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Structural Biology


The AFMB structural biology facility gathers a state-of-the-art equipement available to a broad scientific community from academic and industrial origins. The facility offers a large panel of complementary techniques and expertise to tackle the tridimensional structure of proteins or macromolecular assemblies from the corresponding genes. The facility represents one node of the French infrastructure for integrated structural biology (FRISBI, aimed at promoting technological transfer between industries and academic labs for technological developments and some therapeutic applications.

High-throughput cloning, expression in bacteria, purification and interaction of recombinant proteins

A high-throughput facility to perform all steps from gene or multi-gene cloning to purified protein samples and to analyze protein-protein/ADN interactions using automated protocols.

Recombinant protein production in eukaryotic systems
Head Claire DEBARNOT

This facility offers the latest technologies for production of soluble, membrane-linked or membrane-embedded eukaryotic proteins from mammalian (HEK, CHO...) or insect (Sf9, S2...) cells.

Biophysical techniques
Head Maria MATE

This service offers a large panel of complementary equipment to characterize molecular complexes and to screen or analyze molecular interactions, in solution or on solid support.

Crystallogenesis and crystallography
Heads Gerlind SULZENBACHER, Philippe CANTAU, VĂ©ronique ZAMBONI

An automated infrastructure for the screening of hundreds of conditions using very small amounts of a biological sample. It ensures the observation of results issuing from crystallization.

Electron microscopy

A service that offers a complementary aproach to x-ray crystallography for the structural characterization of biological systems and large macromolecular assemblies.

Selection and production of camelid VHHs/nanobodies
Heads Alain ROUSSEL, Christian CAMBILLAU

This service offers a set of services ranging from camelid nanobodies selection up to their production and characterization (SPR, BLI, micro-cal).

© AFMB UMR7257  W3C validation