{"id":1060,"date":"2021-03-29T17:58:47","date_gmt":"2021-03-29T16:58:47","guid":{"rendered":"http:\/\/www.afmb.univ-mrs.fr\/?post_type=facility&#038;p=1060"},"modified":"2024-04-08T13:49:01","modified_gmt":"2024-04-08T12:49:01","slug":"structural-biology","status":"publish","type":"facility","link":"https:\/\/www.afmb.univ-mrs.fr\/en\/facility\/structural-biology\/","title":{"rendered":"Marseille Integrative Structural Biology Facility (PBSIM)"},"content":{"rendered":"\n<div class=\"wp-block-group alignfull pageSubHeaderWrapper topSection has-white-color has-dark-blue-background-color has-text-color has-background\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<ul class=\"wp-block-list\">\n<li><a href=\"#presentation\">Introduction<\/a><\/li>\n\n\n\n<li><a href=\"#services\">Services<\/a><\/li>\n<\/ul>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-columns presentationTeamWrapper is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\" id=\"presentation\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p class=\"has-large-font-size\">The Marseille integrative structural biology facility (PBSIM) coordinated by the AFMB lab gathers 6 services offering a state-of-the-art equipment and available to a broad scientific community from the academic and industrial fields.<\/p>\n\n\n\n<p>The facility offers a large panel of complementary techniques and expertise to tackle the tridimensional structures of proteins or macromolecular assemblies from synthetic genes and selected vectors\/plasmids. The facility represents one node of the French infrastructure for integrated structural biology (<a href=\"http:\/\/frisbi.eu\" target=\"_blank\" rel=\"noreferrer noopener\">FRISBI<\/a>) aimed at promoting technological transfer between industries and academic labs for technological developments and some therapeutic applications. It is also recognized by the <a href=\"https:\/\/www.ibisa.net\/plateformes\/plateforme-biologie-structurale-integree-marseille-pbsim-156.html\" target=\"_blank\" rel=\"noreferrer noopener\">IBiSA<\/a> and <a href=\"https:\/\/plateformes.univ-amu.fr\/\" target=\"_blank\" rel=\"noreferrer noopener\">Aix-Marseille<\/a> national labels.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column columnStickyWrapper is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<div class=\"wp-block-group stickyColumnGroup\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\"><div class=\"singleTeamLeaders clearfix\"><div class=\"singleTeamContactWrapper\"><h5>Team contacts<\/h5><ul><li><a href=\"https:\/\/frisbi.eu\/centers\/afmb\/\" target=\"_blank\"><i class=\"fas fa-link\"><\/i><\/a><\/li><li><a href=\"mailto:gerlind.sulzenbacher@univ-amu.fr\"><i class=\"far fa-envelope\"><\/i><\/a><\/li><li class=\"phoneIconWrapper\"><a class=\"phoneIconLink\"><i class=\"fas fa-tty\"><\/i><\/a><div class=\"phoneNumber\"><a href=\"tel:+33 (0)4 13 94 95 27\">+33 (0)4 13 94 95 27<\/a><\/div><\/li><\/ul><\/div><\/div>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div id=\"services\" class=\"wp-block-group subGroupsWrapper\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<h2 class=\"wp-block-heading\" id=\"services\">Services<\/h2>\n\n\n<div class=\"listOfTeamsWrapper clearfix\"><div class=\"row clearfix listOfTeams \"><div class=\"teamInListWrapper col-md-4\"><div class=\"teamInList fadeIn\">\n\n\n\t<a class=\"itemLink\" href=\"https:\/\/www.afmb.univ-mrs.fr\/en\/facility\/structural-biology\/high-throughput-cloning-expression-in-bacteria-purification-and-interaction-of-recombinant-proteins\/\" >\n            \n            \n                <div class=\"itemInfos\">\n                    \n                    <h4 class=\"itemTitle\">High-throughput protein production in E. coli, purification and interaction<\/h4>\n                                        <div class=\"groupLeaders\">\n                                                <div class=\"groupLeaderItem\">\n                            <div class=\"imgWrapper\">\n                                                                                                <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/DSC_0141-1-500x500.jpg\" width=\"500\" height=\"500\" alt=\"\" class=\"lazyload\" \/>\n                                                            <\/div>\n                            <h6 class=\"groupLeaderName\">Renaud Vincentelli<\/h6>\n                        <\/div>\n                                            <\/div>\n                                        <div class=\"groupExcerpt\"><p>Using its own high-throughput methods, this service performs all the necessary steps from cloning genes to purified proteins.<br \/>\nThe team develops strategies for its own research projects that are constantly evolving and enriching its service portfolio for its collaborators and clients.<\/p>\n<\/div>\n                <\/div>\n     \n            \n        <\/a>\n\n<\/div><!-- postInList -->\n<\/div><div class=\"teamInListWrapper col-md-4\"><div class=\"teamInList fadeIn\">\n\n\n\t<a class=\"itemLink\" href=\"https:\/\/www.afmb.univ-mrs.fr\/en\/facility\/structural-biology\/recombinant-protein-production-in-eukaryotic-systems\/\" >\n            \n            \n                <div class=\"itemInfos\">\n                    \n                    <h4 class=\"itemTitle\">Recombinant protein production in eukaryotic cell systems<\/h4>\n                                        <div class=\"groupLeaders\">\n                                                <div class=\"groupLeaderItem\">\n                            <div class=\"imgWrapper\">\n                                                                                                <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/Portrait-Claire-Debarnot-1-500x500.jpg\" width=\"500\" height=\"500\" alt=\"\" class=\"lazyload\" \/>\n                                                            <\/div>\n                            <h6 class=\"groupLeaderName\">Claire Debarnot<\/h6>\n                        <\/div>\n                                            <\/div>\n                                        <div class=\"groupExcerpt\"><p>This service offers the latest technologies for production of soluble, membrane-linked or membrane-embedded eukaryotic proteins from mammalian (HEK, CHO&#8230;) or insect (Sf9, S2&#8230;) cells.<\/p>\n<\/div>\n                <\/div>\n     \n            \n        <\/a>\n\n<\/div><!-- postInList -->\n<\/div><div class=\"teamInListWrapper col-md-4\"><div class=\"teamInList fadeIn\">\n\n\n\t<a class=\"itemLink\" href=\"https:\/\/www.afmb.univ-mrs.fr\/en\/facility\/structural-biology\/selection-and-production-of-camelid-vhhs-nanobodies\/\" >\n            \n            \n                <div class=\"itemInfos\">\n                    \n                    <h4 class=\"itemTitle\">Selection and production of camelid VHHs\/nanobodies<\/h4>\n                                        <div class=\"groupLeaders\">\n                                                <div class=\"groupLeaderItem\">\n                            <div class=\"imgWrapper\">\n                                                                                                <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/07\/Anais-Gaubert-scaled-e1655373789620-500x500.jpg\" width=\"500\" height=\"500\" alt=\"\" class=\"lazyload\" \/>\n                                                            <\/div>\n                            <h6 class=\"groupLeaderName\">Ana\u00efs Gaubert<\/h6>\n                        <\/div>\n                                            <\/div>\n                                        <div class=\"groupExcerpt\"><p>This service offers a set of expertises ranging from generation and selection of camelid nanobodies up to their production and functional characterization (SPR, BLI, micro-cal).<\/p>\n<\/div>\n                <\/div>\n     \n            \n        <\/a>\n\n<\/div><!-- postInList -->\n<\/div><div class=\"teamInListWrapper col-md-4\"><div class=\"teamInList fadeIn\">\n\n\n\t<a class=\"itemLink\" href=\"https:\/\/www.afmb.univ-mrs.fr\/en\/facility\/structural-biology\/biophysical-techniques\/\" >\n            \n            \n                <div class=\"itemInfos\">\n                    \n                    <h4 class=\"itemTitle\">Biophysical analysis of molecular interactions<\/h4>\n                                        <div class=\"groupLeaders\">\n                                                <div class=\"groupLeaderItem\">\n                            <div class=\"imgWrapper\">\n                                                                                                <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/DSC_0123-500x500.jpg\" width=\"500\" height=\"500\" alt=\"\" class=\"lazyload\" \/>\n                                                            <\/div>\n                            <h6 class=\"groupLeaderName\">Maria Mate<\/h6>\n                        <\/div>\n                                            <\/div>\n                                        <div class=\"groupExcerpt\"><p>This service offers a large panel of complementary equipment to  characterize molecular complexes and to screen or analyze molecular interactions, in solution or on solid support.<\/p>\n<\/div>\n                <\/div>\n     \n            \n        <\/a>\n\n<\/div><!-- postInList -->\n<\/div><div class=\"teamInListWrapper col-md-4\"><div class=\"teamInList fadeIn\">\n\n\n\t<a class=\"itemLink\" href=\"https:\/\/www.afmb.univ-mrs.fr\/en\/facility\/structural-biology\/crystallogenesis-and-crystallography\/\" >\n            \n            \n                <div class=\"itemInfos\">\n                    \n                    <h4 class=\"itemTitle\">Crystallization and crystallography<\/h4>\n                                        <div class=\"groupLeaders\">\n                                                <div class=\"groupLeaderItem\">\n                            <div class=\"imgWrapper\">\n                                                                                                <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/DSC_0237-scaled-e1646234356435-500x500.jpg\" width=\"500\" height=\"500\" alt=\"\" class=\"lazyload\" \/>\n                                                            <\/div>\n                            <h6 class=\"groupLeaderName\">Gerlind Sulzenbacher<\/h6>\n                        <\/div>\n                                                <div class=\"groupLeaderItem\">\n                            <div class=\"imgWrapper\">\n                                                                                                <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/Portrait-Veronique-Zamboni-1-500x500.jpg\" width=\"500\" height=\"500\" alt=\"\" class=\"lazyload\" \/>\n                                                            <\/div>\n                            <h6 class=\"groupLeaderName\">V\u00e9ronique Zamboni<\/h6>\n                        <\/div>\n                                            <\/div>\n                                        <div class=\"groupExcerpt\"><p>This service provides access to an automated infrastructure for the screening of hundreds of crystallization conditions using very small amounts of biological samples, along with regular observation of the crystallization trays.<\/p>\n<\/div>\n                <\/div>\n     \n            \n        <\/a>\n\n<\/div><!-- postInList -->\n<\/div><div class=\"teamInListWrapper col-md-4\"><div class=\"teamInList fadeIn\">\n\n\n\t<a class=\"itemLink\" href=\"https:\/\/www.afmb.univ-mrs.fr\/en\/facility\/structural-biology\/electron-microscopy\/\" >\n            \n            \n                <div class=\"itemInfos\">\n                    \n                    <h4 class=\"itemTitle\">Negative stain- and cryo-electron microscopy<\/h4>\n                                        <div class=\"groupLeaders\">\n                                                <div class=\"groupLeaderItem\">\n                            <div class=\"imgWrapper\">\n                                                                                                <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/02\/DSC_0148-scaled-e1646058900655-500x500.jpg\" width=\"500\" height=\"500\" alt=\"\" class=\"lazyload\" \/>\n                                                            <\/div>\n                            <h6 class=\"groupLeaderName\">Denis Ptchelkine<\/h6>\n                        <\/div>\n                                            <\/div>\n                                        <div class=\"groupExcerpt\"><p>This service offers the expertise for the structural characterization of biological systems and large macromolecular assemblies by negative-staining (Tecnai 12) and cryo (Glacios 2) electron microscopy.<\/p>\n<\/div>\n                <\/div>\n     \n            \n        <\/a>\n\n<\/div><!-- postInList -->\n<\/div><\/div><\/div>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>This facility gathers 6 services offering a large panel of complementary techniques and expertise to tackle the tridimensional structures of proteins or macromolecular assemblies from synthetic genes and selected vectors\/plasmids.<\/p>\n","protected":false},"author":1,"featured_media":722,"parent":0,"menu_order":1,"template":"","class_list":["post-1060","facility","type-facility","status-publish","has-post-thumbnail","hentry","entry"],"_links":{"self":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1060","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility"}],"about":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/types\/facility"}],"author":[{"embeddable":true,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/users\/1"}],"version-history":[{"count":20,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1060\/revisions"}],"predecessor-version":[{"id":9251,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1060\/revisions\/9251"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/media\/722"}],"wp:attachment":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/media?parent=1060"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}