{"id":1074,"date":"2021-03-29T18:06:14","date_gmt":"2021-03-29T17:06:14","guid":{"rendered":"http:\/\/www.afmb.univ-mrs.fr\/?post_type=facility&#038;p=1074"},"modified":"2025-01-29T18:39:46","modified_gmt":"2025-01-29T17:39:46","slug":"electron-microscopy","status":"publish","type":"facility","link":"https:\/\/www.afmb.univ-mrs.fr\/en\/facility\/structural-biology\/electron-microscopy\/","title":{"rendered":"Negative stain- and cryo-electron microscopy"},"content":{"rendered":"\n<div class=\"wp-block-group alignfull pageSubHeaderWrapper topSection has-white-color has-dark-blue-background-color has-text-color has-background\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<ul class=\"wp-block-list\">\n<li><a href=\"#presentation\">Pr\u00e9sentation<\/a><\/li>\n<\/ul>\n<\/div><\/div>\n\n\n\n<p><\/p>\n\n\n\n<p class=\"has-text-align-left has-normal-font-size\"><strong>TEM Glacios 200kV: Falcon 4i Camera + Selectris X Energy Filter<\/strong><\/p>\n\n\n\n<div class=\"wp-block-columns presentationTeamWrapper is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\" id=\"presentation\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<div class=\"wp-block-columns tableau is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div id=\"network\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"448\" height=\"597\" src=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2024\/02\/Glacios-web.jpg\" alt=\"\" class=\"wp-image-8838\" style=\"width:269px;height:auto\" srcset=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2024\/02\/Glacios-web.jpg 448w, https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2024\/02\/Glacios-web-375x500.jpg 375w\" sizes=\"auto, (max-width: 448px) 100vw, 448px\" \/><\/figure>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-8cf370e7 wp-block-group-is-layout-flex\">\n<p><strong>TEM Glacios at the AFMB lab<\/strong><\/p>\n\n\n\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"420\" height=\"433\" src=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2024\/02\/apo-full-2.jpg\" alt=\"\" class=\"wp-image-8827\" style=\"width:289px;height:auto\"\/><figcaption class=\"wp-element-caption\">Apoferritin structure at 1.9\u00c5 solved during the CWAT procedure at Brno in 2023<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-video\"><video controls src=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2024\/02\/apo-video-1.mp4\"><\/video><figcaption class=\"wp-element-caption\">Element of the apoferritin alpha helix<\/figcaption><\/figure>\n<\/div>\n<\/div>\n\n\n\n<figure class=\"wp-block-video\"><video controls src=\"https:\/\/www.afmb.univ-mrs.fr\/wp-content\/uploads\/2024\/04\/AFMB_Seminar_20240311_Denis-Ptchelkine.mp4\"><\/video><\/figure>\n\n\n\n<p class=\"has-text-align-left is-style-justified\">Cryo-EM field has been developing with unprecedented pace. Modern TEM allows collect data with increasing speed and high efficient robotic systems integrated in the microscope enable multiple experiments that can be done in fully automated non-assisted fashion. The EM workflow on modern microscopes is perfectly reliable and optimally set for quick evaluation of sample quality and setting up data acquisition.<\/p>\n\n\n\n<p class=\"has-text-align-left is-style-justified\">We welcome users from any life sciences labs. Assistance is offered during sample preparation and imaging sessions. Users can benefit from a prep lab at AFMB if any additional preparations need to be done to the samples. For grid freezing there are Vitrobot Mark4 and Leica GP available to users.<\/p>\n\n\n\n<p class=\"has-text-align-left\">Advanced EM users can also benefit from training offered by the platform. The training program can be tailored to specific user demands.<\/p>\n\n\n\n<p class=\"has-text-align-left\">While high-end microscope is available at our facility we strongly recommend users to test their samples by negative staining EM on a Tecnai 120 that is also available at our lab.<\/p>\n\n\n\n<p class=\"has-text-align-left\">No prior assessment of projects is necessary to access our facility but we do advise to get in touch with the facility manager to discuss the project before planning cryo-EM work.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div class=\"wp-block-group stickyColumnGroup\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\"><div class=\"singleTeamLeaders clearfix\"><div class=\"singleTeamContactWrapper\"><h5>Team contacts<\/h5><ul><li><a href=\"https:\/\/www.afmb.univ-mrs.fr\/en\/facility\/structural-biology\/electron-microscopy\/\" target=\"_blank\"><i class=\"fas fa-link\"><\/i><\/a><\/li><li><a href=\"mailto:denis.ptchelkine@univ-amu.fr\"><i class=\"far fa-envelope\"><\/i><\/a><\/li><li class=\"phoneIconWrapper\"><a class=\"phoneIconLink\"><i class=\"fas fa-tty\"><\/i><\/a><div class=\"phoneNumber\"><a href=\"tel:+33 (0)4 13 94 95 23\">+33 (0)4 13 94 95 23<\/a><\/div><\/li><\/ul><\/div><\/div>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-group accordeon\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading accordeonTitle\" id=\"publications\">Publications citing this service<\/h4>\n\n\n\n<div class=\"wp-block-group accordeonContent\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<ul class=\"wp-block-list\">\n<li>Nilsson J, Baroudi H, Gondelaud F, Pesce G, Bignon C, Ptchelkine D, Chamiech J, Cottet Herv\u00e9, Kajava A, Longhi S (2023) Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies. <strong>Int J Mol Sci<\/strong> 24:399<\/li>\n\n\n\n<li>Pesce G, Gondelaud F, Ptchelkine D, Nilsson J, Bignon C, Cartalas J, Fourquet P, Longhi S (2022) Experimental Evidence of Intrinsic Disorder and Amyloid Formation by the Henipavirus W Proteins <strong>Int J Mol Sci<\/strong> 23:923<\/li>\n\n\n\n<li>Gondelaud F, Pesce G, Nilsson J, Bignon C, Ptchelkine D, Gerlier D, Mathieu C, Longhi S (2022) Functional benefit of structural disorder for the replication of measles, Nipah and Hendra viruses. <strong>Essays in Bioc<\/strong> 66:915-934<\/li>\n\n\n\n<li>Bebeacua C, Tremblay D, Farenc C, Chapot-Chartier MP, Sadovskaya I, van Heel M, Veesler D, Moineau S, Cambillau C (2013) Structure, adsorption to host, and infection mechanism of virulent lactococcal phage p2. <strong>J Virol<\/strong> 87:12302-12.<\/li>\n\n\n\n<li>Desmyter A, Farenc C, Mahony J, Spinelli S, Bebeacua C, Blangy S, Veesler D, van Sinderen D, Cambillau C (2013) Viral infection modulation and neutralization by camelid nanobodies. <strong>Proc Natl Acad Sci U S A<\/strong> 110:E1371-9.<\/li>\n\n\n\n<li>Le Breton M, Meyniel-Schicklin L, Deloire A, Coutard B, Canard B, de Lamballerie X, Andre P, Rabourdin-Combe C, Lotteau V, Davoust N (2011) Flavivirus NS3 and NS5 proteins interaction network&nbsp;: a high-throughput yeast two-hybrid screen. <strong>BMC Microbiol<\/strong> 11, 234.<\/li>\n\n\n\n<li>Sciara G, Bebeacua C, Bron P, Tremblay D, Ortiz-Lombardia M, Lichi\u00e8re J, van Heel M, Campanacci V, Moineau S, Cambillau C (2010) Structure of lactococcal phage p2 baseplate and its mechanism of activation. <strong>Proc Natl Acad Sci USA<\/strong> 10:6852-7.<\/li>\n<\/ul>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>This service offers the expertise for the structural characterization of biological systems and large macromolecular assemblies by negative-staining (Tecnai 12) and cryo (Glacios 2) electron microscopy.<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":1060,"menu_order":6,"template":"","class_list":["post-1074","facility","type-facility","status-publish","hentry","entry"],"_links":{"self":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1074","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility"}],"about":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/types\/facility"}],"author":[{"embeddable":true,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/users\/1"}],"version-history":[{"count":56,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1074\/revisions"}],"predecessor-version":[{"id":10614,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1074\/revisions\/10614"}],"up":[{"embeddable":true,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/facility\/1060"}],"wp:attachment":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/media?parent=1074"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}