{"id":1067,"date":"2021-03-29T18:04:32","date_gmt":"2021-03-29T17:04:32","guid":{"rendered":"http:\/\/www.afmb.univ-mrs.fr\/?post_type=facility&#038;p=1067"},"modified":"2026-01-05T13:21:24","modified_gmt":"2026-01-05T12:21:24","slug":"recombinant-protein-production-in-eukaryotic-systems","status":"publish","type":"facility","link":"https:\/\/www.afmb.univ-mrs.fr\/en\/facility\/structural-biology\/recombinant-protein-production-in-eukaryotic-systems\/","title":{"rendered":"Recombinant protein production in eukaryotic cell systems"},"content":{"rendered":"\n<div class=\"wp-block-group alignfull pageSubHeaderWrapper topSection has-white-color has-dark-blue-background-color has-text-color has-background\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<ul class=\"wp-block-list\">\n<li><a href=\"#presentation\">Introduction<\/a><\/li>\n<\/ul>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-columns presentationTeamWrapper is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\" id=\"presentation\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p class=\"has-large-font-size\">This <a href=\"https:\/\/www.afmb.univ-mrs.fr\/en\/facility\/structural-biology\/\">PBSIM<\/a> service offers the latest technologies for production of soluble eukaryotic proteins in mammalian (HEK293, CHO, COS-7) or insect cells (S2, Sf9), which are able to process most of the post-translational modifications (disulfide bridges, N- and O-glycosylation, phosphorylation\u2026) critical for protein structure or function.<\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<p>The feasibility study associates the specification needs of the applicant (protein amounts, tags for purification and detection\u2026) and the expertise of the facility staff. <\/p>\n\n\n\n<p>The selected protocol is assayed, validated and then amplified for a quantitative production assay. The produced proteins are purified and characterized by the applicants.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/04\/cultureC-59cfe.jpg\" alt=\"\" class=\"wp-image-603\"\/><\/figure>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns tableau is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div id=\"network\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<h6 class=\"wp-block-heading is-style-default has-orange-color has-text-color\" id=\"accessibility\">Accessibility<\/h6>\n\n\n\n<p>Academia and industries<\/p>\n<\/div><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div id=\"funding\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<h6 class=\"wp-block-heading is-style-default has-orange-color has-text-color\" id=\"cost\">Cost<\/h6>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Phase 1<\/strong> (evaluation)&nbsp;: <br>based on the system used.<\/li>\n\n\n\n<li><strong>Phase 2<\/strong> (production)&nbsp;: <br>based on the expression yields and protein amounts required.<\/li>\n<\/ul>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns tableau is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div id=\"network\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<h6 class=\"wp-block-heading is-style-default has-orange-color has-text-color\" id=\"how-to-apply\">How to apply?<\/h6>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>AFMB Contact:<\/strong> <a href=\"https:\/\/www.afmb.univ-mrs.fr\/en\/member\/claire-debarnot\/\" data-type=\"member\" data-id=\"960\">Claire Debarnot<\/a><\/li>\n<\/ul>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns tableau is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div id=\"network\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<h6 class=\"wp-block-heading is-style-default has-orange-color has-text-color\" id=\"dedicated-equipment\">Dedicated equipment<\/h6>\n\n\n\n<ul class=\"wp-block-list\">\n<li>4 CO2 incubators<\/li>\n\n\n\n<li>3 temperature-controlled incubators<\/li>\n\n\n\n<li>4 laminar flow hoods<\/li>\n\n\n\n<li>1 epifluorescence microscope<\/li>\n\n\n\n<li>1 inverted microscope<\/li>\n<\/ul>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column columnStickyWrapper is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<div class=\"wp-block-group stickyColumnGroup\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\"><div class=\"singleTeamLeaders clearfix\"><div class=\"singleTeamContactWrapper\"><h5>Team contacts<\/h5><ul><li><a href=\"https:\/\/frisbi.eu\/centers\/afmb\/\" target=\"_blank\"><i class=\"fas fa-link\"><\/i><\/a><\/li><li><a href=\"mailto:claire.debarnot@univ-amu.fr\"><i class=\"far fa-envelope\"><\/i><\/a><\/li><li class=\"phoneIconWrapper\"><a class=\"phoneIconLink\"><i class=\"fas fa-tty\"><\/i><\/a><div class=\"phoneNumber\"><a href=\"tel:+33 (0)4 13 94 95 03\">+33 (0)4 13 94 95 03<\/a><\/div><\/li><\/ul><\/div><\/div>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Members<\/h2>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><div class=\"lazyblock-member-block-1XsUud wp-block-lazyblock-member-block\"><div class=\"memberInList fadeIn\">\n    <a class=\"itemLink\" href=\"https:\/\/www.afmb.univ-mrs.fr\/en\/member\/claire-debarnot\/\">\n        <div class=\"imgWrapper\">\n                                    <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/03\/Portrait-Claire-Debarnot-1-500x500.jpg\" width=\"500\" height=\"500\" alt=\"\" class=\"lazyload\" \/>\n                    <\/div>\n        <div class=\"memberInfos\">\n            <p class=\"memberInListName\">Claire Debarnot<\/p>\n                        <div class=\"memberInListExcerpt\">\n            <p>Engineer<br \/>\nAMU<\/p>\n            <\/div>\n                                <\/div>\n    <\/a>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><div class=\"lazyblock-member-block-OyplQ wp-block-lazyblock-member-block\"><div class=\"memberInList fadeIn\">\n    <a class=\"itemLink\" href=\"https:\/\/www.afmb.univ-mrs.fr\/en\/member\/malorie-spies\/\">\n        <div class=\"imgWrapper\">\n                        <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/themes\/afmb\/assets\/img\/no-image-user.png\" alt=\"\" width=\"50\" height=\"50\" \/>\n                    <\/div>\n        <div class=\"memberInfos\">\n            <p class=\"memberInListName\">Malorie Spies<\/p>\n                        <div class=\"memberInListExcerpt\">\n            <p>Master-2 student<br \/>\nIM2B facilities<\/p>\n            <\/div>\n                                <\/div>\n    <\/a>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-group accordeon\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<h4 class=\"wp-block-heading accordeonTitle\" id=\"publications-linked-to-the-techniques-used\">Publications related to the techniques used<\/h4>\n\n\n\n<div class=\"wp-block-group accordeonContent\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<ul class=\"wp-block-list\">\n<li>Aricescu AR, Lu W, Jones EY (2006) A time- and cost-efficient system for high-level protein production in mammalian cells. <strong>Acta Cryst<\/strong> D62, 1243\u20131250.<\/li>\n\n\n\n<li>Durocher Y, Loignon M, Process, vectors and engineered cell lines for enhanced large-scale transfection, <strong>National Research Council of Canada<\/strong>, WO2009\/137911 (patent).<\/li>\n\n\n\n<li>Liepman AH, Wilkerson CG, Keegstra K (2005) Expression of cellulose synthase-like (Csl) genes in insect cells reveals that CslA family members encode mannan synthases. <strong>Proc Natl Acad Sci USA<\/strong> 102:2221-2226.<\/li>\n\n\n\n<li>Yao F, Svensj\u00f6 T, Winkler T, Lu M, Eriksson C, Eriksson E (1998) Tetracycline repressor, tetR, rather than the tetR-mammalian cell transcription factor fusion derivatives, regulates inducible gene expression in mammalian cells. <strong>Hum Gene Ther<\/strong> 9, 1939-1950.<\/li>\n<\/ul>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>This service offers the latest technologies for production of soluble, membrane-linked or membrane-embedded eukaryotic proteins from mammalian (HEK, CHO&#8230;) or insect (Sf9, S2&#8230;) cells.<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":1060,"menu_order":2,"template":"","class_list":["post-1067","facility","type-facility","status-publish","hentry","entry"],"_links":{"self":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1067","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility"}],"about":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/types\/facility"}],"author":[{"embeddable":true,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/users\/1"}],"version-history":[{"count":22,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1067\/revisions"}],"predecessor-version":[{"id":11655,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1067\/revisions\/11655"}],"up":[{"embeddable":true,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1060"}],"wp:attachment":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/media?parent=1067"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}