Facility: Screening Platform Marseille-Luminy (PCML)

JC Guillemot obtained a PhD in 1995 in Hematology from the University of Toulouse at the Beth Israel Hospital, Harvard Medical School (Boston-USA). His HDR, in 2002, from the University of Provence, was on the “Role of GTPases”, in innate immune functions. His MBA, in 2015, Management of Innovation, at IAE-AMU was focused on the strategic development of antivirals against the Dengue Virus. He is currently the head of the PCML platform. Dedicated to the search of antivirals, innovative approaches are developed to identify new targets, mechanisms based on HTS screenings, with a focus on emerging viruses.

Publications

2022

2022
A dual mechanism of action of AT-527 against SARS-CoV-2 polymerase

Ashleigh Shannon, Véronique Fattorini, Bhawna Sama, Barbara Selisko, Mikael Feracci, Camille Falcou, Pierre Gauffre, Priscila El Kazzi, Adrien Delpal, Etienne Decroly, Karine Alvarez, Cécilia Eydoux, Jean-Claude Guillemot, Adel Moussa, Steven S Good, Paolo La Colla, Kai Lin, Jean-Pierre Sommadossi, Yingxiao Zhu, Xiaodong Yan, Hui Shi, Francois Ferron, Bruno Canard

Nature Communications 13 (2022)10.1038/s41467-022-28113-1

2022
Synthesis, Structure–Activity Relationships, and Antiviral Profiling of 1-Heteroaryl-2-Alkoxyphenyl Analogs As Inhibitors of SARS-CoV-2 Replication

Dorothée Bardiot, Laura Vangeel, Mohamed Koukni, Philippe Arzel, Marleen Zwaagstra, Heyrhyoung Lyoo, Patrick Wanningen, Shamshad Ahmad, Linlin Zhang, Xinyuanyuan Sun, Adrien Delpal, Cecilia Eydoux, Jean-Claude Guillemot, Eveline Lescrinier, Hugo Klaassen, Pieter Leyssen, Dirk Jochmans, Karolien Castermans, Rolf Hilgenfeld, Colin Robinson, Etienne Decroly, Bruno Canard, Eric J Snijder, Martijn J Van Hemert, Frank Van van Kuppeveld, Patrick Chaltin, Johan Neyts, Steven De de Jonghe, Arnaud Marchand

Molecules 27:1052 (2022)10.3390/molecules27031052

2021

2021
Simeprevir Potently Suppresses SARS-CoV-2 Replication and Synergizes with Remdesivir

Ho Sing Lo, Kenrie Pui yan Hui, Hei-Ming Lai, Xu He, Khadija Shahed Khan, Simranjeet Kaur, Junzhe Huang, Zhongqi Li, Anthony Chan, Hayley Hei-yin Cheung, Ka-Chun Ng, John Chi Wang Ho, yu Wai Chen, Bowen Ma, Peter Man-Hin Cheung, Donghyuk Shin, Kaidao Wang, Meng-Hsuan Lee, Barbara Selisko, Cecilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Kuen-Phon Wu, Po-Huang Liang, Ivan Dikic, Zhong Zuo, Francis Chan, David Hui, Vincent Mok, Kam-Bo Wong, Chris Ka Pun Mok, Ho Ko, Wei Shen Aik, Michael Chi Wai Chan, Wai-Lung Ng

ACS Central Science 7:792-802 (2021)10.1021/acscentsci.0c01186

2021
A fluorescence-based high throughput-screening assay for the SARS-CoV RNA synthesis complex

Cecilia Eydoux, Veronique Fattorini, Ashleigh Shannon, Thi-Tuyet-Nhung Le, Bruno Didier, Bruno Canard, Jean-Claude Guillemot

Journal of Virological Methods 288:114013 (2021)10.1016/j.jviromet.2020.114013

2020

2020
Drugs against SARS‐CoV ‐2: What do we know about their mode of action?

Coralie Valle, Baptiste Martin, Franck Touret, Ashleigh Shannon, Bruno Canard, Jean‐claude Guillemot, Bruno Coutard, Etienne Decroly

Reviews in Medical Virology 30:1-10 (2020)10.1002/rmv.2143

2020
Remdesivir and SARS-CoV-2: Structural requirements at both nsp12 RdRp and nsp14 Exonuclease active-sites

Ashleigh Shannon, Nhung Thi-Tuyet Le, Barbara Selisko, Cecilia Eydoux, Karine Alvarez, Jean-Claude Guillemot, Etienne Decroly, Olve Peersen, Francois Ferron, Bruno Canard

Antiviral Research 178:104793 (2020)10.1016/j.antiviral.2020.104793

2020
Incretin combination therapy for the treatment of non-alcoholic steatohepatitis

Aimo Kannt, Andreas Nygaard Madsen, Claire Kammermeier, Ralf Elvert, Tim Klöckener, Martin Bossart, Torsten Haack, Andreas Evers, Katrin Lorenz, W. Hennerici, Corinne Rocher, Zsolt Böcskei, Jean-Claude Guillemot, Vincent Mikol, François Pattou, Bart Staels, Michael Wagner

Diabetes, Obesity and Metabolism Online ahead of print. (2020)10.1111/dom.14035

2020
Carneic Acids from an Endophytic Phomopsis sp. as Dengue Virus Polymerase Inhibitors

Laure-Anne Peyrat, Véronique Eparvier, Cecilia Eydoux, Jean-Claude Guillemot, Marc Litaudon, Didier Stien

Journal of Natural Products (2020)10.1021/acs.jnatprod.9b01169

2019

2019
Antiviral Compounds from Codiaeum peltatum Targeted by a Multi-informative Molecular Networks Approach

Florent Olivon, Simon Remy, Gwendal Grelier, Cécile Apel, Cecilia Eydoux, Jean-Claude Guillemot, Johan Neyts, Leen Delang, David Touboul, Fanny Roussi, Marc Litaudon

Journal of Natural Products 82:330-340 (2019)10.1021/acs.jnatprod.8b00800

2019
Approved drugs screening against the nsP1 capping enzyme of Venezuelan equine encephalitis virus using an immuno-based assay

Ana Ferreira-Ramos, Changqing Li, Cecilia Eydoux, Jean Marie Contreras, Christophe Morice, Gilles Querat, Alba Gigante, María-Jesús Pérez Pérez, Marie-Louise Jung, Bruno Canard, Jean-Claude Guillemot, Etienne Decroly, Bruno Coutard

Antiviral Research 163:59-69 (2019)10.1016/j.antiviral.2019.01.003

2018

2018
Hijacking DNA methyltransferase transition state analogues to produce chemical scaffolds for PRMT inhibitors

Ludovic Halby, Nils Marechal, Dany Pechalrieu, Vincent Cura, Don-Marc Franchini, Céline Faux, Frédéric Alby, Nathalie Troffer-Charlier, Srikanth Kudithipudi, Albert Jeltsch, Wahiba Aouadi, Etienne Decroly, Jean-Claude Guillemot, Patrick Page, Clotilde Ferroud, Luc Bonnefond, Dominique Guianvarc'H, Jean Cavarelli, Paola Arimondo

Philosophical Transactions of the Royal Society B: Biological Sciences 373:20170072 (2018)10.1098/rstb.2017.0072

2017

2017
NF-L in cerebrospinal fluid and serum is a biomarker of neuronal damage in an inducible mouse model of neurodegeneration

Anthony Brureau, Véronique Blanchard-Bregeon, Catherine Pech, Stéphanie Hamon, Pascal Chaillou, Jean-Claude Guillemot, Pascal Barneoud, Philippe Bertrand, Laurent Pradier, Thomas Rooney, Nathalie Schussler

Neurobiology of Disease 104:73-84 (2017)10.1016/j.nbd.2017.04.007

2017
Toward the identification of viral cap-methyltransferase inhibitors by fluorescence screening assay

Wahiba Aouadi, C Ecilia Eydoux, Bruno Coutard, Baptiste Martin, Françoise Debart, Jean-Jacques Vasseur, Jean Marie Contreras, Christophe Morice, Gilles Quérat, Marie-Louise Jung, Bruno Canard, Jean-Claude Guillemot, Etienne Decroly

Antiviral Research 144:330-339 (2017)10.1016/j.antiviral.2017.06.021

2017
Betulinic Acid, The First Lupane-Type Triterpenoid Isolated from Both a Phomopsis sp. and Its Host Plant Diospyros carbonaria Benoist

Laure-Anne Peyrat, Véronique Eparvier, Cécilia Eydoux, Jean-Claude Guillemot, Marc Litaudon, Didier Stien

Chemistry & Biodiversity 14:e1600171 (2017)10.1002/cbdv.201600171

2017
Zika Virus Methyltransferase: Structure and Functions for Drug Design Perspectives

Bruno Coutard, Karine Barral, Julie Lichiere, Barbara Selisko, Baptiste Martin, Wahiba Aouadi, Miguel Ortiz Lombardia, Françoise Debart, Jean-Jacques Vasseur, Jean Claude Guillemot, Bruno Canard, Etienne Decroly

Journal of Virology 91:2202-2218 (2017)10.1128/JVI.02202-16

2016

2016
Protein-Protein Interaction Inhibition (2P21)-Oriented Chemical Library Accelerates Hit Discovery

Sabine Milhas, Brigitt Raux, Stéphane Betzi, Carine Derviaux, Philippe Roche, Audrey Restouin, Marie-Jeanne Basse, Etienne Rebuffet, Adrien Lugari, Marion Badol, Rudra Kashyap, Jean-Claude Lissitzky, Cecilia Eydoux, Veronique Hamon, Marie -Edith Gourde, Sebastien Combes, Pascale Zimmermann, Michel Aurrand-Lions, Thomas Roux, Catherine Rogers, Susanne Muller, Stefan Knapp, Eric Trinquet, Yves Collette, Jean-Claude Guillemot, Xavier Morelli

ACS Chemical Biology 11:2140-2148 (2016)10.1021/acschembio.6b00286

2016
Exploring Selective Inhibition of the First Bromodomain of the Human Bromodomain and Extra-terminal Domain (BET) Proteins

Brigitt Raux, Yuliia Voitovich, Carine Derviaux, Adrien Lugari, Etienne Rebuffet, Sabine Milhas, Stephane Priet, Thomas Roux, Eric Trinquet, Jean-Claude Guillemot, Stefan Knapp, Jean-Michel Brunel, Alexey Yu. Fedorov, Yves Collette, Philippe Roche, Stéphane Betzi, Sebastien Combes, Xavier Morelli

Journal of Medicinal Chemistry 59:1634-1641 (2016)10.1021/acs.jmedchem.5b01708

2016
Novel 2-phenyl-5-[(E)-2-(thiophen-2-yl)ethenyl]-1,3,4-oxadiazole and 3-phenyl-5-[(E)-2-(thiophen-2-yl)ethenyl]-1,2,4-oxadiazole derivatives as dengue virus inhibitors targeting NS5 polymerase

Fatiha Benmansour, Cecilia Eydoux, Gilles Querat, Xavier de Lamballerie, Bruno Canard, Karine Alvarez, Jean-Claude Guillemot, Karine Barral

European Journal of Medicinal Chemistry 109:146-156 (2016)10.1016/j.ejmech.2015.12.046

2016
Chemical diversity and antiviral potential in the pantropical Diospyros genus

Laure-Anne Peyrat, Véronique Eparvier, Cécilia Eydoux, Jean-Claude Guillemot, Didier Stien, Marc Litaudon

Fitoterapia (2016)10.1016/j.fitote.2016.04.017

2015

2015
Les années 2010

Michel Bouvier, Herve Chneiweiss, Bertrand Jordan, Gilbert Lagrue, Joël Ménard, Jean-Luc Teillaud, Guido Kroemer, Anne-Marie Moulin, Antoine Flahault, Laurence Lafanechère, Eric Maréchal, Jean-Claude Guillemot, Marie Gaille

médecine/sciences 31:91-111 (2015)10.1051/medsci/201531s1006

2014

2014
La chémobiologie : stratégies transfrontalières

Laurence Lafanechère, Eric Maréchal, Jean-Claude Guillemot

médecine/sciences 30:2 (2014)10.1051/medsci/20143012001

2014
Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes

Mickaël Bouvet, Adrien Lugari, Clara C. Posthuma, Jessika C. Zevenhoven, Stéphanie Bernard, Stéphane Betzi, Isabelle Imbert, Bruno Canard, Jean-Claude Guillemot, Patrick Lécine, Susanne Pfefferle, Christian Drosten, Eric J. Snijder, Etienne Decroly, Xavier Morelli

Journal of Biological Chemistry 289:25783-25796 (2014)10.1074/jbc.M114.577353

2013

2013
Plasma Peptide Biomarker Discovery for Amyotrophic Lateral Sclerosis by MALDI –TOF Mass Spectrometry Profiling

Laurence Conraux, Catherine Pech, Halim Guerraoui, Denis Loyaux, Pascual Ferrara, Jean-Claude Guillemot, Vincent Meininger, Pierre-François Pradat, François Salachas, Gaelle Bruneteau, Nadine Le Forestier, Lucette Lacomblez

PLoS ONE 8:e79733 (2013)10.1371/journal.pone.0079733

2012

2012
Antiviral chlorinated daphnane diterpenoid orthoesters from the bark and wood of Trigonostemon cherrieri.

Pierre-Marie Allard, Pieter Leyssen, Marie-Thérèse Martin, Mélanie Bourjot, Vincent Dumontet, Cécilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Cyril Poullain, Françoise Guéritte, Marc Litaudon

Phytochemistry 84:160-8 (2012)10.1016/j.phytochem.2012.07.023

2012
Chemical constituents of Anacolosa pervilleana and their antiviral activities.

Mélanie Bourjot, Pieter Leyssen, Cécilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Philippe Rasoanaivo, Françoise Guéritte, Marc Litaudon

Fitoterapia 83:1076-1080 (2012)10.1016/j.fitote.2012.05.004

2012
Flacourtosides A-F, Phenolic Glycosides Isolated from Flacourtia ramontchi.

Mélanie Bourjot, Pieter Leyssen, Cécilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Philippe Rasoanaivo, Françoise Guéritte, Marc Litaudon

Journal of Natural Products 75:752-8 (2012)10.1021/np300059n

2011

2011
Alkylated flavanones from the bark of Cryptocarya chartacea as dengue virus NS5 polymerase inhibitors.

Pierre-Marie Allard, Elise Tran Huu Dau, Cécilia Eydoux, Jean-Claude Guillemot, Vincent Dumontet, Cyril Poullain, Bruno Canard, Françoise Guéritte, Marc Litaudon

Journal of Natural Products 74:2446-53 (2011)10.1021/np200715v

2011
PHYTOCHIK: Biodiversity As A Source of Selective Inhibitors of CHIKV Replication

Pieter Leyssen, Marc Litaudon, Jean-Claude Guillemot, Philippe Rasoanaivo, Jacqueline Smadja, Ameenah Gurib-Fakim, Bruno Canard, Françoise Guéritte

Antiviral Research 90:A36-A37 (2011)10.1016/j.antiviral.2011.03.048

2009

2009
Identification of allosteric inhibitors blocking the hepatitis C virus polymerase NS5B in the RNA synthesis initiation step.

Stéphane Betzi, Cécilia Eydoux, Cécile Bussetta, Marilyne Blemont, Pieter Leyssen, Claire Debarnot, Mohamed Ben-Rahou, Jacques Haiech, Marcel Hibert, Françoise Gueritte, David S. Grierson, Jean-Louis Romette, Jean-Claude Guillemot, Johan Neyts, Karine Alvarez, Xavier Morelli, Hélène Dutartre, Bruno Canard

Antiviral Research 84:48-59 (2009)10.1016/j.antiviral.2009.06.009

2006

2006
Comparative mechanistic studies of de novo RNA synthesis by flavivirus RNA-dependent RNA polymerases

Barbara Selisko, Hélène Dutartre, Jean-Claude Guillemot, Claire Debarnot, Delphine Benarroch, Alexander Khromykh, Philippe Desprès, Marie-Pierre Egloff, Bruno Canard

Virology 351:145-158 (2006)10.1016/j.virol.2006.03.026

2005

2005
A Relaxed Discrimination of 2′- O -Methyl-GTP Relative to GTP between de Novo and Elongative RNA Synthesis by the Hepatitis C RNA-dependent RNA Polymerase NS5B

Hélène Dutartre, Joëlle Boretto, Jean Claude Guillemot, Bruno Canard

Journal of Biological Chemistry 280:6359-6368 (2005)10.1074/jbc.M410191200

2002

2002
Identification of two tamoxifen target proteins by photolabeling with 4-(2-morpholinoethoxy)benzophenone.

Fabienne Mésange, Mohamed Sebbar, Joël Capdevielle, Jean-Claude Guillemot, Pascual Ferrara, Francis Bayard, Marc E. Poirot, Jean-Charles Faye

Bioconjug Chem 13:766-72 (2002)

1998

1998
Tyrosine phosphorylation of the Wiskott-Aldrich Syndrome protein by Lyn and Btk is regulated by CDC42

Rodolphe r. Guinamard, Pontus Aspenström, Michel Fougereau, Philippe Chavrier, Jean-Claude Guillemot

FEBS Letters 434:431-436 (1998)10.1016/s0014-5793(98)01016-3

1998
Microsomal epoxide hydrolase of rat liver is a subunit of the anti-oestrogen-binding site.

Fabienne Mésange, Mohamed Sebbar, Blandine Kedjouar, Joël Capdevielle, Jean-Claude Guillemot, Pascual Ferrara, Francis Bayard, Frédéric Delarue, Jean-Charles Faye, Marc E. Poirot

Biochemical Journal 334 ( Pt 1):107-12 (1998)