Latest Publications
- High resolution cryo-EM structure of the helical RNA-bound Hantaan virus nucleocapsid reveals its assembly mechanisms. (2019) Arragain B, Reguera J, Desfosses A, Gutsche I, Schoehn G, Malet H. Elife 8 PMID:30638449
- Discovery of novel carbohydrate-active enzymes through the rational exploration of the protein sequences space. (2019) Helbert W, Poulet L, Drouillard S, Mathieu S, Loiodice M, Couturier M, Lombard V, Terrapon N, Turchetto J, Vincentelli R, Henrissat B. Proc Natl Acad Sci U S A 116 6063-6068 PMID:30850540
- Rewiring of RSK-PDZ Interactome by Linear Motif Phosphorylation. (2019) Gogl G, Biri-Kovacs B, Durbesson F, Jane P, Nomine Y, Kostmann C, Bilics V, Simon M, Remenyi A, Vincentelli R, Trave G, Nyitray L. J Mol Biol in press PMID:30726710
- A gated relaxation oscillator mediated by FrzX controls morphogenetic movements in Myxococcus xanthus. (2018) Guzzo M, Murray SM, Martineau E, Lhospice S, Baronian G, My L, Zhang Y, Espinosa L, Vincentelli R, Bratton BP, Shaevitz JW, Molle V, Howard M, Mignot T,. Nat Microbiol 3 948-959 PMID:30013238
- Transcription and replication mechanisms of Bunyaviridae and Arenaviridae L proteins. (2017) Ferron F, Weber F, de la Torre JC, Reguera J. Virus Res 234 118-134 PMID:28137457
- Structural insights into reptarenavirus cap-snatching machinery. (2017) Rosenthal M, Gogrefe N, Vogel D, Reguera J, Rauschenberger B, Cusack S, Gunther S, Reindl S. PLoS Pathog 13 e1006400 PMID:28505175
Our team focuses on understanding viral infection mechanisms often ruled by the interplay of viral proteins, cellular proteins and nucleic acids assembled into multifunctional complexes. The aim is to understand the mechanisms by which the multiple enzymatic activities are coordinated between them and regulated to efficiently carry out infection. To reach our goals we use a broad range of biochemical and biophysical technics combined with X-ray crystallography and electron microscopy.
The mainstream of our research is the functional and structural characterization of the alphavirus replication complex. Alphavirus, like chikungunya virus (CHIK) or eastern equine encephalitis virus (EEEV), cause zoonotic infectious diseases with high impact in global human health. The viral genome replication and gene expression is carried out by the multifunctional viral replication complex. Interestingly this complex can adopt different functions depending on its state of maturation. Our goal is to structurally and functionally characterize the different functional states of the replication complex in order to propose an integrative mechanistic model accurately describing at the molecular level the viral infection cycle and its regulation. The results will provide invaluable insights and tools for the development of new strategies for the treatment of these concerning infectious diseases.
