We focus mainly on positive strand RNA viruses. We use integrated multidisciplinary approaches in order to produce recombinant enzymes, characterize their enzymatic activities, define their biophysical properties and resolve their tridimensional structure. All these approaches allowed us to identify new targets for antiviral drugs design. The laboratory has chemical libraries and access to the screening platform "PCML" and "AD2P" to identify inhibitory molecules.
Some references
Single pH buffer refolding screen for protein from inclusion bodies. (2012)
Coutard B, Danchin EG, Oubelaid R, Canard B, Bignon C
Protein Expr Purif 82 : 352-359
Alkylated Flavanones from the Bark of Cryptocarya chartacea As Dengue Virus NS5 Polymerase Inhibitors. (2011)
Allard PM, Dau ET, Eydoux C, Guillemot JC, Dumontet V, Poullain C, Canard B, Gueritte F, Litaudon M
J Nat Prod 74 : 2446-53
Flavivirus NS3 and NS5 proteins interaction network: A high-throughput yeast two-hybrid screen. (2011)
Le Breton M, Meyniel-Schicklin L, Deloire A, Coutard B, Canard B, de Lamballerie X, Andre P, Rabourdin-Combe C, Lotteau V, Davoust N
BMC Microbiol 11 : 234
Practical application of bioinformatics by the multidisciplinary VIZIER consortium. (2010)
Gorbalenya AE, Lieutaud P, Harris M, Coutard B, Canard B, Kleywegt GK, Kravchenko AA, Samborskiy DV, Sidorov IA, Leontovich AM, Jones TA
Antiviral Res 87(2): : 95-110
MeDor: a metaserver for predicting protein disorder. (2008)
Lieutaud P, Canard B, Longhi S
BMC Genomics 9 Suppl 2 : S25
A practical overview of protein disorder prediction methods. (2006)
Ferron F, Longhi S, Canard B, Karlin D
Proteins 65 : 1-14
VaZyMolO: a tool to define and classify modularity in viral proteins. (2005)
Ferron F, Rancurel C, Longhi S, Cambillau C, Henrissat B, Canard B
J Gen Virol 86 : 743-749
