Location of the job<\/strong><\/p>\n\n\n\n Laboratory Architecture and Function of Biological Macromolecules (AFMB) in Marseille (https:\/\/www.afmb.univ-mrs.fr). The AFMB lab is a structural biology center, under the joint supervision of the CNRS and Aix-Marseille University and in partnership with INRAE \u200b\u200band INSERM. It is located on the Luminy campus in Marseille and is organized into 5 research teams sharing common services and technological platforms.<\/p>\n\n\n\n Scientific context: The work will take place within the AFMB lab, on the high-throughput recombinant protein production platform ((https:\/\/tinyurl.com\/ye254jad) under the direction of the manager, Dr. R. Vincentelli.<\/p>\n\n\n\n The people recruited will work on two distinct projects in which the aim is to produce, purify and characterize proteins at high throughput.<\/p>\n\n\n\n Project 1: ANR project; Full-PDZ-PBM HPVE6 on the high-throughput production and determination of the affinity of human PDZ domains for their partner pbms (PDZ binding motifs).<\/p>\n\n\n\n Project 2: CAZAI project funded by the Novo Nordisk foundation aiming to produce and purify enzymes (CAZymes) for their biochemical characterizations.<\/p>\n\n\n\n Missions and activities<\/strong><\/p>\n\n\n\n \u2022 Expression of the proteins in E. coli and purification by high-throughput methods on a Tecan robot and on an AKTA system.<\/p>\n\n\n\n \u2022 Ensure quality control of proteins by UPLC-MS.<\/p>\n\n\n\n \u2022 Implement, optimize, validate these protocols; identify potential problems.<\/p>\n\n\n\n \u2022 Use and present the results of the analyses, guarantee their monitoring and quality.<\/p>\n\n\n\n \u2022 Write experiment or study reports, technical notes, laboratory notebooks as well as progress reports.<\/p>\n\n\n\n \u2022 Manage and organize technical resources as part of a scientific project.<\/p>\n\n\n\n \u2022 Ensure the day-to-day management and maintenance of the machine park, stock of necessary materials and reagents, obtain quotes and order products in conjunction with the secretarial\/manager team.<\/p>\n\n\n\n \u2022 Ensure the application of hygiene and safety principles and rules.<\/p>\n\n\n\n \u2022 Participate in the dissemination and promotion of results in the form of oral presentations and publications.<\/p>\n\n\n\n Skills<\/strong><\/p>\n\n\n\n \u2022 Bac+5 training or equivalent.<\/p>\n\n\n\n \u2022 Interest in automated processes and high throughput.<\/p>\n\n\n\n o The recruited person will be trained on the Tecan automation systems and on the laboratory’s UPLC-MS (Waters) but knowledge of these systems would be a plus.<\/p>\n\n\n\n \u2022 Have theoretical and practical knowledge for the main techniques:<\/p>\n\n\n\n o Expression and production of proteins in Escherichia coli<\/p>\n\n\n\n o Protein biochemistry: purification by liquid chromatography (IMAC, affinity, ion exchange, gel filtration, etc.) on an automated device such as Tecan, Labchip GXII, AKTA, HPLC\/UPLC.<\/p>\n\n\n\n \u2022 Oral and written communication in English (level B1 to C1).<\/p>\n\n\n\n Personal qualities<\/strong><\/p>\n\n\n\n \u2022 Experimental rigor, sense of organization.<\/p>\n\n\n\n o Ability to adapt and autonomy.<\/p>\n\n\n\n o Relational skills, ability to work in a team.<\/p>\n\n\n\n o Interest in multidisciplinarity.<\/p>\n\n\n\n Position to be filled: from January\/February 2025.<\/p>\n\n\n\n Please send your application file (detailed CV + cover letter + people to contact for recommendations) by email in PDF format to Renaud Vincentelli (renaud.vincentelli@univ-amu.fr) with the subject: IE position application – project PDZ or CAZAI project (or both).<\/p>\n","protected":false},"excerpt":{"rendered":" 12-month fixed-term contract. Renewable. Full time. Remuneration according to Aix-Marseille-Universit\u00e9 salary scale. Location of the job Laboratory Architecture and Function […]<\/p>\n","protected":false},"author":3,"featured_media":702,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11],"class_list":["post-10073","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-positions-available","entry"],"_links":{"self":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/posts\/10073","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/comments?post=10073"}],"version-history":[{"count":8,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/posts\/10073\/revisions"}],"predecessor-version":[{"id":10334,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/posts\/10073\/revisions\/10334"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/media\/702"}],"wp:attachment":[{"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/media?parent=10073"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.afmb.univ-mrs.fr\/en\/wp-json\/wp\/v2\/categories?post=10073"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}