Juan Reguera
Senior researcher
Publications
2024
Alphavirus nsP3 organizes into tubular scaffolds essential for infection and the cytoplasmic granule architecture
Vasiliya Kril, Michael Hons, Celine Amadori, Claire Zimberger, Laurine Couture, Yara Bouery, Julien Burlaud-Gaillard, Andrei Karpov, Denis Ptchelkine, Alexandra Thienel, Beate Kümmerer, Ambroise Desfosses, Rhian Jones, Philippe Roingeard, Laurent Meertens, Ali Amara, Juan Reguera
Nature Communications 15:8106 (2024)10.1038/s41467-024-51952-z
Biophysical and structural study of La Crosse virus endonuclease inhibition for the development of new antiviral options
Mikael Feracci, Sergio Hernandez, Laura Garlatti, Clemence Mondielli, Renaud Vincentelli, Bruno Canard, Juan Reguera, François Ferron, Karine Alvarez
International Union of Crystallography journal 11:374-383 (2024)10.1107/S205225252400304X
2023
Structural basis of Ty1 integrase tethering to RNA polymerase III for targeted retrotransposon integration
Phong Quoc Nguyen, Sonia Huecas, Amna Asif-Laidin, Adrián Plaza-Pegueroles, Beatrice Capuzzi, Noé Palmic, Christine Conesa, Joël Acker, Juan Reguera, Pascale Lesage, Carlos Fernández-Tornero
Nature Communications 14:1729 (2023)10.1038/s41467-023-37109-4
Structures of active Hantaan virus polymerase uncover the mechanisms of Hantaviridae genome replication
Quentin Durieux Trouilleton, Sergio Barata-García, Benoît Arragain, Juan Reguera, Hélène Malet
Nature Communications 14:2954 (2023)10.1038/s41467-023-38555-w
Structural basis and dynamics of Chikungunya alphavirus RNA capping by nsP1 capping pores
Rhian Jones, Michael Hons, Nadia Rabah, Noelia Zamarreño, Rocío Arranz, Juan Reguera
Proceedings of the National Academy of Sciences of the United States of America 120 (2023)10.1073/pnas.2213934120
2021
Ty1 integrase is composed of an active N-terminal domain and a large disordered C-terminal module dispensable for its activity $in\ vitro$
Phong Quoc Nguyen, Christine Conesa, Elise Rabut, Gabriel Bragagnolo, Célia Gouzerh, Carlos Fernández-Tornero, Pascale Lesage, Juan Reguera, Joël Acker
Journal of Biological Chemistry 297:101093 (2021)10.1016/j.jbc.2021.101093
Structural Insights into the Mechanisms of Action of Functionally Distinct Classes of Chikungunya Virus Nonstructural Protein 1 Inhibitors
Kristina Kovacikova, Marina Gorostiola González, Rhian Jones, Juan Reguera, Alba Gigante, María-Jesús Pérez-Pérez, Gerhard Pürstinger, Julia Moesslacher, Thierry Langer, Lak Shin Jeong, Leen Delang, Johan Neyts, Eric Snijder, Gerard van Westen, Martijn van Hemert
Antimicrobial Agents and Chemotherapy 65 (2021)10.1128/AAC.02566-20
2020
Capping pores of alphavirus nsP1 gate membranous viral replication factories
Rhian Jones, Gabriel Bragagnolo, Rocío Arranz, Juan Reguera
Nature 589:615-619 (2020)10.1038/s41586-020-3036-8
Pre-initiation and elongation structures of full-length La Crosse virus polymerase reveal functionally important conformational changes
Benoît Arragain, Grégory Effantin, Piotr Gerlach, Juan Reguera, Guy Schoehn, Stephen Cusack, Hélène Malet
Nature Communications 11:3590 (2020)10.1038/s41467-020-17349-4
2019
High resolution cryo-EM structure of the helical RNA-bound Hantaan virus nucleocapsid reveals its assembly mechanisms
Benoît Arragain, Juan Reguera, Ambroise Desfosses, Irina Gutsche, Guy Schoehn, Hélène Malet
eLife 8 (2019)10.7554/eLife.43075
Structure and function of the Toscana virus cap-snatching endonuclease
Rhian Jones, Sana Lessoued, Kristina Meier, Stéphanie Devignot, Sergio Barata-García, Maria Mate, Gabriel Bragagnolo, Friedemann Weber, Maria Rosenthal, Juan Reguera
Nucleic Acids Research 47:10914-10930 (2019)10.1093/nar/gkz838
2017
Structural insights into reptarenavirus cap-snatching machinery
Maria Rosenthal, Nadja Gogrefe, Dominik Vogel, Juan Reguera, Bianka Rauschenberger, Stephen Cusack, Stephan Günther, Sophia Reindl
PLoS Pathogens 13:e1006400 (2017)10.1371/journal.ppat.1006400
Transcription and replication mechanisms of Bunyaviridae and Arenaviridae L proteins
Francois Ferron, Friedemann Weber, Juan de La Torre, Juan Reguera
Virus Research 234:118-134 (2017)10.1016/j.virusres.2017.01.018
2016
Comparative Structural and Functional Analysis of Bunyavirus and Arenavirus Cap-Snatching Endonucleases
Juan Reguera, Piotr Gerlach, Maria Rosenthal, Stephanie Gaudon, Francesca Coscia, Stephan Günther, Stephen Cusack
PLoS Pathogens 12:e1005636 (2016)10.1371/journal.ppat.1005636
Atomic Structure and Biochemical Characterization of an RNA Endonuclease in the N Terminus of Andes Virus L Protein
Yaiza Fernández-García, Juan Reguera, Carola Busch, Gregor Witte, Oliberto Sánchez-Ramos, Christian Betzel, Stephen Cusack, Stephan Günther, Sophia Reindl
PLoS Pathogens 12:e1005635 (2016)10.1371/journal.ppat.1005635
Towards a structural understanding of RNA synthesis by negative strand RNA viral polymerases
Juan Reguera, Piotr Gerlach, Stephen Cusack
Current Opinion in Structural Biology 36:75-84 (2016)10.1016/j.sbi.2016.01.002
2015
Structural Insights into Bunyavirus Replication and Its Regulation by the vRNA Promoter
Juan Reguera, Gaurav Mudgal, César Santiago, José Casasnovas
Cell 161:1267-1279 (2015)10.1016/j.cell.2015.05.006
2014
Segmented negative strand RNA virus nucleoprotein structure
Juan Reguera, Stephen Cusack, Daniel Kolakofsky
Current Opinion in Virology 5:7-15 (2014)10.1016/j.coviro.2014.01.003
2013
Structural basis for encapsidation of genomic RNA by La Crosse Orthobunyavirus nucleoprotein
Juan Reguera, Hélène Malet, Friedemann Weber, Stephen Cusack
Proceedings of the National Academy of Sciences of the United States of America 110:7246-7251 (2013)10.1073/pnas.1302298110
2012
Structural Bases of Coronavirus Attachment to Host Aminopeptidase N and Its Inhibition by Neutralizing Antibodies
Juan Reguera, César Santiago, Gaurav Mudgal, Desiderio Ordoño, Luis Enjuanes, José M Casasnovas
PLoS Pathogens 8:e1002859 (2012)10.1371/journal.ppat.1002859
2011
Antigenic modules in the N-terminal S1 region of the transmissible gastroenteritis virus spike protein
Juan Reguera, Desiderio Ordoño, César Santiago, Luis Enjuanes, José Casasnovas
Journal of General Virology 92:1117-1126 (2011)10.1099/vir.0.027607-0
2010
Bunyaviridae RNA Polymerases (L-Protein) Have an N-Terminal, Influenza-Like Endonuclease Domain, Essential for Viral Cap-Dependent Transcription
Juan Reguera, Friedemann Weber, Stephen Cusack
PLoS Pathogens 6:e1001101 (2010)10.1371/journal.ppat.1001101
2006
Nuclear Transport of Trimeric Assembly Intermediates Exerts a Morphogenetic Control on the Icosahedral Parvovirus Capsid
Laura Riolobos, Juan Reguera, Mauricio Mateu, José Almendral
Journal of Molecular Biology 357:1026-1038 (2006)10.1016/j.jmb.2006.01.019
2005
Functional Relevance of Amino Acid Residues Involved in Interactions with Ordered Nucleic Acid in a Spherical Virus
Juan Reguera, Esther Grueso, Aura Carreira, Cristina Sánchez-Martínez, José M Almendral, Mauricio G Mateu
Journal of Biological Chemistry 280:17969-17977 (2005)10.1074/jbc.m500867200
2004
Role of interfacial amino acid residues in assembly, stability, and conformation of a spherical virus capsid
Juan Reguera, Aura Carreira, Laura Riolobos, José María Almendral, Mauricio Mateu
Proceedings of the National Academy of Sciences of the United States of America 101:2724-2729 (2004)10.1073/pnas.0307748101
2003
In Vitro Disassembly of a Parvovirus Capsid and Effect on Capsid Stability of Heterologous Peptide Insertions in Surface Loops
Aura Carreira, Margarita Menéndez, Juan Reguera, José María Almendral, Mauricio G Mateu
Journal of Biological Chemistry 279:6517-6525 (2003)10.1074/jbc.m307662200
