Technology

This section's articles

• Surface Plasmon Resonance

  14 October 2011, by Maria MATE

  Biacore systems generate data on interaction between proteins and other molecules, such as other proteins, nucleic acids or small molecules. This technique exploits the phenomena of surface plasmon resonance (SPR). One of the molecules of the binding pair will be considered as the "ligand" and will be immobilised on the surface of the sensor chip. A solution with the other partner of the interaction (called the "analyte") will be injected over the immobilised ligand. Changes in the index (...)

  Read more of Surface Plasmon Resonance

• Isothermal Titration Calorimetry

  14 October 2011, by Maria MATE

  Introduction Isothermal Titration Calorimetry (ITC) is a powerful analytical tool that allows the measurement of binding affinities and of their related thermodynamic parameters. ITC directly measures the heat released or absorbed during molecular binding. Measurement of this heat enables direct accurate determination of a number of thermodynamic parameters in a single experiment: binding affinity (KD), stoichiometry (n), enthalpy (ΔH). From the former and the latter of these parameters we (...)

  Read more of Isothermal Titration Calorimetry

• Screening Platform Marseille-Luminy (PCML)

  1 December 2011, by Karine ALVAREZ, Karine BARRAL, Bruno CANARD, Cécilia EYDOUX, Jean-Claude GUILLEMOT, Barbara SELISKO, Laetitia STUHL-GOURMAND

  Introduction The screening platform Marseille-Luminy (PCML) is involved in antiviral research and belongs to the Antiviral Drug Design Platform (AD2P). A collaborative network is on track since 2005 between the screening platform Marseille-Luminy (PCML-JC Guillemot), the screening platform on viruses Marseille-Timone (PCVMT-G.Querat) and the Interactions, Dynamics and Drug Design platform (INT-3D-X. Morelli). (for further information, see : www. ad2p….) Next : Activities on PCML (...)

  Read more of Screening Platform Marseille-Luminy (PCML)

• Multi-Angle Light Scattering coupled to Size-Exclusion Chromatography (MALS/SEC)

  28 February 2012, by Stéphanie BLANGY

  The Wyatt technology Wyatt-HPLC system Size-exclusion chromatography with an on-line MiniDAWN TREOS triple-angle light-scattering detector coupled to a Dynapro quasi-elastic light-scattering instrument and an Optilab rEX differential refractometer. Examples of applications: Determination of the hydrodynamic radii (Rh), gyration radii (Rg), and the molecular size in solution of individual protein species Determination of the oligomerization states of proteins Study of glycosylation and (...)

  Read more of Multi-Angle Light Scattering coupled to Size-Exclusion Chromatography (MALS/SEC)

• Cloning, expression, purification of recombinant proteins

  11 December 2012, by Renaud VINCENTELLI

  Introduction The cloning, expression and protein purification facility of the AFMB (IBiSA) is opened to customers since 2003 and is linked to the protein characterization and crystallography facilities of the lab. References We are using our own high-throughput strategies and methods which were developped and validated for our structural genomics projects since 2001 (ASG, X-TB, SPINE, SPINE II, EMEP, MEPNET, VIZIER, VENOMICS (www.venomics.eu)...). We have the state-of-the-art equipment (...)

  Read more of Cloning, expression, purification of recombinant proteins