Latest Publications
- Capping pores of alphavirus nsP1 gate membranous viral replication factories (2020) Jones R, Bragagnolo G, Arranz R, Reguera J. Nature In press PMID:33328629
- A Venomics Approach Coupled to High-Throughput Toxin Production Strategies Identifies the First Venom-Derived Melanocortin Receptor Agonists (2020) Reynaud S, Ciolek J, Degueldre M, Saez NJ, Sequeira AF, Duhoo Y, Bras JLA, Meudal H, Cabo Diez M, Fernandez Pedrosa V, Verdenaud M, Boeri J, Pereira Ramos O, Ducancel F, Vanden Driessche M, Fourmy R, Violette A, Upert G, Mourier G, Beck-Sickinger AG, Morl K, Landon C, Fontes CMGA, Minambres Herraiz R, Rodriguez de la Vega RC, Peigneur S, Tytgat J, Quinton L, De Pauw E, Vincentelli R, Servent D, Gilles N. J Med Chem 63 8250-8264 PMID:32602722
- Pre-initiation and elongation structures of full-length La Crosse virus polymerase reveal functionally important conformational changes (2020) Arragain B, Effantin G, Gerlach P, Reguera J, Schoehn G, Cusack S, Malet H. Nat Commun 11 3590 PMID:32681014
- Structure and function of the Toscana virus cap-snatching endonuclease. (2019) Jones R, Lessoued S, Meier K, Devignot S, Barata-Garcia S, Mate M, Bragagnolo G, Weber F, Rosenthal M, Reguera J. Nucleic Acids Res 47 10914-10930 PMID:31584100
- The fungal ribonuclease-like effector protein CSEP0064/BEC1054 represses plant immunity and interferes with degradation of host ribosomal RNA. (2019) Pennington HG, Jones R, Kwon S, Bonciani G, Thieron H, Chandler T, Luong P, Morgan SN, Przydacz M, Bozkurt T, Bowden S, Craze M, Wallington EJ, Garnett J, Kwaaitaal M, Panstruga R, Cota E, Spanu PD. PLoS Pathog 15 e1007620 PMID:30856238
- Structure-based optimization of a PDZ-binding motif within a viral peptide stimulates neurite outgrowth. (2019) Khan Z, Terrien E, Delhommel F, Lefebvre-Omar C, Bohl D, Vitry S, Bernard C, Ramirez J, Chaffotte A, Ricquier K, Vincentelli R, Buc H, Prehaud C, Wolff N, Lafon M. J Biol Chem 294 13755-13768 PMID:31346033
Our team focuses on understanding viral infection mechanisms often ruled by the interplay of viral proteins, cellular proteins and nucleic acids assembled into multifunctional complexes. The aim is to understand the mechanisms by which the multiple enzymatic activities are coordinated between them and regulated to efficiently carry out infection. To reach our goals we use a broad range of biochemical and biophysical technics combined with X-ray crystallography and electron microscopy.
The mainstream of our research is the functional and structural characterization of the alphavirus replication complex. Alphavirus, like chikungunya virus (CHIK) or eastern equine encephalitis virus (EEEV), cause zoonotic infectious diseases with high impact in global human health. The viral genome replication and gene expression is carried out by the multifunctional viral replication complex. Interestingly this complex can adopt different functions depending on its state of maturation. Our goal is to structurally and functionally characterize the different functional states of the replication complex in order to propose an integrative mechanistic model accurately describing at the molecular level the viral infection cycle and its regulation. The results will provide invaluable insights and tools for the development of new strategies for the treatment of these concerning infectious diseases.
