Denis Ptchelkine
Research engineer
Publications
2025
A conserved motif in Henipavirus P/V/W proteins drives the fibrillation of the W protein from Hendra virus
Frank Gondelaud, Christophe Bignon, Denis Ptchelkine, Frédéric Carrière, Sonia Longhi
Protein Science 34 (2025)10.1002/pro.70085
Unraveling the molecular grammar and the structural transitions underlying the fibrillation of a viral fibrillogenic domain
Frank Gondelaud, Julien Leval, Lisha Arora, Anuja Walimbe, Christophe Bignon, Denis Ptchelkine, Stefania Brocca, Samrat Mukhopadyay, Sonia Longhi
Protein Science 34:e70068 (2025)10.1002/pro.70068
2024
Alphavirus nsP3 organizes into tubular scaffolds essential for infection and the cytoplasmic granule architecture
Vasiliya Kril, Michael Hons, Celine Amadori, Claire Zimberger, Laurine Couture, Yara Bouery, Julien Burlaud-Gaillard, Andrei Karpov, Denis Ptchelkine, Alexandra Thienel, Beate Kümmerer, Ambroise Desfosses, Rhian Jones, Philippe Roingeard, Laurent Meertens, Ali Amara, Juan Reguera
Nature Communications 15:8106 (2024)10.1038/s41467-024-51952-z
Dissecting Henipavirus W proteins conformational and fibrillation properties: contribution of their N‐ and C‐terminal constituent domains
Giulia Pesce, Frank Gondelaud, Denis Ptchelkine, Christophe Bignon, Patrick Fourquet, Sonia Longhi
FEBS Journal Online ahead of print (2024)10.1111/febs.17239
Functional redundancy revealed by the deletion of the mimivirus GMC-oxidoreductase genes
Jean-Marie Alempic, Hugo Bisio, Alejandro Villalta, Sébastien Santini, Audrey Lartigue, Alain Schmitt, Claire Bugnot, Anna Notaro, Lucid Belmudes, Annie Adrait, Olivier Poirot, Denis Ptchelkine, Cristina de Castro, Yohann Couté, Chantal Abergel
microLife 5 (2024)10.1093/femsml/uqae006
2023
Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies
Juliet F Nilsson, Hakima Baroudi, Frank Gondelaud, Giulia Pesce, Christophe Bignon, Denis Ptchelkine, Joseph Chamieh, Hervé Cottet, Andrey V Kajava, Sonia Longhi
International Journal of Molecular Sciences 24:399 (2023)10.3390/ijms24010399
2022
Experimental Evidence of Intrinsic Disorder and Amyloid Formation by the Henipavirus W Proteins
Giulia Pesce, Frank Gondelaud, Denis Ptchelkine, Juliet F Nilsson, Christophe Bignon, Jérémy Cartalas, Patrick Fourquet, Sonia Longhi
International Journal of Molecular Sciences 23:923 (2022)10.3390/ijms23020923
Functional benefit of structural disorder for the replication of measles, Nipah and Hendra viruses
Frank Gondelaud, Giulia Pesce, Juliet F. Nilsson, Christophe Bignon, Denis Ptchelkine, Denis Gerlier, Cyrille Mathieu, Sonia Longhi
Essays in Biochemistry 66:915-934 (2022)10.1042/EBC20220045
2021
The HelQ human DNA repair helicase utilizes a PWI-like domain for DNA loading through interaction with RPA, triggering DNA unwinding by the HelQ helicase core
Tabitha Jenkins, Sarah J Northall, Denis Ptchelkine, Rebecca Lever, Andrew Cubbon, Hannah Betts, Vincenzo Taresco, Christopher D O Cooper, Peter J Mchugh, Panos Soultanas, Edward L Bolt
NAR Cancer 3 (2021)10.1093/narcan/zcaa043
2020
2′-Alkynyl spin-labelling is a minimally perturbing tool for DNA structural analysis
Jack S Hardwick, Marius M Haugland, Afaf H El-Sagheer, Denis Ptchelkine, Frank R Beierlein, Andrew N Lane, Tom Brown, Janet E Lovett, Edward A Anderson
Nucleic Acids Research 48:2830-2840 (2020)10.1093/nar/gkaa086
2019
Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors
Adam M Thomas, Sanja Brolih, Joanna F Mcgouran, Afaf H El-Sagheer, Denis Ptchelkine, Morgan Jones, Neil Q Mcdonald, Peter J Mchugh, Tom Brown
Chemical Communications 55:11671-11674 (2019)10.1039/c9cc05476f
2017
X-ray crystallography and NMR show that 5-formylcytosine does not change the global structure of DNA
Jack S Hardwick, Denis Ptchelkine, Afaf H El-Sagheer, Ian Tear, Daniel Singleton, Simon E V Phillips, Andrew N Lane, Tom Brown
Nature Structural and Molecular Biology 24:544-552 (2017)10.1038/nsmb.3411
Intracellular immunization against HIV infection with an intracellular antibody that mimics HIV integrase binding to the cellular LEDGF protein
Leyuan Bao, Clare Hannon, Abimael Cruz-Mignoni, Denis Ptchelkine, Mei-Yi Sun, Ami Miller, Wilawan Bunjobpol, Camilo E Quevedo, Mariliza Derveni, Jennifer Chambers, Alison Simmons, Simon E V Phillips, Terence H Rabbitts
Scientific Reports 7 (2017)10.1038/s41598-017-16742-2
Unique architecture of thermophilic archaeal virus APBV1 and its genome packaging
Denis Ptchelkine, Ashley Gillum, Tomohiro Mochizuki, Soizick Lucas-Staat, Ying Liu, Mart Krupovic, Simon Phillips, David Prangishvili, Juha Huiskonen
Nature Communications 8:1436 (2017)10.1038/s41467-017-01668-0
RPA activates the XPF-ERCC1 endonuclease to initiate processing of DNA interstrand crosslinks
Ummi B Abdullah, Joanna F Mcgouran, Sanja Brolih, Denis Ptchelkine, Afaf H El‐sagheer, Tom Brown, Peter J Mchugh
EMBO Journal 36:2047-2060 (2017)10.15252/embj.201796664
2012
Structural Framework for Covalent Inhibition of Clostridium botulinum Neurotoxin A by Targeting Cys165.
Enrico A Stura, Laura Le Roux, Karine Guitot, Sandra Garcia, Sarah Bregant, Fabrice Beau, Laura Vera, Guillaume Collet, Denis Ptchelkine, Huseyin Bakirci, Vincent Dive
Journal of Biological Chemistry 287:33607-14 (2012)10.1074/jbc.M112.396697
2011
Mass Spectrometry Reveals Stable Modules in holo and apo RNA Polymerases I and III
Laura A Lane, Carlos Fernández-Tornero, Min Zhou, Nina Morgner, Denis Ptchelkine, Ulrich Steuerwald, Argyris Politis, Doris Lindner, Jelena Gvozdenovic, Anne-Claude Gavin, Christoph W Müller, Carol V Robinson
Structure 19:90-100 (2011)10.1016/j.str.2010.11.009
2010
Structural Basis of TBX5–DNA Recognition: The T-Box Domain in Its DNA-Bound and -Unbound Form
Christian Stirnimann, Denis Ptchelkine, Clemens Grimm, Christoph Müller
Journal of Molecular Biology 400:71-81 (2010)10.1016/j.jmb.2010.04.052
2008
Structural basis for LEAFY floral switch function and similarity with helix-turn-helix proteins.
Cécile Hamès, Denis Ptchelkine, Clemens Grimm, Emmanuel Thevenon, Edwige Moyroud, Francine Gérard, Jean-Louis Martiel, Reyes Benlloch, François Parcy, Christoph W Müller
EMBO Journal 27:2628-37 (2008)10.1038/emboj.2008.184
