This PBSIM service offers the latest technologies for production of soluble eukaryotic proteins in mammalian (HEK293, CHO, COS-7) or insect cells (S2, Sf9), which are able to process most of the post-translational modifications (disulfide bridges, N- and O-glycosylation, phosphorylation…) critical for protein structure or function.
The feasibility study associates the specification needs of the applicant (protein amounts, tags for purification and detection…) and the expertise of the facility staff.
The selected protocol is assayed, validated and then amplified for a quantitative production assay. The produced proteins are purified and characterized by the applicants.
Academia and industries