Etienne Decroly
Senior researcher
Research group: Viral Replicases: Structure, function and drug-design
- 1994 – PhD thesis Université Libre de Bruxelles (ULB):
“Contribution to the study of the envelope glycoprotein (gp160) of HIV-1 virus”.
Laboratory of Physical Chemistry of Macromolecules at Interfaces, Professor J.-M. Ruysschaert (Brussels, Belgium). - 1995 to 1997: Postdoctoral training at the Clinical Research Institute of Montreal (IRCM):
“Study of the involvement of protein convertases in the maturation process of the envelope glycoprotein (gp160) of the HIV virus”. Neuroendocrine Biochemistry Laboratory, Dr. N.G. Seidah (Montreal, Canada). - 1998 to 2001: 2nd post-doctoral internship, Co-leader of the VIF team at INSERM-U372
“Study of the involvement of the HIV Vif protein in the control of cellular antiviral defenses
Laboratory: Pathogenesis of Lentivirus Infections, Dr. R. Vigne (Marseille, France). - Since 2005 CNRS Research Fellow (CR1) at AFMB-UMR7257
“Identification and characterization of viral enzymes involved in RNA virus cap formation”.
AFMB laboratory, Viral replication, structure, mechanisms & Drug-design, Dr. B. Canard (Marseille, France). - 2011 HDR Aix Marseille University
- 2012 DR2 CNRS
Teaching activity at * Aix Marseille University * Institut Pasteur/Université Paris 6 * Université du Sud/Toulon Var
Publications
2024
JNJ-7184, a respiratory syncytial virus inhibitor targeting the connector domain of the viral polymerase
Brecht Bonneux, Afzaal Shareef, Sergey Tcherniuk, Brandon Anson, Suzanne de Bruyn, Nick Verheyen, Kim Thys, Nádia Conceição-Neto, Marcia van Ginderen, Leen Kwanten, Nina Ysebaert, Luc Vranckx, Elien Peeters, Ellen Lanckacker, Jack M Gallup, Panchan Sitthicharoenchai, Sarhad Alnajjar, Mark R Ackermann, Suraj Adhikary, Anusarka Bhaumik, Aaron Patrick, Amy Fung, Priscila Sutto-Ortiz, Etienne Decroly, Stephen W Mason, David Lançois, Jerome Deval, Zhinan Jin, Jean-François Éléouët, Rachel Fearns, Anil Koul, Dirk Roymans, Peter Rigaux, Florence Herschke
Antiviral Research 227:105907 (2024)10.1016/j.antiviral.2024.105907
Effets de la 2′-O-méthylation de l’ARN génomique du VIH-1 sur la réplication virale
Alice Decombe, Priscila El-Kazzi, Sébastien Nisole, Étienne Decroly
Médecine/Sciences 40:421-427 (2024)10.1051/medsci/2024046
5′-cap RNA/SAM mimetic conjugates as bisubstrate inhibitors of viral RNA cap 2′-O-methyltransferases
Rostom Ahmed‐belkacem, Priscila Sutto-Ortiz, Adrien Delpal, Joris Troussier, Bruno Canard, Jean-Jacques Vasseur, Etienne Decroly, Françoise Debart
Bioorganic Chemistry 143:107035 (2024)10.1016/j.bioorg.2023.107035
N-arylsulfonamide-based adenosine analogues to target RNA cap N7-methyltransferase nsp14 of SARS-CoV-2
Rostom Ahmed‐belkacem, Joris Troussier, Adrien Delpal, Bruno Canard, Jean-Jacques Vasseur, Etienne Decroly, Françoise Debart
RSC Medicinal Chemistry (2024)10.1039/d3md00737e
2023
Structural and mechanistic insights into the inhibition of respiratory syncytial virus polymerase by a non-nucleoside inhibitor
Xiaodi Yu, Pravien Abeywickrema, Brecht Bonneux, Ishani Behera, Brandon Anson, Edgar Jacoby, Amy Fung, Suraj Adhikary, Anusarka Bhaumik, Rodrigo Carbajo, Suzanne de Bruyn, Robyn Miller, Aaron Patrick, Quyen Pham, Madison Piassek, Nick Verheyen, Afzaal Shareef, Priscila Sutto-Ortiz, Nina Ysebaert, Herman van Vlijmen, Tim Jonckers, Florence Herschke, Jason Mclellan, Etienne Decroly, Rachel Fearns, Sandrine Grosse, Dirk Roymans, Sujata Sharma, Peter Rigaux, Zhinan Jin
Communications Biology 6:1074 (2023)10.1038/s42003-023-05451-4
Internal RNA 2′-O-methylations on HIV-1 genome impair reverse transcription.
Alice Decombe, Olve Peersen, Priscila Sutto-Ortiz, Célia Chamontin, Géraldine Piorkowski, Bruno Canard, Sébastien Nisole, Etienne Decroly
Nucleic Acids Research (2023)
Internal RNA 2′- O -methylation on the HIV-1 genome impairs reverse transcription
Alice Decombe, Olve Peersen, Priscila Sutto-Ortiz, Célia Chamontin, Géraldine Piorkowski, Bruno Canard, Sébastien Nisole, Etienne Decroly
Nucleic Acids Research (2023)10.1093/nar/gkad1134
Tick‐borne flavivirus NS5 antagonizes interferon signaling by inhibiting the catalytic activity of TYK2
Ségolène Gracias, Maxime Chazal, Alice Decombe, Yves Unterfinger, Adrià Sogues, Lauryne Pruvost, Valentine Robert, Sandrine Lacour, Manon Lemasson, Marion Sourisseau, Zhi Li, Jennifer Richardson, Sandra Pellegrini, Etienne Decroly, Vincent Caval, Nolwenn Jouvenet
EMBO Reports 24:e57424 (2023)10.15252/embr.202357424
Protéine 2C d’Entérovirus : protéine clé de la réplication virale et cible antivirale
Priscila El Kazzi, Carole Yaacoub, Ziad Fajloun, Patrice Vanelle, Etienne Decroly, Bruno Coutard, Karine Barral
Virologie 27:173-188 (2023)10.1684/vir.2023.1001
Interplay of RNA 2′-O-methylations with viral replication
Alice Decombe, Priscila El Kazzi, Etienne Decroly
Current Opinion in Virology 59:101302 (2023)10.1016/j.coviro.2023.101302
Design and synthesis of naturally-inspired SARS-CoV-2 inhibitors
Haitham Hassan, Jeanne Chiavaralli, Afnan Hassan, Loay Bedda, Tim Krischuns, Kuang-Yu Chen, Alice Shi Ming Li, Adrien Delpal, Etienne Decroly, Masoud Vedadi, Nadia Naffakh, Fabrice Agou, Sergio Mallart, Reem Arafa, Paola Arimondo
RSC Medicinal Chemistry 14:507-519 (2023)10.1039/D2MD00149G
Biochemistry of the Respiratory Syncytial Virus L Protein Embedding RNA Polymerase and Capping Activities
Priscila Sutto-Ortiz, Jean-François Eléouët, François Ferron, Etienne Decroly
Viruses 15:341 (2023)10.3390/v15020341
AT-752 targets multiple sites and activities on the Dengue virus replication enzyme NS5
Mikael Feracci, Cécilia Eydoux, Véronique Fattorini, Lea Lo Bello, Pierre Gauffre, Barbara Selisko, Priscila Sutto-Ortiz, Ashleigh Shannon, Hongjie Xia, Pei-Yong Shi, Mathieu Noel, Françoise Debart, Jean-Jacques Vasseur, Steve Good, Kai Lin, Adel Moussa, Jean-Pierre Sommadossi, Aurélie Chazot, Karine Alvarez, Jean-Claude Guillemot, Etienne Decroly, François Ferron, Bruno Canard
Antiviral Research 212:105574 (2023)10.1016/j.antiviral.2023.105574
Amino Acid Polymorphisms on the Brazilian Strain of Yellow Fever Virus Methyltransferase Are Related to the Host’s Immune Evasion Mediated by Type I Interferon
Nathália Dias Furtado, Iasmim Silva de Mello, Andre Schutzer de Godoy, Gabriela Dias Noske, Glaucius Oliva, Bruno Canard, Etienne Decroly, Myrna Bonaldo
Viruses 15:191 (2023)10.3390/v15010191
Structure-guided optimization of adenosine mimetics as selective and potent inhibitors of coronavirus nsp14 N7-methyltransferases
Marcel Hausdorff, Adrien Delpal, Sarah Barelier, Laura Nicollet, Bruno Canard, Franck Touret, Agathe Colmant, Bruno Coutard, Jean-Jacques Vasseur, Etienne Decroly, Françoise Debart
European Journal of Medicinal Chemistry (2023)10.1016/j.ejmech.2023.115474
2C protein of Enterovirus: key protein of viral replication and antiviral target
Priscila El Kazzi, Carole Yaacoub, Ziad Fajloun, Patrice Vanelle, Etienne Decroly, Bruno Coutard, Karine Barral
Virologie 27:35-49 (2023)10.1684/vir.2023.1002
2022
Internal RNA 2′O-methylation in the HIV-1 genome counteracts ISG20 nuclease-mediated antiviral effect
Priscila El Kazzi, Nadia Rabah, Célia Chamontin, Lina Poulain, François Ferron, Françoise Debart, Bruno Canard, Dorothée Missé, Bruno Coutard, Sébastien Nisole, Etienne Decroly
Nucleic Acids Research (2022)10.1093/nar/gkac996
A second type of N7-guanine RNA cap methyltransferase in an unusual locus of a large RNA virus genome
Ashleigh Shannon, Bhawna Sama, Pierre Gauffre, Théo Guez, Françoise Debart, Jean-Jacques Vasseur, Etienne Decroly, Bruno Canard, François Ferron
Nucleic Acids Research 50:11186-11198 (2022)10.1093/nar/gkac876
Identification of potent inhibitors of arenavirus and SARS-CoV-2 exoribonucleases by fluorescence polarization assay
Sergio Hernández, Mikael Feracci, Carolina Trajano de Jesus, Priscila El Kazzi, Rafik Kaci, Laura Garlatti, Clemence Mondielli, Fabrice Bailly, Philippe Cotelle, Franck Touret, Xavier de Lamballerie, Bruno Coutard, Etienne Decroly, Bruno Canard, François Ferron, Karine Alvarez
Antiviral Research 204:105364 (2022)10.1016/j.antiviral.2022.105364
A Versatile Class of 1,4,4-Trisubstituted Piperidines Block Coronavirus Replication In Vitro
Sonia de Castro, Annelies Stevaert, Miguel Maldonado, Adrien Delpal, Julie Vandeput, Benjamin van Loy, Cecilia Eydoux, Jean-Claude Guillemot, Etienne Decroly, Federico Gago, Bruno Canard, Maria-Jose Camarasa, Sonsoles Velázquez, Lieve Naesens
Pharmaceuticals 15:1021 (2022)10.3390/ph15081021
A highly sensitive cell-based luciferase assay for high-throughput automated screening of SARS-CoV-2 nsp5/3CLpro inhibitors
K.Y. Chen, T. Krischuns, L. Ortega Varga, E. Harigua-Souiai, S. Paisant, A. Zettor, J. Chiaravalli, A. Delpal, D. Courtney, A. O'Brien, S.C. Baker, Etienne Decroly, C. Isel, F. Agou, Y. Jacob, A. Blondel, Nadia Naffakh
Antiviral Research 201:105272 (2022)10.1016/j.antiviral.2022.105272
Potent Inhibition of SARS-CoV-2 nsp14 N 7-Methyltransferase by Sulfonamide-Based Bisubstrate Analogues
Rostom Ahmed‐belkacem, Marcel Hausdorff, Adrien Delpal, Priscila Sutto-Ortiz, Agathe M G Colmant, Franck Touret, Natacha S Ogando, Eric J Snijder, Bruno Canard, Bruno Coutard, Jean-Jacques Vasseur, Etienne Decroly, Françoise Debart
Journal of Medicinal Chemistry 65:6231-6249 (2022)10.1021/acs.jmedchem.2c00120
Distinctive Roles of Furin and TMPRSS2 in SARS-CoV-2 Infectivity
Rachid Essalmani, Jaspreet Jain, Delia Susan-Resiga, Ursula Andréo, Alexandra Evagelidis, Rabeb Mouna Derbali, David Huynh, Frédéric Dallaire, Mélanie Laporte, Adrien Delpal, Priscila Sutto-Ortiz, Bruno Coutard, Claudine Mapa, Keith Wilcoxen, Etienne Decroly, Tram Nq Pham, Éric Cohen, Nabil Seidah
Journal of Virology 96 (2022)10.1128/jvi.00128-22
A dual mechanism of action of AT-527 against SARS-CoV-2 polymerase
Ashleigh Shannon, Véronique Fattorini, Bhawna Sama, Barbara Selisko, Mikael Feracci, Camille Falcou, Pierre Gauffre, Priscila El Kazzi, Adrien Delpal, Etienne Decroly, Karine Alvarez, Cécilia Eydoux, Jean-Claude Guillemot, Adel Moussa, Steven S Good, Paolo La Colla, Kai Lin, Jean-Pierre Sommadossi, Yingxiao Zhu, Xiaodong Yan, Hui Shi, Francois Ferron, Bruno Canard
Nature Communications 13 (2022)10.1038/s41467-022-28113-1
Fluoxetine targets an allosteric site in the enterovirus 2C AAA+ ATPase and stabilizes a ring-shaped hexameric complex
Daniel L Hurdiss, Priscila El Kazzi, Lisa Bauer, Nicolas Papageorgiou, Francois Ferron, Tim Donselaar, Arno L.W. van Vliet, Tatiana M Shamorkina, Joost Snijder, Bruno Canard, Etienne Decroly, Andrea Brancale, Tzviya Zeev-Ben-Mordehai, Friedrich Förster, Frank J.M. van Kuppeveld, Bruno Coutard
Science Advances 8 (2022)10.1126/sciadv.abj7615
Facile access to 4′-( N -acylsulfonamide) modified nucleosides and evaluation of their inhibitory activity against SARS-CoV-2 RNA cap N 7-guanine-methyltransferase nsp14
Romain Amador, Adrien Delpal, Bruno Canard, Jean-Jacques Vasseur, Etienne Decroly, Françoise Debart, Guillaume Clavé, Michael Smietana
Organic & Biomolecular Chemistry 20:7582-7586 (2022)10.1039/d2ob01569b
Synthesis, Structure–Activity Relationships, and Antiviral Profiling of 1-Heteroaryl-2-Alkoxyphenyl Analogs As Inhibitors of SARS-CoV-2 Replication
Dorothée Bardiot, Laura Vangeel, Mohamed Koukni, Philippe Arzel, Marleen Zwaagstra, Heyrhyoung Lyoo, Patrick Wanningen, Shamshad Ahmad, Linlin Zhang, Xinyuanyuan Sun, Adrien Delpal, Cecilia Eydoux, Jean-Claude Guillemot, Eveline Lescrinier, Hugo Klaassen, Pieter Leyssen, Dirk Jochmans, Karolien Castermans, Rolf Hilgenfeld, Colin Robinson, Etienne Decroly, Bruno Canard, Eric J Snijder, Martijn J Van Hemert, Frank Van van Kuppeveld, Patrick Chaltin, Johan Neyts, Steven De de Jonghe, Arnaud Marchand
Molecules 27:1052 (2022)10.3390/molecules27031052
2021
Structure-function analysis of the nsp14 N7-guanine methyltransferase reveals an essential role in Betacoronavirus replication
Natacha S Ogando, Priscila El Kazzi, Jessika C Zevenhoven-Dobbe, Brenda W Bontes, Alice Decombe, Clara C S Posthuma, Volker Thiel, Bruno Canard, Francois Ferron, Etienne Decroly, Eric J Snijder
Proceedings of the National Academy of Sciences of the United States of America (2021)10.1101/2021.05.17.444407
System-oriented optimization of multi-target 2,6-diaminopurine derivatives: Easily accessible broad-spectrum antivirals active against flaviviruses, influenza virus and SARS-CoV-2
Ilaria Vicenti, Maria Grazia Martina, Adele Boccuto, Marta de Angelis, Giorgia Giavarini, Filippo Dragoni, Serena Marchi, Claudia Maria Trombetta, Emmanuele Crespan, Giovanni Maga, Cecilia Eydoux, Etienne Decroly, Emanuele Montomoli, Lucia Nencioni, Maurizio Zazzi, Marco Radi
European Journal of Medicinal Chemistry 224:113683 (2021)10.1016/j.ejmech.2021.113683
An appeal for an objective, open, and transparent scientific debate about the origin of SARS-CoV-2
Jacques van Helden, Colin Butler, Guillaume Achaz, Bruno Canard, Didier Casane, Jean-Michel Claverie, Fabien Colombo, Virginie Courtier-Orgogozo, Richard Ebright, François Graner, Milton Leitenberg, Serge Morand, Nikolai Petrovsky, Rossana Segreto, Etienne Decroly, José Halloy
The Lancet 398:1402-1404 (2021)10.1016/S0140-6736(21)02019-5
Structure and Sequence Requirements for RNA Capping at the Venezuelan Equine Encephalitis Virus RNA 5′ End
Oney Ortega Granda, Coralie Valle, Ashleigh Shannon, Etienne Decroly, Bruno Canard, Bruno Coutard, Nadia Rabah
Journal of Virology 95 (2021)10.1128/JVI.00777-21
The enzymes for genome size increase and maintenance of large (+)RNA viruses
Francois Ferron, Bhawna Sama, Etienne Decroly, Bruno Canard
Trends in Biochemical Sciences (2021)10.1016/j.tibs.2021.05.006
Tracing the origins of SARS-COV-2 in coronavirus phylogenies: a review
Erwan Sallard, José Halloy, Didier Casane, Etienne Decroly, Jacques van Helden
Environmental Chemistry Letters 19:769-785 (2021)10.1007/s10311-020-01151-1
Dengue Virus NS5 Transcribes Metabolite-Capped, RIG-I Sensitive vRNAs
Brandon Schweibenz, Mihai Solotchi, Etienne Decroly, Barbara Selisko, Bruno Canard, Smita Patel
Biophysical Journal 120:135a (2021)10.1016/j.bpj.2020.11.1017
COVID-19 epidemiologic surveillance using wastewater
Virender Sharma, Chetan Jinadatha, Eric Lichtfouse, Etienne Decroly, Jacques van Helden, Hosoon Choi, Piyali Chatterjee
Environmental Chemistry Letters 19:1911-1915 (2021)10.1007/s10311-021-01188-w
The methyltransferase domain of the Respiratory Syncytial Virus L protein catalyzes cap N7 and 2’-O-methylation
Priscila Sutto-Ortiz, Sergey Tcherniuk, Nina Ysebaert, Pravien Abeywickrema, Mathieu Noël, Alice Decombe, Françoise Debart, Jean-Jacques Vasseur, Bruno Canard, Dirk Roymans, Peter Rigaux, Jean-François Eléouët, Etienne Decroly
PLoS Pathogens 17:1-20 (2021)10.1371/journal.ppat.1009562
The WHO mission report struggles to trace the origins of the SARS-CoV-2 epidemic
Etienne Decroly, Jean-Michel Claverie, Bruno Canard
Virologie 1-7 (2021)
First insights into the structural features of Ebola virus methyltransferase activities
Coralie Valle, Baptiste Martin, François Ferron, Véronique Roig-Zamboni, Aline Desmyter, Françoise Debart, Jean Jacques Vasseur, Bruno Canard, Bruno Coutard, Etienne Decroly
Nucleic Acids Research 49:1737-1748 (2021)10.1093/nar/gkaa1276
2020
Tracing the origins of SARS-CoV-2 in coronavirus phylogenies
Erwan Sallard, José Halloy, Didier Casane, Etienne Decroly, Jacques van Helden
Médecine/Sciences 36 (2020)10.1051/medsci/2020123
Drugs against SARS‐CoV ‐2: What do we know about their mode of action?
Coralie Valle, Baptiste Martin, Franck Touret, Ashleigh Shannon, Bruno Canard, Jean‐claude Guillemot, Bruno Coutard, Etienne Decroly
Reviews in Medical Virology 30:1-10 (2020)10.1002/rmv.2143
Synthesis and biological evaluation of novel flexible nucleoside analogues that inhibit flavivirus replication in vitro
Joy Thames, Charles Waters, Coralie Valle, Marcella Bassetto, Wahiba Aouadi, Baptiste Martin, Barbara Selisko, Arissa Falat, Bruno Coutard, Andrea Brancale, Bruno Canard, Etienne Decroly, Katherine Seley-Radtke
Bioorganic and Medicinal Chemistry Letters 28:115713 (2020)10.1016/j.bmc.2020.115713
Structure-based drug repositioning over the human TMPRSS2 protease domain: search for chemical probes able to repress SARS-CoV-2 Spike protein cleavages
Natesh Singh, Etienne Decroly, Abdel-Majid Khatib, Bruno Villoutreix
European Journal of Pharmaceutical Sciences 153:105495 (2020)10.1016/j.ejps.2020.105495
Mutations on VEEV nsP1 relate RNA capping efficiency to ribavirin susceptibility
Nadia Rabah, Oney Ortega Granda, Gilles Quérat, Bruno Canard, Etienne Decroly, Bruno Coutard
Antiviral Research 182:104883 (2020)10.1016/j.antiviral.2020.104883
Rapid incorporation of Favipiravir by the fast and permissive viral RNA polymerase complex results in SARS-CoV-2 lethal mutagenesis
Ashleigh Shannon, Barbara Selisko, Nhung-Thi-Tuyet Le, Johanna Huchting, Franck Touret, Géraldine Piorkowski, Véronique Fattorini, Francois Ferron, Etienne Decroly, Chris Meier, Bruno Coutard, Olve Peersen, Bruno Canard
Nature Communications 11:4682 (2020)10.1038/s41467-020-18463-z
Synthesis of adenine dinucleosides SAM analogs as specific inhibitors of SARS-CoV nsp14 RNA cap guanine-N7-methyltransferase
Rostom Ahmed‐belkacem, Priscila Sutto-Ortiz, Mathis Guiraud, Bruno Canard, Jean-Jacques Vasseur, Etienne Decroly, Francoise Debart
European Journal of Medicinal Chemistry 201:112557 (2020)10.1016/j.ejmech.2020.112557
In vitro screening of a FDA approved chemical library reveals potential inhibitors of SARS-CoV-2 replication
Franck Touret, Magali Gilles, Karine Barral, Antoine Nougairède, Jacques van Helden, Etienne Decroly, Xavier de Lamballerie, Bruno Coutard
Scientific Reports 10:13093 (2020)10.1038/s41598-020-70143-6
Novel Class of Chikungunya Virus Small Molecule Inhibitors That Targets the Viral Capping Machinery
Rana Abdelnabi, Kristina Kovacikova, Julia Moesslacher, Kim Donckers, Verena Battisti, Pieter Leyssen, Thierry Langer, Gerhard Puerstinger, Gilles Quérat, Changqing Li, Etienne Decroly, Ali Tas, Arnaud Marchand, Patrick Chaltin, Bruno Coutard, Martijn van Hemert, Johan Neyts, Leen Delang
Antimicrobial Agents and Chemotherapy 64 (2020)10.1128/AAC.00649-20
To something, Covid is good: SFV 2.0
Grégory Caignard, Etienne Decroly, Maria Dimitrova, David Gilmer, Anne-Sophie Gosselin-Grenet, Henri Gruffat, Sandra Martin-Latil, Pascale Massin, Sandie Munier, Mylène Ogliastro, Aure Saulnier, Noël Tordo
Virologie 24:133-134 (2020)10.1684/vir.2020.0846
Remdesivir and SARS-CoV-2: Structural requirements at both nsp12 RdRp and nsp14 Exonuclease active-sites
Ashleigh Shannon, Nhung Thi-Tuyet Le, Barbara Selisko, Cecilia Eydoux, Karine Alvarez, Jean-Claude Guillemot, Etienne Decroly, Olve Peersen, Francois Ferron, Bruno Canard
Antiviral Research 178:104793 (2020)10.1016/j.antiviral.2020.104793
To something, Covid is good: SFV 2.0
Grégory Caignard, Etienne Decroly, Maria Dimitrova, David Gilmer, Anne-Sophie Gosselin-Grenet, Henri Gruffat, Sandra Martin-Latil, Pascale Massin, Sandie Munier, Mylène Ogliastro, Aure Saulnier, Noël Tordo
Virologie 24:133-134 (2020)10.1684/vir.2020.0846
A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae
Francois Ferron, Humberto Julio Debat, Ashleigh Shannon, Etienne Decroly, Bruno Canard
NAR Genomics and Bioinformatics 2 (2020)10.1093/nargab/lqz022
Design, Synthesis and Discovery of N,N’ ‐Carbazoyl‐aryl‐urea Inhibitors of Zika NS5 Methyltransferase and Virus Replication
Sharon Spizzichino, Giulio Mattedi, Kate Lauder, Coralie Valle, Wahiba Aouadi, Bruno Canard, Etienne Decroly, Suzanne Kaptein, Johan Neyts, Carl Graham, Zakary Sule, David Barlow, Romano Silvestri, Daniele Castagnolo
ChemMedChem 15:385-390 (2020)10.1002/cmdc.201900533
The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade
Bruno Coutard, Coralie Valle, Xavier de Lamballerie, Bruno Canard, Nabil G Seidah, Etienne Decroly
Antiviral Research 176:104742 (2020)10.1016/j.antiviral.2020.104742
The C-Terminal Domain of the Sudan Ebolavirus L Protein Is Essential for RNA Binding and Methylation
Coralie Valle, Baptiste Martin, Francoise Debart, Jean-Jacques Vasseur, Isabelle Imbert, Bruno Canard, Bruno Coutard, Etienne Decroly
Journal of Virology 94:e00520-20 (2020)10.1128/JVI.00520-20
2019
Structure of the Respiratory Syncytial Virus Polymerase Complex
Morgan S.A. Gilman, Cheng Liu, Amy Fung, Ishani Behera, Paul Jordan, Peter Rigaux, Nina Ysebaert, Sergey Tcherniuk, Julien Sourimant, Jean-François Éléouët, Priscila Sutto-Ortiz, Etienne Decroly, Dirk Roymans, Zhinan Jin, Jason S Mclellan
Cell 179:193-204.e14 (2019)10.1016/j.cell.2019.08.014
Structure of the Respiratory Syncytial Virus Polymerase Complex
Morgan S A Gilman, Cheng Liu, Amy Fung, Ishani Behera, Paul Jordan, Peter Rigaux, Nina Ysebaert, Sergey Tcherniuk, Julien Sourimant, Jean-Francois Eleouet, Priscila Sutto-Ortiz, Etienne Decroly, Dirk Roymans, Zhinan Jin, Jason S Mclellan
Cell 179:193-204.e14 (2019)10.1016/j.cell.2019.08.014
The Curious Case of the Nidovirus Exoribonuclease: Its Role in RNA Synthesis and Replication Fidelity
Natacha S Ogando, Francois Ferron, Etienne Decroly, Bruno Canard, Clara C Posthuma, Eric J Snijder
Frontiers in Microbiology 10 (2019)10.3389/fmicb.2019.01813
C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
Philippe H Jaïs, Etienne Decroly, Eric Jacquet, Marine Le boulch, Aurélien Jaïs, Olivier Jean-Jean, Heather Eaton, Prishila Ponien, Frédérique Verdier, Bruno Canard, Sergio Gonçalves, Stéphane Chiron, Maude Le Gall, Patrick Mayeux, Maya Shmulevitz
Nucleic Acids Research 47:2681-2698 (2019)10.1093/nar/gkz069
Approved drugs screening against the nsP1 capping enzyme of Venezuelan equine encephalitis virus using an immuno-based assay
Ana Ferreira-Ramos, Changqing Li, Cecilia Eydoux, Jean Marie Contreras, Christophe Morice, Gilles Querat, Alba Gigante, María-Jesús Pérez Pérez, Marie-Louise Jung, Bruno Canard, Jean-Claude Guillemot, Etienne Decroly, Bruno Coutard
Antiviral Research 163:59-69 (2019)10.1016/j.antiviral.2019.01.003
FTSJ3 is an RNA 2′-O-methyltransferase recruited by HIV to avoid innate immune sensing
Mathieu Ringeard, Virginie Marchand, Etienne Decroly, Yuri Motorin, Yamina Bennasser
Nature 565:500-504 (2019)10.1038/s41586-018-0841-4
Optimization of a fragment linking hit toward Dengue and Zika virus NS5 methyltransferases inhibitors
Jessica Hernandez, Laurent Hoffer, Bruno Coutard, Gilles Quérat, Philippe Roche, Xavier Morelli, Etienne Decroly, Karine Barral
European Journal of Medicinal Chemistry 161:323-333 (2019)10.1016/j.ejmech.2018.09.056
Synthesis of Adenine Dinucleosides 2′,5′-Bridged by Sulfur-Containing Linkers as Bisubstrate SAM Analogues for Viral RNA 2′- O -Methyltransferases
Rostom Ahmed‐belkacem, Priscila Sutto Ortiz, Etienne Decroly, Jean-Jacques Vasseur, Françoise Debart
European Journal of Organic Chemistry 2019 (2019)10.1002/ejoc.201901120
2018
Ebola virus L protein harbors a new enzymatic activity involved in the internal methylation of RNAs
Baptiste Martin, Coralie Valle, Bruno Coutard, Bruno Canard, Françoise Debart, Etienne Decroly
Médecine/Sciences 34:919-921 (2018)10.1051/medsci/2018230
The methyltransferase domain of the Sudan ebolavirus L protein specifically targets internal adenosines of RNA substrates, in addition to the cap structure
Baptiste Martin, Bruno Coutard, Théo Guez, Guido Paesen, Bruno Canard, Françoise Debart, Jean-Jacques Vasseur, Jonathan Grimes, Etienne Decroly
Nucleic Acids Research 46:7902-7912 (2018)10.1093/nar/gky637
Innate immunity evasion mechanisms of filoviruses
Baptiste Martin, Etienne Decroly
Med Sci (Paris) 34:671-677 (2018)10.1051/medsci/20183408013
Hijacking DNA methyltransferase transition state analogues to produce chemical scaffolds for PRMT inhibitors
Ludovic Halby, Nils Marechal, Dany Pechalrieu, Vincent Cura, Don-Marc Franchini, Céline Faux, Frédéric Alby, Nathalie Troffer-Charlier, Srikanth Kudithipudi, Albert Jeltsch, Wahiba Aouadi, Etienne Decroly, Jean-Claude Guillemot, Patrick Page, Clotilde Ferroud, Luc Bonnefond, Dominique Guianvarc'H, Jean Cavarelli, Paola Arimondo
Philosophical Transactions of the Royal Society B: Biological Sciences 373:20170072 (2018)10.1098/rstb.2017.0072
Filovirus proteins for antiviral drug discovery: Structure/function of proteins involved in assembly and budding
Baptiste Martin, Olivier Reynard, Viktor Volchkov, Etienne Decroly
Antiviral Research 150:183-192 (2018)10.1016/j.antiviral.2017.12.022
Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA
Francois Ferron, Lorenzo Subissi, Ana Theresa Silveira de Morais, Nhung Thi Tuyet Le, Marion Sevajol, Laure Gluais, Etienne Decroly, Clemens Vonrhein, Gérard Bricogne, Bruno Canard, Isabelle Imbert
Proceedings of the National Academy of Sciences of the United States of America 115:E162-E171 (2018)10.1073/pnas.1718806115
2017
Toward the identification of viral cap-methyltransferase inhibitors by fluorescence screening assay
Wahiba Aouadi, C Ecilia Eydoux, Bruno Coutard, Baptiste Martin, Françoise Debart, Jean-Jacques Vasseur, Jean Marie Contreras, Christophe Morice, Gilles Quérat, Marie-Louise Jung, Bruno Canard, Jean-Claude Guillemot, Etienne Decroly
Antiviral Research 144:330-339 (2017)10.1016/j.antiviral.2017.06.021
Biochemical principles and inhibitors to interfere with viral capping pathways
Etienne Decroly, Bruno Canard
Current Opinion in Virology 24:87-96 (2017)10.1016/j.coviro.2017.04.003
Filovirus proteins for antiviral drug discovery: Structure/function bases of the replication cycle
Baptiste Martin, Bruno Canard, Etienne Decroly
Antiviral Research 141:48-61 (2017)10.1016/j.antiviral.2017.02.004
Antiviral activity of [1,2,3]triazolo[4,5- d ]pyrimidin-7(6 H )-ones against chikungunya virus targeting the viral capping nsP1
Alba Gigante, Asier Gómez-Sanjuan, Leen Delang, Changqing Li, Oskía Bueno, Ana-María Gamo, Eva-María Priego, María-José Camarasa, Dirk Jochmans, Pieter Leyssen, Etienne Decroly, Bruno Coutard, Gilles Querat, Johan Neyts, María-Jesús Pérez-Pérez
Antiviral Research 144:216-222 (2017)10.1016/j.antiviral.2017.06.003
Discovery of novel dengue virus NS5 methyltransferase non-nucleoside inhibitors by fragment-based drug design
Fatiha Benmansour, Iuni Trist, Bruno Coutard, Etienne Decroly, Gilles Querat, Andrea Brancale, Karine Barral
European Journal of Medicinal Chemistry 125:865-880 (2017)10.1016/j.ejmech.2016.10.007
Binding of the Methyl Donor S -Adenosyl-l-Methionine to Middle East Respiratory Syndrome Coronavirus 2′- O -Methyltransferase nsp16 Promotes Recruitment of the Allosteric Activator nsp10
Wahiba Aouadi, Alexandre Blanjoie, Jean-Jacques Vasseur, Françoise Debart, Bruno Canard, Etienne Decroly
Journal of Virology 91:1103-1119 (2017)10.1128/JVI.02217-16
Zika Virus Methyltransferase: Structure and Functions for Drug Design Perspectives
Bruno Coutard, Karine Barral, Julie Lichiere, Barbara Selisko, Baptiste Martin, Wahiba Aouadi, Miguel Ortiz Lombardia, Françoise Debart, Jean-Jacques Vasseur, Jean Claude Guillemot, Bruno Canard, Etienne Decroly
Journal of Virology 91:2202-2218 (2017)10.1128/JVI.02202-16
2016
Filovirus proteins for antiviral drug discovery: A structure/function analysis of surface glycoproteins and virus entry
Baptiste Martin, Thomas Hoenen, Bruno Canard, Etienne Decroly
Antiviral Research 135:1-14 (2016)10.1016/j.antiviral.2016.09.001
The methyltransferase domain of dengue virus protein NS5 ensures efficient RNA synthesis initiation and elongation by the polymerase domain (vol 42, pg 11642, 2014)
Supanee Potisopon, Stephane Priet, Axelle Collet, Etienne Decroly, Bruno Canard, Barbara Selisko
Nucleic Acids Research 44:2974 (2016)10.1093/nar/gkv1294
Reevaluation of possible outcomes of infections with human immunodeficiency virus
C. Tamalet, Philippe Colson, Etienne Decroly, C. Dhiver, I. Ravaux, A. Stein, Didier Raoult
Clinical Microbiology and Infection 22:299-311 (2016)10.1016/j.cmi.2015.11.022
The viral capping enzyme nsP1: a novel target for the inhibition of chikungunya virus infection
L. Delang, C. Li, A. Tas, G. Querat, I. C. Albulescu, T. de Burghgraeve, N. A. Segura Guerrero, A. Gigante, G. Piorkowski, Etienne Decroly, D. Jochmans, B. Canard, E. J. Snijder, M. J. Perez-Perez, M. J. Van Hemert, B. Coutard, P. Leyssen, J. Neyts
Scientific Reports 6:31819 (2016)10.1038/srep31819
Involvement of an Arginine Triplet in M1 Matrix Protein Interaction with Membranes and in M1 Recruitment into Virus-Like Particles of the Influenza A(H1N1)pdm09 Virus
Adeline Kerviel, Shantoshini Dash, Olivier Moncorgé, Baptiste Panthu, Jan Prchal, Didier Decimo, Théophile Ohlmann, Bruno Lina, Cyril Favard, Etienne Decroly, Michèle Ottmann, Philippe Roingeard, Delphine Muriaux
PLoS ONE 11:e0165421 (2016)10.1371/journal.pone.0165421
2015
mRNA Capping by Venezuelan Equine Encephalitis Virus nsP1: Functional Characterization and Implications for Antiviral Research
Changqing Li, Jaime Guillen, Nadia Rabah, Alexandre Blanjoie, Francoise Debart, Jean-Jacques Vasseur, Bruno Canard, Etienne Decroly, Bruno Coutard
Journal of Virology 89:8292-8303 (2015)10.1128/JVI.00599-15
X-ray structure and activities of an essential Mononegavirales L-protein domain
Guido C. Paesen, Axelle Collet, Corinne Sallamand, Francoise Debart, Jean-Jacques Vasseur, Bruno Canard, Etienne Decroly, Jonathan M. Grimes
Nature Communications 6:8749 (2015)10.1038/ncomms9749
2014
Insights into RNA synthesis, capping, and proofreading mechanisms of SARS-coronavirus
Marion Sevajol, Lorenzo Subissi, Etienne Decroly, Bruno Canard, Isabelle Imbert
Virus Research 194:90-99 (2014)10.1016/j.virusres.2014.10.008
The methyltransferase domain of dengue virus protein NS5 ensures efficient RNA synthesis initiation and elongation by the polymerase domain
Supanee Potisopon, Stéphane Priet, Axelle Collet, Etienne Decroly, Bruno Canard, Barbara Selisko
Nucleic Acids Research 42 (2014)10.1093/nar/gku666
One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities
Lorenzo Subissi, Clara Posthuma, Axelle Collet, Jessika Zevenhoven-Dobbe, Alexander Gorbalenya, Etienne Decroly, Eric Snijder, Bruno Canard, Isabelle Imbert
Proceedings of the National Academy of Sciences of the United States of America 111 (2014)10.1073/pnas.1323705111
Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes
Mickaël Bouvet, Adrien Lugari, Clara C. Posthuma, Jessika C. Zevenhoven, Stéphanie Bernard, Stéphane Betzi, Isabelle Imbert, Bruno Canard, Jean-Claude Guillemot, Patrick Lécine, Susanne Pfefferle, Christian Drosten, Eric J. Snijder, Etienne Decroly, Xavier Morelli
Journal of Biological Chemistry 289:25783-25796 (2014)10.1074/jbc.M114.577353
Assessment of Dengue virus helicase and methyltransferase as targets for fragment-based drug discovery
Bruno Coutard, Etienne Decroly, Changqing Li, Andrew Sharff, Julien Lescar, Gérard Bricogne, Karine Barral
Antiviral Research 106:61-70 (2014)10.1016/j.antiviral.2014.03.013
2013
Development of specific dengue virus 2'-O- and N7-methyltransferase assays for antiviral drug screening.
Karine Barral, Corinne Sallamand, Christiane Petzold, Bruno Coutard, Axelle Collet, Yann Thillier, Jan Zimmermann, Jean-Jacques Vasseur, Bruno Canard, Jacques Rohayem, Françoise Debart, Etienne Decroly
Antiviral Research 99:292-300 (2013)10.1016/j.antiviral.2013.06.001
2012
Synthesis of 5' cap-0 and cap-1 RNAs using solid-phase chemistry coupled with enzymatic methylation by human (guanine-N 7)-methyl transferase
Yann Thillier, Etienne Decroly, François Morvan, Bruno Canard, Jean-Jacques Vasseur, Françoise Debart
RNA 18:856-868 (2012)10.1261/rna.030932.111
2011
Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex.
Claire Debarnot, Isabelle Imbert, Francois Ferron, Laure Gluais, Isabelle Varlet, Nicolas Papageorgiou, Mickaël Bouvet, Julien Lescar, Etienne Decroly, Bruno Canard
Acta crystallographica Section F : Structural biology communications [2014-...] 67:404-8 (2011)10.1107/S1744309111002867
2007
High-yield production of short GppA- and 7Me GppA-capped RNAs and HPLC-monitoring of methyltransfer reactions at the guanine-N7 and adenosine-2'O positions.
F. Peyrane, B. Selisko, Etienne Decroly, J.-J. Vasseur, D. Benarroch, B. Canard, K. Alvarez
Nucleic Acids Research 35:1-11 (2007)
2001
Interaction of human immunodeficiency virus type 1 Vif with Gag and Gag-Pol precursors : co-encapsidation and interference with viral protease-mediated Gag processing
Martine Bardy, Bernard Gay, Stéphanie Pébernard, Nathalie Chazal, Marianne Courcoul, Robert Vigne, Etienne Decroly, Pierre Boulanger
Journal of General Virology 82:2719-2733 (2001)10.1099/0022-1317-82-11-2719
2000
An Anti-Human Immunodeficiency Virus Multiple Antigen Peptide Encompassing the Cleavage Region of the Env Precursor Interferes With Membrane Fusion at a Post-CD4 Binding Step
Rym Barbouche, Etienne Decroly, Marie Paule Kieny, Emmanuel Fenouillet
Virology 273:169-177 (2000)10.1006/viro.2000.0368
