J. Reguera’s team guest: Michael Hons, EMBL Grenoble – “Exploring structures in Chikungunya viral replication and in neural axon fate by cryo-EM”

Abstract

Within EMBL Grenoble, I explored by cryo-EM structures related to Chikungunya Viral Replication (1) and Axonal Wallerian Degeneration (2) and we developed a cryo-EM automatization pipeline called ‘Easygrid’ (3). I will present how we use single-particle cryo-EM to structurally decipher the role of nsP1/3 in Chikungunya Viral Replication (1) and the process of Wallerian Degeneration in Axons (2). I will introduce Easygrid (3), an automated cryo-EM grid preparation and evaluation pipeline for single-particle and in situ cryo-EM. From human pathogenic Chikungunya Virus, I will build a bridge towards another +ssRNA virus: Plant pathogenic Potyvirus Y. I will present how I aim to study Potyvirus Y Replication using single-particle and in situ cryo-EM approaches.