpellier (Jun 2011 - Dec 2013)
Funding: ANR
Then: postdoc AFMB, team Glycogenomics (Jan 2014 - Dec 2016)

Other trainees & visitors

- Kael Duprey, trainee UCSD, Jun-Oct 1994 & Jun-Oct 1995 (in the US)
- Claudine N. Prowse, trainee UCSD, Jan 1994 - Dec 1995 (in the US)
- Verena Pollmann, visitor PhD Univ Hambourg (PhD mentor: O. Pongs), Mar 2000 & Jun 2000 (2 & 1 wks)
- Jens Dannenberg, visitor Postdoc Institut fuer Neurale Signalverarbeitung, ZMNH, Hamburg, Oct 2001 (2 wks)
- Scott B. Hansen, visitor PhD UCSD (PhD mentor, Palmer Taylor), Apr-Jun 2004 & Nov-Dec 2004 (7 & 8 wks)
- Ryan Hibbs, visitor Postdoc UCSD, Nov-Dec 2006
- Cécile Tardif & Marion Leblanc, "1ère S", Lycée des Pierres Vives, Carrières-sur-Seine, Dec2013 - Mar2014, by email only!

Original publications

1986

Bougis PE, Marchot P, Rochat H (1986) Characterization of Elapidae snake venom components using optimized reverse-phase high performance liquid chromatographic conditions and screening assays for α-neurotoxin and phospholipase A2 activities. Biochemistry 25, 7235-7243.

1987

Bougis PE, Marchot P, Rochat H (1987) In vivo synergy of cardiotoxin and phospholipase A2 from the Elapid snake Naja mossambica mossambica. Toxicon 25, 427-431.

Martin MF, Rochat H, Marchot P, Bougis PE (1987) Use of high performance liquid chromatography to demonstrate quantitative variation in components of venom from the scorpion Androctonus australis Hector. Toxicon 25, 569-573.

Otting G, Marchot P, Bougis PE, Rochat H, Wüthrich K (1987) Monitoring the purification by high performance liquid chromatography of cardiotoxins from Naja mossambica mossambica using phase-sensitive two dimensional nuclear magnetic resonance. Eur J Biochem 168, 603-607.

1988

Marchot P, Bougis PE, Céard B, Van Rietschoten J, Rochat H (1988) Localization of the toxic site of Naja mossambica mossambica cardiotoxins: small synthetic peptides express an in vivo lethality. Biochem Biophys Res Comm 153, 642-647.

Marchot P, Frachon P, Bougis PE (1988) Selective distinction at equilibrium between the two α-neurotoxin binding sites of Torpedo acetylcholine receptor by microtitration. Eur J Biochem 174, 537-542.

Zeghloul S, Marchot P, Bougis PE, Ronin C (1988) Selective loss of binding sites for the iodinated α-neurotoxin I from Naja mossambica mossambica upon enzymatic deglycosylation of Torpedo electric organ membranes. Eur J Biochem 174, 543-550.

1989

Le Du MH, Marchot P, Bougis PE, Fontecilla-Camps JC (1989) Crystals of fasciculin 2 from green mamba snake venom: preparation and preliminary X-ray analysis. J Biol Chem 264, 21401-21402.

1990

Muniz ZM, Tibbs GR, Marchot P, Bougis PE, Nicholls DG, Dolly JO (1990) Homologues of a K+ channel blocker α-dendrotoxin: characterization of synaptosomal binding sites and their coupling to elevation of cytosolic free calcium concentration. Neurochem Int 16, 105-112.

Gourdou I, Mabrouk K, Harkiss G, Marchot P, Watt N, Hery F, Vigne R (1990) Neurotoxicité chez la souris de portions riches en cystéines des protéines Tat du virus visna et de VIH-1 [en français; résumé en anglais]. C R Acad Sci (Paris) 311, Série III, pp. 149-155.

1992

Le Du MH, Marchot P, Bougis PE, Fontecilla-Camps JC (1992) 1.9 Å resolution structure of fasciculin 1, an anti-acetylcholinesterase toxin from green mamba snake venom. J Biol Chem 267, 22122-22130.

Laraba-Djebari F, Martin-Eauclaire MF, Marchot P (1992) A fibrinogen-clotting serine proteinase from Cerastes cerastes (horned viper) venom with arginine-esterase and amidase activities: purification, characterization and kinetic parameter determination. Toxicon 30, 1399-1410

1993

Marchot P, Khélif A, Ji YH, Mansuelle P, Bougis PE (1993) Binding of 125I-fasciculin to rat brain acetylcholinesterase: the complex still binds diisopropyl fluorophosphate. J Biol Chem 268, 12458-12467.

1995

van den Born HKL, Radić Z, Marchot P, Taylor P, Tsigelny I (1995) Theoretical analysis of the structure of the peptide fasciculin and its docking to acetylcholinesterase. Protein Sci 4, 703-715.

Laraba-Djebari F, Martin-Eauclaire MF, Mauco G, Marchot P (1995) Afaâcytin, an αβ-fibrinogenase from Cerastes cerastes (Horned Viper) venom, activates purified factor X and induces serotonin release from human blood platelets. Eur J Biochem 233, 756-765

Bourne Y, Taylor P, Marchot P (1995) Acetylcholinesterase inhibition by fasciculin: crystal structure of the complex. Cell 83, 503-512.

Taylor P, Radić Z, Hosea NA, Camp S, Marchot P, Berman HA (1995) Structural bases for the specificity of cholinesterase catalysis and inhibition. Toxicol Lett 82-83, 453-458.

1996

Le Du MH, Housset D, Marchot P, Bougis PE, Navaza J, Fontecilla-Camps JC (1996) Crystal structure of fasciculin 2 from green mamba snake venom: evidence for unusual loop flexibility. Acta Cryst D52, 87-92.

Marchot P, Ravelli RBG, Raves ML, Bourne Y, Vellom DC, Kanter J, Camp S, Sussman JL, Taylor P (1996) Soluble monomeric acetylcholinesterase from mouse: expression, purification, and crystallization in complex with fasciculin. Protein Sci 5, 672-679.

1997

Marchot P, Prowse CN, Kanter J, Camp S, Ackermann EJ, Radić Z, Bougis PE, Taylor P (1997) Expression and inhibitory activity of mutants of fasciculin, a peptidic acetylcholinesterase inhibitor from mamba venom. J Biol Chem 272, 3502-3510.

1998

Sugiyama N, Marchot P, Kawanishi C, Osaka H, Molles B, Sine S, Taylor P (1998) Residues at the subunit interfaces of the nicotinic acetylcholine receptor that contribute to α-conotoxin M1 binding. Molec Pharmacol 53, 787-794

Marchot P, Bourne Y, Prowse CN, Bougis PE, Taylor P (1998) Highlights in toxin research - Inhibition of mouse acetylcholinesterase by fasciculin: crystal structure of the complex and mutagenesis of fasciculin. Toxicon 36, 1613-1622

Taylor P, Osaka H, Molles BE, Sugiyama N, Marchot P, Malany S, McArdle JJ, Sine SM, Tsigelny I (1998) Toxins selective for subunit interfaces as probes of nicotinic acetylcholine receptor structure. J Physiol (Paris) 92, 79-83

1999

Bourne Y, Taylor P, Bougis PE, Marchot P (1999) Crystal structure of mouse acetylcholinesterase: a peripheral site-occluding loop in a tetrameric assembly. J Biol Chem 274, 2963-2970

Sentjurc M, Pecar S, Stojan J, Marchot P, Radić Z, Grubič Z (1999) Electron paramagnetic resonance reveals altered topography of the active site gorge of acetylcholinesterase after binding of fasciculin to the peripheral site. Biochem Biophys Acta 1430, 349-358

Bourne Y, Grassi J, Bougis PE, Marchot P (1999) Conformational flexibility of the acetylcholinesterase tetramer revealed by X-ray crystallography. J Biol Chem 274, 30370-30376.

Marchot P (1999) L’interaction fasciculine-acétylcholinestérase [Monographie en français; résumé en anglais]. J Soc Biol 193, 505-508.

2000

Tricaud N, Marchot P, Martin-Eauclaire MF (2000) On the kaliotoxin and dendrotoxin binding sites on rat brain synaptosomes. Toxicon 38, 1749-1758.

2001

Bourne Y, Dannenberg J, Pollmann V, Marchot P, Pongs O (2001) Immunocytochemical localization and crystal structure of human frequenin (neuronal calcium sensor 1). J Biol Chem 276, 11949-11955.

2002

Tai K, Shen T, Henchman RH, Bourne Y, Marchot P, McCammon JA (2002) Mechanism of acetylcholinesterase inhibition by fasciculin: a 5 ns molecular dynamics simulation. J Am Chem Soc 124, 6153-6161.

Geib S, Sandoz G, Mabrouk K, Matavel A, Marchot P, Hoshi T, Villaz M, Ronjat M, Miquelis R, Lévêque C, De Waard M (2002) Use of a purified and functional recombinant Ca2+ channel β4 subunit in surface plasmon resonance studies. Biochem J 364, 285-292.

2003

Bourne Y, Taylor P, Radić Z, Marchot P (2003) Structural insights into ligand interactions at the acetylcholinesterase peripheral anionic site. EMBO J 22, 1-12.

2004

Hotelier T, Renault L, Cousin X, Nègre V, Marchot P, Chatonnet A (2004) ESTHER, the database of the α/β-hydrolase fold superfamily of proteins. Nucleic Ac Res 32, D145-147.

Bourne Y, Kolb HC, Radić Z, Sharpless KB, Taylor P, Marchot P (2004) Freeze-frame inhibitor captures acetylcholinesterase in a unique conformation. Proc Natl Acad Sci USA 101, 1449-1454.

Bourne Y, Hasper AA, Chahinian H, Juin M, de Graaff LH, Marchot P (2004) Aspergillus niger protein EstA defines a new class of fungal esterases within the α/β-hydrolase fold family of proteins. Structure (Camb) 12, 677-687 [Erratum in Structure (Camb) 12, 1545]. (See also Preview “Defining substrate characteristics from 3D structure: perspective on EstA structure” by Schrag JD & Cygler M, Structure (Camb) 12, 521-522.

2005

Legros C, Céard B, Vacher H, Marchot P, Bougis PE, Martin-Eauclaire MF (2005) Expression of the scorpion α-toxin of reference AaH-II and mutants for identification of some key bioactive elements. Biochem Biophys Acta 1723, 91-99.

Bourne Y, Talley TT, Hansen SB, Taylor P, Marchot P (2005) Crystal structure of a Cbtx-AChBP complex reveals essential interactions between snake α-neurotoxins and nicotinic receptors. EMBO J 24, 1512–1522 [Corrigendum in EMBO J 25, 266 (2006)].

Hansen SB, Sulzenbacher G, Huxford T, Marchot P, Taylor P, Bourne Y (2005) Structures of Aplysia AChBP complexes with nicotinic agonists and antagonists reveal distinctive binding interfaces and conformations. EMBO J 24 3635–3646.

Bourne Y, Radić Z, Kolb HC, Sharpless KB, Taylor P, Marchot P (2005) Structural insights into conformational flexibility at the AChE peripheral site and active site gorge [Revue]. Chem Biol Interact 157-158, 159-165.

Renault L, Nègre V, Hotelier T, Cousin X, Marchot P, Chatonnet A (2005) New friendly tools for users of ESTHER, the database of the α/β-hydrolase fold superfamily of proteins. Chem Biol Interact 157-158, 339-343.

Bourne Y, Hasper AA, Chahinian H, Renault L, Juin MA, de Graaff LH, Marchot P (2005) A. niger protein « EstA », perhaps a new electrotactin, defines a new class of fungal esterases within the α/β-hydrolase fold superfamily. Chem Biol Interact 157-158, 395-396.

Renault L, Essono S, Boquet D, Juin M, Grassi J, Bourne Y, Marchot P (2005) Structural insights into AChE inhibition by monoclonal antibodies. Chem Biol Interact 157-158, 397-400.

2006

Bourne Y, Radić Z, Sulzenbacher G, Kim E, Taylor P, Marchot P (2006) Substrate and product trafficking through the active center gorge of acetylcholinesterase analyzed by crystallography and equilibrium binding. J Biol Chem 281, 29256-29267.

Hansen SB, Sulzenbacher G, Huxford T, Marchot P, Bourne Y, Taylor P (2006) Structural characterization of agonist and antagonist-bound acetylcholine-binding protein from Aplysia californica. J Mol Neurosci 30 (1-2), 101-102.

Bourne Y, Hansen SB, Sulzenbacher G, Talley TT, Huxford T, Taylor P, Marchot P (2006) Structural comparison of three crystalline complexes of a peptidic toxin with a synaptic acetylcholine recognition protein. J Mol Neurosci 30 (1-2), 103-104.

Hansen S, Sulzenbacher G, Huxford T, Marchot P, Bourne Y, Taylor P (2006) Conformational states of AChBP revealed by X-ray crystal structures of bound nAChR agonists, antagonists and non-competitive ligands. Acta Pharmacol. Sin 27, 390-390.

2007

Fabrichny IP, Leone P, Sulzenbacher G, Comoletti D, Miller MT, Taylor P, Bourne Y, Marchot P (2007) Structural analysis of the synaptic protein neuroligin and its β-neurexin complex: determinants for folding and cell adhesion. Neuron 56, 979-991. (See also Preview “A Crystal-Clear Interaction: Relating Neuroligin/Neurexin Complex Structure to Function at the Synapse” by Levinson J.N. & El-Husseini A. Neuron 56, 937-939.)

2009

Hibbs RE*, Sulzenbacher G*, Shi J, Talley TT, Conrod S, Kem WR, Taylor P, Marchot P, Bourne Y (2009) Structural determinants for interaction of partial agonists with acetylcholine binding protein and neuronal α7 nicotinic acetylcholine receptor. EMBO J 28, 3040-3051.

2010

Bourne Y, Radić Z, Aráoz R, Talley TT, Benoit E, Servent D, Taylor P, Molgó J, Marchot P (2010) Structural determinants in phycotoxins and AChBP conferring high affinity binding and nicotinic AChR antagonism. Proc Natl Acad Sci USA 107, 6076-6081.

Leone P, Comoletti D, Ferracci G, Conrod S, Garcia SU, Taylor P, Bourne Y, Marchot P (2010) Structural insights into the exquisite selectivity of neurexin/neuroligin synaptic interactions. EMBO J 29, 2461-2471.

Leone P, Comoletti D, Taylor P, Bourne Y, Marchot P (2010) Structure-function relationship of the α/β-hydrolase fold domain of neuroligin: a comparison with acetylcholinesterase [Revue]. Chem Biol Interact 187 49–55.

Hotelier T, Nègre V, Marchot P, Chatonnet A (2010) Insecticide resistance through mutations in cholinesterases or carboxylesterases: data mining in ESTHER database. J Pestic Sci 35, 315-320.

Bourne Y, Radić Z, Taylor P, Marchot P (2010) Conformational remodeling of femtomolar inhibitor-acetylcholinesterase complexes in the crystalline state. J Am Chem Soc 132, 18292-18300.

2012

Marchot P, Chatonnet A (2012) Editorial to the Special Issue on “Hydrolase versus other functions of members of the α/β-hydrolase fold superfamily of proteins”. Protein Peptide Lett 19, 130-131.

Marchot P, Chatonnet A (2012) Enzymatic activity and protein interactions in α/β-hydrolase fold proteins: moonlighting versus promiscuity. In: Special Issue on “Hydrolase versus other functions of members of the α/β-hydrolase fold superfamily of proteins”. Protein Peptide Lett 19, 132-143.

Fabrichny IP, Mondielli G, Conrod S, Martin-Eauclaire MF, Bourne Y, Marchot P (2012) Structural insights into antibody sequestering and neutralizing of Na+-channel α-type modulator from Old World scorpion venom. J Biol Chem 287, 14136-48.

2013

Lenfant N, Hotelier T, Velluet E, Bourne Y, Marchot P, Chatonnet A (2013) ESTHER, the database of the α/β-hydrolase fold superfamily of proteins : tools to explore diversity of functions. Nucleic Acids Res (Database Issue) 41, D423-D429.

Lenfant N, Hotelier T, Bourne Y, Marchot P, Chatonnet A (2013) Proteins with an α/β-hydrolase fold: relationships between subfamilies in an ever-growing superfamily. Chem Biol Interact 203, 266-268.

Martin-Eauclaire MF, Benoit E, Marchot P, Barbier J, Molgó J, Servent D (2013) Editorial to the Special Issue on “Toxins: From Threats to Benefits” (Molgó J, Benoit E, Barbier J, Marchot P, Servent D, Guest Eds), Toxicon 75, 1-2.

Bourne Y*, Renault L*, Essono S, Mondielli G, Lamourette P, Boquet D, Grassi J, Marchot P (2013) Molecular characterization of monoclonal antibodies that inhibit acetylcholinesterase by targeting the peripheral site and backdoor region. PLOS One 8, e77226.

2014

Lenfant N, Hotelier T, Bourne Y, Marchot P, Chatonnet A (2014) Tracking the origin and divergence of cholinesterases and neuroligins: the evolution of synaptic proteins. J Mol Neurosci 53, 362-369.

Bourne Y, Marchot P (2014) The neuroligins and their ligands: from structure to function at the synapse. J Mol Neurosci 53, 387-396.

Benoit E, Mattei C, Barbier J, Marchot P, Molgó J, Servent D (2014) Editorial to the Special Issue on “Freshwater and Marine Toxins” (Molgó J, Benoit E, Barbier J, Marchot P, Servent D, Guest Eds), Toxicon 91, 1-4.

2015

Bourne Y*, Renault L*, Marchot P (2015) Crystal structure of snake venom acetylcholinesterase in complex with inhibitory antibody fragment Fab410 bound at the peripheral site: evidence for open and closed states of a back door channel. J Biol Chem 290, 1522–1535.

Bourne Y, Sulzenbacher G, Radić Z, Aráoz R, Reynaud M, Benoit E, Zakarian A, Servent D, Molgó J, Taylor P, Marchot P (2015) Marine macrocyclic imines, pinnatoxins A and G: structural determinants and functional properties to distinguish neuronal α7 from muscle (α1)2βγδ nAChRs. Structure 23, 1106-1115. (See also Preview "From Shellfish Poisoning to Neuroscience" by Shahsavar A and Balle T, Structure 23, 979-980.)

2016

Bourne Y, Sharpless KB, Taylor P, Marchot P (2016) Steric and dynamic parameters influencing in situ cycloadditions to form triazole inhibitors with crystalline acetylcholinesterase. J Am Chem Soc 138, 1611−1621.

Lenfant N, Bourne Y, Marchot P, Chatonnet A (2016) Relationships of human α/β hydrolase fold proteins and other organophosphate-interacting proteins. Chem Biol Interact 259, 343-351.

2017

Kessler P, Marchot P, Silva M, Servent D (2017) The three-finger toxin fold: a multifunctional structural scaffold able to modulate the cholinergic functions. J Neurochem, in press.

Chatonnet A, Lenfant N, Marchot P, Selkirk ME (2017) Natural genomic amplification of cholinesterase genes in animals. J Neurochem, in press.

Molgó J, Marchot P, Aráoz R, Benoit E, Iorga BI, Zakarian A, Taylor P, Bourne Y, Servent D (2017) Cyclic imine toxins from dinoflagellates: a growing family of potent antagonists of the nicotinic acetylcholine receptors. J Neurochem, in press.

Editorial production

Ouvrage Toxines et cancer (Goudey-Perrière F, Benoit E, Goyffon M, Marchot P, eds.) Coll. Rencontres en Toxinologie, Editions Lavoisier, Cachan, 2006 (326 pages) (ISBN: 2-7430-0958-6).

Ouvrage Toxines émergentes: nouveaux risques (Goudey-Perrière F, Benoit E, Marchot P, Popoff M, eds) Coll Rencontres en Toxinologie, Editions Lavoisier, Cachan, 2007 (208 pages) (ISBN: 978-2-7430-1037-9).

Ouvrage électronique (e-book) Toxines et fonctions cholinergiques neuronales et non neuronales (Benoit E, Goudey-Perrière F, Marchot P, Servent D, eds) Coll Rencontres en Toxinologie, Publications de la SFET, Châtenay-Malabry, 2008 (160 pages) (ISSN: 1760-6004).

Ouvrage électronique (e-book) Toxines et signalisation (Benoit E, Goudey-Perrière F, Marchot P, Servent D, eds) Coll Rencontres en Toxinologie, Publications de la SFET, Châtenay-Malabry, 2009 (206 pages) (ISSN: 1760-6004).

Ouvrage électronique (e-book) Advances and new technologies in toxinology (Barbier J, Benoit E, Marchot P, Mattei C, Servent D, eds) Coll Rencontres en Toxinologie, Publications de la SFET, Châtenay-Malabry, 2010 (184 pages) (ISSN: 1760-6004).

Special Issue on Hydrolase versus other functions of members of the α/β-hydrolase fold superfamily of proteins (Chatonnet A, Marchot P, Guest eds). Protein & Peptide Lett, Bentham Science Publishers Ltd. 2012 (ISSN: 0929-8665), vol 19(2), pp 130-197.

Special Issue on Toxins: From Threats to Benefits, 20th Meeting of the French Society of Toxinology (SFET) (Molgó J, Benoit E, Barbier J, Marchot P, Servent D, Guest eds), Toxicon (ISSN: 0041-0101), Elsevier, 2013, vol 75, pp 1-224.

Special Issue on Freshwater and Marine Toxins, 21th Meeting of the French Society of Toxinology (SFET) (Molgó J, Benoit E, Barbier J, Marchot P, Servent D, Guest eds), Toxicon (ISSN: 0041-0101), Elsevier, 2014, vol 91, pp 1-184.

Special Issue on XVth International Symposium on Cholinergic Mechanisms (Prado M, Marchot P, Silman I, Guest eds), J Neurochem (ISSN: 1471-4159), Wiley, 2017, in press.

Book chapters and proceedings

Marchot P, Bougis PE, Céard B, Van Rietschoten J, Rochat H (1989) Lethal synthetic peptides as models of the toxic site of Naja mossambica mossambica cardiotoxins. In Second Forum on Peptides (Aubry A, Marraud M, Vitoux B, eds) Colloque INSERM / John Libbey Eurotext Ltd. 174, 461-464

Le Du MH, Marchot P, Bougis PE, Fontecilla-Camps JC (1993) Fasciculin 1 (Green mamba). In Macromolecular Structures (Hendrickson WA, Wüthrich K, eds) pp. 152-153, Current Biology Ltd, Philadelphia.

Marchot P, Camp S, Radić Z, Bougis PE, Taylor P (1995) Structural determinants of fasciculin specificity for acetylcholinesterase. In Enzymes of the Cholinesterase Family (Quinn DM, Balasubramanian AS, Doctor BP, Taylor P, eds) pp. 197-202, Plenum Publishing Corp., NY.

Bourne Y, Taylor P, Marchot P (1996) Acetylcholinesterase, recombinant monomeric form (mouse) / fasciculin 2 (mamba) complex. In Macromolecular Structures (Hendrickson WA, Wüthrich K, eds) pp. 8-9, Current Biology Ltd, London, UK.

Bourne Y, Taylor P, Kanter JR, Bougis PE, Marchot P (1998) Crystal structure of mouse acetylcholinesterase. In Structure and Function of Cholinesterases and Related Proteins (Doctor BP, Taylor P, Quinn DM, Rotundo RL, Gentry MK, eds) pp. 315-322, Plenum Publishing Corp., NY.

Marchot P, Bourne Y, Prowse CN, Kanter JR, Eads J, Bougis PE, Taylor P (1998) Fasciculin inhibition of mouse acetylcholinesterase: crystal structure of the complex and mutagenesis of fasciculin. In Structure and Function of Cholinesterases and Related Proteins (Doctor BP, Taylor P, Quinn DM, Rotundo RL, Gentry MK, eds) pp. 331-338, Plenum Publishing Corp., NY.

Kanter JR, Eads J, Camp S, Marchot P, Taylor P (1998) Expression and purification of recombinant mutants of fasciculin from mammalian cells. In Structure and Function of Cholinesterases and Related Proteins (Doctor BP, Taylor P, Quinn DM, Rotundo RL, Gentry MK, eds) pp. 240-241, Plenum Publishing Corp., NY.

Marchot P, Bougis PE (2000) The fasciculins, and their interaction with acetylcholinesterase. In: Animal Toxins. Facts and Protocols (Rochat H, Martin-Eauclaire MF, eds) pp. 246-275, Birkhäuser Verlag, Basel.

Bourne Y, Marchot P (2002) Fasciculines et autres ligands de l’AChE: développements structuraux récents [Revue en français; résumé en anglais]. In: Toxines et Recherches Biomédicales (Goudey-Perrière F, Bon C, Puiseux-Dao S, Sauviat MP, eds) pp. 287-296, Coll Rencontres en Toxinologie, Editions Scientifiques et Médicales Elsevier, Paris.

Comoletti D, Flynn R, Jennings L, Hoffman R, Marchot P, Bourne Y, Südhof T, Taylor P (2004) Interaction of recombinant soluble neuroligin-1 with neurexin-1-β. In Cholinergic Mechanisms - function and dysfunction Symposium Proceedings (Silman I, Michaelson DM, Anglister L, Fisher A, Soreq H, eds) pp. 523-525, Taylor & Francis group, UK.

Tai K, Shen T, Henchman RH, Bourne Y, Marchot P, McCammon JA (2004) Mechanism of acetylcholinesterase inhibition by fasciculin: A 5ns molecular dynamics simulation. In Cholinergic Mechanisms - function and dysfunction Symposium Proceedings (Silman I, Michaelson DM, Anglister I, Fisher A, Soreq H, eds) pp. 727-728, Taylor & Francis group, UK.

Bourne Y, Hansen SB, Sulzenbacher G, Talley TT, Huxford T, Taylor P, Marchot P (2005) Comparaison structurale de trois complexes entre une toxine peptidique et une protéine synaptique liant l’acétylcholine. [Revue en français; résumé en anglais]. In Toxines et Douleur (Bon C, Goudey-Perrière F, Goyffon M, Sauviat MP, eds), pp. 213-216, Coll Rencontres en Toxinologie, Editions Lavoisier, Cachan, France.

Fabrichny I, Conrod S, Martin-Eauclaire MF, Devaux C, Bourne Y, Marchot P (2007) Immunothérapie antiscorpionique: approche structurale. [En français; résumé et légendes en anglais]. In Toxines émergentes: nouveaux risques (Goudey-Perrière F, Benoit E, Marchot P, Popoff M, eds), pp. 197-202, Coll. Rencontres en Toxinologie, Editions Lavoisier, Cachan, France.

Bourne Y, Radić Z, Talley TT, Conrod S, Taylor P, Molgó J, Marchot P (2008) Utilisation des « protéines liant l’ACh » (AChBP) en toxinologie structurale : nouveaux exemples. [En français; résumé et légendes en anglais]. In Toxines et fonctions cholinergiques neuronales et non neuronales (Benoit E, Goudey-Perrière F, Marchot P, Servent D, eds), pp. 27-28, Coll. Rencontres en Toxinologie, Publications de la SFET, Châtenay-Malabry, France.

Taylor P, Radić Z, Talley TT, Nemecz A, De Jaco A, Comoletti D, Fabrichny IP, Leone P, Miller MT, Dubi N, Fokin VV, Sharpless KB, Bourne Y, Marchot P (2008) Retrogenomics - Proceeding from gene product back to the gene: applications in drug discovery and uncovering linkages in congenital disorders. [Revue en anglais; résumé et légendes en français]. In Toxines et fonctions cholinergiques neuronales et non neuronales (Benoit E, Goudey-Perrière F, Marchot P, Servent D, eds), pp. 29-32, Coll Rencontres en Toxinologie, Publications de la SFET, Châtenay-Malabry, France.

Douzi B, Geerlof A, Gilles N, Darbon H, Marchot P, Vincentelli R (2010) A new system for expressing recombinant animal toxins in E. coli? In Advances and new technologies in toxinology (Barbier J, Benoit E, Marchot P, Mattei C, Servent D, eds), pp. 149-152, Coll Rencontres en Toxinologie, Publications de la SFET, Châtenay-Malabry, France.

Published abstracts

Bougis PE, Marchot P, Rochat H (1985) Reverse-phase high performance liquid chromatography of Elapidae snake venoms. Toxicon 23, p. 554.

Marchot P, Khélif A, Bougis PE (1985) Brain acetylcholinesterase inhibitor from mamba snake venom: reverse-phase HPLC isolation and characterisation. Toxicon 23, p. 592.

Marchot P, Bougis PE, Rochat H (1987) High performance liquid chromatography of Elapidae snake venoms. Toxicon 25, p. 372.

Laraba-Djebari F, Martin-Eauclaire MF, Marchot P (1993) Two thrombin-like proteinases from Cerastes cerastes (Horn viper) venom: purification, characterization and kinetic parameter determination. Toxicon 31, p. 528.

Laraba-Djebari F, Martin-Eauclaire MF, Marchot P (1994) Afaâcytin, a thrombin-like enzyme from the venom of Cerastes cerastes: structural and functional properties. Toxicon 32, p. 400.

Marchot P, Khélif A, Ji YH, Mansuelle P, Bougis PE (1994) Binding of 125I-fasciculin to rat brain acetylcholinesterase. Toxicon 32, p. 401.

Kanter J, Marchot P, Prowse C, Camp S, Taylor P (1997) Contributions of individual amino acid residues to the stability of the fasciculin-acetylcholinesterase interaction. Pharmacologist 39, p. 104.

Marchot P, Taylor P, Kanter JR, Bougis PE, Bourne Y (1998) Tetrameric assembly and peripheral site-occluding loop of mouse acetylcholinesterase. J Physiol (Paris) 92, p. 465

Bougis PE, Taylor P, Marchot P (1998) Biotinylation of the tyrosine residues of fasciculin. J Physiol (Paris) 92, p. 416.

Marchot P (1999) Acetylcholinesterase inhibition by fasciculin. Toxicon 37, 1209-1210.

Bougis PE, Taylor P, Marchot P (2000) Biotinylated fasciculins: potential tools for histochemical studies of acetylcholinesterase. Toxicon 38, 1637-1638.

Flynn R, Comoletti D, Marchot P, Bourne Y, Südhof T, Taylor P (2003) Interaction of recombinant soluble neuroligins-1 with neurexin-β. FASEB J 17 (4), A640.

Bourne Y, Sulzenbacher G, Radić Z, Aráoz R, Reynaud M, Benoit E, Talley TT, Zakarian A., Taylor P, Servent D, Molgó J, Marchot P (2014) Diversity in the binding interactions of marine toxins to AChBP, the soluble nAChR surrogate. Toxicon 91, 171-172.

Marchot P, Sulzenbacher G, Radić Z, Aráoz R, Reynaud M, Benoit E, Zakarian A, Servent D, Molgó J, Taylor P, Bourne Y (2017) Diversity in the binding interactions of marine phycotoxins to AChBP, the soluble nAChR surrogate. J Neurochem, in press.

Bourne Y, Radić Z, Sharpless KB, Taylor P, Marchot P (2017) Structural and functional analysis of acetylcholinesterase complexes with in situ click-chemistry inhibitors. J Neurochem, in press.

Lenfant N, Selkirk ME, Hotelier T, Bourne Y, Marchot P, Chatonnet A (2017) Evolution of animal cholinesterases. J Neurochem, in press.

Chatonnet A, Marchot P, Bourne Y, Lenfant N, Hotelier T (2017) ESTHER database: update on the alpha/beta hydrolase fold superfamily of proteins. J Neurochem, in press.

Crystal structures

- 1FAS Fasciculin 1 (LeDu, Marchot, Bougis, Fontecilla-Camps, 1993)
- 1FSC Fasciculin 2 (LeDu, Housset, Marchot, Bougis, Navaza, Fontecilla-Camps, 1995)

- 1MAH Mouse AChE, fasciculin 2 complex (Bourne, Taylor, Marchot, 1995)
- 1KU6 Mouse AChE, revisited fasciculin 2 complex (Bourne, Burmeister, Taylor, Marchot, 2002)

- 1C2B Eel AChE, natural tetramer, structure A (Bourne, Marchot, 1999)
- 1C2O Eel AChE, natural tetramer, structure B (id.)

- 1MAA Mouse AChE, crystalline tetramer (Bourne, Taylor, Bougis, Marchot, 1998)

- 1J06 Mouse AChE, first apo form (Bourne, Taylor, Radić, Marchot, 2002)
- 1J07 Mouse AChE, decidium complex (id.)
- 1N5R Mouse AChE, propidium complex (id.)
- 1N5M Mouse AChE, gallamine complex (id.)

- 2H9Y Mouse AChE, complex with TMTFA (Bourne, Radić, Sulzenbacher, Kim, Taylor, Marchot, 2006)
- 2HA0 Mouse AChE, complex with 4K-TMA (id.)
- 2HA2 Mouse AChE, complex with succinyldicholine (id.)
- 2HA3 Mouse AChE, complex with choline (id.)
- 2HA4 Mouse AChE S203A mutant, complex with acetylcholine (id.)
- 2HA5 Mouse AChE S203A mutant, complex with acetylthiocholine (id.)
- 2HA6 Mouse AChE S203A mutant, complex with succinyldicholine (id.)
- 2HA7 Mouse AChE S203A mutant, complex with butyrylthiocholine (id.)

- 1Q83 Mouse AChE, TZ2PA6 syn1 complex (Bourne, Kolb, Radić, Sharpless, Taylor, Marchot, 2003)
- 1Q84 Mouse AChE, TZ2PA6 anti1 complex (id.)

- 5EHN mouse AChE, syn-TZ2PA5 complex (Bourne, Sharpless, Taylor, Marchot, 2016)
- 5EHQ mouse AChE, anti-TZ2PA5 complex (id.)
- 5EHZ mouse AChE, syn-TZ2PA5 complex from a 1:1 syn/anti mixture (id.)
- 5EIA mouse AChE, anti-TZ2PA5 complex from a 1:6 syn/anti mixture (id.)
- 5EIE mouse AChE, TZ2 complex (id.)
- 5EIH mouse AChE, TZ2+PA5 complex (id.)

- 2XUD Mouse AChE Tyr337Ala mutant (Bourne, Radić, Taylor, Marchot, 2010)
- 2XUG Mouse AChE Tyr337Ala mutant, cocrystallized complex with TZ2PA6 anti1 (1-wk) (id.)
- 2XUF Mouse AChE Tyr337Ala mutant, cocrystallized complex with TZ2PA6 anti1 (1-mth) (id.)
- 2XUH Mouse AChE Tyr337Ala mutant, cocrystallized complex with TZ2PA6 anti1 (10-mths) (id)
- 2XUI Mouse AChE Tyr337Ala mutant, cocrystallized complex with TZ2PA6 syn1 (1-wk) (id)
- 2XUJ Mouse AChE Tyr337Ala mutant, cocrystallized complex with TZ2PA6 syn1 (1-mth) (id)
- 2XUK Mouse AChE Tyr337Ala mutant, cocrystallized complex with TZ2PA6 syn1 (10-mths) (id)
- 2XUO Mouse AChE, Tyr337Ala mutant, soaking complex with TZ2PA6 anti1 (id)
- 2XUP Mouse AChE, Tyr337Ala mutant, soaking complex with TZ2PA6 syn1 (id.)
- 2XUQ Mouse AChE, Tyr337Ala mutant, soaking complex with mixed TZ2PA6 anti1/syn1 (id.)

- 2YMX Inhibitory anti-AChE Fab408 (Bourne, Renault, Essono, Mondielli, Lamourette, Bocquet, Grassi, Marchot, 2013)
- 4QWW Fab410-BfAChE complex (Bourne, Renault, Marchot, 2015)

- 1YI5 Lymnaea AChBP, α-cobratoxin complex (Bourne, Talley, Hansen, Taylor, Marchot, 2005)

- 2BYN Aplysia AChBP, apo (Hansen, Sulzenbacher, Huxford, Marchot, Taylor, Bourne, 2005)
- 2BYP Aplysia AChBP, α-conotoxin Im1 complex (id.)
- 2BYQ Aplysia AChBP, epibatidine complex (id.)
- 2BYR Aplysia AChBP, methyllycaconitine complex (id.)
- 2BYS Aplysia AChBP, lobeline complex (id.)

- 2WNL Aplysia AChBP, anabaseine complex (Hibbs, Sulzenbacher, Shi, Talley, Conrod, Kem, Taylor, Marchot, Bourne, 2009)
- 2WNJ Aplysia AChBP, DMXBA complex (id.)
- 2WN9 Aplysia AChBP, 4-OH-DMXBA complex (id.)
- 2WNC Aplysia AChBP, tropisetron complex (id.)

- 2WZY Aplysia AChBP, SPX complex (Bourne, Radic, Aráoz, Talley, Benoit, Servent, Taylor, Molgo, Marchot, 2010)
- 2X00 Aplysia AChBP, GYM complex (id.)

- 4XHE Aplysia AChBP, PnTx-A complex (Bourne, Sulzenbacher, Marchot, 2015)
- 4XK9 Aplysia AChBP, PnTx-G complex (id)

- 3BE8 NL4 (Fabrichny, Leone, Sulzenbacher, Comoletti, Miller, Taylor, Bourne, Marchot, 2007)
- 2VH8 Nrxβ1-NL4 complex (id.)
- 2WQZ Nrxβ1-NL4 complex, aternative refinement (replaces 2VH8) 2009

- 2XB6 Revisited Nrxβ1-NL4 complex (Leone, Comoletti, Ferracci, Conrod, Taylor, Bourne, Marchot, 2010)

- 4AEI Fab4C1 bound with scorpion toxin AahII (Fabrichny, Mondielli, Conrod, Martin-Eauclaire, Bourne, Marchot, 2011)
- 4AEH Fab9C2 in the absence of a bound scorpion toxin AahI (id)

- 1G8I Human frequenin (NCS-1) (Bourne, Dannenberg, Pollmann, Marchot, Pongs, 2000)

- 1UKC Aspergillus niger EstA (Bourne, Hasper, Chahinian, Juin, de Graff, Marchot, 2003)

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« Des protéines neuronales incriminées dans l’autisme observées en trois dimensions », Centre de Ressources Autisme Rhône-Alpes, Revue de presse n°22 : 16/12/07 - 31/12/07 [Jan 2008] http://www.cra-rhone-alpes.org/spip...

F1000Prime - Article Recommendations [Feb 2008] [http://f1000.com/prime/1100169]

« Analyse en 3D de protéines neuronales incriminées dans l’autisme », La Lettre de l’Université de la Méditerranée, n°125 Février 2008 p.25 http://www.univmed.fr/communication...

« Observation en 3D de protéines neuronales incriminées dans l’autisme », Paris Presse Actualité [Feb 2008] http://www.parispresse.eu/__n87678_...

« Observation en 3D de protéines neuronales incriminées dans l’autisme », Journal du CNRS, n°218 Mars 2008

« Des protéines incriminées dans l’autisme vues en 3D », Neurosciences - Découvertes en rafale sur le cerveau [Mar 2008] http://www2.cnrs.fr/presse/journal/...

« Pierres fondamentales », Revue de l’UNAPEI, Septembre 2008

For “Bourne et al., PNAS 2010”:

« Comment agissent certaines toxines accumulées dans les fruits de mer » [Mar 2010] Communiqué de presse du CNRS http://www2.cnrs.fr/presse/communiq...

« Comment agissent certaines toxines accumulées dans les fruits de mer » [Mar 2010] Communiqué de presse du CEA – Sciences du vivant http://www-dsv.cea.fr/la-dsv/toute-...]

”The mode of action of certain toxins that accumulate in seafood” [Mar 2010] Communiqué de presse du CNRS page internationale http://www2.cnrs.fr/en/1709.htm

"Comment des coquillages deviennent toxiques" [Mar 2010] Ma Planète [http://ma-planete.com/blog/view/id_...]

For "Bourne et al., Structure 2015”:

Preview "From Shellfish Poisoning to Neuroscience" by Shahsavar A and Balle T, Structure 23, 979-980 [http://www.ncbi.nlm.nih.gov.gate1.i...].

Cover pictures

cholinesterases Book Structure and Function of Cholinesterases and Related Proteins (Doctor BP, Taylor P, Quinn DM, Rotundo RL, & Gentry MK, eds) Plenum Publishing Corp, NY, 1998 (630 pages) (ISBN: 0-306-46050-5).


toxinologie E-book Toxines et fonctions cholinergiques neuronales et non neuronales (Benoit E, Goudey-Perrière F, Marchot P, Servent D, eds) Coll. Rencontres en Toxinologie, SFET Publications, Châtenay-Malabry, 2008 (160 pages) (ISSN: 1760-6004).


jacs Journal J. Am. Chem. Soc. Vol. 132, Iss. 51, December 29, 2010

Portfolio

Crystal structure of the pentameric complex formed between AChBP, a soluble (...) Crystal structure of the neuroligin dimer (yellow subunits, purple (...) Crystal structure of the dimeric complex formed between acetylcholinesterase, a Crystal structure of the pentameric complex formed between AChBP, a soluble (...)
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