Latest Publications
- Community-Wide Experimental Evaluation of the PROSS Stability-Design Method (2021) Peleg Y, Vincentelli R, Collins BM, Chen KE, Livingstone EK, Weeratunga S, Leneva N, Guo Q, Remans K, Perez K, Bjerga GEK, Larsen O, Vanek O, Skorepa O, Jacquemin S, Poterszman A, Kjaer S, Christodoulou E, Albeck S, Dym O, Ainbinder E, Unger T, Schuetz A, Matthes S, Bader M, de Marco A, Storici P, Semrau MS, Stolt-Bergner P, Aigner C, Suppmann S, Goldenzweig A, Fleishman SJ. J Mol Biol 433 166964 PMID:33781758
- Host PDZ-containing proteins targeted by SARS-Cov-2 (2021) Caillet-Saguy C, Durbesson F, Rezelj VV, Gogl G, Dinh Tran Q, Twizere JC, Vignuzzi M, Vincentelli R, Wolff N. FEBS J in press PMID:33864728
- Structural insights into the mechanisms of action of functionally distinct classes of Chikungunya virus nonstructural protein 1 inhibitors (2021) Kovacikova K, Gonzalez MG, Jones R, Reguera J, Gigante A, Perez-Perez MJ, Purstinger G, Moesslacher J, Langer T, Jeong LS, Delang L, Neyts J, Snijder EJ, van Westen GJP, van Hemert MJ. Antimicrob Agents Chemother 65 e0256620 PMID:33875421
- A High-Affinity Peptide Ligand Targeting Syntenin Inhibits Glioblastoma (2021) Haugaard-Kedstrom LM, Clemmensen LS, Sereikaite V, Jin Z, Fernandes EFA, Wind B, Abalde-Gil F, Daberger J, Vistrup-Parry M, Aguilar-Morante D, Leblanc R, Egea-Jimenez AL, Albrigtsen M, Jensen KE, Jensen TMT, Ivarsson Y, Vincentelli R, Hamerlik P, Andersen JH, Zimmermann P, Lee W, Stromgaard K. J Med Chem 64 1423-1434 PMID:33502198
- Capping pores of alphavirus nsP1 gate membranous viral replication factories (2021) Jones R, Bragagnolo G, Arranz R, Reguera J. Nature 589 615-619 PMID:33328629
- Interactomic affinity profiling by holdup assay: Acetylation and distal residues impact the PDZome-binding specificity of PTEN phosphatase (2020) Jane P, Gogl G, Kostmann C, Bich G, Girault V, Caillet-Saguy C, Eberling P, Vincentelli R, Wolff N, Trave G, Nomine Y. PLoS One 15 e0244613 PMID:33382810
Our team focuses on understanding viral infection mechanisms often ruled by the interplay of viral proteins, cellular proteins and nucleic acids assembled into multifunctional complexes. The aim is to understand the mechanisms by which the multiple enzymatic activities are coordinated between them and regulated to efficiently carry out infection. To reach our goals we use a broad range of biochemical and biophysical technics combined with X-ray crystallography and electron microscopy.
The mainstream of our research is the functional and structural characterization of the alphavirus replication complex. Alphavirus, like chikungunya virus (CHIK) or eastern equine encephalitis virus (EEEV), cause zoonotic infectious diseases with high impact in global human health. The viral genome replication and gene expression is carried out by the multifunctional viral replication complex. Interestingly this complex can adopt different functions depending on its state of maturation. Our goal is to structurally and functionally characterize the different functional states of the replication complex in order to propose an integrative mechanistic model accurately describing at the molecular level the viral infection cycle and its regulation. The results will provide invaluable insights and tools for the development of new strategies for the treatment of these concerning infectious diseases.
