{"id":1065,"date":"2021-03-29T18:01:08","date_gmt":"2021-03-29T17:01:08","guid":{"rendered":"http:\/\/www.afmb.univ-mrs.fr\/?post_type=facility&#038;p=1065"},"modified":"2025-11-20T13:42:42","modified_gmt":"2025-11-20T12:42:42","slug":"high-throughput-cloning-expression-in-bacteria-purification-and-interaction-of-recombinant-proteins","status":"publish","type":"facility","link":"https:\/\/www.afmb.univ-mrs.fr\/en\/facility\/structural-biology\/high-throughput-cloning-expression-in-bacteria-purification-and-interaction-of-recombinant-proteins\/","title":{"rendered":"High-throughput protein production in E. coli, purification and interaction"},"content":{"rendered":"\n<div class=\"wp-block-group alignfull pageSubHeaderWrapper topSection has-white-color has-dark-blue-background-color has-text-color has-background\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<ul class=\"wp-block-list\">\n<li><a href=\"#summary\" data-type=\"internal\" data-id=\"#summary\">Summary<\/a><\/li>\n\n\n\n<li><a href=\"#services\" data-type=\"internal\" data-id=\"#services\">Services<\/a><\/li>\n\n\n\n<li><a href=\"#devmet\" data-type=\"internal\" data-id=\"#devmet\">Development<\/a><\/li>\n\n\n\n<li><a href=\"#pjrecherche\" data-type=\"internal\" data-id=\"#pjrecherche\">Research<\/a><\/li>\n\n\n\n<li><a href=\"#networks\" data-type=\"internal\" data-id=\"#networks\">Network<\/a><\/li>\n\n\n\n<li><a href=\"#founding\" data-type=\"internal\" data-id=\"#founding\">Funding<\/a><\/li>\n\n\n\n<li><a href=\"#publi\" data-type=\"internal\" data-id=\"#publi\">Publications<\/a><\/li>\n\n\n\n<li><a href=\"#members\" data-type=\"internal\" data-id=\"#members\">Members<\/a><\/li>\n\n\n\n<li><a href=\"#news\" data-type=\"internal\" data-id=\"#news\">News<\/a><\/li>\n<\/ul>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-columns presentationTeamWrapper is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\" id=\"presentation\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p class=\"has-large-font-size\">This <a href=\"https:\/\/www.afmb.univ-mrs.fr\/en\/facility\/structural-biology\/\">PBSIM<\/a> facility uses its own high-throughput method to perform all the necessary steps from cloned genes to purified proteins. The team develops strategies for its own research projects that are constantly evolving and enriching its service portfolio for its collaborators and clients.<\/p>\n\n\n\n<p>The protein production and purification service is open to the comunity since 2004. It is recognized by several national labels: <a href=\"https:\/\/www.ibisa.net\/plateformes\/plateforme-biologie-structurale-integree-marseille-pbsim-156.html\" data-type=\"URL\" data-id=\"https:\/\/www.ibisa.net\/plateformes\/plateforme-biologie-structurale-integree-marseille-pbsim-156.html\" target=\"_blank\" rel=\"noreferrer noopener\">IBiSA<\/a>, <a href=\"https:\/\/plateformes.univ-amu.fr\/\" data-type=\"URL\" data-id=\"https:\/\/plateformes.univ-amu.fr\/\" target=\"_blank\" rel=\"noreferrer noopener\">AMU<\/a> and <a href=\"https:\/\/frisbi.eu\/centers\/afmb\/bacterial-expression\/\" data-type=\"URL\" data-id=\"https:\/\/frisbi.eu\/centers\/afmb\/bacterial-expression\/\" target=\"_blank\" rel=\"noreferrer noopener\">FRISBI<\/a>. It offers a portfolio of automated protocols (96 or 384 format) which cover all the stages of cloning, protein production and purification as well as the in vitro study of protein-protein, protein-peptide or protein-DNA interactions. Since its launch, more than 10,000 proteins have been produced for our customers, collaborators or partners from national (13 ANR as partner or subcontractor) or international (12 European networks) networks. The facility is co-author of more than eighty publications and regularly teaches some of its protocols in local (Polytech, Plinius), national (FRISBI, INSERM) and international (EMBO) practical training courses.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column columnStickyWrapper is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<div class=\"wp-block-group stickyColumnGroup\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\"><div class=\"singleTeamLeaders clearfix\"><div class=\"singleTeamContactWrapper\"><h5>Team contacts<\/h5><ul><li><a href=\"https:\/\/frisbi.eu\/centers\/afmb\/\" target=\"_blank\"><i class=\"fas fa-link\"><\/i><\/a><\/li><li><a href=\"mailto:renaud.vincentelli@univ-amu.fr\"><i class=\"far fa-envelope\"><\/i><\/a><\/li><li class=\"phoneIconWrapper\"><a class=\"phoneIconLink\"><i class=\"fas fa-tty\"><\/i><\/a><div class=\"phoneNumber\"><a href=\"tel:+33 (0)4 13 94 95 31\">+33 (0)4 13 94 95 31<\/a><\/div><\/li><\/ul><\/div><\/div>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"services\">Services<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Gene cloning<\/li>\n\n\n\n<li>Expression screening: optimization of <em>E. coli<\/em> culture conditions to improve protein solubility<\/li>\n\n\n\n<li>Protein refolding<\/li>\n\n\n\n<li>Purification<\/li>\n\n\n\n<li>In vitro Identification and quantification of protein-protein, protein-peptide (pull-down, holdup, Co-IP) or protein-DNA interactions (HT-SELEX)<\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-blue-20-color has-css-opacity has-blue-20-background-color has-background is-style-wide\"\/>\n\n\n\n<h5 class=\"wp-block-heading is-style-default has-orange-color has-text-color\" id=\"specific-equipment\">Specific Equipment<\/h5>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Two pipetting robots equipped with a 96-channel head EVO\u00ae(Tecan)<\/li>\n\n\n\n<li>One 96\/384 LabChip\u00ae GXTouch (Revvity) capillary electrophoresis system<\/li>\n\n\n\n<li>One Hydrospeed\u00ae 96\/384 plate-washer with vacuum or magnetic modules (Tecan)<\/li>\n\n\n\n<li>One Pherastar\u00ae FSX (BMG LabTech) plate-reader for 96\/384\/1536 samples  in UV\/Fluorescence\/Luminescence\/HTRF<\/li>\n\n\n\n<li>Two Microtron\u00ae (Infors) incubator for 48 DeepWells and 72 DW 24\/96 bacterial cultures respectively<\/li>\n\n\n\n<li>Four Akt\u00e4\u00aeXpress (Cytiva) purification systems (4&#215;4 modules) at 4\u00b0C<\/li>\n\n\n\n<li>One IDEAL96 magnetic beads purification (DNA and proteins) robot (ID solutions)<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"635\" height=\"379\" src=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/pipeline-2025.jpg\" alt=\"\" class=\"wp-image-11535\" style=\"width:840px;height:auto\" srcset=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/pipeline-2025.jpg 635w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/pipeline-2025-500x298.jpg 500w\" sizes=\"auto, (max-width: 635px) 100vw, 635px\" \/><\/figure>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-blue-20-color has-css-opacity has-blue-20-background-color has-background is-style-wide\"\/>\n\n\n\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"devmet\">Methods development<\/h2>\n\n\n\n<p>The vast majority of protocols used for the services have been developed, validated and published by the facility. We develop new protocols when necessary for our research projects or at the request of our customers. We collaborate and validate new methods with our colleagues from the <a href=\"https:\/\/p4eu.org\/\">P4EU<\/a> network (&gt;100 facilities) of which we are one of the five founding members (2011).<\/p>\n\n\n\n<p>All our analytical protocols are automated in microplate 96 or 384 format (Tecan robot) or on the AKTA Xpress (Cytiva) for large scale protein purification (mg).<\/p>\n\n\n\n<h5 class=\"wp-block-heading has-orange-color has-text-color\" id=\"expression-screening-optimization-of-culture-conditions-to-determine-and-improve-protein-solubility-level-in-e-coli\"><p id=\"tw-target-text\" class=\"tw-data-text tw-text-large tw-ta\" dir=\"ltr\" data-placeholder=\"Traduction\"><span class=\"Y2IQFc\" lang=\"en\">Expression screening: optimization of culture conditions to determine and improve protein solubility level in <em>E. coli<\/em><\/span><\/p> <em style=\"color: revert; font-size: revert; font-weight: revert;\">&nbsp;<\/em><\/h5>\n\n\n\n<figure class=\"wp-block-image size-large is-resized is-style-default\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"475\" src=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/figure11-1024x475.png\" alt=\"\" class=\"wp-image-2726\" style=\"width:835px;height:387px\" srcset=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/figure11-1024x475.png 1024w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/figure11-500x232.png 500w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/figure11-768x356.png 768w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/figure11-1536x713.png 1536w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/figure11.png 1920w\" sizes=\"auto, (max-width: 740px) 100vw, 740px\" \/><figcaption class=\"wp-element-caption\"><p id=\"tw-target-text\" class=\"tw-data-text tw-text-large tw-ta\" dir=\"ltr\" data-placeholder=\"Traduction\"><span class=\"Y2IQFc\" lang=\"en\">High-throughput expression screening: <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27165318\/\">Parameters that can alter protein solubility and quality<\/a><\/span><\/p><\/figcaption><\/figure>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-4-3 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"HTP Quantitative Expression Screening and Purification of proteins Produced in E. coli\" width=\"640\" height=\"480\" src=\"https:\/\/www.youtube.com\/embed\/MktApnHxxjw?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<p class=\"has-blue-70-color has-text-color\" style=\"font-size:12px\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/21925268\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Vincentelli et al. (2011) Methods<\/a>, <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23422067\/\" target=\"_blank\" rel=\"noreferrer noopener\">Vincentelli. R and Romier. C (2013) Curr Opin Struct Biol<\/a>, <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/24203323\/\" target=\"_blank\" rel=\"noreferrer noopener\">N. Saez and Vincentelli. R (2014) Methods Mol Biol.<\/a><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"513\" height=\"734\" src=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/Diapositive3-e1642417717608.jpeg\" alt=\"\" class=\"wp-image-3010\" style=\"width:400px;height:572px\" srcset=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/Diapositive3-e1642417717608.jpeg 513w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/Diapositive3-e1642417717608-349x500.jpeg 349w\" sizes=\"auto, (max-width: 513px) 100vw, 513px\" \/><figcaption class=\"wp-element-caption\">Maximum Throughput: 1152 expression screens per week<\/figcaption><\/figure>\n<\/div>\n<\/div>\n\n\n\n<h5 class=\"wp-block-heading has-orange-color has-text-color\" id=\"protein-renaturation\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/15388864\/\">Protein renaturation<\/a><\/h5>\n\n\n\n<h5 class=\"wp-block-heading has-orange-color has-text-color\" id=\"protein-purification\">Protein purification <\/h5>\n\n\n\n<p>The scale of production and purification is adapted according to the needs of the project:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><p id=\"tw-target-text\" class=\"tw-data-text tw-text-large tw-ta\" dir=\"ltr\" data-placeholder=\"Traduction\"><span class=\"Y2IQFc\" lang=\"en\">&#8220;Analytical&#8221; or &#8220;semi-preparative&#8221; culture and purification: culture in DeepWell 24\/96\/384 and purification on filter plates 24\/96\/384. This scale is used for &#8220;expression screening&#8221; or for the production of protein libraries. The largest library of purified proteins to date included <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28095880\/\">5,000 recombinant animal toxins<\/a>.<\/span><\/p><\/li>\n\n\n\n<li>Culture and &#8220;preparative&#8221; purification : culture in a 2-liter flask and purification on AKTA Xpress (Cytiva). All the  purification techniques are possible (affinity, gel-filtration, ion exchange, etc.). This scale is used for project containing one to a few tens of proteins at the milligram scale.<\/li>\n<\/ul>\n\n\n\n<h5 class=\"wp-block-heading has-orange-color has-text-color\" id=\"high-throughput-in-vitro-identification-and-quantification-of-protein-protein-protein-peptide-pull-down-holdup-co-ip-or-protein-dna-ht-selex-interactions\"><div id=\"tw-target-text-container\" class=\"tw-ta-container F0azHf tw-nfl\" tabindex=\"0\">\n<p id=\"tw-target-text\" class=\"tw-data-text tw-text-large tw-ta\" dir=\"ltr\" data-placeholder=\"Traduction\"><span class=\"Y2IQFc\" lang=\"en\">High-throughput <em>In vitro<\/em> identification and quantification of protein-protein, protein-peptide (pull-down, holdup, Co-IP) or protein-DNA (HT-SELEX) interactions.\n<\/span><\/p>\n\n<\/div>\n<div id=\"tw-target-rmn-container\" class=\"tw-target-rmn tw-ta-container F0azHf tw-nfl\">\n<p id=\"tw-target-rmn\" class=\"tw-data-placeholder tw-text-small tw-ta\" dir=\"ltr\"><\/p>\n\n<\/div><\/h5>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"720\" height=\"960\" src=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/holdup-1.jpeg\" alt=\"\" class=\"wp-image-2989\" style=\"width:452px;height:603px\" srcset=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/holdup-1.jpeg 720w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/holdup-1-375x500.jpeg 375w\" sizes=\"auto, (max-width: 720px) 100vw, 720px\" \/><figcaption class=\"wp-element-caption\">Figure 2: Principle, readout and data treatment of the high-throughput holdup assay.\n(See <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26053890\/\">Vincentelli et al. (2015) Nature Methods<\/a>)<\/figcaption><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"720\" height=\"960\" src=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/Diapositive1-1.jpeg\" alt=\"\" class=\"wp-image-2999\" style=\"width:450px;height:600px\" srcset=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/Diapositive1-1.jpeg 720w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/Diapositive1-1-375x500.jpeg 375w\" sizes=\"auto, (max-width: 720px) 100vw, 720px\" \/><figcaption class=\"wp-element-caption\">Schematic of the <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23332764\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">HT-SELEX<\/a> <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31267468\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">protocol<\/a><\/figcaption><\/figure>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"pjrecherche\">Research Projects and collaborations<\/h2>\n\n\n\n<p>The facility is involved as a partner, participant, or service provider (CRO) in research projects with private or public funds. Most of these projects require innovative protocols and this is the main driving force behind the evolution of the platform&#8217;s portfolio. The facility has participated in twelve European networks (FP5, FP6, FP7, H2020 funding) and sixteen ANRs (ANR is the French project research funding agency). Most of the projects stop with the end of funding, but some became long-term collaborations funded either by several ANRs (or successive European networks), or by our own funds.<\/p>\n\n\n\n<h5 class=\"wp-block-heading has-orange-color has-text-color\" id=\"animal-toxin-purification\">Animal toxin Purification<\/h5>\n\n\n\n<p id=\"tw-target-text\">Since 2010, the platform has developed various strategies to produce&nbsp;<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23607455\/\">animal toxins<\/a>&nbsp;in oxidized form in&nbsp;<em>E. coli<\/em>. These methods have been&nbsp;<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28093085\/\">refined<\/a>&nbsp;and applied on a&nbsp;<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28095880\/\">large scale<\/a>&nbsp;to thousands of toxins for the VENOMICS project (FP7, 2012-2015).<\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p>Since 2021, the facility has been one of the five European partners of the H2020&nbsp;<a href=\"https:\/\/addovenom.com\/\">Addovenom<\/a>&nbsp;project. This project seeks to develop a new treatment against snakebites which kill, even in the 21st century, more than 140,000 people every year. this project ended in early 2025.<br><br>For more informations about snakebites, you can see the documentary &#8220;Minutes To Die&#8221;<br><br>The facility is a member of the European COST&nbsp;<a href=\"https:\/\/euven-network.eu\/\">Euven<\/a>&nbsp;toxin network.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"Minutes to Die Documentary\" width=\"640\" height=\"360\" src=\"https:\/\/www.youtube.com\/embed\/jKOSo_9kvtA?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><figcaption class=\"wp-element-caption\">Minutes To Die Documentary<\/figcaption><\/figure>\n<\/div>\n<\/div>\n\n\n\n<h5 class=\"wp-block-heading has-orange-color has-text-color\" id=\"pdz-domains-purification-and-interaction\">PDZ domains purification and interaction<\/h5>\n\n\n\n<p>This project stems from a long collaboration between the facility and the team of Dr.&nbsp;<a href=\"https:\/\/www.igbmc.fr\/en\/recherche\/teams\/viral-oncoproteins-and-domain-motif-networks\">G. Trav\u00e9 at the IGBMC<\/a>&nbsp;which began with the ANR EPI-HPV-3D (2007-2010). This collaboration is still very active.<\/p>\n\n\n\n<p><span class=\"Y2IQFc\" lang=\"en\">We have developed and improved together a <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31267466\/\">library of clones, a protocol for the production of human PDZ domains<\/a> as well as a high-throughput protocol for the identification and quantification of <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26053890\/\">PDZ-PBM interactions<\/a> that can now determine <a href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2021.10.22.465449v1\">thousands affinities\/day<\/a> and is applicable to a very large number of protein-protein or protein-peptide interactions. The PDZ libraries and these methods have been used by several European teams, in particular by <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33502198\/\">members<\/a> of the ITN H2020 PDZNet project, whose facility was one of the associated laboratories.<\/span><\/p>\n\n\n\n<h5 class=\"wp-block-heading has-orange-color has-text-color\" id=\"cazymes-purification\">CAZYmes purification<\/h5>\n\n\n\n<p>The facility has produced and purified more than 2.000&nbsp;<a href=\"http:\/\/www.cazy.org\/\">CAZYmes<\/a>&nbsp;for the&nbsp;<a href=\"https:\/\/www.afmb.univ-mrs.fr\/en\/team\/glycogenomics\/\">Glycogenomics<\/a>&nbsp;team (Bernard Henrissat and Nicolas Terrapon) and its collaborators, mainly within the framework of three successive ANRs (<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/30850540\/\">1000 CAZymes<\/a>, Pulmarin and ODE) and the NovoNordisk foundation CAZAI project.<\/p>\n\n\n\n<h5 class=\"wp-block-heading has-orange-color has-text-color\" id=\"current-grants\">Current grants<\/h5>\n\n\n\n<p>Coordinator&nbsp;: <\/p>\n\n\n\n<p>ANR Full-PDZ-PBM-HPVE6 (2023-2025). Partner&nbsp;: Dr.&nbsp;<a href=\"https:\/\/www.igbmc.fr\/en\/recherche\/teams\/viral-oncoproteins-and-domain-motif-networks\">G. Trav\u00e9 at the IGBMC<\/a>.<\/p>\n\n\n\n<p>Partner&nbsp;:<\/p>\n\n\n\n<p>European&nbsp;: <a href=\"http:\/\/addovenom.com\">Addovenom<\/a> (2021-2025)<\/p>\n\n\n\n<p>NovoNordisk foundation : CAZAI (2023-2027)<\/p>\n\n\n\n<p>ANR AirMN (2021-2025)<\/p>\n\n\n\n<p>Participant : <\/p>\n\n\n\n<p>ANR Rha (2024-2027) and ODE (2021-2024)<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"networks\">Collaboration and services<\/h2>\n\n\n\n<p>The collaborations and services mentioned below correspond to the period 2016-2021. When a link exists, it refers to the last common publication over the period.\n\n<\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h5 class=\"wp-block-heading has-text-align-center has-orange-color has-text-color\" id=\"national\">National<\/h5>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<ul class=\"wp-block-list\">\n<li><strong>Marie-No\u00eblle Rosso, Giuliano Sciara<\/strong>\n<ul class=\"wp-block-list\">\n<li>BBF, Marseille<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>R\u00e9mi Lasserre, Phillipe Naquet, Beatrice NalRogier, Philippe Pierre<\/strong>\n<ul class=\"wp-block-list\">\n<li>CIML, Marseille<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31267466\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Jean-Paul Borg,<\/a> <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31267466\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Patrick Fourquet, <\/a><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33502198\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\" data-id=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33502198\/\">Pascale Zimmermann<\/a><\/strong>\n<ul class=\"wp-block-list\">\n<li>CRCM, Marseille<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34350965\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\" data-id=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34350965\/\">Manos Mavrakis<\/a>, <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27328749\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">J\u00e9rome Wenger<\/a><\/strong>\n<ul class=\"wp-block-list\">\n<li>Fresnel Institute, Marseille<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>Andr\u00e9 Le Bivic, Laurent Kodjabachian, <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31181075\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Alph\u00e9e Michelot<\/a>, Aziz Mogrich, Thomas Rival<\/strong>\n<ul class=\"wp-block-list\">\n<li>IBDML, Marseille<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>F\u00e9lix Rico, Olivier Th\u00e9odely<\/strong>\n<ul class=\"wp-block-list\">\n<li>LAI, Marseille<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/29076618\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Fran\u00e7ois Alberto<\/a>, <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/30013238\/\" data-type=\"URL\">T\u00e2m Mignot<\/a><\/strong>\n<ul class=\"wp-block-list\">\n<li>LCB, Marseille<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>Andreas Zanzoni, Christine Brun<\/strong>\n<ul class=\"wp-block-list\">\n<li>TAGC, Marseille<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34697603\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Bruno Coutard<\/a><\/strong>\n<ul class=\"wp-block-list\">\n<li>UVE, Marseille<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<ul class=\"wp-block-list\">\n<li><strong>J\u00e9rome Martin, Fran\u00e7ois Torney<\/strong>\n<ul class=\"wp-block-list\">\n<li>Biogemma\/Limagrain, Chappes<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32602722\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Nicolas Gilles<\/a><\/strong>\n<ul class=\"wp-block-list\">\n<li>CEA, Saclay<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/30850540\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">William Helbert<\/a><\/strong>\n<ul class=\"wp-block-list\">\n<li>Cermav, Grenoble<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/29149270\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Patrick Lemaire<\/a><\/strong>\n<ul class=\"wp-block-list\">\n<li>CRBM, Montpellier<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34350965\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">St\u00e9phanie Cabantous<\/a><\/strong>\n<ul class=\"wp-block-list\">\n<li>CRCT, Toulouse<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33781758\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Christophe Romier<\/a>, <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33781758\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Arnaud Poterszman<\/a>, <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/30726710\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Gilles Trav\u00e9<\/a><\/strong>\n<ul class=\"wp-block-list\">\n<li>IGBMC, Strasbourg<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/29089430\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Pascale Cossart<\/a>, <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33864728\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Nicolas Wolff<\/a><\/strong>\n<ul class=\"wp-block-list\">\n<li>Pasteur Institute, Paris<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>G\u00e9rard Lambeau<\/strong>\n<ul class=\"wp-block-list\">\n<li>IPMC, Valbonne<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h5 class=\"wp-block-heading has-text-align-center has-orange-color has-text-color\" id=\"international\">International<\/h5>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<ul class=\"wp-block-list\">\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33781758\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Kim Remans<\/a><\/strong>\n<ul class=\"wp-block-list\">\n<li>EMBL, Heidelberg, Germany<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31267452\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Carlos Fontes<\/a><\/strong>\n<ul class=\"wp-block-list\">\n<li>NZYTech, Lisbon, Portugal<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>Cherith Reid<\/strong>\n<ul class=\"wp-block-list\">\n<li>Randox, Crumlin, UK<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>Christiane Berger-Schaffitzel, Imre Berger<\/strong>\n<ul class=\"wp-block-list\">\n<li>University of Bristol, UK<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34210959\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Henrick Clausen,<\/a> <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33502198\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Kristian Str\u00f8mgaard<\/a><\/strong>\n<ul class=\"wp-block-list\">\n<li>University of Coppenhagen, Denmark<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/30204278\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Douwe van Sinderen<\/a><\/strong>\n<ul class=\"wp-block-list\">\n<li>University College Cork, Ireland<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32294469\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">L\u00e1szl\u00f3 Nyitray<\/a><\/strong>\n<ul class=\"wp-block-list\">\n<li>University E\u00f6tv\u00f6s Lor\u00e1nd, Budapest, Hungary<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32602722\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Edwin De Pauw,<\/a> <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28095880\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Loic Quinton<\/a><\/strong>\n<ul class=\"wp-block-list\">\n<li>Universit\u00e9 de Li\u00e8ge, Belgium<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>Stephane Mesnage<\/strong>\n<ul class=\"wp-block-list\">\n<li>University of Sheffield, UK<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>Jonas V. Schaefer<\/strong>\n<ul class=\"wp-block-list\">\n<li>University of Zurich, Switzerland<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>Johnny Habchi<\/strong>\n<ul class=\"wp-block-list\">\n<li>Wren Therapeutics Ltd, Cambridge, UK<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<ul class=\"wp-block-list\">\n<li><strong>Robert Allan Heinzen<\/strong>\n<ul class=\"wp-block-list\">\n<li>NIH Rocky Mountain, Montana, USA<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33781758\/\" target=\"_blank\" rel=\"noreferrer noopener\" data-type=\"URL\">Sarel-Jacob Fleishman<\/a><\/strong>\n<ul class=\"wp-block-list\">\n<li>Weizmann Institute, Rehovot, Isra\u00ebl<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"720\" height=\"405\" src=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/Diapositive1-4.jpeg\" alt=\"\" class=\"wp-image-3448\" srcset=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/Diapositive1-4.jpeg 720w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/Diapositive1-4-500x280.jpeg 500w\" sizes=\"auto, (max-width: 720px) 100vw, 720px\" \/><\/figure>\n\n\n\n<div class=\"wp-block-columns tableau is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div id=\"network\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<h5 class=\"wp-block-heading is-style-default has-orange-color has-text-color\" id=\"access\">Access<\/h5>\n\n\n\n<p>Academic and private companies<\/p>\n<\/div><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div id=\"funding\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<h5 class=\"wp-block-heading is-style-default has-orange-color has-text-color\" id=\"cost\">Cost<\/h5>\n\n\n\n<p>Coming soon<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"founding\">Fundings<\/h2>\n\n\n\n<div class=\"wp-block-group alignwide\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<figure class=\"wp-block-gallery has-nested-images columns-4 wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"576\" data-id=\"2229\" src=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/R-1-1024x576.png\" alt=\"\" class=\"wp-image-2229\" srcset=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/R-1-1024x576.png 1024w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/R-1-500x280.png 500w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/R-1-768x432.png 768w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/R-1-1536x864.png 1536w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/R-1.png 1920w\" sizes=\"auto, (max-width: 740px) 100vw, 740px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" data-id=\"2219\" src=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/logo-amu.png\" alt=\"\" class=\"wp-image-2219\"\/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"389\" data-id=\"2235\" src=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/Horizon2020-header-1024x389.jpg\" alt=\"\" class=\"wp-image-2235\" srcset=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/Horizon2020-header-1024x389.jpg 1024w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/Horizon2020-header-500x190.jpg 500w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/Horizon2020-header-768x292.jpg 768w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/Horizon2020-header-1536x584.jpg 1536w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/Horizon2020-header.jpg 1730w\" sizes=\"auto, (max-width: 740px) 100vw, 740px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" data-id=\"2237\" src=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/i0Dzu1O_400x400.jpg\" alt=\"\" class=\"wp-image-2237\"\/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"897\" height=\"167\" data-id=\"2217\" src=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/logo_ibisa.png\" alt=\"\" class=\"wp-image-2217\" srcset=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/logo_ibisa.png 897w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/logo_ibisa-500x93.png 500w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/logo_ibisa-768x143.png 768w\" sizes=\"auto, (max-width: 897px) 100vw, 897px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"273\" data-id=\"2215\" src=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/logo_amidex-1024x273.jpg\" alt=\"\" class=\"wp-image-2215\" srcset=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/logo_amidex-1024x273.jpg 1024w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/logo_amidex-500x133.jpg 500w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/logo_amidex-768x205.jpg 768w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/logo_amidex-1536x409.jpg 1536w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/logo_amidex-2048x546.jpg 2048w\" sizes=\"auto, (max-width: 740px) 100vw, 740px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"471\" height=\"133\" data-id=\"2221\" src=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/logo-CENTURI-horizontal-azur.png\" alt=\"\" class=\"wp-image-2221\"\/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" data-id=\"2225\" src=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/marie_curie_logo.png\" alt=\"\" class=\"wp-image-2225\"\/><\/figure>\n<\/figure>\n<\/div><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"publi\">Publication<\/h2>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p><p id=\"tw-target-text\" class=\"tw-data-text tw-text-large tw-ta\" dir=\"ltr\" data-placeholder=\"Traduction\"><span class=\"Y2IQFc\" lang=\"en\">The development of methods and the work carried out on the facility for our collaborators has given rise to more than sixty publications as co-authors, including articles in journals with high impact factors such as: Cell, Nature Methods, Nature Microbiology, Nature Communication, PNAS. The team also wrote five book chapters and coordinated the editing of the first book in the &#8220;Methods in Molecular Biology&#8221; series dedicated to &#8220;<a href=\"https:\/\/link.springer.com\/book\/10.1007\/978-1-4939-9624-7\">High-Throughput Protein Production and Purification<\/a>&#8221; (2019).<\/span><\/p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"721\" height=\"1024\" src=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/2025-1-721x1024.jpg\" alt=\"\" class=\"wp-image-2746\" style=\"width:93px;height:131px\" srcset=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/2025-1-721x1024.jpg 721w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/2025-1-352x500.jpg 352w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/2025-1-768x1091.jpg 768w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/01\/2025-1.jpg 827w\" sizes=\"auto, (max-width: 721px) 100vw, 721px\" \/><\/figure>\n<\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"members\">Members<\/h2>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><div class=\"lazyblock-member-block-Z2lQXvI wp-block-lazyblock-member-block\"><div class=\"memberInList fadeIn\">\n    <a class=\"itemLink\" href=\"https:\/\/www.afmb.univ-mrs.fr\/en\/member\/renaud-vincentelli\/\">\n        <div class=\"imgWrapper\">\n                                    <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/DSC_0141-1-500x500.jpg\" width=\"500\" height=\"500\" alt=\"\" class=\"lazyload\" \/>\n                    <\/div>\n        <div class=\"memberInfos\">\n            <p class=\"memberInListName\">Renaud Vincentelli<\/p>\n                        <div class=\"memberInListExcerpt\">\n            <p>Research engineer<\/p>\n            <\/div>\n                                <\/div>\n    <\/a>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"news\">News<\/h2>\n\n\n\n<h3 class=\"wp-block-heading has-dark-blue-color has-text-color\" id=\"october-5-2021-first-addovenom-annual-meeting-kicks-off-in-bristol\"><strong>September 13, 2022<\/strong> ADDovenom Year 2 annual meeting in Marseille<\/h3>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"653\" src=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/09\/IMG_9425-1024x653.jpg\" alt=\"\" class=\"wp-image-4950\" style=\"width:580px;height:369px\" srcset=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/09\/IMG_9425-1024x653.jpg 1024w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/09\/IMG_9425-500x319.jpg 500w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/09\/IMG_9425-768x490.jpg 768w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/09\/IMG_9425-1536x979.jpg 1536w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/09\/IMG_9425-2048x1306.jpg 2048w\" sizes=\"auto, (max-width: 740px) 100vw, 740px\" \/><\/figure>\n\n\n\n<h3 class=\"wp-block-heading has-dark-blue-color has-text-color\" id=\"october-5-2021-first-addovenom-annual-meeting-kicks-off-in-bristol\"><strong>October 5, 2021<\/strong>\n<a href=\"https:\/\/addovenom.com\/first-addovenom-annual-meeting-kicks-off-in-bristol\/\" data-type=\"URL\" data-id=\"https:\/\/addovenom.com\/first-addovenom-annual-meeting-kicks-off-in-bristol\/\">First ADDovenom annual meeting kicks off in Bristol<\/a><\/h3>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"687\" src=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/09\/20210927_135353-scaled-1-1024x687.jpg\" alt=\"\" class=\"wp-image-4952\" style=\"width:579px;height:388px\" srcset=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/09\/20210927_135353-scaled-1-1024x687.jpg 1024w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/09\/20210927_135353-scaled-1-500x336.jpg 500w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/09\/20210927_135353-scaled-1-768x516.jpg 768w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/09\/20210927_135353-scaled-1-1536x1031.jpg 1536w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2022\/09\/20210927_135353-scaled-1.jpg 1920w\" sizes=\"auto, (max-width: 740px) 100vw, 740px\" \/><\/figure>\n\n\n\n<h3 class=\"wp-block-heading has-dark-blue-color has-text-color\" id=\"january-7-2021-la-provence-the-most-read-newspaper-in-the-south-of-france-writes-about-our-addovenom-research\"><strong>January 7, 2021 <\/strong><a href=\"https:\/\/addovenom.com\/la-provence-the-most-read-newspaper-in-the-south-of-france-writes-about-our-addovenom-research\/\" data-type=\"URL\" data-id=\"https:\/\/addovenom.com\/la-provence-the-most-read-newspaper-in-the-south-of-france-writes-about-our-addovenom-research\/\">La Provence, the most read newspaper in the south of France, writes about our ADDovenom research<\/a><\/h3>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Using its own high-throughput methods, this service performs all the necessary steps from cloning genes to purified proteins.<br \/>\nThe team develops strategies for its own research projects that are constantly evolving and enriching its service portfolio for its collaborators and clients.<\/p>\n","protected":false},"author":1,"featured_media":718,"parent":1060,"menu_order":1,"template":"","class_list":["post-1065","facility","type-facility","status-publish","has-post-thumbnail","hentry","entry"],"_links":{"self":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1065","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility"}],"about":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/types\/facility"}],"author":[{"embeddable":true,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/users\/1"}],"version-history":[{"count":75,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1065\/revisions"}],"predecessor-version":[{"id":11542,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1065\/revisions\/11542"}],"up":[{"embeddable":true,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1060"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/media\/718"}],"wp:attachment":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/media?parent=1065"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}