CNRS - AIX MARSEILLE UNIV: UMR7257

Home page > en > Research > Teams > Host-pathogen interactions

Host-pathogen interactions

Head Alain ROUSSEL

Latest Publications

  1. Identification of Dual Receptor Binding Protein Systems in Lactococcal 936 Group Phages. (2018) Hayes S, Duhoo Y, Neve H, Murphy J, Noben JP, Franz CMAP, Cambillau C, Mahony J, Nauta A, van Sinderen D. Viruses 10 PMID:30486343
  2. Biochemical and Structural Characterization of TesA, a Major Thioesterase Required for Outer-Envelope Lipid Biosynthesis in M. tuberculosis. (2018) Nguyen PC, Nguyen VS, Martin BP, Fourquet P, Camoin L, Spilling CD, Cavalier JF, Cambillau C, Canaan S. J Mol Biol in press PMID:30292819
  3. Characterization of epitope specificities of reference antibodies used for the quantification of the birch pollen allergen Bet v 1. (2018) Brier S, Le Mignon M, Jain K, Lebrun C, Peurois F, Kellenberger C, Bordas-Le Floch V, Mascarell L, Nony E, Moingeon P. Allergy 73 1032-1040 PMID:29171882
  4. Functional Carbohydrate Binding Modules Identified in evolved Dits from Siphophages Infecting various Gram-positive Bacteria. (2018) Hayes S, Vincentelli R, Mahony J, Nauta A, Ramond L, Lugli GA, Ventura M, van Sinderen D, Cambillau C. Mol Microbiol in press PMID:30204278
  5. Exploiting the S4-S5 Specificity of Human Neutrophil Proteinase 3 to Improve the Potency of Peptidyl Di(chlorophenyl)-phosphonate Ester Inhibitors: A Kinetic and Molecular Modeling Analysis. (2018) Guarino C, Gruba N, Grzywa R, Dyguda-Kazimierowicz E, Hamon Y, Legowska M, Skorenski M, Dallet-Choisy S, Marchand-Adam S, Kellenberger C, Jenne DE, Sienczyk M, Lesner A, Gauthier F, Korkmaz B. J Med Chem 61 1858-1870 PMID:29442501
  6. Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins. (2018) Hynes AP, Rousseau GM, Agudelo D, Goulet A, Amigues B, Loehr J, Romero DA, Fremaux C, Horvath P, Doyon Y, Cambillau C, Moineau S. Nat Commun 9 2919 PMID:30046034
...All publications

Our group aims at understanding and analyzing the interactions of pathogens with their host, leading to its infection and its possible destruction. Our targets of interest are diverse, they gather i) bacteriophages infecting, among others, the lactic acid bacterium Lactococcus lactis used in the dairy industry, ii) bacterial secretion systems of type 6 and type 9, iii) the immune innate response of Drosophila after microbial or viral infection.

Understanding of host-pathogen interactions is at the heart of fighting pathogens involved in infectious diseases (bacteria, viruses). During the last ten years, we studied the structure and adhesion mechanism of bacteriophages (viruses that infect bacteria) infecting the bacterium used in the milk industry Lactococcus lactis. We have determined the structures of several phage virions by electron microscopy, and of the anti-receptor or the baseplate (1-2 MDa), eventually complexed to the receptor, by X-ray diffraction. We found that some phages, but not all, require an activation step by the calcium ion. More recently, we have addressed the study of type VI (T6SS) and Type IX (T9SS) secretion systems. We determined the structure and interactions of some of the 13 components of the T6SS, and we pursue the study of the system, including the trans-membrane domains. We wish to reconstruct the whole system by a "hybrid" approach, including electron microscopy and diffraction. The same approach is applied to T9SS. Finally, we have chosen the Drosophila model organism to study the innate immune response of the host. Genetic analysis has delineated two major regulatory pathways, the Toll and Imd pathways, that control the expression of anti microbial peptides (AMP) genes in response to microbial infection. We have solved the structure of recognition proteins (PGRP and GNBP) upstream of these two pathways.


Béatrice AMIGUES
UNK
Céline ARNAUD
Christian CAMBILLAU
UNK
Allele-Boubou COULIBALY
Hervé DARBON
Vincent DELAUZUN
Alexis DOGLIANI
Olivier FUCHSBAUER
Anaïs GAUBERT
Adeline GOULET
Magali GRANGE
Christine KELLENBERGER
Philippe LEONE
Alain ROUSSEL
UNK
Amal SALHI
Silvia SPINELLI
Thi Trang Nhung TRINH
UNK
Claire ZIMBERGER

© AFMB UMR7257  W3C validation