{"id":1070,"date":"2021-03-29T18:05:02","date_gmt":"2021-03-29T17:05:02","guid":{"rendered":"http:\/\/www.afmb.univ-mrs.fr\/?post_type=facility&#038;p=1070"},"modified":"2026-03-17T15:40:45","modified_gmt":"2026-03-17T14:40:45","slug":"biophysical-techniques","status":"publish","type":"facility","link":"https:\/\/www.afmb.univ-mrs.fr\/en\/facility\/structural-biology\/biophysical-techniques\/","title":{"rendered":"Biophysical analysis of molecular interactions"},"content":{"rendered":"\n<div class=\"wp-block-group alignfull pageSubHeaderWrapper topSection has-white-color has-dark-blue-background-color has-text-color has-background\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<ul class=\"wp-block-list\">\n<li><a href=\"#presentation\">Introduction<\/a><\/li>\n<\/ul>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-columns presentationTeamWrapper is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\" id=\"presentation\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p class=\"has-large-font-size\">This <a href=\"https:\/\/www.afmb.univ-mrs.fr\/en\/facility\/structural-biology\/\">PBSIM<\/a> service offers a large panel of state-of-the-art equipment for the characterization of molecular complexes and the screening or analysis of molecular interactions, in solution or on solid support.<\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<p>These tools are particularly adapted to the study of various complexes such as:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>protein\/protein<\/li>\n\n\n\n<li>protein\/nucleic acid<\/li>\n\n\n\n<li>protein\/small molecule<\/li>\n\n\n\n<li>protein\/detergent<\/li>\n<\/ul>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-large\"><a href=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/04\/IMG_2843-84953.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"450\" height=\"450\" src=\"http:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/04\/IMG_2843-84953.jpg\" alt=\"\" class=\"wp-image-615\" srcset=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/04\/IMG_2843-84953.jpg 450w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/04\/IMG_2843-84953-300x300.jpg 300w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2021\/04\/IMG_2843-84953-150x150.jpg 150w\" sizes=\"auto, (max-width: 450px) 100vw, 450px\" \/><\/a><\/figure>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns tableau is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div id=\"network\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<h6 class=\"wp-block-heading is-style-default has-orange-color has-text-color\" id=\"accessibility\">Accessibility<\/h6>\n\n\n\n<p>Academia and industries<\/p>\n<\/div><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div id=\"funding\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<h6 class=\"wp-block-heading is-style-default has-orange-color has-text-color\" id=\"cost\">Cost<\/h6>\n\n\n\n<p>To be defined from the service requested.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns tableau is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div id=\"network\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<h6 class=\"wp-block-heading is-style-default has-orange-color has-text-color\" id=\"how-to-access\">How to access ?<\/h6>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a rel=\"noreferrer noopener\" href=\"http:\/\/frisbi.eu\/submit-a-proposal\/\" target=\"_blank\">Complete the proposal from<\/a><br>and check \u201cMarseille\u201d from the \u201cBiophysical facility\u201d item<\/li>\n\n\n\n<li><strong>Contact at AFMB&nbsp;:<\/strong> <a href=\"https:\/\/www.afmb.univ-mrs.fr\/en\/member\/maria-mate\/\" data-type=\"member\" data-id=\"994\">Maria Mate<\/a><\/li>\n<\/ul>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns tableau is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div id=\"network\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<h6 class=\"wp-block-heading is-style-default has-orange-color has-text-color\" id=\"specific-equipement\">Specific equipement<\/h6>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Isothermal titration calorimetry (ITC): MicroCal iTC200 (Malvern Panalytical)<\/li>\n\n\n\n<li>Microscale thermophoresis (MST): Monolith NT.115 (NanoTemper Technologies GmbH)<\/li>\n\n\n\n<li>Size exclusion chromatography\/multi-angle light scattering\/ refractometry (SEC-MALS) :<\/li>\n\n\n\n<li>HPLC Ultimate 3000 (Thermo Scientific)\/ Dawn 8 (Wyatt)\/ Optilab (Wyatt)<\/li>\n\n\n\n<li>Bio-layer interferometry (BLI) : Octet RED96 et Blitz (ForteBio, Molecular Devices)<\/li>\n\n\n\n<li>Fluorescence-detection size exclusion chromatography (FSEC): HPLC Dionex\/ Fluorescence detector (Thermo Scientific)<\/li>\n\n\n\n<li>Nano differential scanning fluorimetry (nanoDSF): Tycho (NanoTemper Technologies GmbH)<\/li>\n\n\n\n<li>Dynamic light scattering (DLS): Zetasizer (Malvern Panalytical)<\/li>\n\n\n\n<li>Circular dichroism (CD): J-810 (Jasco)<\/li>\n<\/ul>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column columnStickyWrapper is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<div class=\"wp-block-group stickyColumnGroup\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\"><div class=\"singleTeamLeaders clearfix\"><div class=\"singleTeamContactWrapper\"><h5>Team contacts<\/h5><ul><li><a href=\"https:\/\/frisbi.eu\/centers\/afmb\/\" target=\"_blank\"><i class=\"fas fa-link\"><\/i><\/a><\/li><li><a href=\"mailto:maria.mate@univ-amu.fr\"><i class=\"far fa-envelope\"><\/i><\/a><\/li><li class=\"phoneIconWrapper\"><a class=\"phoneIconLink\"><i class=\"fas fa-tty\"><\/i><\/a><div class=\"phoneNumber\"><a href=\"tel:+33 (0)4 13 94 95 19\">+33 (0)4 13 94 95 19<\/a><\/div><\/li><\/ul><\/div><\/div>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-group accordeon\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<h4 class=\"wp-block-heading accordeonTitle\" id=\"publications\">Publications<\/h4>\n\n\n\n<div class=\"wp-block-group accordeonContent\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<ul class=\"wp-block-list\">\n<li>Broc M, et al (2025) A scaffold for quinone channeling between membrane and soluble bacterial oxidoreductases. Nat Struct Mol Biol 32(11):2196-2202.<\/li>\n\n\n\n<li>Fosses A, et al (2023) Role of the solute-binding protein CuaD in the signaling and regulating pathway of cellobiose and cellulose utilization in <em>Ruminiclostridium cellulolyticum<\/em>. Microorganism<strong>s<\/strong> 11(7):1732.<\/li>\n\n\n\n<li>Ortega Granda O, et al (2023) Macro1 domain residue F156: A hallmark of SARS-CoV-2 de-MARylation specificity. Virology 587:109845.<\/li>\n\n\n\n<li>Fadel F, et al (2022) Insights into the atypical autokinase activity of the <em>Pseudomonas aeruginosa<\/em> GacS histidine kinase and its interaction with RetS. Structure 30(9):1285-1297.e5.<\/li>\n\n\n\n<li>Fuchsbauer O, et al. (2019) Cas9 Allosteric Inhibition by the Anti-CRISPR Protein AcrIIA6. Mol Cell. 2765(19):30697-5.<\/li>\n\n\n\n<li>Bauer L, et al. (2019) Fluoxetine Inhibits Enterovirus Replication by Targeting the Viral 2C Protein in a Stereospecific Manner. ACS Infect Dis. 5 (9):1609-1623<\/li>\n\n\n\n<li>Tsika AC, et al. (2019) Deciphering the Nucleotide and RNA Binding Selectivity of the Mayaro Virus Macro Domain. J Mol Biol. 431(12):2283-2297<\/li>\n\n\n\n<li>Nguyen PC, et al. (2018) Biochemical and Structural Characterization of TesA, a Major Thioesterase Required for Outer-Envelope Lipid Biosynthesis in M.&nbsp;tuberculosis. J Mol Biol 430&nbsp;: 5120-5136<\/li>\n\n\n\n<li>Nguyen VS, et al. (2018) Towards a complete structural deciphering of Type VI secretion system. Curr Opin Struct Biol 49 77-84<\/li>\n\n\n\n<li>Leone P, et al. (2018) Type IX secretion system PorM and gliding machinery GldM form arches spanning the periplasmic space. Nat Commun 9 429<\/li>\n\n\n\n<li>Nguyen VS, et al. (2017) Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex. Nat Microbiol 2 17103<\/li>\n\n\n\n<li>Ravachol J, et al. (2016) Mechanisms involved in xyloglucan catabolism by the cellulosome-<\/li>\n\n\n\n<li>Foos N, et al. (2015) A flexible extension of the Drosophila ultrabithorax homeodomain defines a novel Hox\/PBC interaction mode. Structure 23(2):270-9<\/li>\n\n\n\n<li>De la Cruz MA, et al. (2013) A toxin-antitoxin module of Salmonella promotes virulence in mice. PLoS Pathog. 12:e1003827<\/li>\n\n\n\n<li>Mate MJ, et al. (2012) Crystal structure of the DNA-bound VapBC2 antitoxin\/toxin pair from Rickettsia felis. Nucleic Acids Res 40 3245-58<\/li>\n\n\n\n<li>Douzi B, et al. (2011) Deciphering the Xcp Pseudomonas aeruginosa type II secretion machinery through multiple interactions with substrates. J Biol Chem. 40 792-801<\/li>\n\n\n\n<li>Felisberto-Rodrigues C, et al. (2011) Towards a structural comprehension of bacterial type VI secretion systems&nbsp;: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathog. 7 e1002386<\/li>\n\n\n\n<li>Habchi J, et al. (2011) Characterization of the interactions between the nucleoprotein and the phosphoprotein of Henipavirus. J Biol Chem. 13 583-602<\/li>\n\n\n\n<li>Sciara G et al (2010) Structure of lactococcal phage p2 baseplate and its mechanism of activation. Proc Natl Acad Sci USA. 107 6852-7<\/li>\n\n\n\n<li>Veesler D, et al. (2009) Production and biophysical characterization of the CorA transporter from Methanosarcina mazei. Anal Biochem. 388 115-21.<\/li>\n<\/ul>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>This service offers a large panel of complementary equipment to  characterize molecular complexes and to screen or analyze molecular interactions, in solution or on solid support.<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":1060,"menu_order":4,"template":"","class_list":["post-1070","facility","type-facility","status-publish","hentry","entry"],"_links":{"self":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1070","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility"}],"about":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/types\/facility"}],"author":[{"embeddable":true,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/users\/1"}],"version-history":[{"count":12,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1070\/revisions"}],"predecessor-version":[{"id":11900,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1070\/revisions\/11900"}],"up":[{"embeddable":true,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1060"}],"wp:attachment":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/media?parent=1070"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}