{"id":8037,"date":"2023-09-20T15:30:16","date_gmt":"2023-09-20T14:30:16","guid":{"rendered":"https:\/\/www.afmb.univ-mrs.fr\/?post_type=event&#038;p=8037"},"modified":"2023-09-21T13:43:19","modified_gmt":"2023-09-21T12:43:19","slug":"regueras-team-guest-ylva-ivarsson-uppsala-university-sueden-large-scale-mapping-of-binding-sites-in-the-intrinsically-disordered-of-the-proteome","status":"publish","type":"event","link":"https:\/\/www.afmb.univ-mrs.fr\/en\/event\/regueras-team-guest-ylva-ivarsson-uppsala-university-sueden-large-scale-mapping-of-binding-sites-in-the-intrinsically-disordered-of-the-proteome\/","title":{"rendered":"J. Reguera&#8217;s team guest: Ylva Ivarsson, Uppsala University, Sweden &#8211; &#8220;Large-scale mapping of binding sites in the intrinsically disordered regions of the proteome&#8221;"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">Abstract<\/h2>\n\n\n\n<p>Numerous studies have provided large-scale information on human protein-protein interactions. However, many interactions remain to be discovered, and low affinity, conditional and cell type-specific interactions disproportionately under-represented. Most of the missing interactions are most likely mediated by short linear motifs (SLiMs). The SLiM-based interactions are of critical importance for cell function and regulation.&nbsp; Over the last ten years we have developed dedicated experimental methods for large-scale screening of SLiM-based interactions together with tools and guidelines for processing the generated data. I will describe our optimized proteomic peptide-phage display (ProP-PD) library that tiles all disordered regions of the human proteome and allows the screening of ~1,000,000 overlapping peptides in a single binding assay. Using this resource we have identified &gt;2,000 interaction SLiM-based interactions. The amino acid resolution binding site information can be used to pin-point functionally important disease mutations and phosphorylation events in intrinsically disordered regions of the proteome. &nbsp;Following this line, I will describe our novel study focused on uncovering how disease mutations make and break SLiM-based interactions. Finally, I will showcase how we employ ProP-PD to explore host-virus protein-protein interactions, and how this information can be used to identify peptides with antiviral activities.<\/p>\n","protected":false},"template":"","events_category":[15],"class_list":["post-8037","event","type-event","status-publish","hentry","events_category-seminar","entry"],"_links":{"self":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/event\/8037","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/event"}],"about":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/types\/event"}],"wp:attachment":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/media?parent=8037"}],"wp:term":[{"taxonomy":"events_category","embeddable":true,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/events_category?post=8037"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}