Current position:
Biological Techniques Engineer CNRS (AFMB-UMR7257) since 2016
Prevention Assistant at AFMB-Polytech site since 2018
Laser Safety Manager since 2020

Research topics:
Enzymatic and structural characterizations of emerging virus replicases (Dengue, Zika, SARS-CoV-2 etc…).

Activities :
Molecular cloning, site-directed mutagenesis, expression of recombinant proteins in bacterial systems, protein purification, radioactive and fluorescent enzyme assays, inhibition assays, crystallogenesis, student supervision and training of new entrants.

Diploma :
Master 2 Microbiology, Plant Biology and Biotechnology (MBVB)

Publications

2022

2022
A dual mechanism of action of AT-527 against SARS-CoV-2 polymerase

Ashleigh Shannon, Véronique Fattorini, Bhawna Sama, Barbara Selisko, Mikael Feracci, Camille Falcou, Pierre Gauffre, Priscila El Kazzi, Adrien Delpal, Etienne Decroly, Karine Alvarez, Cécilia Eydoux, Jean-Claude Guillemot, Adel Moussa, Steven S Good, Paolo La Colla, Kai Lin, Jean-Pierre Sommadossi, yingxiao Zhu, Xiaodong yan, Hui Shi, Francois Ferron, Bruno Canard

Nature Communications 13 (2022)10.1038/s41467-022-28113-1

2021

2021
A fluorescence-based high throughput-screening assay for the SARS-CoV RNA synthesis complex

Cecilia Eydoux, Veronique Fattorini, Ashleigh Shannon, Thi-Tuyet-Nhung Le, Bruno Didier, Bruno Canard, Jean-Claude Guillemot

Journal of Virological Methods 288:114013 (2021)10.1016/j.jviromet.2020.114013

2020

2020
Rapid incorporation of Favipiravir by the fast and permissive viral RNA polymerase complex results in SARS-CoV-2 lethal mutagenesis

Ashleigh Shannon, Barbara Selisko, Nhung-Thi-Tuyet Le, Johanna Huchting, Franck Touret, Géraldine Piorkowski, Véronique Fattorini, Francois Ferron, Etienne Decroly, Chris Meier, Bruno Coutard, Olve Peersen, Bruno Canard

Nature Communications 11:4682 (2020)10.1038/s41467-020-18463-z

2017

2017
Substrate selectivity of Dengue and Zika virus NS5 polymerase towards 2′-modified nucleotide analogues

Supanee Potisopon, Francois Ferron, Véronique Fattorini, Barbara Selisko, Bruno Canard

Antiviral Research 140:25-36 (2017)10.1016/j.antiviral.2016.12.021