Jean-Claude Guillemot
Professor
Research group: Viral Replicases: Structure, function and drug-design
Facility: Screening Platform Marseille-Luminy (PCML)
JC Guillemot obtained a PhD in 1995 in Hematology from the University of Toulouse at the Beth Israel Hospital, Harvard Medical School (Boston-USA). His HDR, in 2002, from the University of Provence, was on the “Role of GTPases”, in innate immune functions. His MBA, in 2015, Management of Innovation, at IAE-AMU was focused on the strategic development of antivirals against the Dengue Virus. He is currently the head of the PCML platform. Dedicated to the search of antivirals, innovative approaches are developed to identify new targets, mechanisms based on HTS screenings, with a focus on emerging viruses.
Publications
2023
Single-cell RNA-sequencing of PBMCs from SAVI patients reveals disease-associated monocytes with elevated integrated stress response
Camille de Cevins, Laure Delage, Maxime Batignes, Quentin Riller, Marine Luka, Anne Remaury, Boris Sorin, Tinhinane Fali, Cécile Masson, Bénédicte Hoareau, Catherine Meunier, Mélanie Parisot, Mohammed Zarhrate, Brieuc P Pérot, Víctor García-Paredes, Francesco Carbone, Lou Galliot, Béatrice Nal, Philippe Pierre, Luc Canard, Charlotte Boussard, Etienne Crickx, Jean-Claude Guillemot, Brigitte Bader-Meunier, Alexandre Bélot, Pierre Quartier, Marie-Louise Frémond, Bénédicte Neven, Galina Boldina, Franck Augé, Fischer Alain, Michel Didier, Frédéric Rieux-Laucat, Mickaël M Ménager
Cell Reports Medicine 4:101333 (2023)10.1016/j.xcrm.2023.101333
Notch activation shifts the fate decision of senescent progenitors toward myofibrogenesis in human adipose tissue
Nathalie Boulet, Anaïs Briot, Valentin Jargaud, David Estève, Anne Rémaury, Chloé Belles, Pénélope Viana, Jessica Fontaine, Lucie Murphy, Catherine Déon, Marie Guillemot, Catherine Pech, Yaligara Veeranagouda, Michel Didier, Pauline Decaunes, Etienne Mouisel, Christian Carpéné, Jason S Iacovoni, Alexia Zakaroff-Girard, Jean-Louis Grolleau, Jean Galitzky, Séverine Ledoux, Jean-Claude Guillemot, Anne Bouloumié
Aging Cell e13776 (2023)10.1111/acel.13776
AT-752 targets multiple sites and activities on the Dengue virus replication enzyme NS5
Mikael Feracci, Cécilia Eydoux, Véronique Fattorini, Lea Lo Bello, Pierre Gauffre, Barbara Selisko, Priscila Sutto-Ortiz, Ashleigh Shannon, Hongjie Xia, Pei-Yong Shi, Mathieu Noel, Françoise Debart, Jean-Jacques Vasseur, Steve Good, Kai Lin, Adel Moussa, Jean-Pierre Sommadossi, Aurélie Chazot, Karine Alvarez, Jean-Claude Guillemot, Etienne Decroly, François Ferron, Bruno Canard
Antiviral Research 212:105574 (2023)10.1016/j.antiviral.2023.105574
2022
A Versatile Class of 1,4,4-Trisubstituted Piperidines Block Coronavirus Replication In Vitro
Sonia de Castro, Annelies Stevaert, Miguel Maldonado, Adrien Delpal, Julie Vandeput, Benjamin van Loy, Cecilia Eydoux, Jean-Claude Guillemot, Etienne Decroly, Federico Gago, Bruno Canard, Maria-Jose Camarasa, Sonsoles Velázquez, Lieve Naesens
Pharmaceuticals 15:1021 (2022)10.3390/ph15081021
A dual mechanism of action of AT-527 against SARS-CoV-2 polymerase
Ashleigh Shannon, Véronique Fattorini, Bhawna Sama, Barbara Selisko, Mikael Feracci, Camille Falcou, Pierre Gauffre, Priscila El Kazzi, Adrien Delpal, Etienne Decroly, Karine Alvarez, Cécilia Eydoux, Jean-Claude Guillemot, Adel Moussa, Steven S Good, Paolo La Colla, Kai Lin, Jean-Pierre Sommadossi, Yingxiao Zhu, Xiaodong Yan, Hui Shi, Francois Ferron, Bruno Canard
Nature Communications 13 (2022)10.1038/s41467-022-28113-1
Synthesis, Structure–Activity Relationships, and Antiviral Profiling of 1-Heteroaryl-2-Alkoxyphenyl Analogs As Inhibitors of SARS-CoV-2 Replication
Dorothée Bardiot, Laura Vangeel, Mohamed Koukni, Philippe Arzel, Marleen Zwaagstra, Heyrhyoung Lyoo, Patrick Wanningen, Shamshad Ahmad, Linlin Zhang, Xinyuanyuan Sun, Adrien Delpal, Cecilia Eydoux, Jean-Claude Guillemot, Eveline Lescrinier, Hugo Klaassen, Pieter Leyssen, Dirk Jochmans, Karolien Castermans, Rolf Hilgenfeld, Colin Robinson, Etienne Decroly, Bruno Canard, Eric J Snijder, Martijn J Van Hemert, Frank Van van Kuppeveld, Patrick Chaltin, Johan Neyts, Steven De de Jonghe, Arnaud Marchand
Molecules 27:1052 (2022)10.3390/molecules27031052
2021
Simeprevir Potently Suppresses SARS-CoV-2 Replication and Synergizes with Remdesivir
Ho Sing Lo, Kenrie Pui Yan Hui, Hei-Ming Lai, Xu He, Khadija Shahed Khan, Simranjeet Kaur, Junzhe Huang, Zhongqi Li, Anthony Chan, Hayley Hei-Yin Cheung, Ka-Chun Ng, John Chi Wang Ho, Yu Wai Chen, Bowen Ma, Peter Man-Hin Cheung, Donghyuk Shin, Kaidao Wang, Meng-Hsuan Lee, Barbara Selisko, Cecilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Kuen-Phon Wu, Po-Huang Liang, Ivan Dikic, Zhong Zuo, Francis Chan, David Hui, Vincent Mok, Kam-Bo Wong, Chris Ka Pun Mok, Ho Ko, Wei Shen Aik, Michael Chi Wai Chan, Wai-Lung Ng
ACS Central Science 7:792-802 (2021)10.1021/acscentsci.0c01186
A fluorescence-based high throughput-screening assay for the SARS-CoV RNA synthesis complex
Cecilia Eydoux, Veronique Fattorini, Ashleigh Shannon, Thi-Tuyet-Nhung Le, Bruno Didier, Bruno Canard, Jean-Claude Guillemot
Journal of Virological Methods 288:114013 (2021)10.1016/j.jviromet.2020.114013
2020
Drugs against SARS‐CoV ‐2: What do we know about their mode of action?
Coralie Valle, Baptiste Martin, Franck Touret, Ashleigh Shannon, Bruno Canard, Jean‐claude Guillemot, Bruno Coutard, Etienne Decroly
Reviews in Medical Virology 30:1-10 (2020)10.1002/rmv.2143
Obesity of mice lacking VAP-1/SSAO by Aoc3 gene deletion is reproduced in mice expressing a mutated vascular adhesion protein-1 (VAP-1) devoid of amine oxidase activity
Valentin Jargaud, Sandy Bour, François Tercé, Xavier Collet, Philippe Valet, Anne Bouloumié, Jean-Claude Guillemot, Pascale Mauriège, Sirpa Jalkanen, Craig Stolen, Marko Salmi, David Smith, Christian Carpéné
Journal of Physiology and Biochemistry 77:141-154 (2020)10.1007/s13105-020-00756-y
Remdesivir and SARS-CoV-2: Structural requirements at both nsp12 RdRp and nsp14 Exonuclease active-sites
Ashleigh Shannon, Nhung Thi-Tuyet Le, Barbara Selisko, Cecilia Eydoux, Karine Alvarez, Jean-Claude Guillemot, Etienne Decroly, Olve Peersen, Francois Ferron, Bruno Canard
Antiviral Research 178:104793 (2020)10.1016/j.antiviral.2020.104793
Incretin combination therapy for the treatment of non-alcoholic steatohepatitis
Aimo Kannt, Andreas Nygaard Madsen, Claire Kammermeier, Ralf Elvert, Tim Klöckener, Martin Bossart, Torsten Haack, Andreas Evers, Katrin Lorenz, W. Hennerici, Corinne Rocher, Zsolt Böcskei, Jean-Claude Guillemot, Vincent Mikol, François Pattou, Bart Staels, Michael Wagner
Diabetes, Obesity and Metabolism Online ahead of print. (2020)10.1111/dom.14035
Carneic Acids from an Endophytic Phomopsis sp. as Dengue Virus Polymerase Inhibitors
Laure-Anne Peyrat, Véronique Eparvier, Cecilia Eydoux, Jean-Claude Guillemot, Marc Litaudon, Didier Stien
Journal of Natural Products 83:2330-2336 (2020)10.1021/acs.jnatprod.9b01169
2019
Antiviral Compounds from Codiaeum peltatum Targeted by a Multi-informative Molecular Networks Approach
Florent Olivon, Simon Remy, Gwendal Grelier, Cécile Apel, Cecilia Eydoux, Jean-Claude Guillemot, Johan Neyts, Leen Delang, David Touboul, Fanny Roussi, Marc Litaudon
Journal of Natural Products 82:330-340 (2019)10.1021/acs.jnatprod.8b00800
Approved drugs screening against the nsP1 capping enzyme of Venezuelan equine encephalitis virus using an immuno-based assay
Ana Ferreira-Ramos, Changqing Li, Cecilia Eydoux, Jean Marie Contreras, Christophe Morice, Gilles Querat, Alba Gigante, María-Jesús Pérez Pérez, Marie-Louise Jung, Bruno Canard, Jean-Claude Guillemot, Etienne Decroly, Bruno Coutard
Antiviral Research 163:59-69 (2019)10.1016/j.antiviral.2019.01.003
2018
Hijacking DNA methyltransferase transition state analogues to produce chemical scaffolds for PRMT inhibitors
Ludovic Halby, Nils Marechal, Dany Pechalrieu, Vincent Cura, Don-Marc Franchini, Céline Faux, Frédéric Alby, Nathalie Troffer-Charlier, Srikanth Kudithipudi, Albert Jeltsch, Wahiba Aouadi, Etienne Decroly, Jean-Claude Guillemot, Patrick Page, Clotilde Ferroud, Luc Bonnefond, Dominique Guianvarc'H, Jean Cavarelli, Paola Arimondo
Philosophical Transactions of the Royal Society B: Biological Sciences 373:20170072 (2018)10.1098/rstb.2017.0072
2017
Toward the identification of viral cap-methyltransferase inhibitors by fluorescence screening assay
Wahiba Aouadi, C Ecilia Eydoux, Bruno Coutard, Baptiste Martin, Françoise Debart, Jean-Jacques Vasseur, Jean Marie Contreras, Christophe Morice, Gilles Quérat, Marie-Louise Jung, Bruno Canard, Jean-Claude Guillemot, Etienne Decroly
Antiviral Research 144:330-339 (2017)10.1016/j.antiviral.2017.06.021
NF-L in cerebrospinal fluid and serum is a biomarker of neuronal damage in an inducible mouse model of neurodegeneration
Anthony Brureau, Véronique Blanchard-Bregeon, Catherine Pech, Stéphanie Hamon, Pascal Chaillou, Jean-Claude Guillemot, Pascal Barneoud, Philippe Bertrand, Laurent Pradier, Thomas Rooney, Nathalie Schussler
Neurobiology of Disease 104:73-84 (2017)10.1016/j.nbd.2017.04.007
Zika Virus Methyltransferase: Structure and Functions for Drug Design Perspectives
Bruno Coutard, Karine Barral, Julie Lichiere, Barbara Selisko, Baptiste Martin, Wahiba Aouadi, Miguel Ortiz Lombardia, Françoise Debart, Jean-Jacques Vasseur, Jean Claude Guillemot, Bruno Canard, Etienne Decroly
Journal of Virology 91:2202-2218 (2017)10.1128/JVI.02202-16
2016
Betulinic Acid, The First Lupane-Type Triterpenoid Isolated from Both a Phomopsis sp. and Its Host Plant Diospyros carbonaria Benoist
Laure-Anne Peyrat, Véronique Eparvier, Cécilia Eydoux, Jean-Claude Guillemot, Marc Litaudon, Didier Stien
Chemistry and Biodiversity 14:e1600171 (2016)10.1002/cbdv.201600171
Protein-Protein Interaction Inhibition (2P21)-Oriented Chemical Library Accelerates Hit Discovery
Sabine Milhas, Brigitt Raux, Stéphane Betzi, Carine Derviaux, Philippe Roche, Audrey Restouin, Marie-Jeanne Basse, Etienne Rebuffet, Adrien Lugari, Marion Badol, Rudra Kashyap, Jean-Claude Lissitzky, Cecilia Eydoux, Veronique Hamon, Marie -Edith Gourde, Sebastien Combes, Pascale Zimmermann, Michel Aurrand-Lions, Thomas Roux, Catherine Rogers, Susanne Muller, Stefan Knapp, Eric Trinquet, Yves Collette, Jean-Claude Guillemot, Xavier Morelli
ACS Chemical Biology 11:2140-2148 (2016)10.1021/acschembio.6b00286
Exploring Selective Inhibition of the First Bromodomain of the Human Bromodomain and Extra-terminal Domain (BET) Proteins
Brigitt Raux, Yuliia Voitovich, Carine Derviaux, Adrien Lugari, Etienne Rebuffet, Sabine Milhas, Stephane Priet, Thomas Roux, Eric Trinquet, Jean-Claude Guillemot, Stefan Knapp, Jean-Michel Brunel, Alexey Yu. Fedorov, Yves Collette, Philippe Roche, Stéphane Betzi, Sebastien Combes, Xavier Morelli
Journal of Medicinal Chemistry 59:1634-1641 (2016)10.1021/acs.jmedchem.5b01708
Novel 2-phenyl-5-[(E)-2-(thiophen-2-yl)ethenyl]-1,3,4-oxadiazole and 3-phenyl-5-[(E)-2-(thiophen-2-yl)ethenyl]-1,2,4-oxadiazole derivatives as dengue virus inhibitors targeting NS5 polymerase
Fatiha Benmansour, Cecilia Eydoux, Gilles Querat, Xavier de Lamballerie, Bruno Canard, Karine Alvarez, Jean-Claude Guillemot, Karine Barral
European Journal of Medicinal Chemistry 109:146-156 (2016)10.1016/j.ejmech.2015.12.046
Chemical diversity and antiviral potential in the pantropical Diospyros genus
Laure-Anne Peyrat, Véronique Eparvier, Cécilia Eydoux, Jean-Claude Guillemot, Didier Stien, Marc Litaudon
Fitoterapia (2016)10.1016/j.fitote.2016.04.017
2015
Les années 2010
Michel Bouvier, Herve Chneiweiss, Bertrand Jordan, Gilbert Lagrue, Joël Ménard, Jean-Luc Teillaud, Guido Kroemer, Anne-Marie Moulin, Antoine Flahault, Laurence Lafanechère, Eric Maréchal, Jean-Claude Guillemot, Marie Gaille
Médecine/Sciences 31:91-111 (2015)10.1051/medsci/201531s1006
2014
La chémobiologie : stratégies transfrontalières
Laurence Lafanechère, Eric Maréchal, Jean-Claude Guillemot
Médecine/Sciences 30:2 (2014)10.1051/medsci/20143012001
Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes
Mickaël Bouvet, Adrien Lugari, Clara C. Posthuma, Jessika C. Zevenhoven, Stéphanie Bernard, Stéphane Betzi, Isabelle Imbert, Bruno Canard, Jean-Claude Guillemot, Patrick Lécine, Susanne Pfefferle, Christian Drosten, Eric J. Snijder, Etienne Decroly, Xavier Morelli
Journal of Biological Chemistry 289:25783-25796 (2014)10.1074/jbc.M114.577353
2013
Plasma Peptide Biomarker Discovery for Amyotrophic Lateral Sclerosis by MALDI –TOF Mass Spectrometry Profiling
Laurence Conraux, Catherine Pech, Halim Guerraoui, Denis Loyaux, Pascual Ferrara, Jean-Claude Guillemot, Vincent Meininger, Pierre-François Pradat, François Salachas, Gaelle Bruneteau, Nadine Le Forestier, Lucette Lacomblez
PLoS ONE 8:e79733 (2013)10.1371/journal.pone.0079733
2012
Antiviral chlorinated daphnane diterpenoid orthoesters from the bark and wood of Trigonostemon cherrieri.
Pierre-Marie Allard, Pieter Leyssen, Marie-Thérèse Martin, Mélanie Bourjot, Vincent Dumontet, Cécilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Cyril Poullain, Françoise Guéritte, Marc Litaudon
Phytochemistry 84:160-8 (2012)10.1016/j.phytochem.2012.07.023
Chemical constituents of Anacolosa pervilleana and their antiviral activities.
Mélanie Bourjot, Pieter Leyssen, Cécilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Philippe Rasoanaivo, Françoise Guéritte, Marc Litaudon
Fitoterapia 83:1076-1080 (2012)10.1016/j.fitote.2012.05.004
Flacourtosides A-F, Phenolic Glycosides Isolated from Flacourtia ramontchi.
Mélanie Bourjot, Pieter Leyssen, Cécilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Philippe Rasoanaivo, Françoise Guéritte, Marc Litaudon
Journal of Natural Products 75:752-8 (2012)10.1021/np300059n
2011
Alkylated flavanones from the bark of Cryptocarya chartacea as dengue virus NS5 polymerase inhibitors.
Pierre-Marie Allard, Elise Tran Huu Dau, Cécilia Eydoux, Jean-Claude Guillemot, Vincent Dumontet, Cyril Poullain, Bruno Canard, Françoise Guéritte, Marc Litaudon
Journal of Natural Products 74:2446-53 (2011)10.1021/np200715v
PHYTOCHIK: Biodiversity As A Source of Selective Inhibitors of CHIKV Replication
Pieter Leyssen, Marc Litaudon, Jean-Claude Guillemot, Philippe Rasoanaivo, Jacqueline Smadja, Ameenah Gurib-Fakim, Bruno Canard, Françoise Guéritte
Antiviral Research 90:A36-A37 (2011)10.1016/j.antiviral.2011.03.048
2009
Identification of allosteric inhibitors blocking the hepatitis C virus polymerase NS5B in the RNA synthesis initiation step.
Stéphane Betzi, Cécilia Eydoux, Cécile Bussetta, Marilyne Blemont, Pieter Leyssen, Claire Debarnot, Mohamed Ben-Rahou, Jacques Haiech, Marcel Hibert, Françoise Gueritte, David S. Grierson, Jean-Louis Romette, Jean-Claude Guillemot, Johan Neyts, Karine Alvarez, Xavier Morelli, Hélène Dutartre, Bruno Canard
Antiviral Research 84:48-59 (2009)10.1016/j.antiviral.2009.06.009
2006
Comparative mechanistic studies of de novo RNA synthesis by flavivirus RNA-dependent RNA polymerases
Barbara Selisko, Hélène Dutartre, Jean-Claude Guillemot, Claire Debarnot, Delphine Benarroch, Alexander Khromykh, Philippe Desprès, Marie-Pierre Egloff, Bruno Canard
Virology 351:145-158 (2006)10.1016/j.virol.2006.03.026
2005
A Relaxed Discrimination of 2′- O -Methyl-GTP Relative to GTP between de Novo and Elongative RNA Synthesis by the Hepatitis C RNA-dependent RNA Polymerase NS5B
Hélène Dutartre, Joëlle Boretto, Jean Claude Guillemot, Bruno Canard
Journal of Biological Chemistry 280:6359-6368 (2005)10.1074/jbc.M410191200
2002
Identification of two tamoxifen target proteins by photolabeling with 4-(2-morpholinoethoxy)benzophenone.
Fabienne Mésange, Mohamed Sebbar, Joël Capdevielle, Jean-Claude Guillemot, Pascual Ferrara, Francis Bayard, Marc E. Poirot, Jean-Charles Faye
Bioconjug Chem 13:766-72 (2002)
Porcine odorant-binding protein selectively binds to a human olfactory receptor
Valéry Matarazzo, Nicole Zsürger, Jean-Claude Guillemot, Olivier Clot-Faybesse, Jean-Marie Botto, Claude Dal Farra, Marc Crowe, Jacques Demaille, Jean-Pierre Vincent, Jean Mazella, Catherine Ronin
Chemical Senses 27:691-701 (2002)10.1093/chemse/27.8.691
1998
Tyrosine phosphorylation of the Wiskott-Aldrich Syndrome protein by Lyn and Btk is regulated by CDC42
Rodolphe r. Guinamard, Pontus Aspenström, Michel Fougereau, Philippe Chavrier, Jean-Claude Guillemot
FEBS Letters 434:431-436 (1998)10.1016/s0014-5793(98)01016-3
Microsomal epoxide hydrolase of rat liver is a subunit of the anti-oestrogen-binding site.
Fabienne Mésange, Mohamed Sebbar, Blandine Kedjouar, Joël Capdevielle, Jean-Claude Guillemot, Pascual Ferrara, Francis Bayard, Frédéric Delarue, Jean-Charles Faye, Marc E. Poirot
Biochemical Journal 334 ( Pt 1):107-12 (1998)
