Team’s publications

Heads Bruno CANARD, Etienne DECROLY

2021

The enzymes for genome size increase and maintenance of large (+)RNA viruses (2021)

Ferron F, Sama B, Decroly E, Canard B .

Trends Biochem Sc in press PMID:34172362
System-oriented optimization of multi-target 2,6-diaminopurine derivatives: Easily accessible broad-spectrum antivirals active against flaviviruses, influenza virus and SARS-CoV-2 (2021)

Vicenti I, Martina MG, Boccuto A, De Angelis M, Giavarini G, Dragoni F, Marchi S, Trombetta C M, Crespan E, Maga G, Eydoux C, Decroly E, Montomoli E, Nencioni L, Zazzi M, Radi M.

Eur J Med Chem In press PMID:34273661
Structure and sequence requirements for RNA capping at the Venezuelan Equine Encephalitis Virus RNA 5'-end (2021)

Ortega Granda O, Valle C, Shannon A, Decroly E, Canard B, Coutard B, Rabah N.

J Virol In press PMID:34011549
Simeprevir potently suppresses SARS-CoV-2 replication and synergizes with remdesivir (2021)

Lo HS, Hui KPY, Lai HM, Khan KS, Kaur S, Huang J, Li JHZ, Chan AKN, Cheung HY, Ng KC, Ho JCW, Chen YW, Ma B, Cheung PMH, Shin D, Wang K, Lee MH, Selisko B, Eydoux C, Guillemot JC, Canard B, Wu KP, Liang PH, Dikic I, Zhong Zuo, Chan F K. L, Hui D S. C., Mok V C. T., Wong KB, Ko H, Shen Aik W, Chi Wai Chan M, Ng WL .

ACS Cent Sci 7 792-802 PMID:34075346
The WHO mission report struggles to trace the origins of the SARS-CoV-2 epidemic (tribune) (2021)

Decroly E, Claverie JM, Canard B.

Virologie (Montrouge) In press PMID:34112630
The methyltransferase domain of the Respiratory Syncytial Virus L protein catalyzes cap N7 and 2 O methylation (2021)

Sutto-Ortiz P, Tcherniuk S, Ysebaert N, Abeywickrema P, Noel M, Decombe A, Debart F, Vasseur JJ, Canard B, Roymans D, Rigaux P, Eleouet JF, Decroly E.

Plos Pathogens May 6 PMID:33956914
Dengue Virus NS5 Transcribes Metabolite-Capped, RIG-I Sensitive vRNAs (Abstract to the Biophysical Society 65th Annual Meeting) (2021)

Schweibenz B, Solotchi M, Decroly E, Selisko B, Canard B, Patel SS.

Biophysical J 120 (3) Suppl 1 135a PMID:0
[The WHO mission report struggles to trace the origins of the SARS-CoV-2 epidemic] (2021)

Decroly E, Claverie JM, Canard B.

Virologie (Montrouge) In press PMID:34018943
A high-throughput fluorescence polarization assay to discover inhibitors of arenavirus and coronavirus exoribonucleases (2021)

Hernandez S, Feracci M, Trajano De Jesus C, El-Kazzi P, Kaci R, Garlatti L, Decroly E, Canard B, Ferron F, Alvarez K.

bioRxiv 2021.04.02.437736 PMID:0
Furin cleaves SARS-CoV-2 spike-glycoprotein at S1/S2 and S2 prime for viral fusion/entry: indirect role of TMPRSS2 (2021)

Essalmani R, Jain J, Susan-Resiga D, Andreo U, Evagelidis A, Mouna Derbali R, Huynh DN, Dallaire F, Laporte M, Delpal A, Sutto-Ortiz P, Coutard B, Mapa C, Wilcoxen K, Decroly E, Pham TNQ, Cohen EA, Seidah NG .

bioRxiv 2020.12.18.423106 PMID:0
Fluoxetine targets an allosteric site in the enterovirus 2C AAA+ ATPase and stabilizes the hexameric complex (2021)

Hyrduss DL, El Kazzi P, Bauer L, Papageorgiou N, Ferron FP, Donselaar T, Van Vliet ALW, Canard B, Decroly E, Brancale A, Zeev Ben Mordehai T, Forster F, Van Kuppeveld FJM, Coutard B.

bioRxiv 2021.04.26.440876 PMID:0
Combining Antivirals and Immunomodulators to Fight COVID-19. (2021)

Feuillet V, Canard B, Trautmann A.

Trends Immunol 42 31-44 PMID:33281063
Structural biology in the fight against COVID-19. (2021)

Barcena M, Barnes CO, Beck M, Bjorkman PJ, Canard B, Gao GF, Gao Y, Hilgenfeld R, Hummer G, Patwardhan A, Santoni G, Saphire EO, Schaffitzel C, Schendel SL, Smith JL, Thorn A, Veesler D, Zhang P, Zhou Q.

Nat Struct Mol Biol 28 2-7 PMID:33437043
A fluorescence-based high throughput-screening assay for the SARS-CoV RNA synthesis complex (2021)

Eydoux C, Fattorini V, Shannon A, Le TT, Didier B, Canard B, Guillemot JC.

J Virol Methods 288 114013 PMID:33166547
Observation of arenavirus nucleoprotein heptamer assembly (2021)

Papageorgiou N, Vaitsopoulou A, Diop A, Nguyen THV, Canard B, Alvarez K, Ferron F.

FEBS Open Bio 11 1076-1083 PMID:33534950
AT-527, a Double Prodrug of a Guanosine Nucleotide Analog, Is a Potent Inhibitor of SARS-CoV-2 In Vitro and a Promising Oral Antiviral for Treatment of COVID-19 (2021)

Good SS, Westover J, Jung KH, Zhou XJ, Moussa A, La Colla P, Collu G, Canard B, Sommadossi JP.

Antimicrob Agents Chemother 18 2479-20 PMID:33558299
The nucleotide addition cycle of the SARS-CoV-2 polymerase (2021)

Bera SC, Seifert M, Kirchdoerfer RN, van Nies P, Wubulikasimu Y, Quack S, Papini FS, Arnold JJ, Canard B, Cameron CE, Depken M, Dulin D.

bioRxiv 2021.03.27.437309 PMID:33791706
Inhibition of SARS-CoV-2 polymerase by nucleotide analogs: a single molecule perspective (2021)

Seifert M, Bera SC, van Nies P, Kirchdoerfer RN, Shannon A, Le TT, Meng X, Xia H, Wood JM, Harris LD, Papini FS, Arnold JJ, Almo SC, Grove TL, Shi PY, Xiang Y, Canard B, Depken M, Cameron CE, Dulin D.

bioRxiv 2020.08.06.240325 PMID:33851161
Protein-primed RNA synthesis in SARS-CoVs and structural basis for inhibition by AT-527 (2021)

Shannon A, Fattorini V, Sama B, Selisko B, Feracci M, Falcou C, Gauffre P, El Kazzi P, Decroly E, Raba N, Alvarez K, Eydoux C, Guillemot JC, Debart F, Vasseur JJ, Noel M, Moussa A, Good S, Lin K, Sommadossi JP, Zhu Y, Yan X, Shi H, Ferron F, Canard B .

bioRxiv 2021.03.23.436564 PMID:0
Observation of arenavirus nucleoprotein heptamer assembly (2021)

Papageorgiou N, Vaitsopoulou A, Diop A, Nguyen THV, Canard B, Alvarez K, Ferron F.

FEBS Open Bio 11 1076-1083 PMID:33534950
Tracing the origins of SARS-COV-2 in coronavirus phylogenies: a review. (2021)

Sallard E, Halloy J, Casane D, Decroly E, van Helden J.

Environ Chem Lett. Feb 4: 1-17 PMID:33558807
COVID-19 epidemiologic surveillance using wastewater. (2021)

Sharma VK, Jinadatha C, Lichtfouse E, Decroly E, van Helden J, Choi H, Chatterjee P.

Environ Chem Lett. Jan 28 1-5 PMID:33531884
First insights into the structural features of Ebola virus methyltransferase activities (2021)

Valle C, Martin B, Ferron F, Roig-Zamboni V, Desmyter A, Debart F, Vasseur JJ, Canard B, Coutard B, Decroly E.

Nucleic Acids Res 49 1737-1748 PMID:33503246

2020

Synthesis and biological evaluation of novel flexible nucleoside analogues that inhibit flavivirus replication in vitro (2020)

Thames JE, Waters CD 3rd, Valle C, Bassetto M, Aouadi W, Martin B, Selisko B, Falat A, Coutard B, Brancale A, Canard B, Decroly E, Seley-Radtke KL.

Bioorg Med Chem 28 115713 PMID:33128910
Rapid incorporation of Favipiravir by the fast and permissive viral RNA polymerase complex results in SARS-CoV-2 lethal mutagenesis (2020)

Shannon A, Selisko B, Le NT, Huchting J, Touret F, Piorkowski G, Fattorini V, Ferron F, Decroly E, Meier C, Coutard B, Peersen O, Canard B.

Nat Commun 11 4682 PMID:32943628
Mutations on VEEV nsP1 relate RNA capping efficiency to ribavirin susceptibility (2020)

Rabah N, Ortega Granda O, Querat G, Canard B, Decroly E, Coutard B.

Antiviral Res 182 104883 PMID:32750467
Antiviral activity of the natural alkaloid anisomycin against dengue and Zika viruses (2020)

Quintana VM, Selisko B, Brunetti JE, Eydoux C, Guillemot JC, Canard B, Damonte EB, Julander JG, Castilla V.

Antiviral Res 176 104749 PMID:32081740
Brothers in Arms: Structure, Assembly and Function of Arenaviridae Nucleoprotein (2020)

Papageorgiou N, Spiliopoulou M, Van Nguyen TH, Vaitsopoulou A, Laban EY, Alvarez K, Margiolaki I, Canard B, Ferron F.

Viruses 12 772 PMID:32708976
Snapshots of ADP-ribose bound to Getah virus macro domain reveal an intriguing choreography (2020)

Ferreira-Ramos AS, Sulzenbacher G, Canard B, Coutard B.

Sci Rep 10 14422 PMID:32879358
Synthesis of Adenine Dinucleosides SAM Analogs as Specific Inhibitors of SARS-CoV nsp14 RNA Cap guanine-N7-methyltransferase (2020)

Ahmed-Belkacem R, Sutto-Ortiz P, Guiraud M, Canard B, Vasseur JJ, Decroly E, Debart F..

Eur J Med Chem 112557 PMID:32563813
Retrouver les origines du SARS-COV-2 dans les phylogenies de coronavirus (2020)

Sallard E, Halloy H, Casane D, van Helden J., Decroly E.

Med Sci (Paris) 36 783-796 PMID:32773024
Favipiravir strikes the SARS-CoV-2 at its Achilles heel, the RNA polymerase (2020)

Shannon A, Selisko B, Le N, Huchting J, Touret F, Piorkowski G, Fattorini V, Ferron F, Decroly E, Meier C, Coutard B, Peersen O, Canard B.

bioRxiv 2020.05.15.098731 PMID:32511380
Drugs against SARS-CoV-2: what do we know about their mode of action? (2020)

Valle C, Martin B, Touret F, Shannon A, Canard B., Guillemot JC, Coutard B Decroly E.

Rev Med Virol 30 1-10 PMID:32779326
In vitro screening of a FDA approved chemical library reveals potential inhibitors of SARS-CoV-2 replication (2020)

Touret F, Gilles M, Barral K, Nougairede A, van Helden J, Decroly E, de Lamballerie X, Coutard B.

Sci Rep 10 13093 PMID:32753646
The C-terminal domain of the Sudan ebolavirus L protein is essential for RNA binding and methylation. (2020)

Valle C, Martin B, Debart F, Vasseur JJ, Imbert I, Canard B, Coutard B, Decroly E.

J Virol 94 e00520-20 PMID:32269120
Remdesivir and SARS-CoV-2: structural requirements at both nsp12 RdRp and nsp14 Exonuclease active-sites. (2020)

Shannon A, Tuyet Le NT, Selisko B, Eydoux C, Alvarez K, Guillemot JC, Decroly E, Peersen O, Ferron F, Canard B.

Antiviral Res 178 104793 PMID:32283108
A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae. (2020)

Ferron F, Debat HJ, Shannon A, Decroly E, Canard B.

NAR Genom Bioinform 2 lqz022 PMID:32289120
The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade. (2020)

Coutard B, Valle C, de Lamballerie X, Canard B, Seidah NG, Decroly E.

Antiviral Res 176 104742 PMID:32057769
Design, synthesis and discovery of novel N,N'-carbazoyl-aryl-urea inhibitors of Zika NS5 methyltransferase and virus replication. (2020)

Spizzichino S, Mattedi G, Lauder K, Valle C, Aouadi W, Canard B, Decroly E, Kaptein SJF, Neyts J, Graham C, Sule Z, Barlow DJ, Silvestri R, Castagnolo D.

ChemMedChem 15 385-390 PMID:31805205

2019

Deciphering the Nucleotide and RNA Binding Selectivity of the Mayaro Virus Macro Domain (2019)

Tsika AC, Melekis E, Tsatsouli SA, Papageorgiou N, Mate MJ, Canard B, Coutard B, Bentrop D, Spyroulias GA.

J Mol Biol 431 2283-2297 PMID:30998933
Conformational plasticity of the VEEV macro domain is important for binding of ADP-ribose (2019)

Makrynitsa GI, Ntonti D, Marousis KD, Birkou M, Matsoukas MT, Asami S, Bentrop D, Papageorgiou N, Canard B, Coutard B, Spyroulias GA.

J Struct Biol 206 119-127 PMID:30825649
Fluoxetine inhibits enterovirus replication by targeting the viral 2C protein in a stereospecific manner (2019)

Bauer L, Manganaro R, Zonsics B, Strating JRPM, El Kazzi P, Lorenzo Lopez M, Ulferts R, van Hoey C, Mate MJ, Langer T, Coutard B, Brancale A, van Kuppeveld FJM.

ACS Infect Dis 5 1609-1623 PMID:31305993
Structure of the Respiratory Syncytial Virus Polymerase Complex. (2019)

Gilman MSA, Liu C, Fung A, Behera I, Jordan P, Rigaux P, Ysebaert N, Tcherniuk S, Sourimant J, Eleouet JF, Sutto-Ortiz P, Decroly E, Roymans D, Jin Z, McLellan JS.

Cell 179 193-204 PMID:31495574
The Curious Case of the Nidovirus Exoribonuclease: Its Role in RNA Synthesis and Replication Fidelity. (2019)

Ogando NS, Ferron F, Decroly E, Canard B, Posthuma CC, Snijder EJ.

Front Microbiol 10 1813 PMID:31440227
Structure and oligomerization state of the C-terminal region of the Middle East respiratory syndrome coronavirus nucleoprotein. (2019)

Nguyen THV, Lichiere J, Canard B, Papageorgiou N, Attoumani S, Ferron F, Coutard B.

Acta Crystallogr D Struct Biol 75 8-15 PMID:30644840
Metal chelators for the inhibition of the lymphocytic choriomeningitis virus endonuclease domain (2019)

Saez-Ayala M, Laban Yekwa E, Mondielli C, Roux L, Hernandez S, Bailly F, Cotelle P, Rogolino D, Canard B, Ferron F, Alvarez K.

Antiviral Res. 162 79-89 PMID:30557576
Identification of a new natural gastric lipase inhibitor from star anise. (2019)

Kamoun J, Rahier R, Sellami M, Koubaa I, Mansuelle P, Lebrun R, Berlioz-Barbier A, Fiore M, Alvarez K, Abousalham A, Carriere F, Aloulou A.

Food Funct 10 469-478 PMID:30632597
C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system. (2019)

Jais PH, Decroly E, Jacquet E, Le Boulch M, Jais A, Jean-Jean O, Eaton H, Ponien P, Verdier F, Canard B, Goncalves S, Chiron S, Le Gall M, Mayeux P, Shmulevitz M.

Nucleic Acids Res 47 2681-2698 PMID:30726994
Approved drugs screening against the nsP1 capping enzyme of Venezuelan equine encephalitis virus using an immuno-based assay. (2019)

Ferreira-Ramos AS, Li C, Eydoux C, Contreras JM, Morice C, Querat G, Gigante A, Perez Perez MJ, Jung ML, Canard B, Guillemot JC, Decroly E, Coutard B.

Antiviral Res 163 59-69 PMID:30639438
FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing. (2019)

Ringeard M, Marchand V, Decroly E, Motorin Y, Bennasser Y.

Nature 565 500-504 PMID:30626973

2018

[Ebola virus L protein harbors a new enzymatic activity involved in the internal methylation of RNAs]. (2018)

Martin B, Valle C, Coutard B, Canard B, Debart F, Decroly E.

Med Sci (Paris) 34 919-921 PMID:30526829
Optimization of a fragment linking hit toward Dengue and Zika virus NS5 methyltransferases inhibitors. (2018)

Hernandez J, Hoffer L, Coutard B, Querat G, Roche P, Morelli X, Decroly E, Barral K.

Eur J Med Chem 161 323-333 PMID:30368131
[Innate immunity evasion mechanisms of filoviruses]. (2018)

Martin B, Decroly E.

Med Sci (Paris) 34 671-677 PMID:30230452
Crystal structures of Lymphocytic choriomeningitis virus endonuclease domain complexed with diketo-acid ligands. (2018)

Saez-Ayala M, Yekwa EL, Carcelli M, Canard B, Alvarez K, Ferron F.

IUCrJ 5 223-235 PMID:29765612
Structural and Functional Basis of the Fidelity of Nucleotide Selection by Flavivirus RNA-Dependent RNA Polymerases. (2018)

Selisko B, Papageorgiou N, Ferron F, Canard B.

Viruses 10 59 PMID:29385764
How to Control HTLV-1-Associated Diseases: Preventing de Novo Cellular Infection Using Antiviral Therapy. (2018)

Pasquier A, Alais S, Roux L, Thoulouze MI, Alvarez K, Journo C, Dutartre H, Mahieux R.

Front Microbiol 9 278 PMID:29593659
Synthesis and substrate properties towards HIV-1 reverse transcriptase of new diphosphate analogues of 9-[(2-phosphonomethoxy)ethyl]adenine. (2018)

Laux WH, Priet S, Alvarez K, Peyrottes S, Perigaud C.

Antivir Chem Chemother 26 2040206618757636 PMID:29436843
The European Virus Archive goes global: A growing resource for research. (2018)

Romette JL, Prat CM, Gould EA, de Lamballerie X, Charrel R, Coutard B, Fooks AR, Bardsley M, Carroll M, Drosten C, Drexler JF, Gunther S, Klempa B, Pinschewer D, Klimkait T, Avsic-Zupanc T, Capobianchi MR, Dicaro A, Ippolito G, Nitsche A, Koopmans M, Reusken C, Gorbalenya A, Raoul H, Bourhy H, Mettenleiter T, Reiche S, Batten C, Sabeta C, Paweska JT, Eropkin M, Zverev V, Hu Z, Mac Cullough S, Mirazimi A, Pradel F, Lieutaud P.

Antiviral Res 158 127-134 PMID:30059721
The methyltransferase domain of the Sudan ebolavirus L protein specifically targets internal adenosines of RNA substrates, in addition to the cap structure. (2018)

Martin B, Coutard B, Guez T, Paesen GC, Canard B, Debart F, Vasseur JJ, Grimes JM, Decroly E.

Nucleic Acids Res 46 7902-7912 PMID:30192980
Hijacking DNA methyltransferase transition state analogues to produce chemical scaffolds for PRMT inhibitors. (2018)

Halby L, Marechal N, Pechalrieu D, Cura V, Franchini DM, Faux C, Alby F, Troffer-Charlier N, Kudithipudi S, Jeltsch A, Aouadi W, Decroly E, Guillemot JC, Page P, Ferroud C, Bonnefond L, Guianvarc'h D, Cavarelli J, Arimondo PB.

Philos Trans R Soc Lond B Biol Sci 373 PMID:29685976
Filovirus proteins for antiviral drug discovery: Structure/function of proteins involved in assembly and budding. (2018)

Martin B, Reynard O, Volchkov V, Decroly E.

Antiviral Res 150 183-192 PMID:29305306
Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA. (2018)

Ferron F, Subissi L, Silveira De Morais AT, Le NTT, Sevajol M, Gluais L, Decroly E, Vonrhein C, Bricogne G, Canard B, Imbert I.

Proc Natl Acad Sci U S A 115 E162-E171 PMID:29279395

2017

The structure of the binary methyltransferase-SAH complex from Zika virus reveals a novel conformation for the mechanism of mRNA capping (2017)

Chatrin C, Talapatra SK, Canard B, Kozielski F.

Oncotarget 9 3160-3171 PMID:29423037
Toscana virus nucleoprotein oligomer organization observed in solution. (2017)

Baklouti A, Goulet A, Lichiere J, Canard B, Charrel RN, Ferron F, Coutard B, Papageorgiou N.

Acta Crystallogr D Struct Biol 73 650-659 PMID:28777080
Toward the identification of viral cap-methyltransferase inhibitors by fluorescence screening assay. (2017)

Aouadi W, Eydoux C, Coutard B, Martin B, Debart F, Vasseur JJ, Contreras JM, Morice C, Querat G, Jung ML, Canard B, Guillemot JC, Decroly E.

Antiviral Res 144 330-339 PMID:28676301
Antiviral activity of [1,2,3]triazolo[4,5-d]pyrimidin-7(6H)-ones against chikungunya virus targeting the viral capping nsP1. (2017)

Gigante A, Gomez-SanJuan A, Delang L, Li C, Bueno O, Gamo AM, Priego EM, Camarasa MJ, Jochmans D, Leyssen P, Decroly E, Coutard B, Querat G, Neyts J, Perez-Perez MJ.

Antiviral Res. 144 216-222 PMID:28619679
Biochemical principles and inhibitors to interfere with viral capping pathways. (2017)

Decroly E, Canard B.

Curr Opin Virol 24 87-96 PMID:28527860
Substrate selectivity of Dengue and Zika virus NS5 polymerase towards 2'-modified nucleotide analogues. (2017)

Potisopon S, Ferron F, Fattorini V, Selisko B, Canard B.

Antiviral Res 140 25-36 PMID:28041959
Filovirus proteins for antiviral drug discovery: Structure/function bases of the replication cycle. (2017)

Martin B, Canard B, Decroly E.

Antiviral Res 141 48-61 PMID:28192094
Binding of the methyl donor SAM to MERS-CoV 2' -O-methyltransferase nsp16 promotes the recruitment of the allosteric activator nsp10. (2017)

Aouadi W, Blanjoie A, Vasseur JJ, Debart F, Canard B, Decroly E.

J Virol 91 e02217-16 PMID:28031370
The Zika virus methyltransferase: structure and functions for drug design perspectives. (2017)

Coutard B, Barral K, Lichiere J, Selisko B, Martin B, Aouadi W, Ortiz Lombardia M, Debart F, Vasseur JJ, Guillemot JC, Canard B, Decroly E.

J Virol 91 e02202-16 PMID:28031359

2016

Contribution to PNA-RNA chimera synthesis: one pot microwave-assisted Ugi reaction to obtain dimeric building blocks (2016)

Ovadia R, Vasseur JJ, Baraguey C, Alvarez K.

Eur J Org Chem PMID:0
Coxsackievirus B3 protease 3C: expression, purification, crystallization and preliminary structural insights. (2016)

Fili S, Valmas A, Christopoulou M, Spiliopoulou M, Nikolopoulos N, Lichiere J, logotheti S, Karavassili F, Rosmaraki E, Fitch A, Wright A, Beckers D, Degen T, Nenert G, Hilgenfeld R, Papageorgiou N, Canard B, Coutard B, Margiolaki I.

Acta Crystallogr F Struct Biol Commun 72 877-884 PMID:27917835
Involvement of an Arginine Triplet in M1 Matrix Protein Interaction with Membranes and in M1 Recruitment into Virus-Like Particles of the Influenza A(H1N1)pdm09 Virus. (2016)

Kerviel A, Dash S, Moncorge O, Panthu B, Prchal J, Decimo D, Ohlmann T, Lina B, Favard C, Decroly E, Ottmann M, Roingeard P, Muriaux D.

PLoS One 11 e0165421 PMID:27814373
Discovery of novel dengue virus NS5 methyltransferase non-nucleoside inhibitors by fragment-based drug design. (2016)

Benmansour F, Trist I, Coutard B, Decroly E, Querat G, Brancale A, Barral K.

Eur J Med Chem 125 865-880 PMID:27750202
Filovirus proteins for antiviral drug discovery: A structure/function analysis of surface glycoproteins and virus entry. (2016)

Martin B, Hoenen T, Canard B, Decroly E.

Antiviral Res 135 1-14 PMID:27640102
The methyltransferase domain of dengue virus protein NS5 ensures efficient RNA synthesis initiation and elongation by the polymerase domain. (2016)

Potisopon S, Priet S, Collet A, Decroly E, Canard B, Selisko B.

Nucleic Acids Res 44 2974 PMID:26578566
Reevaluation of possible outcomes of infections with human immunodeficiency virus. (2016)

Tamalet C, Colson P, Decroly E, Dhiver C, Ravaux I, Stein A, Raoult D.

Clin Microbiol Infect 22 299-311 PMID:26794031
The Nonstructural Proteins Directing Coronavirus RNA Synthesis and Processing (2016)

Snijder E J, Decroly E, Ziebuhr J.

Adv Virus Res 96 59-126 PMID:27712628
The viral capping enzyme nsP1: a novel target for the inhibition of chikungunya virus infection. (2016)

Delang L, Li C, Tas A, Querat G, Albulescu IC, De Burghgraeve T, Guerrero NA, Gigante A, Piorkowski G, Decroly E, Jochmans D, Canard B, Snijder EJ, Perez-Perez MJ, van Hemert MJ, Coutard B, Leyssen P, Neyts J.

Sci Rep 6 31819 PMID:27545976
Viral Macro Domains Reverse Protein ADP-ribosylation. (2016)

Li C, Debing Y, Jankevicius G, Neyts J, Ahel I, Coutard B, Canard B.

J Virol 90 8478-86 PMID:27440879
Complete Coding Sequences of Six Toscana Virus Strains Isolated from Human Patients in France. (2016)

Baklouti A, Leparc-Goffart I, Piorkowski G, Coutard B, Papageorgiou N, De Lamballerie X, Charrel RN..

Genome Announc. 4 e00454-16 PMID:27231377
Structural characterization of the N-terminal part of the MERS-CoV nucleocapsid by X-ray diffraction and small-angle X-ray scattering. (2016)

Papageorgiou N, Lichiere J, Baklouti A, Ferron F, Sevajol M, Canard B, Coutard B.

Acta Crystallogr D Struct Biol 72 192-202 PMID:26894667
Complete Coding Sequences of Three Toscana Virus Strains Isolated from Sandflies in France. (2016)

Baklouti A, Leparc Goffard I, Piorkowski G, Coutard B, Papageorgiou N, De Lamballerie X, Charrel RN..

Genome Announc. 4 e01676-15 PMID:26868387
Screening of a library of FDA-approved drugs identifies several enterovirus replicaton inhibitors that target viral protein 2C. (2016)

Ulferts R, de Boer M, van der Linden L, Bauer L, Lyoo HR, Mate MJ, Lichiere J, Canard B, Lelieveld D, Omta W, Egan D, Coutard B, van Kuppeveld FJ.

Antimicrob Agents Chemother 60 2627-38 PMID:26856848
Novel 2-phenyl-5-[(E)-2-(thiophen-2-yl)ethenyl]-1,3,4-oxadiazole and 3-phenyl-5-[(E)-2-(thiophen-2-yl)ethenyl]-1,2,4-oxadiazole derivatives as dengue virus inhibitors targeting NS5 polymerase. (2016)

Benmansour F, Eydoux C, Querat G, de Lamballerie X, Canard B, Alvarez K, Guillemot JC, Barral K.

Eur J Med Chem 109 146-56 PMID:26774922

2015

Arenavirus Glycan Shield Promotes Neutralizing Antibody Evasion and Protracted Infection. (2015)

Sommerstein R, Flatz L, Remy MM, Malinge P, Magistrelli G, Fischer N, Sahin M, Bergthaler A, Igonet S, Ter Meulen J, Rigo D, Meda P, Rabah N, Coutard B, Bowden TA, Lambert PH, Siegrist CA, Pinschewer DD.

PLoS Pathog 11 e1005276 PMID:26587982
Characterisation of Structural Proteins from Chronic Bee Paralysis Virus (CBPV) Using Mass Spectrometry. (2015)

Chevin A, Coutard B, Blanchard P, Dabert-Gay AS, Ribiere-Chabert M, Thiery R.

Viruses 7 3329-44 PMID:26110588
The RNA template channel of the RNA-dependent RNA polymerase as a target for development of antiviral therapy of multiple genera within a virus family. (2015)

van der Linden L, Vives-Adrian L, Selisko B, Ferrer-Orta C, Liu X, Lanke K, Ulferts R, De Palma AM, Tanchis F, Goris N, Lefebvre D, De Clercq K, Leyssen P, Lacroix C, Purstinger G, Coutard B, Canard B, Boehr DD, Arnold JJ, Cameron CE, Verdaguer N, Neyts J, van Kuppeveld FJ.

PLoS Pathog 11 e1004733 PMID:25799064
X-ray structure and activities of an essential Mononegavirales L-protein domain (2015)

Paesen G C, Collet A, Sallamand C, Debart F, Vasseur JJ, Canard B, Decroly E, Grimes J M.

Nat Commun 6 8749 PMID:26549102
Enzymatic synthesis of acyclic nucleoside thiophosphonate diphosphates: effect of the alpha-phosphorus configuration on HIV-1 RT activity. (2015)

Priet S, Roux L, Saez-Ayala M, Ferron F, Canard B, Alvarez K.

Antiviral Res 117 122-31 PMID:25766862
mRNA capping by Venezuelan Equine Encephalitis Virus nsP1: Functional characterization and implication for antiviral research. (2015)

Li C, Guillen J, Rabah N, Blanjoie A, Debart F, Vasseur JJ, Canard B, Decroly E, Coutard B.

J Virol 89 8292-303 PMID:26041283
Structural and biophysical analysis of sequence insertions in the Venezuelan Equine Encephalitis Virus macro domain. (2015)

Guillen J, Lichiere J, Rabah N, Beitzel BF, Canard B, Coutard B.

Virus Res 201 94-100 PMID:25725151
The Megavirus chilensis Cu,Zn-superoxide dismutase: the first viral structure of a typical CCS-independent hyperstable dimeric enzyme. (2015)

Lartigue A, Burlat B, Coutard B, Chaspoul F, Claverie JM, Abergel C.

J Virol 89 824-832 PMID:25355875
NMR study of non-structural proteins-part II: H, C, N backbone and side-chain resonance assignment of macro domain from Venezuelan equine encephalitis virus (VEEV). (2015)

Makrynitsa GI, Ntonti D, Marousis KD, Tsika AC, Lichiere J, Papageorgiou N, Coutard B, Bentrop D, Spyroulias GA.

Biomol NMR Assign 9 247-51 PMID:25291978
NMR study of non-structural proteins-part I: H, C, N backbone and side-chain resonance assignment of macro domain from Mayaro virus (MAYV). (2015)

Melekis E, Tsika AC, Lichiere J, Chasapis CT, Margiolaki I, Papageorgiou N, Coutard B, Bentrop D, Spyroulias GA.

Biomol NMR Assign 9 191-5 PMID:25217003
The methyltransferase domain of dengue virus protein NS5 ensures efficient RNA synthesis initiation and elongation by the polymerase domain. (2015)

Potisopon S, Priet S, Collet A, Decroly E, Canard B, Selisko B.

Nucleic Acids Res 42 11642-11656 PMID:25209234

2014

(R)-9-[2-(Hydroxyphosphinylmethoxy)propyl]adenine as the precursor molecule for antivirals (2014)

L. Roux, B. Canard, K. Alvarez.

Tet. Lett. 4168-4171 PMID:0
Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes. (2014)

Bouvet M, Lugari A, Posthuma CC, Zevenhoven JC, Bernard S, Betzi S, Imbert I, Canard B, Guillemot JC, Lecine P, Pfefferle S, Drosten C, Snijder EJ, Decroly E, Morelli X.

J Biol Chem 289 25783-96 PMID:25074927
One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities. (2014)

Subissi L, Posthuma CC, Collet A, Zevenhoven-Dobbe JC, Gorbalenya AE, Decroly E, Snijder EJ, Canard B, Imbert I.

Proc Natl Acad Sci U S A 111 E3900-9 PMID:25197083
Insights into RNA synthesis, capping, and proofreading mechanisms of SARS-coronavirus. (2014)

Sevajol M, Subissi L, Decroly E, Canard B, Imbert I.

Virus Res 194 90-99 PMID:25451065
Coiled-coil deformations in crystal structures: the measles virus phosphoprotein multimerization domain as an illustrative example. (2014)

Blocquel D, Habchi J, Durand E, Sevajol M, Ferron F, Erales J, Papageorgiou N, Longhi S.

Acta Crystallogr D Biol Crystallogr 70 1589-1603 PMID:24914970
The Crystal Structure of a Cardiovirus RNA-Dependent RNA Polymerase Reveals an Unusual Conformation of the Polymerase Active Site. (2014)

Vives-Adrian L, Lujan C, Oliva B, van der Linden L, Selisko B, Coutard B, Canard B, van Kuppeveld FJ, Ferrer-Orta C, Verdaguer N.

J Virol 88 5595-607 PMID:24600002
Assessment of Dengue virus helicase and methyltransferase as targets for fragment-based drug discovery. (2014)

Coutard B, Decroly E, Li C, Sharff A, Lescar J, Bricogne G, Barral K.

Antiviral Res 106C 61-70 PMID:24704437
SARS-CoV ORF1b-encoded nonstructural proteins 12-16: replicative enzymes as antiviral targets. (2014)

Subissi L, Imbert I, Ferron F, Collet A, Coutard B, Decroly E, Canard B.

Antiviral Res 101 122-30 PMID:24269475

2013

Improving the soluble expression of recombinant proteins by randomly shuffling 5' and 3' coding-sequence ends. (2013)

Bignon C, Li C, Lichiere J, Canard B, Coutard B.

Acta Crystallogr D Biol Crystallogr 69 2580-2 PMID:24311598
Structure-Activity Relationship Study of Biflavonoids on the Dengue Virus Polymerase DENV-NS5 RdRp. (2013)

Coulerie P, Nour M, Maciuk A, Eydoux C, Guillemot JC, Lebouvier N, Hnawia E, Leblanc K, Lewin G, Canard B, Figadere B.

Planta Med 79 1313-8 PMID:23929244
Development of specific dengue virus 2'-O- and N7-methyltransferase assays for antiviral drug screening. (2013)

Barral K, Sallamand C, Petzold C, Coutard B, Collet A, Thillier Y, Zimmermann J, Vasseur JJ, Canard B, Rohayem J, Debart F, Decroly E.

Antiviral Res 99 292-300 PMID:23769894
SAMHD1 restricts the replication of human immunodeficiency virus type 1 by depleting the intracellular pool of deoxynucleoside triphosphates. (2013)

Lahouassa H, Daddacha W, Hofmann H, Ayinde D, Logue EC, Dragin L, Bloch N, Maudet C, Bertrand M, Gramberg T, Pancino G, Priet S, Canard B, Laguette N, Benkirane M, Transy C, Landau NR, Kim B, Margottin-Goguet F.

Nat Immunol 14 877 PMID:23867943
3',5'Di-O-trityluridine inhibits in vitro flavivirus replication. (2013)

De Burghgraeve T, Selisko B, Kaptein S, Chatelain G, Leyssen P, Debing Y, Jacobs M, Van Aerschot A, Canard B, Neyts J.

Antiviral Res 98 242-7 PMID:23470860
Nuclear localization of dengue virus nonstructural protein 5 does not strictly correlate with efficient viral RNA replication and inhibition of type I interferon signaling. (2013)

Kumar A, Buhler S, Selisko B, Davidson A, Mulder K, Canard B, Miller S, Bartenschlager R.

J Virol 87 4545-57 PMID:23408610
Selective Serotonin Reuptake Inhibitor Fluoxetine Inhibits Replication of Human Enteroviruses B and D by Targeting Viral Protein 2C. (2013)

Ulferts R, van der Linden L, Thibaut HJ, Lanke KH, Leyssen P, Coutard B, De Palma AM, Canard B, Neyts J, van Kuppeveld FJ.

Antimicrob Agents Chemother 57 1952-6 PMID:23335743

2012

Biflavonoids of Dacrydium balansae with Potent Inhibitory Activity on Dengue 2 NS5 Polymerase. (2012)

Coulerie P, Eydoux C, Hnawia E, Stuhl L, Maciuk A, Lebouvier N, Canard B, Figadere B, Guillemot JC, Nour M.

Planta Med 78 672-7 PMID:22411725
Chemical constituents of Anacolosa pervilleana and their antiviral activities. (2012)

Bourjot M, Leyssen P, Eydoux C, Guillemot JC, Canard B, Rasoanaivo P, Gueritte F, Litaudon M.

Fitoterapia 83 1076-80 PMID:22613073
RNA 3'-end mismatch excision by the severe acute respiratory syndrome coronavirus nonstructural protein nsp10 nsp14 exoribonuclease complex. (2012)

Bouvet M, Imbert I, Subissi L, Gluais L, Canard B, Decroly E.

Proc Natl Acad Sci U S A 109 9372-7 PMID:22635272
The viral RNA capping machinery as a target for antiviral drugs. (2012)

Ferron F, Decroly E, Selisko B, Canard B.

Antiviral Res 96 21-31 PMID:22841701
Antiviral chlorinated daphnane diterpenoid orthoesters from the bark and wood of Trigonostemon cherrieri. (2012)

Allard PM, Leyssen P, Martin MT, Bourjot M, Dumontet V, Eydoux C, Guillemot JC, Canard B, Poullain C, Gueritte F, Litaudon M.

Phytochemistry 84 160-8 PMID:22938995
Molecular basis for nucleotide conservation at the ends of the dengue virus genome. (2012)

Selisko B, Potisopon S, Agred R, Priet S, Varlet I, Thillier Y, Sallamand C, Debart F, Vasseur JJ, Canard B.

PLoS Pathog 8 e1002912 PMID:23028313
[Capping strategies in RNA viruses.] (2012)

Bouvet M, Ferron F, Imbert I, Gluais L, Selisko B, Coutard B, Canard B, Decroly E.

Med Sci (Paris) 28 423-429 PMID:22549871
Flacourtosides A-F, Phenolic Glycosides Isolated from Flacourtia ramontchi. (2012)

Bourjot M, Leyssen P, Eydoux C, Guillemot JC, Canard B, Rasoanaivo P, Gueritte F, Litaudon M.

J Nat Prod 75 752-8 PMID:22439591
Synthesis of 5' cap-0 and cap-1 RNAs using solid-phase chemistry coupled with enzymatic methylation by human (guanine-N7)-methyl transferase. (2012)

Thillier Y, Decroly E, Morvan F, Canard B, Vasseur JJ, Debart F.

RNA 18 856-68 PMID:22334760
Single pH buffer refolding screen for protein from inclusion bodies. (2012)

Coutard B, Danchin EG, Oubelaid R, Canard B, Bignon C.

Protein Expr Purif 82 352-359 PMID:22343064
SAMHD1 restricts the replication of human immunodeficiency virus type 1 by depleting the intracellular pool of deoxynucleoside triphosphates. (2012)

Lahouassa H, Daddacha W, Hofmann H, Ayinde D, Logue EC, Dragin L, Bloch N, Maudet C, Bertrand M, Gramberg T, Pancino G, Priet S, Canard B, Laguette N, Benkirane M, Transy C, Landau NR, Kim B, Margottin-Goguet F.

Nat Immunol 13 223-8 PMID:22327569

2011

In vitro antiviral activities of extracts derived from Daucus maritimus seeds. (2011)

Miladi S, Abid N, Debarnot C, Damak M, Canard B, Aouni M, Selmi B.

Nat Prod Res 26 1027-32 PMID:21895456
Conventional and unconventional mechanisms for capping viral mRNA. (2011)

Decroly E, Ferron F, Lescar J, Canard B.

Nat Rev Microbiol 10 51-65 PMID:22138959
Alkylated Flavanones from the Bark of Cryptocarya chartacea As Dengue Virus NS5 Polymerase Inhibitors. (2011)

Allard PM, Dau ET, Eydoux C, Guillemot JC, Dumontet V, Poullain C, Canard B, Gueritte F, Litaudon M.

J Nat Prod 74 2446-53 PMID:22050318
Flavivirus NS3 and NS5 proteins interaction network: A high-throughput yeast two-hybrid screen. (2011)

Le Breton M, Meyniel-Schicklin L, Deloire A, Coutard B, Canard B, de Lamballerie X, Andre P, Rabourdin-Combe C, Lotteau V, Davoust N.

BMC Microbiol 11 234 PMID:22014111
Structural basis for antiviral inhibition of the main protease 3C from human enterovirus 93. (2011)

Costenaro L, Kaczmarska Z, Arnan C, Janowski R, Coutard B, Sola M, Gorbalenya AE, Norder H, Canard B, Coll M.

J Virol 85 10764-73 PMID:21835784
Acyclic nucleoside thiophosphonates as potent inhibitors of HIV and HBV replication. (2011)

Barral K, Weck C, Payrot N, Roux L, Durafour C, Zoulim F, Neyts J, Balzarini J, Canard B, Priet S, Alvarez K.

Eur J Med Chem 46 4281-8 PMID:21803462
Arenaviruses and hantaviruses: from epidemiology and genomics to antivirals. (2011)

Charrel RN, Coutard B, Baronti C, Canard B, Nougairede A, Frangeul A, Morin B, Jamal S, Schmidt CL, Hilgenfeld R, Klempa B, de Lamballerie X.

Antiviral Res 90 102-14 PMID:21356244
Comparative production analysis of three phlebovirus nucleoproteins under denaturing or non-denaturing conditions for crystallographic studies. (2011)

Lantez V, Dalle K, Charrel R, Baronti C, Canard B, Coutard B.

PLoS Negl Trop Dis 5 e936 PMID:21245924
The hexamer structure of the rift valley Fever virus nucleoprotein suggests a mechanism for its assembly into ribonucleoprotein complexes. (2011)

Ferron F, Li Z, Danek EI, Luo D, Wong Y, Coutard B, Lantez V, Charrel R, Canard B, Walz T, Lescar J.

PLoS Pathog 7 e1002030 PMID:21589902
Crystal Structure and Functional Analysis of the SARS-Coronavirus RNA Cap 2'-O-Methyltransferase nsp10/nsp16 Complex. (2011)

Decroly E, Debarnot C, Ferron F, Bouvet M, Coutard B, Imbert I, Gluais L, Papageorgiou N, Sharff A, Bricogne G, Ortiz-Lombardia M, Lescar J, Canard B.

PLoS Pathog 7 e1002059 PMID:21637813
Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex. (2011)

Debarnot C, Imbert I, Ferron F, Gluais L, Varlet I, Papageorgiou N, Bouvet M, Lescar J, Decroly E, Canard B.

Acta Crystallogr Sect F Struct Biol Cryst Commun 67 404-8 PMID:21393853
Picornavirus non-structural proteins as targets for new anti-virals with broad activity. (2011)

Norder H, De Palma AM, Selisko B, Costenaro L, Papageorgiou N, Arnan C, Coutard B, Lantez V, De Lamballerie X, Baronti C, Sola M, Tan J, Neyts J, Canard B, Coll M, Gorbalenya AE, Hilgenfeld R.

Antiviral Res 89(3) 204-218 PMID:21236302

2010

Molecular mapping of the RNA Cap 2'-O-methyltransferase activation interface between severe acute respiratory syndrome coronavirus nsp10 and nsp16. (2010)

Lugari A, Betzi S, Decroly E, Bonnaud E, Hermant A, Guillemot JC, Debarnot C, Borg JP, Bouvet M, Canard B, Morelli X, Lecine P.

J Biol Chem 285 33230-41 PMID:20699222
3'-Deoxy phosphoramidate dinucleosides as improved inhibitors of hepatitis C virus subgenomic replicon and NS5B polymerase activity. (2010)

Priet S, Zlatev I, Barvik I, Geerts K, Leyssen P, Neyts J, Dutartre H, Canard B, Vasseur JJ, Morvan F, Alvarez K.

J Med Chem 53 6608-17 PMID:20799693
Generation of anti-DC-SIGN monoclonal antibodies capable of blocking HIV-1 gp120 binding and reactive on formalin-fixed tissue. (2010)

Canard B, Vachon H, Fontaine T, Pin JJ, Paul S, Genin C, Mueller CG.

Immunol Lett 5(1-2) 165-72 PMID:21078343
Structure and Functionality in Flavivirus Ns-Proteins: Perspectives for Drug Design. (2010)

Bollati M, Alvarez K, Assenberg R, Baronti C, Canard B, Cook S, Coutard B, Decroly E, De Lambellerie X, Gould EA, Grard G, Grimes JM, Hilgenfeld R, Jansson AM, Malet H, Mancini EJ, Mastrangelo E, Mattevi A, Milani M, Moureau G, Neyts J, Owens RJ, Ren J, Selisko B, Speroni S, Steuber H, Stuart DI, Unge T, Bolognesi M.

Antiviral Res 87(2) 125-48 PMID:19945487
The VIZIER Project: Overview; Expectations; and Achievements. (2010)

Coutard B, Canard B.

Antiviral Res 87(2) 85-94 PMID:20226212
High yield synthesis, purification and characterisation of the RNase L activators 5'-triphosphate 2'-5'-oligoadenylates. (2010)

Morin B, Rabah N, Boretto-Soler J, Tolou H, Alvarez K, Canard B.

Antiviral Res 87(3) 345-52 PMID:20547186
Preliminary insights into the non structural protein 3 macro domain of the Mayaro virus by powder diffraction (2010)

Papageorgiou N, Watier Y, Saunders L, Coutard B, Lantez V, Gould E.A, Fitch A.N, Wright J.P, Canard B, Margiolaki I.

Z. Kristallogr. 225 576-580 PMID:0
The 2C putative helicase of echovirus 30 adopts a hexameric ring-shaped structure (2010)

Papageorgiou N, Coutard B, Lantez V, Gautron E, Chauvet O, Baronti C, Norder H, de Lamballerie X, Heresanu N, Ferte N, Veesler S, Gorbalenya AE, Canard B.

Acta Crystallogr D Biol Crystallogr D66 1116-1120 PMID:20944244
The N-terminal domain of the arenavirus L protein is an RNA endonuclease essential in mRNA transcription. (2010)

Morin B, Coutard B, Lelke M, Ferron F, Kerber R, Jamal S, Frangeul A, Baronti C, Charrel R, de Lamballerie X, Vonrhein C, Lescar J, Bricogne G, Gunther S, Canard B.

PLoS Pathog 6 pii: e1001038 PMID:20862324
Genomics and structure/function studies of Rhabdoviridae proteins involved in replication and transcription. (2010)

Assenberg R, Delmas O, Morin B, Graham SC, De Lamballerie X, Laubert C, Coutard B, Grimes JM, Neyts J, Owens RJ, Brandt BW, Gorbalenya A, Tucker P, Stuart DI, Canard B, Bourhy H.

Antiviral Res 87 149-61 PMID:20188763
Practical application of bioinformatics by the multidisciplinary VIZIER consortium. (2010)

Gorbalenya AE, Lieutaud P, Harris M, Coutard B, Canard B, Kleywegt GK, Kravchenko AA, Samborskiy DV, Sidorov IA, Leontovich AM, Jones TA.

Antiviral Res 87(2): 95-110 PMID:20153379
Dengue virus replicons: production of an interserotypic chimera and cell lines from different species, and establishment of a cell-based fluorescent assay to screen inhibitors, validated by the evaluation of ribavirin's activity. (2010)

Masse N, Davidson A, Ferron F, Alvarez K, Jacobs M, Romette JL, Canard B, Guillemot JC.

Antiviral Res 86 296-305 PMID:20307577
Flexibility between the protease and helicase domains of the dengue virus NS3 protein conferred by the linker region and its functional implications. (2010)

Luo D, Wei N, Doan DN, Paradkar PN, Chong Y, Davidson AD, Kotaka M, Lescar J, Vasudevan SG.

J Biol Chem 285 18817-27 PMID:20375022
In vitro reconstitution of SARS-coronavirus mRNA cap methylation. (2010)

Bouvet M, Debarnot C, Imbert I, Selisko B, Snijder EJ, Canard B, Decroly E.

PLoS Pathog 6 e1000863 PMID:20421945
Conformational disorder. (2010)

Longhi S, Lieutaud P, Canard B.

Methods Mol Biol 609 307-25 PMID:20221927
Synthesis and antiviral activity of boranophosphonate isosteres of AZT and d4T monophosphates. (2010)

Barral K, Priet S, De Michelis C, Sire J, Neyts J, Balzarini J, Canard B, Alvarez K.

Eur J Med Chem 45 849-56 PMID:19969396
Biochemical characterization of the (nucleoside-2\'O)-methyltransferase activity of dengue virus protein NS5 using purified capped RNA oligonucleotides 7MeGpppACn and GpppACn. (2010)

Selisko B, Peyrane FF, Canard B, Alvarez K, Decroly E.

J Gen Virol 91 112-21 PMID:19776234

2009

Structure of the C-terminal domain of nsp4 from feline coronavirus. (2009)

Manolaridis I, Wojdyla JA, Panjikar S, Snijder EJ, Gorbalenya AE, Berglind H, Nordlund P, Coutard B, Tucker PA.

Acta Crystallogr D Biol Crystallogr 65 839-46 PMID:19622868
Understanding the alphaviruses: Recent research on important emerging pathogens and progress towards their control. (2009)

Gould EA, Coutard B, Malet H, Morin B, Jamal S, Weaver S, Gorbalenya A, Moureau G, Baronti C, Delogu I, Forrester N, Khasnatinov M, Gritsun T, de Lamballerie X, Canard B.

Antiviral Res 87(2) 111-24 PMID:19616028
Crystallographic structure of ubiquitin in complex with cadmium ions. (2009)

Qureshi IA, Ferron F, Seh CC, Cheung P, Lescar J.

BMC Res Notes 2 251 PMID:20003470
Overlapping genes produce proteins with unusual sequence properties and offer insight into de novo protein creation. (2009)

Rancurel C, Khosravi M, Dunker KA, Romero PR, Karlin D.

J Virol 83(20) 10719-36 PMID:19640978
Structure of the methyltransferase domain from the Modoc virus, a flavivirus with no known vector. (2009)

Jansson AM, Jakobsson E, Johansson P, Lantez V, Coutard B, de Lamballerie X, Unge T, Jones TA.

Acta Crystallogr D Biol Crystallogr 65 796-803 PMID:19622863
Structure of the X (ADRP) domain of nsp3 from feline coronavirus. (2009)

Wojdyla JA, Manolaridis I, Snijder EJ, Gorbalenya AE, Coutard B, Piotrowski Y, Hilgenfeld R, Tucker PA.

Acta Crystallogr D Biol Crystallogr 65 1292-300 PMID:19966415
Recognition of RNA Cap in the Wesselsbron Virus NS5 Methyltransferase Domain: Implications for RNA-Capping Mechanisms in Flavivirus. (2009)

Bollati M, Milani M, Mastrangelo E, Ricagno S, Tedeschi G, Nonnis S, Decroly E, Selisko B, de Lamballerie X, Coutard B, Canard B, Bolognesi M.

J Mol Biol 385 140-52 PMID:18976670
RNA-dependent RNA polymerases from flaviviruses and Picornaviridae. (2009)

Lescar J, Canard B.

Curr Opin Struct Biol 19 759-767 PMID:19910184
Monoclonal antibodies to the West Nile virus NS5 protein map to linear and conformational epitopes in the methyl transferase and RNA-dependant RNA polymerase domains. (2009)

Hall RA, Tan SE, Selisko B, Slade R, Hobson-Peters J, Canard B, Hughes M, Leung JY, Balmori Melian E, Hall-Mendelin S, Pham KB, Clark DC, Prow NA, Khromykh AA.

J Gen Virol 90 2912-2922 PMID:19710254
The Crystal Structures of Chikungunya and Venezuelan Equine Encephalitis Virus nsP3 Macro Domains Define a Conserved Adenosine Binding Pocket. (2009)

Malet H, Coutard B, Jamal S, Dutartre H, Papageorgiou N, Neuvonen M, Ahola T, Forrester N, Gould EA, Lafitte D, Ferron F, Lescar J, de Lamballerie X, Canard B.

J Virol 83(13) 6534-45 PMID:19386706
Identification of allosteric inhibitors blocking the Hepatitis C Virus polymerase NS5B in the RNA synthesis initiation step. (2009)

Betzi S, Eydoux C, Bussetta C, Blemont M, Leyssen P, Debarnot C, Ben-Rahou M, Haiech J, Hibert M, Gueritte F, Grierson DS, Romette JL, Guillemot JC, Neyts J, Alvarez K, Morelli X, Dutartre H, Canard B.

Antiviral Res 84 48-59 PMID:19589358
Flaviviral methyltransferase/RNA interaction: structural basis for enzyme inhibition. (2009)

Milani M, Mastrangelo E, Bollati M, Selisko B, Decroly E, Bouvet M, Canard B, Bolognesi M.

Antiviral Res 83 28-34 PMID:19501254
A pyrazolotriazolopyrimidinamine inhibitor of bovine viral diarrhea virus replication that targets the viral RNA-dependent RNA polymerase. (2009)

Paeshuyse J, Letellier C, Froeyen M, Dutartre H, Vrancken R, Canard B, De Clercq E, Gueiffier A, Teulade JC, Herdewijn P, Puerstinger G, Koenen F, Kerkhofs P, Baraldi PG, Neyts J.

Antiviral Res 82 141-7 PMID:19428605
Structural and Biochemical Analysis of Human Pathogenic Astrovirus Serine Protease at 2.0 A Resolution. (2009)

Speroni S, Rohayem J, Nenci S, Bonivento D, Robel I, Barthel J, Luzhkov VB, Coutard B, Canard B, Mattevi A.

J Mol Biol 387 1137-1152 PMID:19249313
Crystal structure of a Methyltransferase from a No-Known-Vector Flavivirus. (2009)

Bollati M, Milani M, Mastrangelo E, de Lambellarie X, Canard B, Bolognesi M.

Biochem Biophys Res Commun 382 200-204 PMID:19275894

2008

The flavivirus polymerase as a target for drug discovery. (2008)

Malet H, Masse N, Selisko B, Romette JL, Alvarez K, Guillemot JC, Tolou H, Yap TL, Vasudevan S, Lescar J, Canard B.

Antiviral Res 80 23-35 PMID:18611413
The crystal structure of coxsackievirus B3 RNA-dependent RNA polymerase in complex with its protein primer VPg confirms the existence of a second VPg binding site on Picornaviridae polymerases. (2008)

Gruez A, Selisko B, Roberts M, Bricogne G, Bussetta C, Jabafi I, Coutard B, De Palma AM, Neyts J, Canard B.

J Virol 82 9577-90 PMID:18632861
Towards the design of antiviral inhibitors against flaviviruses: The case for the multifunctional NS3 protein from Dengue virus as a target. (2008)

Lescar J, Luo D, Xu T, Sampath A, Lim SP, Canard B, Vasudevan SG.

Antiviral Res 80 94-101 PMID:18674567
MeDor: a metaserver for predicting protein disorder. (2008)

Lieutaud P, Canard B, Longhi S.

BMC Genomics 9 Suppl 2 S25 PMID:18831791
Acyclic phosphonates : molecular aspects of active site constraints within HIV-1 RT (2008)

Frangeul A, Busseta C, Deval J, Barral K,Selmi B, Boretto J, Alvarez K, Canard B.

Antivir Ther 13 (1) 115-24 PMID:18389906
Phosphoramidate Dinucleosides as Hepatitis C Virus Polymerase Inhibitors. (2008)

Zlatev I, Dutartre H, Barvik I, Neyts J, Canard B, Vasseur JJ, Alvarez K, Morvan F.

J Med Chem 51 5745-5757 PMID:18800766
The thiazolobenzimidazole TBZE-029 inhibits enteroviral replication by targeting a short region immediately downstream motif C in the non-structural protein 2C. (2008)

De Palma AM, Heggermont W, Lanke K, Coutard B, Bergmann M, Monforte AM, Canard B, De Clercq E, Chimirri A, Purstinger G, Rohayem J, van Kuppeveld F, Neyts J.

J Virol 82 4720-4730 PMID:18337578
Coronavirus nonstructural protein 16 is a cap-0 binding enzyme possessing (nucleoside-2'O)-methyltransferase activity. (2008)

Decroly E, Imbert I, Coutard B, Bouvet M, Selisko B, Alvarez K, Gorbalenya AE, Snijder EJ, Canard B.

J Virol 82 8071-8084 PMID:18417574
FUNCTIONAL CHARACTERIZATION OF THE CLEAVAGE SPECIFICITY OF THE SAPOVIRUS CHYMOTRYPSIN-LIKE PROTEASE. (2008)

Robel I, Gebhardt J, Mesters JR, Gorbalenya A, Coutard B, Canard B, Hilgenfeld R, Rohayem J.

J Virol 82 8085-8093 PMID:18550673
International research networks in viral structural proteomics: Again, lessons from SARS. (2008)

Canard B, Joseph JS, Kuhn P.

Antiviral Res 78 47-50 PMID:18054092
The VIZIER project: Preparedness against pathogenic RNA viruses. (2008)

Coutard B, Gorbalenya AE, Snijder EJ, Leontovich AM, Poupon A, De Lamballerie X, Charrel R, Gould EA, Gunther S, Norder H, Klempa B, Bourhy H, Rohayem J, L'hermite E, Nordlund P, Stuart DI, Owens RJ, Grimes JM, Tucker PA, Bolognesi M, Mattevi A, Coll M, Jones TA, Aqvist J, Unge T, Hilgenfeld R, Bricogne G, Neyts J, La Colla P, Puerstinger G, Gonzalez JP, Leroy E, Cambillau C, Romette JL, Canard B.

Antiviral Res 78 37-46 PMID:18083241
The SARS-Coronavirus PLnc domain of nsp3 as a replication/transcription scaffolding protein. (2008)

Imbert I, Snijder EJ, Dimitrova M, Guillemot JC, Lecine P, Canard B.

Virus Res 133 136-48 PMID:18255185
Structure and biochemical analysis of Kokobera virus helicase. (2008)

Speroni S, De Colibus L, Mastrangelo E, Gould E, Coutard B, Forrester NL, Blanc S, Canard B, Mattevi A.

Proteins 70 1120-3 PMID:18004778
The translation of recombinant proteins in E. coli can be improved by in silico generating and screening random libraries of a 70/+96 mRNA region with respect to the translation initiation codon. (2008)

Care S, Bignon C, Pelissier MC, Blanc E, Canard B, Coutard B.

Nucleic Acids Res 36 e6 PMID:18084029

2007

Predicting Protein Disorder and Induced Folding: From Theoretical Principles to Practical Applications. (2007)

Bourhis JM, Canard B, Longhi S.

Curr Protein Pept Sci 8 135-149 PMID:17430195
Le virus de la dengue : cibles virales et antiviraux. (2007)

Masse N, Selisko B, Malet H, Peyranne F, Debarnot C, Decroly E, Benarroch D, Egloff MP, Guillemot JC, Alvarez K, Canard B.

Virologie 11 121-130 PMID:0
Acyclic Phosphonate Nucleotides and Human Adenylate Kinases: Impact of a Borano Group on the Alpha-P Position (2007)

Topalis D, Alvarez K, Barral, Munier-Lehmann H, Schneider B, V�ron M, Guerreiro C, Mulard L, El-Amri C, Canard BDeville-Bonne D.

Nucleosides Nucleotides Nucleic Acids 27 319-31 PMID:18404568
Virtual screening and bioassay study of novel inhibitors for dengue virus mRNA cap (nucleoside-2'O)-methyltransferase. (2007)

Luzhkov VB, Selisko B, Nordqvist A, Peyrane F, Decroly E, Alvarez K, Karlen A, Canard B, Qvist J.

Bioorgan Med Chem 15 7795-7802 PMID:17888664
De novo initiation of RNA synthesis by the arterivirus RNA-dependent RNA polymerase. (2007)

Beerens N, Selisko B, Ricagno S, Imbert I, van der Zanden L, Snijder EJ, Canard B.

J Virol 81 8384-95 PMID:17537850
The imidazopyrrolopyridine analogue AG110 is a novel, highly selective inhibitor of pestiviruses that targets the viral RNA-dependent RNA polymerase at a hot spot for inhibition of viral replication. (2007)

Paeshuyse J, Chezal JM, Froeyen M, Leyssen P, Dutartre H, Vrancken R, Canard B, Letellier C, Li T, Mittendorfer H, Koenen F, Kerkhofs P, De Clercq E, Herdewijn P, Puerstinger G, Gueiffier A, Chavignon O, Teulade JC, Neyts J.

J Virol 81 11046-53 PMID:17686854
In vitro Suppression of K65R Reverse Transcriptase-mediated Tenofovir- and Adefovir-5' Diphosphate Resistance Conferred by the Borano-phosphonate Derivatives. (2007)

Frangeul A, Barral K, Alvarez K, Canard B.

Antimicrob Agents Chemother 51 3162-7 PMID:17620380
Crystal Structure and Activity of Kunjin Virus NS3 Helicase; Protease and Helicase Domain Assembly in the Full Length NS3 Protein. (2007)

Mastrangelo E, Milani M, Bollati M, Selisko B, Peyrane F, Pandini V, Sorrentino G, Canard B, Konarev PV, Svergun DI, de Lamballerie X, Coutard B, Khromykh AA, Bolognesi M.

J Mol Biol 372 444-55 PMID:17658551
Structural and Functional Analysis of Methylation and 5'-RNA Sequence Requirements of Short Capped RNAs by the Methyltransferase Domain of Dengue Virus NS5. (2007)

Egloff MP, Decroly E, Malet H, Selisko B, Benarroch D, Ferron F, Canard B.

J Mol Biol 372 723-36 PMID:17686489
High-yield production of short GpppA- and 7MeGpppA-capped RNAs and HPLC-monitoring of methyltransfer reactions at the guanine-N7 and adenosine-2primeO positions. (2007)

Peyrane F, Selisko B, Decroly E, Vasseur JJ, Benarroch D, Canard B, Alvarez K.

Nucleic Acids Res 35 e26 PMID:17259217
Improved crystallization of the coxsackievirus B3 RNA-dependent RNA polymerase. (2007)

Jabafi I, Selisko B, Coutard B, De Palma AM, Neyts J, Egloff MP, Grisel S, Dalle K, Campanacci V, Spinelli S, Cambillau C, Canard B, Gruez A.

Acta Crystallogr Sect F Struct Biol Cryst Commun 63 495-8 PMID:17554171
The crystal Structure of the Dengue virus RNA-dependent RNA polymerase catalytic domain at 1.85 A resolution. (2007)

Yap TL, Xu T, Chen YL, Malet H, Egloff MP, Canard B, Vasudevan SG, Lescar J.

J Virol 81 4753-4765 PMID:17301146
Structural bases for substrate recognition and activity in Meaban virus nucleoside-2'-O-methyltransferase. (2007)

Mastrangelo E, Bollati M, Milani M, Selisko B, Peyrane F, Canard B, Grard G, De Lamballerie X, Bolognesi M.

Protein Sci 16 1133-1145 PMID:17473012
Structural and functional characterization of Sapovirus RNA-dependent RNA polymerase (2007)

Fullerton SW, Blaschke M, Coutard B, Gebhardt J, Gorbalenya A, Canard B, Tucker PA, Rohayem J.

J Virol 81 1858-1871 PMID:17121797
Crystal structure of the RNA polymerase domain of the West Nile virus non-structural protein 5. (2007)

Malet H, Egloff MP, Selisko B, Butcher RE, Wright PJ, Roberts M, Gruez A, Sulzenbacher G, Vonrhein C, Bricogne G, Mackenzie JM, Khromykh AA, Davidson AD, Canard B.

J Biol Chem 282 10678-10689 PMID:17287213
Purification and crystallization of Kokobera virus helicase. (2007)

De Colibus L, Speroni S, Coutard B, Forrester NL, Gould E, Canard B, Mattevi A.

Acta Crystallogr Sect F Struct Biol Cryst Commun 63 193-5 PMID:17329812

2006

The Impact of Protein Characterisation in Structural Proteomics. (2006)

Geerlof A, Brown J, Coutard B, Egloff MP, Enguita FJ, Fogg MJ, Gilbert RJ, Groves MR, Haouz A, Nettleship JE, Nordlund P, Owens RJ, Ruff M, Sainsbury S, Svergun DI, Wilmanns M..

Acta Crystallogr D Biol Crystallogr 62 1125-36 PMID:17001090
Assessing protein disorder and induced folding. (2006)

Receveur-Brechot V, Bourhis JM, Uversky VN, Canard B, Longhi S.

Proteins 62 24-45 PMID:16287116
Structural disorder within the replicative complex of measles virus: Functional implications. (2006)

Bourhis JM, Canard B, Longhi S.

Virology 344 94-110 PMID:16364741
A practical overview of protein disorder prediction methods. (2006)

Ferron F, Longhi S, Canard B, Karlin D.

Proteins 65 1-14 PMID:16856179
Discovery of an RNA virus 3' > 5' exoribonuclease that is critically involved in coronavirus RNA synthesis. (2006)

Minskaia E, Hertzig T, Gorbalenya AE, Campanacci V, Cambillau C, Canard B, Ziebuhr J.

Proc Natl Acad Sci U S A 103 5108-13 PMID:16549795
Crystal Structure and Mechanistic Determinants of SARS Coronavirus Nonstructural Protein 15 Define a Novel Endoribonuclease Family (2006)

Ricagno S, Egloff MP, Ulferts R, Coutard B, Nurizzo D, Campanacci V, Cambillau C, Ziebuhr J, Canard B.

Proc Natl Acad Sci U S A 103 11892-7 PMID:16882730
Preliminary characterization of (nucleoside-2'-O-)-methyltransferase crystals from Meaban and Yokose flaviviruses. (2006)

Mastrangelo E, Bollati M, Milani M, de Lamballerie X, Brisbarre N, Dalle K, Lantez V, Egloff MP, Coutard B, Canard B, Gould E, Forrester N, Bolognesi M.

Acta Crystallogr Sect F Struct Biol Cryst Commun 62 768-70 PMID:16880552
Analogues de nucléotides dérivés du H-phosphinate du PMEA et PMPA comme molécules précurseurs d'antiviraux. Exemple : Découverte de thiophosphonates actifs contre le VIH et le VHB (2006)

Alvarez K, Barral K, Balzarini J, Canard B, Neyts J, Romette JL .

Brevet déposé en octobre 2006. N DV : DI 00941-01. Dépôt de brevet français au nom du CNRS à titre conservatoire. N 06/09749. PMID:0
Structural and Functional Basis for ADP-ribose and Poly(ADP-Ribose) binding by Viral Macro Domains (2006)

Egloff MP, Malet H, Putics A, Heinonen M, Dutartre H, Frangeul A, Gruez A, Campanacci V, Cambillau C, Zieebuhr J, Ahola T, Canard B.

J Virol 80 8493-502 PMID:16912299
General catalytic deficiency of hepatitis C virus RNA polymerase with an S282T mutation and mutually exclusive resistance towards 2'-modified nucleotide analogues. (2006)

Dutartre H, Bussetta C, Boretto J, Canard B.

Antimicrob Agents Chemother 50 4161-9 PMID:17000745
Synthesis, in vitro antiviral evaluation and stability studies of novel a-borano-nucleotide analogues of 9-[2-(Phosphonomethoxy)ethyl]adenine (PMEA) and (R)-9-[2-(Phosphonomethoxy)propyl]adenine (PMPA) (2006)

Barral K, Priet S, Sire J, Neyts J, Balzarini J, Canard B, Alvarez K.

J Med Chem 49 7799-7806 PMID:17181162
A second, non-canonical RNA-dependent RNA polymerase in SARS Coronavirus (2006)

Imbert I, Guillemot JC, Bourhis JM, Bussetta C, Coutard B, Egloff MP, Ferron F, Gorbalenya AE, Canard B.

EMBO J 25 4933-4942 PMID:17024178
Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens. (2006)

Fogg MJ, Alzari P, Bahar M, Bertini I, Betton JM, Burmeister WP, Cambillau C, Canard B, Carrondo M, Coll M, Daenke S, Dym O, Egloff MP, Enguita FJ, Geerlof A, Haouz A, Jones TA, Ma Q, Manicka SN, Migliardi M, Nordlund P, Owens RJ, Peleg Y, Schneider G, Schnell R, Stuart DI, Tarbouriech N, Unge T, Wilkinson AJ, Wilmanns M, Wilson KS, Zimhony O, Grimes JM.

Acta Crystallogr D Biol Crystallogr 62 1196-207 PMID:17001096
SPINE bioinformatics and data-management aspects of high-throughput structural biology. (2006)

Albeck S, Alzari P, Andreini C, Banci L, Berry IM, Bertini I, Cambillau C, Canard B, Carter L, Cohen SX, Diprose JM, Dym O, Esnouf RM, Felder C, Ferron F, Guillemot F, Hamer R, Ben Jelloul M, Laskowski RA, Laurent T, Longhi S, Lopez R, Luchinat C, Malet H, Mochel T, Morris RJ, Moulinier L, Oinn T, Pajon A, Peleg Y, Perrakis A, Poch O, Prilusky J, Rachedi A, Ripp R, Rosato A, Silman I, Stuart DI, Sussman JL, Thierry JC, Thompson JD, Thornton JM, Unger T, Vaughan B, Vranken W, Watson JD, Whamond G, Henrick K.

Acta Crystallogr D Biol Crystallogr 62 1184-95 PMID:17001095
Preliminary crystallographic characterization of an RNA helicase from Kunjin virus. (2006)

Mastrangelo E, Bollati M, Milani M, Brisbarre N, de Lamballerie X, Coutard B, Canard B, Khromykh A, Bolognesi M.

Acta Crystallogr Sect F Struct Biol Cryst Commun 62 876-9 PMID:16946468
Comparative mechanistic studies of de novo RNA synthesis by flavivirus RNA-dependent RNA polymerases. (2006)

Selisko B, Dutartre H, Guillemot JC, Debarnot C, Benarroch D, Khromykh A, Despres P, Egloff MP, Canard B.

Virology 351 145-58 PMID:16631221
The non-structural protein Nsp10 of mouse hepatitis virus binds zinc ions and nucleic acids. (2006)

Matthes N, Mesters JR, Coutard B, Canard B, Snijder EJ, Moll R, Hilgenfeld R.

FEBS Lett 580 4143-9 PMID:16828088
Synthesis and anti-BVDV activity of acridones as new potential antiviral agents. (2006)

Tabarrini O, Manfroni G, Fravolini A, Cecchetti V, Sabatini S, De Clercq E, Rozenski J, Canard B, Dutartre H, Paeshuyse J, Neyts J.

J Med Chem 49 2621-7 PMID:16610805
Crystallization and preliminary X-ray diffraction analysis of Nsp15 from SARS coronavirus. (2006)

Ricagno S, Coutard B, Grisel S, Bremond N, Dalle K, Tocque F, Campanacci V, Lichiere J, Lantez V, Debarnot C, Cambillau C, Canard B, Egloff MP.

Acta Crystallogr Sect F Struct Biol Cryst Commun 62 409-411 PMID:16582498
Expression, purification and crystallization of the SARS-CoV macro domain. (2006)

Malet H, Dalle K, Bremond N, Tocque F, Blangy S, Campanacci V, Coutard B, Grisel S, Lichiere J, Lantez V, Cambillau C, Canard B, Egloff MP.

Acta Crystallogr Sect F Struct Biol Cryst Commun 62 405-8 PMID:16582497
Virologic and enzymatic studies revealing the mechanism of K65R- and Q151m-associated HIV-1 drug resistance towards emtricitabine and lamivudine. (2006)

Feng JY, Myrick FT, Margot NA, Mulamba GB, Rimsky L, Borroto-Esoda K, Selmi B, Canard B.

Nucleosides Nucleotides Nucleic Acids 25 89-107 PMID:16440988
A Novel, Highly Selective Inhibitor of Pestivirus Replication That Targets the Viral RNA-Dependent RNA Polymerase. (2006)

Paeshuyse J, Leyssen P, Mabery E, Boddeker N, Vrancken R, Froeyen M, Ansari IH, Dutartre H, Rozenski J, Gil LH, Letellier C, Lanford R, Canard B, Koenen F, Kerkhofs P, Donis RO, Herdewijn P, Watson J, De Clercq E, Puerstinger G, Neyts J.

J Virol 80 149-60 PMID:16352539

2005

Borano-nucleotides: new analogues to circumvent HIV-1 RT-mediated nucleoside drug-resistance. (2005)

Alvarez K, Deval J, Selmi B, Barral K, Boretto J, Guerreiro C, Mulard L, Sarfati R, Canard B.

Nucleosides Nucleotides Nucleic Acids 24 419-21 PMID:16247962
The modeled structure of the RNA dependent RNA polymerase of GBV-C Virus suggests a role for motif E in Flaviviridae RNA polymerases. (2005)

Ferron F, Bussetta C, Dutartre H, Canard B.

BMC Bioinformatics 6 255 PMID:16225688
The intrinsically disordered C-terminal domain of the measles virus nucleoprotein interacts with the C-terminal domain of the phosphoprotein via two distinct sites and remains predominantly unfolded. (2005)

Bourhis JM, Receveur-Brechot V, Oglesbee M, Zhang X, Buccellato M, Darbon H, Canard B, Finet S, Longhi S.

Protein Sci 14 1975-92 PMID:16046624
Essential amino acids of the hantaan virus N protein in its interaction with RNA. (2005)

Severson W, Xu X, Kuhn M, Senutovitch N, Thokala M, Ferron F, Longhi S, Canard B, Jonsson CB.

J Virol 79 10032-9 PMID:16014963
Effects of HIV Q151M-associated multi-drug resistance mutations on the activities of (-)-beta-d-1',3'-dioxolan guanine. (2005)

Feng JY, Myrick F, Selmi B, Deval J, Canard B, Borroto-Esoda K.

Antiviral Res 66 153-8 PMID:15885814
Suppression of multidrug-resistant HIV-1 reverse transcriptase primer unblocking activity by alpha-phosphate-modified thymidine analogues. (2005)

Matamoros T, Deval J, Guerreiro C, Mulard L, Canard B, Menendez-Arias L.

J Mol Biol 349 451-63 PMID:15878178
Hsp72 recognizes a P binding motif in the measles virus N protein C-terminus. (2005)

Zhang X, Bourhis JM, Longhi S, Carsillo T, Buccellato M, Morin B, Canard B, Oglesbee M.

Virology 337 162-74 PMID:15914229
Measles virus nucleoprotein induces cell-proliferation arrest and apoptosis through NTAIL-NR and NCORE-FcgammaRIIB1 interactions, respectively. (2005)

Laine D, Bourhis JM, Longhi S, Flacher M, Cassard L, Canard B, Sautes-Fridman C, Rabourdin-Combe C, Valentin H.

J Gen Virol 86 1771-84 PMID:15914856
VaZyMolO: a tool to define and classify modularity in viral proteins. (2005)

Ferron F, Rancurel C, Longhi S, Cambillau C, Henrissat B, Canard B.

J Gen Virol 86 743-749 PMID:15722535
A relaxed discrimination of 2'-O-methyl GTP relative to GTP between de novo and elongative RNA synthesis by the hepatitis C RNA-dependent RNA polymerase NS5B (2005)

Dutartre H, Boretto J, Gullemot JC, Canard B.

J Biol Chem 280 6359-68 PMID:15561709
HIV-1-Associated Uracil DNA Glycosylase Activity Controls dUTP Misincorporation in Viral DNA and Is Essential to the HIV-1 Life Cycle. (2005)

Priet S, Gros N, Navarro JM, Boretto J, Canard B, Querat G, Sire J.

Mol Cell 17 479-90 PMID:15721252
Mechanistic insights into the suppression of drug-resistance by human immunodeficiency virus type-1 reverse transcriptase using alpha-boranophosphate nucleoside analogues. (2005)

Deval J, Alvarez K, Selmi B, Bermond M, Boretto J, Guerreiro C, Mulard L, Canard B.

J Biol Chem 280 3838-46 PMID:15550379

2004

The C-terminal domain of measles virus nucleoprotein belongs to the class of intrinsically disordered proteins that fold upon binding to their physiological partner. (2004)

Bourhis JM, Johansson K, Receveur-Brechot V, Oldfield CJ, Dunker KA, Canard B, Longhi S.

Virus Res 99 157-167 PMID:14749181
The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world (2004)

Egloff MP, Ferron F, Campanacci V, Longhi S, Rancurel C, Dutartre H,Snijder EJ, Gorbalenya AE, Cambillau C, Canard B.

Proc Natl Acad Sci U S A 101 3792-2796 PMID:15007178
The RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activities of Dengue virus protein NS3 are Mg(2+)-dependent and require a functional Walker B motif in the helicase catalytic core. (2004)

Benarroch D, Selisko B, Locatelli GA, Maga G, Romette JL, Canard B.

Virology 328 208-18 PMID:15464841
A loss of viral replicative capacity correlates with altered DNA polymerization kinetics by the human immunodeficiency virus reverse transcriptase bearing the K65R and L74V dideoxynucleoside resistance substitutions (2004)

Deval J, Navarro JM, Selmi B, Courcambeck J, Boretto J, Halfon P, Garrido-Urbani S, Sire J, Canard B.

J Biol Chem 279 25489-25496 PMID:15044478
A structural basis for the inhibition of the NS5 dengue virus mRNA 2'-O-methyltransferase domain by Ribavirin 5'-triphosphate. (2004)

Benarroch D, Egloff MP, Mulard L, Guerreiro C, Romette JL, Canard B.

J Biol Chem 279 35638-35643 PMID:15152003
Structural Determinants and Molecular Mechanisms for the Resistance of HIV-1 RT to Nucleoside Analogues. (2004)

Deval J, Courcambeck J, Selmi B, Boretto J, Canard B.

Curr Drug Metab 5 305-16 PMID:15320702
Mechanistic basis for reduced viral and enzymatic fitness of HIV-1 reverse transcriptase containing both K65R and M184V mutations. (2004)

Deval J, White KL, Miller MD, Parkin NT, Courcambeck J, Halphon P, Selmi B, Boretto J, Canard B.

J Biol Chem 279 509-516 PMID:14551187

2003

Structural disorder and modular organization in Paramyxovirinae N and P. (2003)

Karlin D, Ferron F, Canard B, Longhi S.

J Gen Virol 84 3239-52 PMID:14645906
Crystal structure of the measles virus phosphoprotein domain responsible for the induced folding of the C-terminal domain of the nucleoprotein. (2003)

Johansson K, Bourhis JM, Campanacci V, Cambillau C, Canard B, Longhi S.

J Biol Chem 278 44567-73 PMID:12944395
Structural genomics of the SARS coronavirus: cloning, expression, crystallization and preliminary crystallographic study of the Nsp9 protein. (2003)

Campanacci V, Egloff MP, Longhi S, Ferron F, Rancurel C, Salomoni A, Durousseau C, Tocque F, Bremond N, Dobbe JC, Snijder EJ, Canard B, Cambillau C.

Acta Crystallogr D Biol Crystallogr 59 1628-31 PMID:12925794
Structural Analysis of the Activation of Ribavirin Analogs by NDP Kinase: Comparison with Other Ribavirin Targets. (2003)

Gallois-Montbrun S, Chen Y, Dutartre H, Sophys M, Morera S, Guerreiro C, Schneider B, Mulard L, Janin J, Veron M, Deville-Bonne D, Canard B.

Mol Pharmacol 63 538-546 PMID:12606760
The Y181C substitution in AZT-resistant human immunodeficiency virus type 1 reverse transcriptase suppresses the ATP-mediated repair of the AZTMP-terminated primer. (2003)

Selmi B, Deval J, Alvarez K, Boretto J, Sarfati S, Guerreiro C, Canard B.

J Biol Chem 278 40464-40472 PMID:12902345
Measles Virus (MV) Nucleoprotein Binds to a Novel Cell Surface Receptor Distinct from FcgammaRII via Its C-Terminal Domain: Role in MV-Induced Immunosuppression. (2003)

Laine D, Trescol-Biemont MC, Longhi S, Libeau G, Marie JC, Vidalain PO, Azocar O, Diallo A, Canard B, Rabourdin-Combe C, Valentin H.

J Virol 77 11332-11346 PMID:14557619
Nucleotide analogue binding, catalysis and primer unblocking in the mechanisms of HIV-1 reverse transcriptase-mediated resistance to nucleoside analogues. (2003)

Selmi B, Deval J, Boretto J, Canard B.

Antivir Ther 8 143-54 PMID:12741627
The C-terminal domain of the measles virus nucleoprotein is intrinsically disordered and folds upon binding to the C-terminal moiety of the phosphoprotein. (2003)

Longhi S, Receveur-Brechot V, Karlin D, Johansson K, Darbon H, Bhella D, Yeo R, Finet S, Canard B.

J Biol Chem 278 18638-48 PMID:12621042

2002

An RNA cap (nucleoside-2'-O-)-methyltransferase in the flavivirus RNA polymerase NS5: crystal structure and functional characterization. (2002)

Egloff MP, Benarroch D, Selisko B, Romette JL, Canard B..

EMBO J 21 2757-2768 PMID:12032088
The molecular mechanism of multidrug resistance by the Q151M human immunodeficiency virus reverse transcriptase, and its suppression using a-boranophosphate nucleotide analogues. (2002)

Deval J, Selmi B, Boretto J, Egloff MP, Guerreiro C, Sarfati SR, Canard B.

J Biol Chem 277 42097-42104 PMID:12194983
Substitution of two residues in the measles virus nucleoprotein results in an impaired self-association. (2002)

Karlin D, Longhi S, Canard B.

Virology 302 420-432 PMID:12441086
The N-terminal domain of the phosphoprotein of Morbilliviruses belongs to the natively unfolded class of proteins. (2002)

Karlin D, Longhi S, Receveur V, Canard B.

Virology 296 251-262 PMID:12069524
Viral RNA-polymerases - a predicted 2'-O-ribose methyltransferase domain shared by all Mononegavirales (2002)

Ferron F, Longhi S, Henrissat B, Canard B.

Trends Biochem Sci 27 222-224 PMID:12076527

2001

The Valine-to-Threonine 75 Substitution in Human Immunodeficiency Virus Type 1 Reverse Transcriptase and its Relation with Stavudine Resistance (2001)

Selmi B, Boretto J, Navarro JM, Sire J, Longhi S, Guerreiro C,Mulard L,Sarfati S,Canard B.

J Biol Chem 276 13965-13974 PMID:11134027
An integrated system to study multiply-substituted HIV-1 reverse transcriptase (2001)

Boretto J, Longhi S, Navarro JM, Selmi B, Sire J and Canard B.

Anal Biochem 292 139-147 PMID:11319828
Human monocytes possess a serine protease activity capable of degrading hiv-1 reverse transcriptase in vitro. (2001)

Chateau MT, Robert-Hebmann V, Devaux C, Lazaro JB, Canard B, Coux O.

Biochem Biophys Res Commun 285 863-872 PMID:11467830
Activation of anti-reverse transcriptase nucleotide analogs by nucleoside diphosphate kinase: improvement by alpha-boranophosphate substitution. (2001)

Schneider B, Meyer P, Sarfati S, Mulard L, Guerreiro C, Boretto J, Janin J, Veron M, Deville-Bonne D, Canard B..

Nucleosides Nucleotides Nucleic Acids 20 297-306 PMID:11563041
La mesure de l'inhibition des transcriptases inverses du VIH-1 résistantes aux drogues nucléosidiques (2001)

Selmi B, Boretto J, Canard B.

Virologie 5 361-365 PMID:0
Mechanism-Based Suppression of Dideoxynucleotide Resistance by K65R Human Immunodeficiency Virus Reverse Transcriptase Using an a-Boranophosphate Nucleoside Analogue (2001)

Selmi B, Boretto J,Sarfati SR, Guerreiro C, Canard B.

J Biol Chem 276 48466-48472 PMID:11606579
Glutamic residue 438 within the protease-sensitive subdomain of HIV-1 reverse transcriptase is critical for heterodimer processing in viral particles. (2001)

Navarro JM, Damier L, Boretto J, Priet S, Canard B, Querat G, Sire J..

Virology 290 300-308 PMID:11883194

2000

Structural basis for activation of alpha-boranophosphate nucleotide analogues targeting drug-resistant reverse transcriptase. (2000)

Meyer P, Schneider B, Sarfati S, Deville-Bonne D, Guerreiro C, Boretto J, Janin J, Veron M, Canard B.

EMBO J 19 3520-3529 PMID:10899107
Phosphorylation of AZT-resistant human immunodeficiency virus type 1 reverse transcriptase by casein kinase II in vitro: effects on inhibitor sensitivity. (2000)

Lazaro JB, Boretto J, Selmi B, Capony JP, Canard B..

Biochem Biophys Res Commun 275 26-32 PMID:10944435

1999

The motif D loop of human immunodeficiency virus type I reverse transcriptase is critical for nucleoside 5' triphosphate selectivity. (1999)

Canard B, Chowdhury K, Sarfati R, Doublie S and Richardson CC.

J Biol Chem 274 35768-76 PMID:10585459
Mecanisme de transcription et replication des Paramyxoviridae (1999)

Longhi S, Canard B.

Virologie 3 227-240 PMID:0