L’objectif du groupe est l’identification, la caractérisation et l’élucidation du rôle fonctionnel de protéines ou régions protéiques intrinsèquement désordonnées dans des systèmes biologiques pertinents en termes de santé humaine.

En particulier, l’équipe s’intéresse aux protéines du complexe réplicatif de virus pathogènes humains majeurs, tels que le virus de la rougeole et le virus Nipah.

Au cours de ces vingt dernières années, le paradigme «structure-fonction » a été remis en cause par la découverte des protéines intrinsèquement désordonnées (IDPs), à savoir des protéines dépourvues de structure secondaire et tertiaire stables en conditions physiologiques de pH et salinité et en l’absence d’un partenaire ou ligand. Cependant, elles sont fonctionnelles et très répandues dans le monde du vivant. Le groupe a joué un rôle clé en découvrant que la nucléoprotéine (N) et la phosphoprotéine (P) du virus de la rougeole possèdent de longues régions désordonnées (allant jusqu’à 300 résidus), et a ensuite étendu ces résultats aux protéines N et P des virus Nipah et Hendra, deux pathogènes humains de classe 4.

Cette découverte ouvre de nombreuses perspectives intéressantes d’un point de vue fondamental mais aussi d’un point de vue thérapeutique. L’originalité et les points forts de ces travaux sont liés à leur multidisciplinarité de par l’intégration des approches bioinformatiques, biochimiques, biophysiques et structurales.

Plus récemment le groupe a commencé à s’intéresser aux phénomènes de séparation et transition de phase médiés par des régions protéiques intrinsèquement désordonnées. Les phénomènes de séparation de phase liquide-liquide (LLPS) constituent un nouveau paradigme en biologie. Ils sont à l’origine de la formation des organites sans membranes mais également des usines virales chez les Mononégavirales. Ces condensats permettent une régulation spatio-temporelle fine des activités cellulaires. Ils peuvent subir une “maturation” et nucléer des fibres de type amyloïde.

Les recherches du groupe s’articulent autours des trois axes suivants:

  1. Séparation ou transition de phase et fibrillation des protéines V et W des virus Nipah et Hendra. Ces protéines jouent un rôle clé dans l’évasion de la réponse immunitaire innée de l’hôte. Les recherches, réalisées dans la cadre du projet ANR HENIPHASE et d’un projet financé par le CEFIPRA (collaboration avec Samrat Mukhopadyay, IISER, Mohali, India), visent à (i) élucider les déterminants moléculaires de la fibrillation de ces protéines, (ii) élucider le rôle fonctionnel de ces phénomènes dans le contexte cellulaire, (iii) déterminer la structure de ces fibres par cryo-EM, et (iv) évaluer le potentiel antiviral d’inhibiteurs de la fibrillation.
  2. Séparation de phase liquide-liquide (LLPS) conduisant à la formation des usines virales chez les paramyxovirus. Les recherches, conduites dans le cadre du projet ANRS PEPR MIE 2023 NIPAH-LISA impliquant au total 8 équipes de recherche, visent à développer des approches antivirales innovantes ciblant la formation et/ou la dynamique des usines virales des virus Nipah, de la rougeole et parainfluenza humain de type 3.
  3. Séparation de phase liquide-liquide (LLPS) par les facteurs de transcription de la famille Hox de la Drosophile (collaboration avec Yacine Graba, IBDM, Marseille dans le cadre du projet Amidex BLANC Rercherche IDRHOX).
Domaines de recherche
  • protéines ou régions protéiques intrinsèquement désordonnées
  • séparation de phase et fibrillation
  • paramyxovirus

Publications

2024
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration

Ketty Tamburrini, Sayo Kodama, Sacha Grisel, Mireille Haon, Takumi Nishiuchi, Bastien Bissaro, Yasuyuki Kubo, Sonia Longhi, Jean-Guy Berrin

Proceedings of the National Academy of Sciences of the United States of America 121 (2024)10.1073/pnas.2319998121

2023
DisProt in 2024: improving function annotation of intrinsically disordered proteins

Maria Cristina Aspromonte, Maria Victoria Nugnes, Federica Quaglia, Adel Bouharoua, Vasileios Sagris, Vasilis Promponas, Anastasia Chasapi, Erzsébet Fichó, Galo Balatti, Gustavo Parisi, Martín González Buitrón, Gabor Erdos, Matyas Pajkos, Zsuzsanna Dosztányi, Laszlo Dobson, Alessio Del Conte, Damiano Clementel, Edoardo Salladini, Emanuela Leonardi, Fatemeh Kordevani, Hamidreza Ghafouri, Luiggi Ku, Alexander Miguel Monzon, Carlo Ferrari, Zsófia Kálmán, Juliet Nilsson, Jaime Santos, Carlos Pintado-Grima, Salvador Ventura, Veronika Ács, Rita Pancsa, Mariane Goncalves Kulik, Miguel Andrade-Navarro, Pedro José Barbosa Pereira, Sonia Longhi, Philippe Le Mercier, Julian Bergier, Peter Tompa, Tamas Lazar, Silvio C E Tosatto, Damiano Piovesan

Nucleic Acids Research (2023)10.1093/nar/gkad928

2023
PED in 2024: improving the community deposition of structural ensembles for intrinsically disordered proteins

Hamidreza Ghafouri, Tamas Lazar, Alessio del Conte, Luiggi G Tenorio Ku, Maria Aspromonte, Pau Bernadó, Belén Chaves-Arquero, Lucia Beatriz Chemes, Damiano Clementel, Tiago Cordeiro, Carlos Elena-Real, Michael Feig, Isabella Felli, Carlo Ferrari, Julie Forman-Kay, Tiago Gomes, Frank Gondelaud, Claudiu Gradinaru, Tâp Ha-Duong, Teresa Head-Gordon, Pétur Heidarsson, Giacomo Janson, Gunnar Jeschke, Emanuela Leonardi, Zi Hao Liu, Sonia Longhi, Xamuel Lund, Maria Macias, Pau Martin-Malpartida, Davide Mercadante, Assia Mouhand, Gabor Nagy, María Victoria Nugnes, José Manuel Pérez-Cañadillas, Giulia Pesce, Roberta Pierattelli, Damiano Piovesan, Federica Quaglia, Sylvie Ricard-Blum, Paul Robustelli, Amin Sagar, Edoardo Salladini, Lucile Sénicourt, Nathalie Sibille, João Teixeira, Thomas Tsangaris, Mihaly Varadi, Pet Er Tompa, Silvio C E Tosatto, Alexander Miguel Monzon

Nucleic Acids Research (2023)10.1093/nar/gkad947

Voir la liste complète des publications

Publications de l'équipe

2024
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration

Ketty Tamburrini, Sayo Kodama, Sacha Grisel, Mireille Haon, Takumi Nishiuchi, Bastien Bissaro, Yasuyuki Kubo, Sonia Longhi, Jean-Guy Berrin

Proceedings of the National Academy of Sciences of the United States of America 121 (2024)10.1073/pnas.2319998121

2023
DisProt in 2024: improving function annotation of intrinsically disordered proteins

Maria Cristina Aspromonte, Maria Victoria Nugnes, Federica Quaglia, Adel Bouharoua, Vasileios Sagris, Vasilis Promponas, Anastasia Chasapi, Erzsébet Fichó, Galo Balatti, Gustavo Parisi, Martín González Buitrón, Gabor Erdos, Matyas Pajkos, Zsuzsanna Dosztányi, Laszlo Dobson, Alessio Del Conte, Damiano Clementel, Edoardo Salladini, Emanuela Leonardi, Fatemeh Kordevani, Hamidreza Ghafouri, Luiggi Ku, Alexander Miguel Monzon, Carlo Ferrari, Zsófia Kálmán, Juliet Nilsson, Jaime Santos, Carlos Pintado-Grima, Salvador Ventura, Veronika Ács, Rita Pancsa, Mariane Goncalves Kulik, Miguel Andrade-Navarro, Pedro José Barbosa Pereira, Sonia Longhi, Philippe Le Mercier, Julian Bergier, Peter Tompa, Tamas Lazar, Silvio C E Tosatto, Damiano Piovesan

Nucleic Acids Research (2023)10.1093/nar/gkad928

2023
PED in 2024: improving the community deposition of structural ensembles for intrinsically disordered proteins

Hamidreza Ghafouri, Tamas Lazar, Alessio del Conte, Luiggi G Tenorio Ku, Maria Aspromonte, Pau Bernadó, Belén Chaves-Arquero, Lucia Beatriz Chemes, Damiano Clementel, Tiago Cordeiro, Carlos Elena-Real, Michael Feig, Isabella Felli, Carlo Ferrari, Julie Forman-Kay, Tiago Gomes, Frank Gondelaud, Claudiu Gradinaru, Tâp Ha-Duong, Teresa Head-Gordon, Pétur Heidarsson, Giacomo Janson, Gunnar Jeschke, Emanuela Leonardi, Zi Hao Liu, Sonia Longhi, Xamuel Lund, Maria Macias, Pau Martin-Malpartida, Davide Mercadante, Assia Mouhand, Gabor Nagy, María Victoria Nugnes, José Manuel Pérez-Cañadillas, Giulia Pesce, Roberta Pierattelli, Damiano Piovesan, Federica Quaglia, Sylvie Ricard-Blum, Paul Robustelli, Amin Sagar, Edoardo Salladini, Lucile Sénicourt, Nathalie Sibille, João Teixeira, Thomas Tsangaris, Mihaly Varadi, Pet Er Tompa, Silvio C E Tosatto, Alexander Miguel Monzon

Nucleic Acids Research (2023)10.1093/nar/gkad947

2023
Condensation of the N-terminal domain of human topoisomerase 1 is driven by electrostatic interactions and tuned by its charge distribution

Greta Bianchi, Marco Mangiagalli, Diletta Ami, Junaid Ahmed, Silvia Lombardi, Sonia Longhi, Antonino Natalello, Peter Tompa, Stefania Brocca

International Journal of Biological Macromolecules 254:127754 (2023)10.1016/j.ijbiomac.2023.127754

2023
Viral amyloids: New opportunities for antiviral therapeutic strategies

Frank Gondelaud, Pierre-Yves Lozach, Sonia Longhi

Current Opinion in Structural Biology 83:102706 (2023)10.1016/j.sbi.2023.102706

2023
The Ustilago maydis AA10 LPMO is active on fungal cell wall chitin

Roseline Assiah Yao, Jean-Lou Reyre, Ketty Tamburrini, Mireille Haon, Olivier Tranquet, Akshay Nalubothula, Saumashish Mukherjee, Sophie Le Gall, Sacha Grisel, Sonia Longhi, Jogi Madhuprakash, Bastien Bissaro, Jean-Guy Berrin

Applied and Environmental Microbiology 89 (2023)10.1128/aem.00573-23

2023
Liaisons dangereuses: Intrinsic Disorder in Cellular Proteins Recruited to Viral Infection-Related Biocondensates

Greta Bianchi, Stefania Brocca, Sonia Longhi, Vladimir N Uversky

International Journal of Molecular Sciences 24:2151 (2023)10.3390/ijms24032151

2023
Per Aspera ad Chaos: Vladimir Uversky’s Odyssey through the Strange World of Intrinsically Disordered Proteins

Prakash Kulkarni, Stefania Brocca, A. Keith Dunker, Sonia Longhi

Biomolecules 13:1015 (2023)10.3390/biom13061015

2023
Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies

Juliet F Nilsson, Hakima Baroudi, Frank Gondelaud, Giulia Pesce, Christophe Bignon, Denis Ptchelkine, Joseph Chamieh, Hervé Cottet, Andrey V Kajava, Sonia Longhi

International Journal of Molecular Sciences 24:399 (2023)10.3390/ijms24010399

2023
Minimum information guidelines for experiments structurally characterizing intrinsically disordered protein regions

Bálint Mészáros, András Hatos, Nicolas Palopoli, Federica Quaglia, Edoardo Salladini, Kim van Roey, Haribabu Arthanari, Zsuzsanna Dosztányi, Isabella C Felli, Patrick D Fischer, Jeffrey C Hoch, Cy M Jeffries, Sonia Longhi, Emiliano Maiani, Sandra Orchard, Rita Pancsa, Elena Papaleo, Roberta Pierattelli, Damiano Piovesan, Iva Pritisanac, Luiggi Tenorio, Thibault Viennet, Peter Tompa, Wim Vranken, Silvio C E Tosatto, Norman E Davey

Nature Methods (2023)10.1038/s41592-023-01915-x

2022
Molecular determinants of fibrillation in a viral amyloidogenic domain from combined biochemical and biophysical studies

Juliet F Nilsson, Hakima Baroudi, Frank Gondelaud, Giulia Pesce, Christophe Bignon, Denis Ptchelkine, Joseph Chamieh, Hervé Cottet, Andrey V Kajava, Sonia Longhi

(2022)10.1101/2022.11.02.514851

2022
Split-GFP Reassembly Assay: Strengths and Caveats from a Multiparametric Analysis

Christophe Bignon, Antoine Gruet, Sonia Longhi

International Journal of Molecular Sciences 23:13167 (2022)10.3390/ijms232113167

2022
Serum IgG levels to Epstein-Barr and measles viruses in patients with multiple sclerosis during natalizumab and interferon beta treatment

Linn Persson Berg, Marcus Eriksson, Sonia Longhi, Ingrid Kockum, Clemens Warnke, Elisabeth Thomsson, Malin Bäckström, Tomas Olsson, Anna Fogdell-Hahn, Tomas Bergström

BMJ Neurology Open 4:e000271 (2022)10.1136/bmjno-2022-000271

2022
Predicting Protein Conformational Disorder and Disordered Binding Sites

Ketty Tamburrini, Giulia Pesce, Juliet Nilsson, Frank Gondelaud, Andrey Kajava, Jean-Guy Berrin, Sonia Longhi

2449:95-147 (2022)10.1007/978-1-0716-2095-3_4

2022
Distribution of Charged Residues Affects the Average Size and Shape of Intrinsically Disordered Proteins

Greta Bianchi, Marco Mangiagalli, Alberto Barbiroli, Sonia Longhi, Rita Grandori, Carlo Santambrogio, Stefania Brocca

Biomolecules 12:561 (2022)10.3390/biom12040561

2022
Experimental Evidence of Intrinsic Disorder and Amyloid Formation by the Henipavirus W Proteins

Giulia Pesce, Frank Gondelaud, Denis Ptchelkine, Juliet F Nilsson, Christophe Bignon, Jérémy Cartalas, Patrick Fourquet, Sonia Longhi

International Journal of Molecular Sciences 23:923 (2022)10.3390/ijms23020923

2022
DisProt in 2022: improved quality and accessibility of protein intrinsic disorder annotation

Federica Quaglia, Bálint Mészáros, Edoardo Salladini, András Hatos, Rita Pancsa, Lucía B Chemes, Mátyás Pajkos, Tamas Lazar, Samuel Peña-Díaz, Jaime Santos, Veronika Ács, Nazanin Farahi, Erzsébet Fichó, Maria Cristina Aspromonte, Claudio Bassot, Anastasia Chasapi, Norman E Davey, Radoslav Davidović, Laszlo Dobson, Arne Elofsson, Gábor Erdős, Pascale Gaudet, Michelle Giglio, Juliana Glavina, Javier Iserte, Valentín Iglesias, Zsófia Kálmán, Matteo Lambrughi, Emanuela Leonardi, Sonia Longhi, Sandra Macedo-Ribeiro, Emiliano Maiani, Julia Marchetti, Cristina Marino-Buslje, Attila Mészáros, Alexander Miguel Monzon, Giovanni Minervini, Suvarna Nadendla, Juliet F Nilsson, Marian Novotný, Christos A Ouzounis, Nicolás Palopoli, Elena Papaleo, Pedro José Barbosa Pereira, Gabriele Pozzati, Vasilis J Promponas, Jordi Pujols, Alma Carolina Sanchez Rocha, Martin Salas, Luciana Rodriguez Sawicki, Eva Schad, Aditi Shenoy, Tamás Szaniszló, Konstantinos D Tsirigos, Nevena Veljkovic, Gustavo Parisi, Salvador Ventura, Zsuzsanna Dosztányi, Peter Tompa, Silvio C E Tosatto, Damiano Piovesan

Nucleic Acids Research 50:D480-D487 (2022)10.1093/nar/gkab1082

2022
Functional benefit of structural disorder for the replication of measles, Nipah and Hendra viruses

Frank Gondelaud, Giulia Pesce, Juliet F. Nilsson, Christophe Bignon, Denis Ptchelkine, Denis Gerlier, Cyrille Mathieu, Sonia Longhi

Essays in Biochemistry 66:915-934 (2022)10.1042/EBC20220045

2021
Insights into the evolutionary forces that shape the codon usage in the viral genome segments encoding intrinsically disordered protein regions

Naveen Kumar, Rahul Kaushik, Chandana Tennakoon, Vladimir N Uversky, Sonia Longhi, Kam y J Zhang, Sandeep Bhatia

Briefings in Bioinformatics 22 (2021)10.1093/bib/bbab145

2021
Identification of a Region in the Common Amino-terminal Domain of Hendra Virus P, V, and W Proteins Responsible for Phase Transition and Amyloid Formation

Edoardo Salladini, Frank Gondelaud, Juliet Nilsson, Giulia Pesce, Christophe Bignon, Maria Grazia Murrali, Roxane Fabre, Roberta Pierattelli, Andrey Kajava, Branka Horvat, Denis Gerlier, Cyrille Mathieu, Sonia Longhi

Biomolecules 11:1324 (2021)10.3390/biom11091324

2021
Structural and dynamics analysis of intrinsically disordered proteins by high-speed atomic force microscopy

Noriyuki Kodera, Daisuke Noshiro, Sujit K Dora, Tetsuya Mori, Johnny Habchi, David Blocquel, Antoine Gruet, Marion Dosnon, Edoardo Salladini, Christophe Bignon, Yuko Fujioka, Takashi Oda, Nobuo N Noda, Mamoru Sato, Marina Lotti, Mineyuki Mizuguchi, Sonia Longhi, Toshio Ando

Nature Nanotechnology 16:181-189 (2021)10.1038/s41565-020-00798-9

2021
PED in 2021: a major update of the protein ensemble database for intrinsically disordered proteins

Tamas Lazar, Elizabeth Martínez-Pérez, Federica Quaglia, András Hatos, Lucía Chemes, Javier Iserte, Nicolás Méndez, Nicolás Garrone, Tadeo Saldaño, Julia Marchetti, Ana Julia Velez Rueda, Pau Bernadó, Martin Blackledge, Tiago Cordeiro, Eric Fagerberg, Julie Forman-Kay, Maria Fornasari, Toby Gibson, Gregory-Neal Gomes, Claudiu Gradinaru, Teresa Head-Gordon, Malene Ringkjøbing Jensen, Edward Lemke, Sonia Longhi, Cristina Marino-Buslje, Giovanni Minervini, Tanja Mittag, Alexander Miguel Monzon, Rohit Pappu, Gustavo Parisi, Sylvie Ricard-Blum, Kiersten Ruff, Edoardo Salladini, Marie Skepö, Dmitri Svergun, Sylvain Vallet, Mihaly Varadi, Peter Tompa, Silvio Tosatto, Damiano Piovesan

Nucleic Acids Research 49:D404-D411 (2021)10.1093/nar/gkaa1021

2021
Comprehensive Intrinsic Disorder Analysis of 6108 Viral Proteomes: From the Extent of Intrinsic Disorder Penetrance to Functional Annotation of Disordered Viral Proteins

Naveen Kumar, Rahul Kaushik, Chandana Tennakoon, Vladimir N Uversky, Sonia Longhi, Kam y J Zhang, Sandeep Bhatia

Journal of Proteome Research 20:2704-2713 (2021)10.1021/acs.jproteome.1c00011

2021
Bioinformatic Analysis of Lytic Polysaccharide Monooxygenases Reveals the Pan-Families Occurrence of Intrinsically Disordered C-Terminal Extensions

Ketty C Tamburrini, Nicolas Terrapon, Vincent Lombard, Bastien Bissaro, Sonia Longhi, Jean-Guy Berrin

Biomolecules 11:1632 (2021)10.3390/biom11111632

2021
Extended disorder at the cell surface: The conformational landscape of the ectodomains of syndecans

Frank Gondelaud, Mathilde Bouakil, Aurélien Le Fèvre, Adriana Erica Miele, Fabien Chirot, Bertrand Duclos, Adam Liwo, Sylvie Ricard-Blum

Matrix Biology Plus 12:100081 (2021)10.1016/j.mbplus.2021.100081

2020
PED in 2021: a major update of the protein ensembledatabase for intrinsically disordered proteins

Tamas Lazar, Elizabeth Martínez-Pérez, Federica Quaglia, András Hatos, Lucía B Chemes, Javier A Iserte, Nicolás A Méndez, Nicolás A Garrone, Tadeo E Saldaño, Julia Marchetti, Ana Julia Velez Rueda, Pau Bernadó, Martin Blackledge, Tiago N Cordeiro, Eric Fagerberg, Julie D Forman-Kay, Maria S Fornasari, Toby J Gibson, Gregory-Neal W Gomes, Claudiu C Gradinaru, Teresa Head-Gordon, Malene Ringkjøbing Jensen, Edward A Lemke, Sonia Longhi, Cristina Marino-Buslje, Giovanni Minervini, Tanja Mittag, Alexander Miguel Monzon, Rohit V Pappu, Gustavo Parisi, Sylvie Ricard-Blum, Kiersten M Ruff, Edoardo Salladini, Marie Skepö, Dmitri Svergun, Sylvain D Vallet, Mihaly Varadi, Peter Tompa, Silvio C E Tosatto, Damiano Piovesan, Rodolfo A Ugalde

Nucleic Acids Research (2020)10.1093/nar/gkaa1021

2020
Predicting substitutions to modulate disorder and stability in coiled-coils

Yasaman Karami, Paul Saighi, Rémy Vanderhaegen, Denis Gerlier, Sonia Longhi, Elodie Laine, Alessandra Carbone

BMC Bioinformatics 21 (2020)10.1186/s12859-020-03867-x

2020
2020
Ensemble description of the intrinsically disordered N-terminal domain of the Nipah virus P/V protein from combined NMR and SAXS

Marco Schiavina, Edoardo Salladini, Maria Grazia Murrali, Giancarlo Tria, Isabella Felli, Roberta Pierattelli, Sonia Longhi

Scientific Reports 10 (2020)10.1038/s41598-020-76522-3

2020
Relevance of Electrostatic Charges in Compactness, Aggregation, and Phase Separation of Intrinsically Disordered Proteins

Greta Bianchi, Sonia Longhi, Rita Grandori, Stefania Brocca

International Journal of Molecular Sciences 21:6208 (2020)10.3390/ijms21176208

2020
Sialic acids rather than glycosaminoglycans affect normal and sickle red blood cell rheology by binding to four major sites on fibrinogen

Frank Gondelaud, Philippe Connes, Elie Nader, Céline Renoux, Romain Fort, Alexandra Gauthier, Philippe Joly, Sylvie Ricard‐blum

American Journal of Hematology (2020)10.1002/ajh.25718

2019
Phase transition and amyloid formation by a viral protein as an additional molecular mechanism of virus-induced cell toxicity

Edoardo Salladini, Claire Debarnot, Vincent Delauzun, Maria Grazia Murrali, Priscila Sutto-Ortiz, Silvia Spinelli, Roberta Pierattelli, Christophe Bignon, Sonia Longhi

(2019)

2019
Conformational footprinting of proteins using a combination of top- down electron transfer dissociation and ion mobility

Albert Konijnenberg, Jinyu Li, Johny Habchi, Marion Dosnon, Giulia Rossetti, Rita Grandori, Sonia Longhi, Paolo Carloni, Frank Sobott

(2019)10.1101/283796

2019
Binding induced folding: Lessons from the kinetics of interaction between NTAIL and XD

Angelo Toto, Francesca Troilo, Lorenzo Visconti, Francesca Malagrinò, Christophe Bignon, Sonia Longhi, Stefano Gianni

Archives of Biochemistry and Biophysics 671:255-261 (2019)10.1016/j.abb.2019.07.011

2019
An arsenal of methods for the experimental characterization of intrinsically disordered proteins – How to choose and combine them?

Antoine Schramm, Christophe Bignon, Stefania Brocca, Rita Grandori, Carlo Santambrogio, Sonia Longhi

Archives of Biochemistry and Biophysics 108055 (2019)10.1016/j.abb.2019.07.020

2019
Understanding Intramolecular Crosstalk in an Intrinsically Disordered Protein

Francesca Troilo, Daniela Bonetti, Christophe Bignon, Sonia Longhi, Stefano Gianni

ACS Chemical Biology 14:337-341 (2019)10.1021/acschembio.8b01055

2019
Regulation of measles virus gene expression by P protein coiled-coil properties

Louis-Marie Bloyet, Antoine Schramm, Carine Lazert, Bertrand Raynal, Maggy Hologne, Olivier Walker, Sonia Longhi, Denis Gerlier

Science Advances 5:eaaw3702 (2019)10.1126/sciadv.aaw3702

2019
Modulation of Measles Virus NTAIL Interactions through Fuzziness and Sequence Features of Disordered Binding Sites

Christophe Bignon, Francesca Troilo, Stefano Gianni, Sonia Longhi

Biomolecules 9:8 (2019)10.3390/biom9010008

2018
Conformational response to charge clustering in synthetic intrinsically disordered proteins

Giulia Tedeschi, Edoardo Salladini, Carlo Santambrogio, Rita Grandori, Sonia Longhi, Stefania Brocca

Biochimica et Biophysica Acta (BBA) - General Subjects 1862:2204-2214 (2018)10.1016/j.bbagen.2018.07.011

2018
Folding Mechanism of the SH3 Domain from Grb2

Francesca Troilo, Daniela Bonetti, Carlo Camilloni, Angelo Toto, Sonia Longhi, Maurizio Brunori, Stefano Gianni

Journal of Physical Chemistry B 122:11166-11173 (2018)10.1021/acs.jpcb.8b06320

2018
Exploration of nucleoprotein α-MoRE and XD interactions of Nipah and Hendra viruses

Xu Xu, Wenting Chu, Xiakun Chu, Liufang Xu, Sonia Longhi, Jin Wang, Xu Shang

Journal of Molecular Modeling 24:113 (2018)10.1007/s00894-018-3643-6

2018
How Robust Is the Mechanism of Folding-Upon-Binding for an Intrinsically Disordered Protein?

Daniela Bonetti, Francesca Troilo, Maurizio Brunori, Sonia Longhi, Stefano Gianni

Biophysical Journal 114:1889-1894 (2018)10.1016/j.bpj.2018.03.017

2018
2018
InSiDDe: A Server for Designing Artificial Disordered Proteins

Antoine Schramm, Philippe Lieutaud, Stefano Gianni, Sonia Longhi, Christophe Bignon

International Journal of Molecular Sciences 19:e91 (2018)10.3390/ijms19010091

2017
Partner-Mediated Polymorphism of an Intrinsically Disordered Protein

Christophe Bignon, Francesca Troilo, Stefano Gianni, Sonia Longhi

Journal of Molecular Biology (2017)10.1016/j.jmb.2017.11.012

2017
Comment la ré-initiation de la transcription est-elle gouvernée chez le virus de la rougeole ?

Louis-Marie Bloyet, Philippe Roche, Sonia Longhi, Denis Gerlier

Médecine/Sciences 33:843-845 (2017)10.1051/medsci/20173310010

2017
How order and disorder within paramyxoviral nucleoproteins and phosphoproteins orchestrate the molecular interplay of transcription and replication

Sonia Longhi, Louis-Marie Bloyet, Stefano Gianni, Denis Gerlier

Cellular and Molecular Life Sciences 74:3091-3118 (2017)10.1007/s00018-017-2556-3

2017
Analyzing the Folding and Binding Steps of an Intrinsically Disordered Protein by Protein Engineering

Daniela Bonetti, Francesca Troilo, Angelo Toto, Maurizio Brunori, Sonia Longhi, Stefano Gianni

Biochemistry 56:3780-3786 (2017)10.1021/acs.biochem.7b00350

2017
Simultaneous quantification of protein order and disorder using NMR spectroscopy

Pietro Sormanni, Damiano Piovesan, Gabriella T Heller, Massimiliano Bonomi, Predrag Kukic, Carlo Camilloni, Monika Fuxreiter, Zsuzsanna Dosztanyi, Rohit Pappu, M Madan Babu, Sonia Longhi, Peter Tompa, A Keith Dunker, Vladimir N Uversky, Silvio C E Tosatto, Michele Vendruscolo

Nature Chemical Biology (2017)10.1038/nchembio.2331

2017
Simultaneous quantification of protein order and disorder

Pietro Sormanni, Damiano Piovesan, Gabriella Heller, Massimiliano Bonomi, Predrag Kukic, Carlo Camilloni, Monika Fuxreiter, Zsuzsanna Dosztanyi, Rohit Pappu, M Madan Babu, Sonia Longhi, Peter Tompa, a Keith Dunker, Vladimir Uversky, Silvio Tosatto, Michele Vendruscolo

Nature Chemical Biology 13:339-342 (2017)10.1038/nchembio.2331

2017
Structural disorder and induced folding within two cereal, ABA stress and ripening (ASR) proteins

Karama Hamdi, Edoardo Salladini, Darragh O'Brien, Sebastien Brier, Alexandre Chenal, Ines Yacoubi, Sonia Longhi

Scientific Reports 7:15544 (2017)10.1038/s41598-017-15299-4

2017
Probing Conformational Changes and Interfacial Recognition Site of Lipases With Surfactants and Inhibitors

Eduardo Mateos Diaz, S, Amara, A Roussel, Sonia Longhi, Christian Cambillau, Frédéric Carrière

Methods in Enzymology 279-307 (2017)

2017
The Folding Pathway of the KIX Domain

Francesca Troilo, Daniela Bonetti, Angelo Toto, Lorenzo Visconti, Maurizio Brunori, Sonia Longhi, Stefano Gianni

ACS Chemical Biology 12:1683-1690 (2017)10.1021/acschembio.7b00289

2017
The Henipavirus V protein is a prevalently unfolded protein with a zinc-finger domain involved in binding to DDB1

Sonia Longhi, Vincent Delauzun, Edoardo Salladini

Molecular BioSystems 13:2254-2267 (2017)10.1039/c7mb00488e

2017
Interfacial Properties of N TAIL , an Intrinsically Disordered Protein

Anaïs Benarouche, Johnny Habchi, Alain Cagna, Ofelia Maniti, Agnès P Girard-Egrot, Jean-François Cavalier, Sonia Longhi, Frédéric Carriere

Biophysical Journal 113:2723-2735 (2017)10.1016/j.bpj.2017.10.010

2017
DisProt 7.0: a major update of the database of disordered proteins

Damiano Piovesan, Francesco Tabaro, Ivan Mičetić, Marco Necci, Federica Quaglia, Christopher J. Oldfield, Maria Cristina Aspromonte, Norman E. Davey, Radoslav Davidović, Zsuzsanna Dosztányi, Arne Elofsson, Alessandra Gasparini, András Hatos, Andrey V. Kajava, Lajos Kalmar, Emanuela Leonardi, Tamas Lazar, Sandra Macedo-Ribeiro, Mauricio Macossay-Castillo, Attila Meszaros, Giovanni Minervini, Nikoletta Murvai, Jordi Pujols, Daniel Barry Roche, Edoardo Salladini, Eva Schad, Antoine Schramm, Beata Szabo, Agnes Tantos, Fiorella Tonello, Konstantinos D. Tsirigos, Nevena Veljković, Salvador Ventura, Wim Vranken, Per Warholm, Vladimir N. Uversky, A.~keith Dunker, Sonia Longhi, Peter Tompa, Silvio C.E. Tosatto

Nucleic Acids Research 45:D219-D227 (2017)10.1093/nar/gkw1056

2016
Modulation of Re-initiation of Measles Virus Transcription at Intergenic Regions by PXD to NTAIL Binding Strength

Louis-Marie Bloyet, Joanna Brunel, Marion Dosnon, Véronique Hamon, Jenny Erales, Antoine Gruet, Carine Lazert, Christophe Bignon, Philippe Roche, Sonia Longhi, Denis Gerlier

PLoS Pathogens 12:e1006058 (2016)10.1371/journal.ppat.1006058

2016
Identification and Structural Characterization of an Intermediate in the Folding of the Measles Virus X Domain

Daniela Bonetti, Carlo Camilloni, Lorenzo Visconti, Sonia Longhi, Maurizio Brunori, Michele Vendruscolo, Stefano Gianni

Journal of Biological Chemistry 291:10886+ (2016)10.1074/jbc.M116.721126

2016
Predicting Conformational Disorder

Philippe Lieutaud, François Ferron, Sonia Longhi

1415:265-299 (2016)10.1007/978-1-4939-3572-7_14

2016
Fuzzy regions in an intrinsically disordered protein impair protein-protein interactions

Antoine Gruet, Marion Dosnon, David Blocquel, Joanna Brunel, Denis Gerlier, Rahul K. Das, Daniela Bonetti, Stefano Gianni, Monika Fuxreiter, Sonia Longhi, Christophe Bignon

FEBS Journal 283:576-594 (2016)10.1111/febs.13631

2016
How disordered is my protein and what is its disorder for? A guide through the “dark side” of the protein universe

Philippe Lieutaud, François Ferron, Alexey V Uversky, Lukasz Kurgan, Vladimir N Uversky, Sonia Longhi

Intrinsically Disordered Proteins 4:e1259708 (2016)10.1080/21690707.2016.1259708

2015
Insights into the Hendra virus N-TAIL-XD complex: Evidence for a parallel organization of the helical MoRE at the XD surface stabilized by a combination of hydrophobic and polar interactions

Jenny Erales, Matilde Beltrandi, Jennifer Roche, Maria Mate, Sonia Longhi

Biochimica et Biophysica Acta Proteins and Proteomics 1854:1038-1053 (2015)10.1016/j.bbapap.2015.04.031

2015
Demonstration of a Folding after Binding Mechanism in the Recognition between the Measles Virus N-TAIL and X Domains

Marion Dosnon, Daniela Bonetti, Angela Morrone, Jenny Erales, Eva Di Silvio, Sonia Longhi, Stefano Gianni

ACS Chemical Biology 10:795-802 (2015)10.1021/cb5008579

2015
Insights into the coiled-coil organization of the Hendra virus phosphoprotein from combined biochemical and SAXS studies

Matilde Beltrandi, David Blocquel, Jenny Erales, Pascale Barbier, Andrea Cavalli, Sonia Longhi

Virology 477:42-55 (2015)10.1016/j.virol.2014.12.029

2015
Molecular Basis for Structural Heterogeneity of an Intrinsically Disordered Protein Bound to a Partner by Combined ESI-IM-MS and Modeling

Annalisa d'Urzo, Albert Konijnenberg, Giulia Rossetti, Johnny Habchi, Jinyu Li, Paolo Carloni, Frank Sobott, Sonia Longhi, Rita Grandori

Journal of The American Society for Mass Spectrometry 26:472-481 (2015)10.1007/s13361-014-1048-z

2015
Assessing Binding Perturbation due to Artificial Vibrational Probe Groups in the Nucleoprotein-Phosphoprotein Complex of the Nipah Virus

Rebecca B. Wai, Shana R. Burstein, Sara K. Hess, Jenny Erales, Sonia Longhi, Casey H. Londergan

Biophysical Journal 108:389A (2015)

2015
Developing a Protocol for Ensemble and Vibrational Probe-Containing Molecular Dynamics Simulations of the Nipah Ntail-XD Complex

Shana R. Burstein, Rebecca B. Wai, Sara K. Hess, Casey H. Londergan, Jenny Erales, Sonia Longhi

Biophysical Journal 108:227A (2015)

2015
Order and Disorder in the Replicative Complex of Paramyxoviruses

Marion Dosnon, Christophe Bignon, Sonia Longhi, Jenny Erales, David Blocquel, Johnny Habchi, Matilde Beltrandi, Antoine Gruet

870:351-381 (2015)10.1007/978-3-319-20164-1_12

2014
Sequence of events in measles virus replication: role of phosphoprotein-nucleocapsid interactions

Joanna Brunel, Damien Chopy, Marion Dosnon, Louis-Marie Bloyet, Patricia Devaux, Erica Urzua, Roberto Cattaneo, Sonia Longhi, Denis Gerlier

Journal of Virology 88:10851-10863 (2014)10.1128/JVI.00664-14

2014
Experimental data on the interactions between a viral pre-molten globule (VPg) and an eukaryotic translation initiation factor (eIF4E)

Thierry Michon, Amandine Barra, Justine Charon, Eugénie Hébrard, Sonia Longhi, Benoît Moury, Jocelyne J. Walter

(2014)

2014
An attempt to experimentally assess the contribution of intrinsic disorder to the virus adaptation

Justine Charon, Amandine Barra, Eugénie Hébrard, Sonia Longhi, Benoît Moury, Jocelyne J. Walter, Thierry Michon

(2014)

2014
Diversification of EPR signatures in site directed spin labeling using a beta-phosphorylated nitroxide

Nolwenn Le Breton, Marlène Martinho, Kuanysh Kabytaev, Jérémie Topin, Elisabetta Mileo, David Blocquel, Johnny Habchi, Sonia Longhi, Antal Rockenbauer, Jérôme Golebiowski, Bruno Guigliarelli, Sylvain R.A. Marque, Valérie Belle

Physical Chemistry Chemical Physics 16:4202-4209 (2014)10.1039/c3cp54816c

2013
Atomic resolution description of the interaction between the nucleoprotein and phosphoprotein of Hendra virus.

Guillaume Communie, Johnny Habchi, Filip Yabukarski, David Blocquel, Robert Schneider, Nicolas Tarbouriech, Nicolas Papageorgiou, Rob W H Ruigrok, Marc Jamin, Malene Ringkjøbing Jensen, Sonia Longhi, Martin Blackledge

PLoS Pathogens 9:e1003631 (2013)

2013
Dissecting Partner Recognition by an Intrinsically Disordered Protein Using Descriptive Random Mutagenesis

Antoine Gruet, Marion Dosnon, Andrea Vassena, Vincent Lombard, Denis Gerlier, Christophe Bignon, Sonia Longhi

Journal of Molecular Biology 425:3495-3509 (2013)10.1016/j.jmb.2013.06.025

2013
Assessing induced folding within the intrinsically disordered C-terminal domain of the Henipavirus nucleoproteins by site directed spin labeling EPR spectroscopy

Marlène Martinho, Johnny Habchi, Zeina El Habre, Léo Nesme, Bruno Guigliarelli, Valérie Belle, Sonia Longhi

Journal of Biomolecular Structure and Dynamics 31:453-471 (2013)10.1080/07391102.2012.706068

2013
Solution conformational features and interfacial properties of an intrinsically disordered peptide coupled to alkyl chains: a new class of peptide amphiphiles

Antonella Accardo, Marilisa Leone, Diego Tesauro, Rosa Aufiero, Anaïs Bénarouche, Jean-François Cavalier, Sonia Longhi, Frederic Carriere, Filomena Rossi

Molecular BioSystems 9:1401 (2013)10.1039/c3mb25507g

2012
Plasticity in Structural and Functional Interactions between the Phosphoprotein and Nucleoprotein of Measles Virus

Yaoling Shu, Johnny Habchi, Stéphanie Costanzo, Andre Padilla, Joanna Brunel, Denis Gerlier, Michael Oglesbee, Sonia Longhi

Journal of Biological Chemistry 287:11951-11967 (2012)10.1074/jbc.M111.333088

2012
Compaction and binding properties of the intrinsically disordered C-terminal domain of Henipavirus nucleoprotein as unveiled by deletion studies

David Blocquel, Johnny Habchi, Antoine Gruet, Stéphanie Blangy, Sonia Longhi

Molecular BioSystems 8:392-410 (2012)10.1039/c1mb05401e

2012
Transcription and replication of Mononegavirales: an original molecular machine

David Blocquel, Jean-Marie Bourhis, Jean Francois J. F. Eleouet, Denis Gerlier, Johnny Habchi, Marc Jamin, Sonia Longhi, Filip Yabukarski

Virologie 16:225-257 (2012)

2011
Dividing To Unveil Protein Microheterogeneities: Traveling Wave Ion Mobility Study.

Frédéric Halgand, J. Habchi, Laetitia Cravello, Marlène Martinho, Bruno Guigliarelli, Sonia Longhi

Analytical Chemistry 19:7306-7315 (2011)

2011
Probing structural transitions in both structured and disordered proteins using site-directed spin-labeling EPR spectroscopy

Sonia Longhi, Valérie Belle, André Fournel, Bruno Guigliarelli, Frédéric Carrière

Journal of Peptide Science 17:315-328 (2011)10.1002/psc.1344

2011
Characterization of the Interactions between the Nucleoprotein and the Phosphoprotein of Henipavirus

Johnny Habchi, Stéphanie Blangy, Laurent Mamelli, Malene Ringkjøbing Jensen, Martin Blackledge, Herve Darbon, Michael Oglesbee, Yaoling Shu, Sonia Longhi

Journal of Biological Chemistry 286:13583-13602 (2011)10.1074/jbc.M111.219857

2010
Conformational analysis of the partially disordered measles virus NTAIL-XD complex by SDSL EPR spectroscopy

A. Kavalenka, I. Urbancic, Valérie Belle, S. Rouger, S. Costanzo, S. Kure, André Fournel, Sonia Longhi, Bruno Guigliarelli, J. Strancar

Biophysical Journal 6:1055-1064 (2010)

2009
Interaction between the C-terminal domains of N and P proteins of measles virus investigated by NMR.

Cedric Bernard, Stéphane Gely, Jean-Marie Bourhis, Xavier Morelli, Sonia Longhi, Hervé Darbon

FEBS Letters 583:1084-9 (2009)10.1016/j.febslet.2009.03.004

2009
Modular Organization of Rabies Virus Phosphoprotein

Francine C.A. Gerard, Euripedes de Almeida Ribeiro Jr, Cedric Leyrat, Ivan Ivanov, Danielle Blondel, Sonia Longhi, Rob W.H. Ruigrok, Marc Jamin

Journal of Molecular Biology 388:978-996 (2009)10.1016/j.jmb.2009.03.061

2009
The interaction between the measles virus nucleoprotein and the Interferon Regulator Factor 3 relies on a specific cellular environment

Matteo Colombo, Jean-Marie Bourhis, Célia Chamontin, Carine Soriano, Stéphanie Villet, Stéphanie Costanzo, Marie M. Couturier, Valérie Belle, André Fournel, Hervé Darbon, Denis Gerlier, Sonia Longhi

Virology Journal 6:59 (2009)10.1186/1743-422X-6-59

2009
Intrinsic disorder in Viral Proteins Genome-Linked: experimental and predictive analyses

Eugénie Hébrard, Yannick Bessin, Thierry Michon, Sonia Longhi, Vladimir N Uversky, François Delalande, Alain van Dorsselaer, Pedro Romero, Jocelyne Walter, Nathalie Declerck, Denis Fargette

Virology Journal 6:23 (2009)10.1186/1743-422X-6-23

2009
Rules Governing Selective Protein Carbonylation

Etienne Maisonneuve, Adrien Ducret, Pierre Khoueiry, Sabrina Lignon, Sonia Longhi, Emmanuel Talla, Sam Dukan

PLoS ONE 4:e7269 (2009)10.1371/journal.pone.0007269

2007
Cytosolic 5'-triphosphate ended viral leader transcript of measles virus as activator of the RIG I-mediated interferon response.

Sébastien Plumet, Florence Herschke, Jean-Marie Bourhis, Hélène Valentin, Sonia Longhi, Denis Gerlier

PLoS ONE 2:e279 (2007)10.1371/journal.pone.0000279

2005
VaZyMolO: a tool to define and classify modularity in viral proteins

François Ferron, Corinne Rancurel, Sonia Longhi, Christian Cambillau, Bernard Henrissat, Bruno Canard

Journal of General Virology 86:743-749 (2005)10.1099/vir.0.80590-0

2004
The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world

Marie-Pierre Egloff, Francois Ferron, Valérie Campanacci, Sonia Longhi, Corinne Rancurel, Hélène Dutartre, Eric J Snijder, Alexander Gorbalenya, Christian Cambillau, Bruno Canard

Proceedings of the National Academy of Sciences of the United States of America 101:3792-3796 (2004)10.1073/pnas.0307877101

2003
Structural genomics of the SARS coronavirus: cloning, expression, crystallization and preliminary crystallographic study of the Nsp9 protein

Valérie Campanacci, Marie-Pierre Egloff, Sonia Longhi, François Ferron, Corinne Rancurel, Aurelia Salomoni, Cécile Durousseau, Fabienne Tocque, Nicolas Brémond, Jessika Dobbe, Eric Snijder, Bruno Canard, Christian Cambillau

Acta Crystallographica Section D: Biological Crystallography 59:1628-1631 (2003)10.1107/S0907444903016779

1998
Exploring hydrophobic sites in proteins with xenon or krypton

Thierry Prangé, Marc Schiltz, Lucile Pernot, Nathalie Colloc'H, Sonia Longhi, William Bourguet, Roger Fourme

Proteins: Structure, Function, and Genetics 30:61-73 (1998)10.1002/(SICI)1097-0134(19980101)30:1<61::AID-PROT6>3.0.CO;2-N

Réseau

National
  • Cyrille Mathieu & Denis Gerlier
    • CIRI, Lyon
  • Pierre-Yves Lozach
    • IVPC, Lyon
  • Andrey Kajava
    • CRBM, Montpellier
  • Patrick Fourquet
    • CRCM, Marseille
  • Joseph Chamieh & Hervé Cottet
    • IBMM, Montpellier
  • Jean-Guy Berrin
    • BBF, Marseille
  • Yacine Graba
    • IBDM, Marseille
  • Pierre-Olivier Vidalain
    • CIRI, Lyon
  • Vincent Lotteau
    • P4 “Jean- Merieux”, Lyon
  • Mustapha Si-Tahar
    • CEPR, Tours
  • Samir Messaoudi
    • CoSMIT, École Polytechnique, Palaiseau
  • Nicolas Tsapis
    • Institut Galien, Paris-Saclay
  • Tâp Ha-Duong
    • Université Paris-Saclay
  • Lou Safra
    • CEVIPOF, Centre de Recherches Politiques de Sciences Po
International
  • Roberta Pierattelli
    • CERM, Florence, IT
  • Stefania Brocca & Rita Grandori
    • University of Milano-Bicocca, IT
  • Vladimir Uversky
    • University of South Florida, US
  • Samrat Mukhopadyay
    • IISER, Mohali, India
  • Malte Drescher & Annalisa Pierro
    • University of Konstanz, Germany
  • Peter Tompa
    • VIB, VUB, Belgium

Financement