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Host-pathogen interactions

Head Alain ROUSSEL

Latest Publications

  1. Towards a complete structural deciphering of Type VI secretion system. (2018) Nguyen VS, Douzi B, Durand E, Roussel A, Cascales E, Cambillau C. Curr Opin Struct Biol 49 77-84 PMID:29414515
  2. Structure-Function Analysis of the C-Terminal Domain of the Type VI Secretion TssB Tail Sheath Subunit. (2018) Douzi B, Logger L, Spinelli S, Blangy S, Cambillau C, Cascales E. J Mol Biol 430 297-309 PMID:29223729
  3. Type IX secretion system PorM and gliding machinery GldM form arches spanning the periplasmic space. (2018) Leone P, Roche J, Vincent MS, Tran QH, Desmyter A, Cascales E, Kellenberger C, Cambillau C, Roussel A. Nat Commun 9 429 PMID:29382829
  4. ANISEED 2017: extending the integrated ascidian database to the exploration and evolutionary comparison of genome-scale datasets. (2017) Brozovic M, Dantec C, Dardaillon J, Dauga D, Faure E, Gineste M, Louis A, Naville M, Nitta KR, Piette J, Reeves W, Scornavacca C, Simion P, Vincentelli R, Bellec M, Aicha SB, Fagotto M, Gueroult-Bellone M, Haeussler M, Jacox E, Lowe EK, Mendez M, Roberge A, Stolfi A, Yokomori R, Brown CT, Cambillau C, Christiaen L, Delsuc F, Douzery E, Dumollard R, Kusakabe T, Nakai K, Nishida H, Satou Y, Swalla B, Veeman M, Volff JN, Lemaire P. Nucleic Acids Res in press PMID:29149270
  5. Reverse chemical ecology: Olfactory proteins from the giant panda and their interactions with putative pheromones and bamboo volatiles. (2017) Zhu J, Arena S, Spinelli S, Liu D, Zhang G, Wei R, Cambillau C, Scaloni A, Wang G, Pelosi P. Proc Natl Acad Sci U S A 114 E9802-E9810 PMID:29078359
  6. Unraveling the Self-Assembly of the Pseudomonas aeruginosa XcpQ Secretin Periplasmic Domain Provides New Molecular Insights into Type II Secretion System Secreton Architecture and Dynamics. (2017) Douzi B, Trinh NTT, Michel-Souzy S, Desmyter A, Ball G, Barbier P, Kosta A, Durand E, Forest KT, Cambillau C, Roussel A, Voulhoux R. MBio 8 e01185-17 PMID:29042493
...All publications

Our group aims at understanding and analyzing the interactions of pathogens with their host, leading to its infection and its possible destruction. Our targets of interest are diverse, they gather i) bacteriophages infecting, among others, the lactic acid bacterium Lactococcus lactis used in the dairy industry, ii) bacterial secretion systems of type 6 and type 9, iii) the immune innate response of Drosophila after microbial or viral infection.

Understanding of host-pathogen interactions is at the heart of fighting pathogens involved in infectious diseases (bacteria, viruses). During the last ten years, we studied the structure and adhesion mechanism of bacteriophages (viruses that infect bacteria) infecting the bacterium used in the milk industry Lactococcus lactis. We have determined the structures of several phage virions by electron microscopy, and of the anti-receptor or the baseplate (1-2 MDa), eventually complexed to the receptor, by X-ray diffraction. We found that some phages, but not all, require an activation step by the calcium ion. More recently, we have addressed the study of type VI (T6SS) and Type IX (T9SS) secretion systems. We determined the structure and interactions of some of the 13 components of the T6SS, and we pursue the study of the system, including the trans-membrane domains. We wish to reconstruct the whole system by a "hybrid" approach, including electron microscopy and diffraction. The same approach is applied to T9SS. Finally, we have chosen the Drosophila model organism to study the innate immune response of the host. Genetic analysis has delineated two major regulatory pathways, the Toll and Imd pathways, that control the expression of anti microbial peptides (AMP) genes in response to microbial infection. We have solved the structure of recognition proteins (PGRP and GNBP) upstream of these two pathways.

Béatrice AMIGUES
Adeline GOULET
Philippe LEONE
Thi Trang Nhung TRINH

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