CNRS - AIX MARSEILLE UNIV: UMR7257


Our laboratory


The Architecture et Fonction des Macromolécules Biologiques (AFMB) laboratory, overseen by the CNRS and AMU, is a center of structural biology localized on the Luminy campus in the South of Marseilles, France, and in partnership with INRA under contract. The aim of our researches is to depict the architecture of proteins or macromolecular assemblies at the molecular level to understand the biological mechanisms which they govern. The description of interactions between macromolecules or a macromolecule and a ligand is also crucial to manipulate these complex molecules. Finally, to understand the molecular diversity of members of some protein families, we also analyze the big data issued from large-scale genome sequencing centers.


Seminars
  • Monday 24th of September at 11am: Andrei Lupas, Max Planck Institute for Developmental Biology, Dept. Protein Evolution, Tübingen, Allemagne "The Path from Ancestral Polypeptide Disorder to Autonomous Protein Folding (and back)"
    All seminars
  • Tuesday 25th of September at 11am: Stefan Kolek, Douglas Instruments, UK "New strategies to improve productivity - rMMS microseeding for crystallization and DLS for cryoEM"

    Abstract (...)

    Abstract Random Microseed Matrix-Screening (rMMS), where seed crystals are added automatically to random crystallization screens, is a significant recent breakthrough in protein crystallization [1]. During the ten years since the method was published, understanding of the theoretical advantages of the method has increased [2 - 4], and several practical variations on the basic method have emerged. Important variations that will be discussed include combining seeds from several hits [5], the best methods of selecting hits to optimize [2], and cross-seeding targets with crystals of homologous proteins [6]. Single-particle cryoEM clearly has great potential to determine the structures of macromolecules when crystallographic approaches are not available. The throughput of cryoEM is, however, low, with only one or a few samples being analysed per day. This creates a need for a pre-screening approach to investigate the behaviour of macromolecules in solution. Douglas Instruments has worked closely with Xtal Concepts to develop a screening approach were e.g. 96 wells can be analysed by DLS using only a few microlitres of sample [8, 9]. This concept will be outlined briefly, with examples.
    All seminars
News
  • This August, the work of Baptiste Martin, Etienne Decroly and colleagues is highlighted by the INSB dept of the CNRS
    voir
  • The work of Adeline Goulet, Christian Cambillau and colleagues is also spotlighted this August, by the INSB dept of the CNRS
    voir
Events
  • European Researchers’ Night 2018, Sept 28: at Marseille, Dock des Suds, the AFMB lab will be there!
    voir



AFMB - UMR7257 CNRS - Aix-Marseille Univ. - Case 932
163 Avenue de Luminy 13288 Marseille CEDEX 09 FRANCE


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