Our research projects address the molecular architectures and functional mechanisms of carbohydrate-active enzymes (CAZymes), and of enzymes, receptors/channels, and cell adhesion molecules with a neurobiological interest.

The CAZymes govern the hydrolysis, conversion or recognition of sugar moieties from glycoconjuguates widely found in animals, fungi, plants and bacteria. These conjugates are involved in key biological processes such as development, quality control of protein folding, host-pathogen recognition, immune response, and they can be linked to diseases such as neurodegenerative metabolic diseases. In collaboration with the “Glycogenomics” team we pay particular attention to those glycoside hydrolases that are of interest for biomedical and biotechnological applications.

We also address the function, recognition properties and structure of receptors, enzymes and adhesion molecules involved in central and peripheral neurotransmission, with particular interest for their structure-function relationships, specificity of partner and ligand recognition, conformational dynamics and functional regulation. In particular we have accumulated expertise in the study of the enzyme acetylcholinesterase and of the extracellular ligand-binding domain of the nicotinic acetylcholine receptor, whose malfunctioning has been correlated with neurodegenerative diseases, and to cell-adhesion molecules structurally related to the cholinesterases or to other families of proteins and associated with developmental neuronal deficiencies.

Our research also focuses on the structural and functional study of membrane sensor proteins involved in biofilm formation in the pathogen Pseudomonas aeruginosa, or on enzymes involved in lipid metabolism in the pathogen Mycobacterium tuberculosis.

Our methodological approaches involve recombinant protein expression in prokaryotic and eukaryotic systems and complementary biochemical, biophysical, functional and structural (X-ray crystallography and electron microscopy) characterization means.

Domaines de recherche
  • glycobiology
  • enzyme mechanism
  • neurobiology
  • molecular interactions

Members

Past members

  • Caroline Peneff, PhD student, 1999-2001
  • Jordan Meynard, Master-2 BBSG student, 2003
  • David Veesler, Master-1 BBSG student, 2004
  • Dorothée Bauguil, 2nd year ESIL (now Polytech Marseille) student, 2004
  • Abraham Saliba, Master-2 BBSG student, 2004
  • Julien Offant, PhD student, 2003-2005
  • Isabelle Dezoize, 2nd year ESIL (now Polytech Marseille) student, 2006
  • Alexandra Lipski, Master 2 BBSG and PhD student, 2006-2008
  • David Dal Molin, 2nd year ESIL (now Polytech Marseille) student, 2008
  • Mahfoud Balki, Master-2 BBSG student, 2009
  • Emma Bossion, 2nd yrs BTS student Lycée La Forbine, 2009
  • Marie-Cécile Pélissier, Master-2 BBSG and PhD student, 2006-2009 (ATER 2009)
  • Goetz Parsiegla, Maître de Conférences, 2001-2010
  • Allan Lamontanara, 2nd year ESIL (now Polytech Marseille) student, 2010
  • Daniel Gonzales, Master-1 BBSG student, 2010
  • Nahla Dhen, Master-2 BBSG student, 2011
  • Julie Prats, 2nd year ESIL (now Polytech Marseille) student, 2012
  • Guillaume Ponchel, Master-2 BBSG and PhD student, 2008-2012
  • Isabelle Mosnier, engineer assistant, 2011-2012
  • Claire Zimberger, BTS student Lycée Marie Curie, 1rst and 2nd years, 2011 and 2012
  • Thibaud Freyd, Master-2 BBSG student, 2012
  • Camille Ribeyre, Master-1 BBSG student, 2013
  • Nicolas Lenfant, postdoc/visitor INRA Montpellier, 2011-2013
  • Audrey Blanchard, study engineer, 2011-2014
  • Supanee Potisopon, postdoc/research engineer, 2014
  • Florian Mourey, 2nd year AgroParisTech student, 2014
  • Stanley Germany, Master-2 BBSG student, 2014
  • Romain La Rocca, L3 student, 2015
  • Aurélien Assice-Vivoni, study engineer, 2015
  • Julie Baptiste, study engineer, 2015-2016
  • Camille Garcia, L3 student, 2016
  • Sandy Bezenger, Master-2 BBSG student, 2016
  • Farida Nezlioui, Master-2 BBSG student, 2016
  • Ahmad Ali-Ahmad, Master-2 BBSG and PhD student, 2011-2016
  • Maria Mate, research engineer CNRS, 2013-2017
  • Marie-Julie Dejardin, study engineer, 2016-2017
  • Adeline Goulet, research engineer, then postdoc FRM, and then CNRS researcher, 2013-2017
  • Yoann Crétinon, Master-1 BBSG student, 2017
  • Marina Gomes, Master-1 and Master-2 BBSG student, 2016-2017
  • Quoc-Phong Nguyen, Master-2 student, 2017
  • Melvin Boumedour, 1rst and 2nd yrs BTS student Lycée La Forbine, 2017
  • Lamia Mebarki, PhD student, then study/research engineer, 2014-2017
  • Amani Ghanem, Master-2 BBSG student, 2018
  • Viet-Anh Hoang, Master-2 student, Vietnam, 2018
  • Firas Fadel, post-doctoral fellow, 2014-2018
  • Elodie Caillol, L3 student, Univ Grenoble Alpes, 2019
  • Sarah Coulibaly Martineau, Master-2 Biologie Structurale, Génomique (BSG) 2019
  • Djouhaina Benmehidi, 2nd yr Sup’Biotech Paris, 2019
  • Antonin Gardais, 4th yr Polytech Marseille, 2019
  • Nicola Curci, visiting PhD student Univ Naples ‘Federico II’, 2019
  • Jean-Baptiste Boitel, study engineer, 2018-2019
  • Semeli Platsaki, post-doctoral fellow, 2016-2019
  • Christelle Saadé, Master-2 student, Lebanon, 2020
  • Anh-Phu Nguyen, Master-2 student, Vietnam, 2020
  • Phuoc-Dung Nguyen, Master-2 student, Vietnam, 2020
  • Violla Bassim, Master-2 student Lebanon then PhD student AMU, 2017-2020
  • Marine Papin, study engineer, 2020-2021
  • Cynthia Lohberger, Master-1 Biologie Structurale, Génomique (BSG), 2021
  • Lynda Guezout, Master-1 Biologie Moléculaire et Cellulaire Grenoble Alpes Univ, 2021
  • Nafisatou Drame, Master-2 Biologie Structurale, Génomique (BSG), 2021
  • Fran Miočić-Stošić, Master-2 Univ Zagreb, Croatia, 2021
  • Claire Bedez, post-doctoral fellow, 2019-2021

Publications

2022
Insights into the atypical autokinase activity of the Pseudomonas aeruginosa GacS histidine kinase and its interaction with RetS

Firas Fadel, Violla Bassim, Vanessa Francis, Steven Porter, Thomas Botzanowski, Pierre Legrand, Maria Maté Perez, Yves Bourne, Sarah Cianférani, Florence Vincent

Structure (2022)10.1016/j.str.2022.06.002

2022
Patient-derived antibodies reveal the subcellular distribution and heterogeneous interactome of LGI1

Jorge Ramirez-Franco, Kévin Debreux, Johanna Extremet, Yves Maulet, Maya Belghazi, Claude Villard, Marion Sangiardi, Fahamoe Youssouf, Lara El Far, Christian Lévêque, Claire Debarnot, Pascale Marchot, Sofija Paneva, Dominique Debanne, Michael Russier, Michael Seagar, Sarosh Irani, Oussama El Far

Brain - A Journal of Neurology (2022)10.1093/brain/awac218

2022
Molecular basis for substrate recognition and septum cleavage by AtlA, the major N-acetylglucosaminidase of Enterococcus faecalis

Véronique Roig-Zamboni, Sarah Barelier, Robert Dixon, Nicola Galley, Amani Ghanem, Quoc Phong Nguyen, Héloize Cahuzac, Bartłomiej Salamaga, Peter Davis, Yves Bourne, Stéphane Mesnage, Florence Vincent

Journal of Biological Chemistry 298:101915 (2022)10.1016/j.jbc.2022.101915

Show complete publications list

Team publications

2022
Insights into the atypical autokinase activity of the Pseudomonas aeruginosa GacS histidine kinase and its interaction with RetS

Firas Fadel, Violla Bassim, Vanessa Francis, Steven Porter, Thomas Botzanowski, Pierre Legrand, Maria Maté Perez, Yves Bourne, Sarah Cianférani, Florence Vincent

Structure (2022)10.1016/j.str.2022.06.002

2022
Patient-derived antibodies reveal the subcellular distribution and heterogeneous interactome of LGI1

Jorge Ramirez-Franco, Kévin Debreux, Johanna Extremet, Yves Maulet, Maya Belghazi, Claude Villard, Marion Sangiardi, Fahamoe Youssouf, Lara El Far, Christian Lévêque, Claire Debarnot, Pascale Marchot, Sofija Paneva, Dominique Debanne, Michael Russier, Michael Seagar, Sarosh Irani, Oussama El Far

Brain - A Journal of Neurology (2022)10.1093/brain/awac218

2022
Molecular basis for substrate recognition and septum cleavage by AtlA, the major N-acetylglucosaminidase of Enterococcus faecalis

Véronique Roig-Zamboni, Sarah Barelier, Robert Dixon, Nicola Galley, Amani Ghanem, Quoc Phong Nguyen, Héloize Cahuzac, Bartłomiej Salamaga, Peter Davis, Yves Bourne, Stéphane Mesnage, Florence Vincent

Journal of Biological Chemistry 298:101915 (2022)10.1016/j.jbc.2022.101915

2021
Comparative mapping of selected structural determinants on the extracellular domains of cholinesterase-like cell-adhesion molecules.

Davide Comoletti, Laura Trobiani, Arnaud Chatonnet, yves Bourne, P. Marchot

Neuropharmacology 184:108381 (2021)10.1016/j.neuropharm.2020.108381

2021
Carnitine is a pharmacological allosteric chaperone of the human lysosomal α -glucosidase

Roberta Iacono, Nadia Minopoli, Maria Carmina Ferrara, Antonietta Tarallo, Carla Damiano, Caterina Porto, Sandra Strollo, Véronique Roig-Zamboni, Gianfranco Peluso, Gerlind Sulzenbacher, Beatrice Cobucci-Ponzano, Giancarlo Parenti, Marco Moracci

Journal of Enzyme Inhibition and Medicinal Chemistry 36:2068-2079 (2021)10.1080/14756366.2021.1975694

2021
First insights into the structural features of Ebola virus methyltransferase activities

Coralie Valle, Baptiste Martin, François Ferron, Véronique Roig-Zamboni, Aline Desmyter, Françoise Debart, Jean Jacques Vasseur, Bruno Canard, Bruno Coutard, Etienne Decroly

Nucleic Acids Research 49:1737-1748 (2021)10.1093/nar/gkaa1276

2020
The neuroligins and the synaptic pathway in Autism Spectrum Disorder.

Laura Trobiani, Maria Meringolo, Tamara Diamanti, yves Bourne, P. Marchot, Giuseppina Martella, Luciana Dini, Antonio Pisani, Antonella de Jaco, Paola Bonsi

Neuroscience and Biobehavioral Reviews 119:37-51 (2020)10.1016/j.neubiorev.2020.09.017

2020
Biochemical characterization of a glycosyltransferase Gtf3 from Mycobacterium smegmatis: a case study of improved protein solubilization

Mahfoud Bakli, Loukmane Karim, Nassima Mokhtari-Soulimane, Hafida Merzouk, Florence Vincent

3 Biotech 10 (2020)10.1007/s13205-020-02431-x

2020
The Ig-like domain of Punctin/MADD-4 is the primary determinant for interaction with the ectodomain of neuroligin NLG-1

Semeli Platsaki, Xin Zhou, Bérangère Pinan-Lucarré, Vincent Delauzun, Haijun Tu, Pascal Mansuelle, Patrick Fourquet, yves Bourne, Jean-Louis Bessereau, P. Marchot

Journal of Biological Chemistry 16267-16279. (2020)10.1074/jbc.RA120.014591

2020
Snapshots of ADp-ribose bound to Getah virus macro domain reveal an intriguing choreography

Ana Sofia Ferreira-Ramos, Gerlind Sulzenbacher, Bruno Canard, Bruno Coutard

Scientific Reports 10 (2020)10.1038/s41598-020-70870-w

2019
Phase transition and amyloid formation by a viral protein as an additional molecular mechanism of virus-induced cell toxicity

Edoardo Salladini, Claire Debarnot, Vincent Delauzun, Maria Grazia Murrali, Priscila Sutto-Ortiz, Silvia Spinelli, Roberta Pierattelli, Christophe Bignon, Sonia Longhi

(2019)

2019
Substrate binding mode and catalytic mechanism of human heparan sulfate d-glucuronyl C5 epimerase

Claire Debarnot, yoan Monneau, Veronique Roig-Zamboni, Vincent Delauzun, Christine Le Narvor, Emeline Richard, Jérôme Hénault, Adeline Goulet, Firas Fadel, Romain R Vivès, Bernard Priem, David Bonnaffé, Hugues Lortat-Jacob, yves Bourne

Proceedings of the National Academy of Sciences of the United States of America 116:6760-6765 (2019)10.1073/pnas.1818333116

2019
Overview of the Structure–Function Relationships of Mannose-Specific Lectins from Plants, Algae and Fungi

Annick Barre, yves Bourne, Els J. M. van Damme, Pierre Rougé

International Journal of Molecular Sciences 20:254 (2019)10.3390/ijms20020254

2019
An evolutionary perspective on the first disulfide bond in members of the cholinesterase-carboxylesterase (COesterase) family: Possible outcomes for cholinesterase expression in prokaryotes

Arnaud Chatonnet, Xavier Brazzolotto, Thierry Hotelier, Nicolas Lenfant, P. Marchot, yves Bourne

Chemico-Biological Interactions 308:179-184 (2019)10.1016/j.cbi.2019.05.016

2018
Genetic and enzymatic characterization of 3-O-sulfotransferase SNPs associated with Plasmodium falciparum parasitaemia

Ngoc Thy Nguyen, Romain R Vivès, Magali Iché-Torres, Vincent Delauzun, Els Saesen, Veronique Roig-Zamboni, Hugues Lortat-Jacob, Pascal Rihet, yves Bourne

Glycobiology 28:534-541 (2018)10.1093/glycob/cwy038

2018
Lytic xylan oxidases from wood-decay fungi unlock biomass degradation

Marie Couturier, Simon Ladeveze, Gerlind Sulzenbacher, Luisa Ciano, Mathieu Fanuel, Céline Moreau, Ana Villares, Bernard Cathala, Florence Chaspoul, Kristian Frandsen, Aurore Labourel, Isabelle Gimbert Herpoël-Gimbert, Sacha Grisel, Mireille Haon, Nicolas Lenfant, Hélène Rogniaux, David Ropartz, Gideon Davies, Marie-Noelle Rosso, Paul Walton, Bernard Henrissat, Jean-Guy Berrin

Nature Chemical Biology 14:306-310 (2018)10.1038/nchembio.2558

2018
Glycosylate and move! The glycosyltransferase Maf is involved in bacterial flagella formation

Gerlind Sulzenbacher, Veronique Roig-Zamboni, Régine Lebrun, yann Guérardel, Dorothée Murat, Pascal Mansuelle, Nao yamakawa, Xin-Xin Qian, Renaud Vincentelli, yves Bourne, Long-Fei Wu, François Alberto

Environmental Microbiology 20:228-240 (2018)10.1111/1462-2920.13975

2018
Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes

Wade Abbott, Orly Alber, Ed Bayer, Jean-Guy Berrin, Alisdair Boraston, Harry Brumer, Ryszard Brzezinski, Anthony Clarke, Beatrice Cobucci-Ponzano, Darrell Cockburn, Pedro Coutinho, Mirjam Czjzek, Bareket Dassa, Gideon John Davies, Vincent Eijsink, Jens Eklof, Alfons Felice, Elizabeth Ficko-Blean, Geoff Pincher, Thierry Fontaine, Zui Fujimoto, Kiyotaka Fujita, Shinya Fushinobu, Harry Gilbert, Tracey Gloster, Ethan Goddard-Borger, Ian Greig, Jan-Hendrik Hehemann, Glyn Hemsworth, Bernard Henrissat, Masafumi Hidaka, Ramon Hurtado-Guerrero, Kiyohiko Igarashi, Takuya Ishida, Stefan Janecek, Seino Jongkees, Nathalie Juge, Satoshi Kaneko, Takane Katayama, Motomitsu Kitaoka, Naotake Konno, Daniel Kracher, Anna Kulminskaya, Alicia Lammerts van Bueren, Sine Larsen, Junho Lee, Markus Linder, Leila Loleggio, Roland Ludwig, Ana Luis, Mirko Maksimainen, Brian Mark, Richard Mclean, Gurvan Michel, Cédric Montanier, Marco Moracci, Haruhide Mori, Hiroyuki Nakai, Wim Nerinckx, Takayuki Ohnuma, Richard Pickersgill, Kathleen Piens, Tirso Pons, Etienne Rebuffet, Peter Reilly, Magali Remaud Simeon, Brian Rempel, Kyle Robinson, David Rose, Juha Rouvinen, Wataru Saburi, yuichi Sakamoto, Mats Sandgren, Fathima Shaikh, yuval Shoham, Franz St John, Jerry Stahlberg, Michael Suits, Gerlind Sulzenbacher, Tomomi Sumida, Ryuichiro Suzuki, Birte Svensson, Toki Taira, Ed Taylor, Takashi Tonozuka, Breeanna Urbanowicz, Gustav Vaaje-Kolstad, Wim van den Ende, Annabelle Varrot, Maxime Versluys, Florence Vincent, David Vocadlo, Warren Wakarchuk, Tom Wennekes, Rohan Williams, Spencer Williams, David Wilson, Stephen Withers, Katsuro yaoi, Vivian yip, Ran Zhang

Glycobiology 28:3-8 (2018)10.1093/glycob/cwx089

2017
Structural and functional insights into the periplasmic detector domain of the GacS histidine kinase controlling biofilm formation in Pseudomonas aeruginosa

Ahmad Ali-Ahmad, Firas Fadel, Corinne Sebban-Kreuzer, Moly Ba, Gauthier Dangla Pélissier, Olivier Bornet, Françoise Guerlesquin, yves Bourne, Christophe Bordi, Florence Vincent

Scientific Reports 7:11262 (2017)10.1038/s41598-017-11361-3

2017
Structure of human lysosomal acid α-glucosidase-a guide for the treatment of Pompe disease

Véronique Roig-Zamboni, Beatrice Cobucci-Ponzano, Roberta Iacono, Maria Carmina Ferrara, Stanley Germany, yves Bourne, Giancarlo Parenti, Marco Moracci, Gerlind Sulzenbacher

Nature Communications (2017)10.1038/s41467-017-01263-3

2017
Diversity in the binding interactions of marine phycotoxins to AChBP, the soluble nAChR surrogate

P. Marchot, Gerlind Sulzenbacher, Zoran Radic, Rómulo Aráoz, Emmanuelle Reynaud, Evelyne Benoit, Armen Zakarian, Denis Servent, Jordi Molgó, Palmer Taylor, yves Bourne

142:196 (2017)10.1111/jnc.13925

2017
Cyclic imine toxins from dinoflagellates: a growing family of potent antagonists of the nicotinic acetylcholine receptors

Jordi Molgó, P. Marchot, Rómulo Aráoz, Evelyne Benoit, Bogdan I Iorga, Armen Zakarian, Palmer Taylor, yves Bourne, Denis Servent

Journal of Neurochemistry 142:41-51 (2017)10.1111/jnc.13995

2017
NMR assignments of the GacS histidine-kinase periplasmic detection domain from Pseudomonas aeruginosa PAO1

Ahmad Ali-Ahmad, Olivier Bornet, Firas Fadel, yves Bourne, Florence Vincent, Christophe Bordi, Françoise Guerlesquin, Corinne Sebban-Kreuzer

Biomolecular NMR Assignments 11:25-28 (2017)10.1007/s12104-016-9714-7

2017
Structural insights into a family 39 glycoside hydrolase from the gut symbiont Bacteroides cellulosilyticus WH2

Ahmad Ali-Ahmad, Marie-Line Garron, Véronique Zamboni, Nicolas Lenfant, Didier Nurizzo, Bernard Henrissat, Jean-Guy Berrin, yves Bourne, Florence Vincent

Journal of Structural Biology 197:227-235 (2017)10.1016/j.jsb.2016.11.004

2017
Structure of human lysosomal acid α-glucosidase–a guide for the treatment of Pompe disease

Veronique Roig-Zamboni, Beatrice Cobucci-Ponzano, Roberta Iacono, Maria Carmina Ferrara, Stanley Germany, yves Bourne, Giancarlo Parenti, Marco Moracci, Gerlind Sulzenbacher

Nature Communications 8:1111 (2017)10.1038/s41467-017-01263-3

2016
Relationships of human alpha/beta hydrolase fold proteins and other organophosphate-interacting proteins

Nicolas Lenfant, yves Bourne, P. Marchot, Arnaud Chatonnet

Chemico-Biological Interactions 259:343-351 (2016)10.1016/j.cbi.2016.04.027

2016
ESTHER database: update on the alpha/beta hydrolase fold superfamily of proteins

Arnaud Chatonnet, P. Marchot, yves Bourne, Nicolas Lenfant, Thierry Hotelier

142:226 p. (2016)

2016
Evolution of animal cholinesterases

Nicolas Lenfant, Murray E. Selkirk, Thierry Hotelier, yves Bourne, P. Marchot, Arnaud Chatonnet

142:226 p. (2016)

2016
Steric and Dynamic Parameters Influencing In Situ Cycloadditions to Form Triazole Inhibitors with Crystalline Acetylcholinesterase

yves Bourne, K. Barry Sharpless, Palmer Taylor, P. Marchot

Journal of the American Chemical Society 138:1611-1621 (2016)10.1021/jacs.5b11384

2016
The Quaternary Structure of a Glycoside Hydrolase Dictates Specificity toward β-Glucans

Mickael Lafond, Gerlind Sulzenbacher, Thibaud Freyd, Bernard Henrissat, Jean-Guy Berrin, Marie-Line Garron

Journal of Biological Chemistry 291:7183-7194 (2016)10.1074/jbc.M115.695999

2015
2015
Nanoscale Architecture of the Axon Initial Segment Reveals an Organized and Robust Scaffold

Christophe Leterrier, Jean J. Potier, Ghislaine Caillol, Claire Debarnot, Fanny Rueda boroni, Bénédicte Dargent

Cell Reports 13:2781-2793 (2015)10.1016/j.celrep.2015.11.051

2015
CK2-regulated schwannomin-interacting protein IQCJ-SCHIP-1 association with AnkG contributes to the maintenance of the axon initial segment

Marie-Jeanne Papandréou, Hélène Vacher, Marie-Pierre Fache, Esther Klingler, Fanny Rueda-Boroni, Géraldine Ferracci, Claire Debarnot, Christelle Pipéroglou, Gontzal Garcia del Caño, Laurence Goutebroze, Bénédicte Dargent

Journal of Neurochemistry 134:527-537 (2015)10.1111/jnc.13158

2015
Marine Macrocyclic Imines, Pinnatoxins A and G: Structural Determinants and Functional Properties to Distinguish Neuronal α7 from Muscle α12βγδ nAChRs.

yves Bourne, Gerlind Sulzenbacher, Zoran Radić, Rómulo Aráoz, Morgane Reynaud, Evelyne Benoit, Armen Zakarian, Denis Servent, Jordi Molgó, Palmer Taylor, P. Marchot

Structure (London, England : 1993) 23:1106-15 (2015)10.1016/j.str.2015.04.009

2015
Structural basis for carbohydrate binding properties of a plant chitinase-like agglutinin with conserved catalytic machinery

Gerlind Sulzenbacher, Veronique Roig-Zamboni, Willy J. Peumans, Bernard Henrissat, Els J. M. Van Damme, yves Bourne

Journal of Structural Biology 190:115-121 (2015)10.1016/j.jsb.2015.01.013

2015
2014
Functional characterization of a vacuolar invertase from Solanum lycopersicum: Post-translational regulation by N-glycosylation and a proteinaceous inhibitor

Alexandra S Tauzin, Gerlind Sulzenbacher, Mickael Lafond, Véronique Desseaux, Ida Barbara Reca, Josette Perrier, Daniela Bellincampi, Patrick Fourquet, Christian Lévêque, Thierry Giardina

Biochimie (2014)10.1016/j.biochi.2013.12.013

2014
The neuroligins and their neurexin ligands: from structure to function at the synapse

yves Bourne, P. Marchot

Journal of Molecular Neuroscience 53:387-396 (2014)10.1007/s12031-014-0234-6

2014
Structural and biochemical characterization of the β-N-acetylglucosaminidase from Thermotoga maritima: toward rationalization of mechanistic knowledge in the GH73 family

Alexandra Lipski, Mireille Hervé, Vincent Lombard, Didier Nurizzo, Dominique Mengin-Lecreulx, yves Bourne, Florence Vincent

Glycobiology 25:319-330 (2014)10.1093/glycob/cwu113

2014
Tracking the origin and divergence of cholinesterases and neuroligins: the evolution of synaptic proteins

Nicolas Lenfant, Thierry Hotelier, yves Bourne, P. Marchot, Arnaud Chatonnet

Journal of Molecular Neuroscience 53:362-369 (2014)10.1007/s12031-013-0194-2

2014
Structural and Functional Characterization of the Clostridium perfringens N-Acetylmannosamine-6-phosphate 2-Epimerase Essential for the Sialic Acid Salvage Pathway

Marie-Cécile Pélissier, Corinne Sebban-Kreuzer, Françoise Guerlesquin, James A Brannigan, yves Bourne, Florence Vincent

Journal of Biological Chemistry 289:35215-35224 (2014)10.1074/jbc.m114.604272

2013
Molecular Characterization of Monoclonal Antibodies that Inhibit Acetylcholinesterase by Targeting the Peripheral Site and Backdoor Region

yves Bourne, Ludovic Renault, Sosthène Essono, Grégoire Mondielli, Patricia Lamourette, Didier Boquet, Jacques Grassi, P. Marchot

PLoS ONE 8:e77226 (2013)10.1371/journal.pone.0077226

2013
Tracking origin and divergence of Cholinesterases and Neuroligins

Nicolas Lenfant, Thierry Hotelier, yves Bourne, P. Marchot, Arnaud Chatonnet

1 p. (2013)

2013
ESTHER, the database of the α/β-hydrolase fold superfamily of proteins: tools to explore diversity of functions

Nicolas Lenfant, Thierry Hotelier, Eric Velluet, yves Bourne, P. Marchot, Arnaud Chatonnet

Nucleic Acids Research 41:423-429 (2013)10.1093/nar/gks1154

2013
Proteins with an alpha/beta hydrolase fold: Relationships between subfamilies in an ever-growing superfamily.

Nicolas Lenfant, Thierry Hotelier, yves Bourne, P. Marchot, Arnaud Chatonnet

Chemico-Biological Interactions 203:266-268 (2013)10.1016/j.cbi.2012.09.003

2012
The Structure of Human DNase I Bound to Magnesium and Phosphate Ions Points to a Catalytic Mechanism Common to Members of the DNase I-like Superfamily

Goetz Parsiegla, Christophe Noguere, Lydia Santell, Robert Lazarus, yves Bourne

Biochemistry 51:10250-10258 (2012)10.1021/bi300873f

2012
Exploitation of β -glycosyl azides for the preparation of α -glycosynthases

Beatrice Cobucci-Ponzano, Carmela Zorzetti, Andrea Strazzulli, Emiliano Bedini, Maria Michela Corsaro, Gerlind Sulzenbacher, Mos È Rossi, Marco Moracci

Biocatalysis and Biotransformation (2012)10.3109/10242422.2012.679814

2012
Structural Insights into Antibody Sequestering and Neutralizing of Na+ Channel α-Type Modulator from Old World Scorpion Venom.

Igor P Fabrichny, Grégoire Mondielli, Sandrine Conrod, Marie-France Martin-Eauclaire, yves Bourne, P. Marchot

Journal of Biological Chemistry 287:14136-48 (2012)10.1074/jbc.M111.315382

2012
Fragment-guided design of subnanomolar beta-lactamase inhibitors active in vivo

Oliv Eidam, Chiara Romagnoli, Guillaume Dalmasso, Sarah Barelier, Emilia Caselli, Richard Bonnet, Brian K. Shoichet, Fabio Prati

Proceedings of the National Academy of Sciences of the United States of America 109:17448-17453 (2012)10.1073/pnas.1208337109

2011
α-Galactosidase/Sucrose Kinase (AgaSK), a Novel Bifunctional Enzyme from the Human Microbiome Coupling Galactosidase and Kinase Activities

Laëtitia Bruel, Gerlind Sulzenbacher, Marine Cervera Tison, Ange Pujol, Cendrine Nicoletti, Josette Perrier, Anne Galinier, David Ropartz, Michel Fons, Frederique Pompeo, Thierry Giardina

Journal of Biological Chemistry 286:40814-40823 (2011)10.1074/jbc.M111.286039

2011
Ligand specificity, privileged substructures and protein druggability from fragment-based screening

Sarah Barelier, Isabelle Krimm

Current Opinion in Chemical Biology 15:469-474 (2011)10.1016/j.cbpa.2011.02.020

2011
Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex.

Claire Debarnot, Isabelle Imbert, Francois Ferron, Laure Gluais, Isabelle Varlet, Nicolas Papageorgiou, Mickaël Bouvet, Julien Lescar, Etienne Decroly, Bruno Canard

Acta crystallographica Section F : Structural biology communications [2014-...] 67:404-8 (2011)10.1107/S1744309111002867

2010
Conformational remodeling of femtomolar inhibitor-acetylcholinesterase complexes in the crystalline state.

yves Bourne, Zoran Radić, Palmer Taylor, P. Marchot

Journal of the American Chemical Society 132:18292-300 (2010)10.1021/ja106820e

2010
Crystal structures of a family 8 polysaccharide lyase reveal open and highly occluded substrate-binding cleft conformations

Zainab H Elmabrouk, Florence Vincent, Meng Zhang, Nicola L Smith, Johan P Turkenburg, Simon J Charnock, Gary W Black, Edward J Taylor

Proteins - Structure, Function and Bioinformatics 79:965-974 (2010)10.1002/prot.22938

2010
Structural insights into the exquisite selectivity of neurexin/neuroligin synaptic interactions.

Philippe Leone, Davide Comoletti, Géraldine Ferracci, Sandrine Conrod, Simon U Garcia, Palmer Taylor, yves Bourne, P. Marchot

EMBO Journal 29:2461-71 (2010)10.1038/emboj.2010.123

2010
Structure of a polyisoprenoid binding domain from Saccharophagus degradans implicated in plant cell wall breakdown

Florence Vincent, David Dal Molin, Ronald Weiner, yves Bourne, Bernard Henrissat

FEBS Letters 584:1577-1584 (2010)10.1016/j.febslet.2010.03.015

2010
Structural determinants in phycotoxins and AChBP conferring high affinity binding and nicotinic AChR antagonism

yves Bourne, Zoran Radić, Rómulo Aráoz, Todd Talley, Evelyne Benoit, Denis Servent, Palmer Taylor, Jordi Molgó, Pascale Marchot

Proceedings of the National Academy of Sciences of the United States of America 107:6076-6081 (2010)10.1073/pnas.0912372107

2010
Structural determinants in phycotoxins and AChBP conferring high affinity binding and nicotinic AChR antagonism.

yves Bourne, Zoran Radic, Rómulo Aráoz, Todd T Talley, Evelyne Benoit, Denis Servent, Palmer Taylor, Jordi Molgó, P. Marchot

Proceedings of the National Academy of Sciences of the United States of America epub ahead of print (2010)10.1073/pnas.0912372107

2010
Structure-function relationships of the alpha/beta-hydrolase fold domain of neuroligin: A comparison with acetylcholinesterase.

Philippe Leone, Davide Comoletti, Palmer Taylor, yves Bourne, P. Marchot

Chem Biol Interact epub ahead of print (2010)10.1016/j.cbi.2010.01.030

2010
Crystal Structure of the GalNAc/Gal-Specific Agglutinin from the Phytopathogenic Ascomycete Sclerotinia sclerotiorum Reveals Novel Adaptation of a β-Trefoil Domain

Gerlind Sulzenbacher, Véronique Roig-Zamboni, Willy J Peumans, Pierre Rougé, Els J M van Damme, yves Bourne

Journal of Molecular Biology 400:715-723 (2010)10.1016/j.jmb.2010.05.038

2010
A novel α-N-acetylgalactosaminidase family with an NAD+-dependent catalytic mechanism suitable for enzymatic removal of blood group A antigens

Gerlind Sulzenbacher, Qiyong Peter Liu, Eric P Bennett, Steven B Levery, yves Bourne, Guillaume Ponchel, Henrik Clausen, Bernard Henrissat

Biocatalysis and Biotransformation 28:22-32 (2010)10.3109/10242420903424259

2009
Interaction between the SifA Virulence Factor and Its Host Target SKIP Is Essential for Salmonella Pathogenesis

Lautaro Diacovich, Audrey Dumont, Daniel Lafitte, Elodie Soprano, Aude-Agnès Guilhon, Christophe Bignon, Jean-Pierre Gorvel, yves Bourne, Stéphane Méresse

Journal of Biological Chemistry 284:33151-33160 (2009)10.1074/jbc.m109.034975

2009
b-Glycosyl Azides as Substrates for a-Glycosynthases: Preparation of Efficient a-L-Fucosynthases

Beatrice Cobucci-Ponzano, Fiorella Conte, Emiliano Bedini, Maria Michela Corsaro, Michelangelo Parrilli, Gerlind Sulzenbacher, Alexandra Lipski, Dal Fabrizio, Laura Lepore, Mosè Rossi, Marco Moracci

Chemistry and Biology (2009)10.1016/j.chembiol.2009.09.013

2009
Identification of allosteric inhibitors blocking the hepatitis C virus polymerase NS5B in the RNA synthesis initiation step.

Stéphane Betzi, Cécilia Eydoux, Cécile Bussetta, Marilyne Blemont, Pieter Leyssen, Claire Debarnot, Mohamed Ben-Rahou, Jacques Haiech, Marcel Hibert, Françoise Gueritte, David S. Grierson, Jean-Louis Romette, Jean-Claude Guillemot, Johan Neyts, Karine Alvarez, Xavier Morelli, Hélène Dutartre, Bruno Canard

Antiviral Research 84:48-59 (2009)10.1016/j.antiviral.2009.06.009

2009
Interaction between the sifa virulence factor and its host target skip is essential for salmonella pathogenesis.

Lautaro Diacovich, Audrey Dumont, Daniel Lafitte, Elodie Soprano, Aude-Agnes Guilhon, Christophe Bignon, Jean-Pierre Gorvel, yves Bourne, Stephane Meresse

Journal of Biological Chemistry epub ahead of print (2009)10.1074/jbc.M109.034975

2009
Structural determinants for interaction of partial agonists with acetylcholine binding protein and neuronal α7 nicotinic acetylcholine receptor

Ryan Hibbs, Gerlind Sulzenbacher, Jianxin Shi, Todd Talley, Sandrine Conrod, William Kem, Palmer Taylor, P. Marchot, yves Bourne

EMBO Journal 28:3040-3051 (2009)10.1038/emboj.2009.227

2009
Distinct oligomeric forms of the Pseudomonas aeruginosa RetS sensor domain modulate accessibility to the ligand binding site

Florence Vincent, Adam Round, Aline Reynaud, Christophe Bordi, Alain Filloux, yves Bourne

Environmental Microbiology 12:1775-1786 (2009)10.1111/j.1462-2920.2010.02264.x

2009
Tumor Protein 53–Induced Nuclear Protein 1 Is a Major Mediator of p53 Antioxidant Function

Carla E Cano, Julien Gommeaux, Sylvia Pietri, Marcel Culcasi, Stéphane Garcia, Mylène Seux, Sarah Barelier, Sophie Vasseur, Rose P Spoto, Marie-Josèphe Pébusque, Nelson J Dusetti, Juan Lucio Iovanna, Alice Carrier

Cancer Research 69:219-226 (2009)10.1158/0008-5472.CAN-08-2320

2008
Glycosyltransferases, glycoside hydrolases: surprise, surprise!

Bernard Henrissat, Gerlind Sulzenbacher, yves Bourne

Current Opinion in Structural Biology (2008)10.1016/j.sbi.2008.09.003

2008
Identification of a GH110 Subfamily of ␣1,3-Galactosidases: NOVEL ENZYMES FOR REMOVAL OF THE ␣3GAL XENOTRANSPLANTATION ANTIGEN

Qiyong P Liu, Huaiping yuan, Eric P Bennett, Steven B Levery, Edward Nudelman, Jean Spence, Greg Pietz, Kristen Saunders, Thayer White, Martin L Olsson, Bernard Henrissat, Gerlind Sulzenbacher, Henrik Clausen

Journal of Biological Chemistry 283:8545-8554 (2008)10.1074/jbc.M709020200

2008
Utilisation des " protéines liant l'acétylcholine " (AChBP) en toxinologie structurale : nouveaux exemples

yves Bourne, Z. Radic, Tt Talley, S. Conrod, P. Taylor, Jordi Molgó, P. Marchot

27-8 (2008)

2007
Structural Analysis of the Synaptic Protein Neuroligin and Its β-Neurexin Complex: Determinants for Folding and Cell Adhesion

Igor Fabrichny, Philippe Leone, Gerlind Sulzenbacher, Davide Comoletti, Meghan Miller, Palmer Taylor, yves Bourne, P. Marchot

Neuron 56:979-991 (2007)10.1016/j.neuron.2007.11.013

2007
Des glycosidases bactériennes pour du sang universel

Gerlind Sulzenbacher, yves Bourne, Bernard Henrissat

médecine/sciences (2007)10.1051/medsci/20072389703

2007
Crystal Structure of the RNA Polymerase Domain of the West Nile Virus Non-structural Protein 5

Hélène Malet, Marie-Pierre Egloff, Barbara Selisko, Rebecca E Butcher, Peter J Wright, Michael Roberts, Arnaud Gruez, Gerlind Sulzenbacher, Clemens Vonrhein, Gérard Bricogne, Jason M Mackenzie, Alexander A Khromykh, Andrew D Davidson, Bruno Canard

Journal of Biological Chemistry (2007)10.1074/jbc.M607273200

2007
Bacterial glycosidases for the production of universal red blood cells

Qiyong P Liu, Gerlind Sulzenbacher, Huaiping yuan, Eric P Bennett, Greg Pietz, Kristen Saunders, Jean Spence, Edward Nudelman, Steven B Levery, Thayer White, John M Neveu, William S Lane, yves Bourne, Martin L Olsson, Bernard Henrissat, Henrik Clausen

Nature Biotechnology (2007)10.1038/nbt1298

2007
Gene overexpression and biochemical characterization of the biotechnologically relevant chlorogenic acid hydrolase from Aspergillus niger

Isabelle Benoit, Michèle Asther, yves Bourne, David Navarro, Stéphane Canaan, Laurence Lesage-Meessen, Marga Herweijer, Pedro M Coutinho, Marcel M. Asther, Eric Record

Applied and Environmental Microbiology 73:5624-5632 (2007)10.1128/AEM.00374-07

2006
Substrate and product trafficking through the active center gorge of acetylcholinesterase analyzed by crystallography and equilibrium binding.

yves Bourne, Zoran Radic, Gerlind Sulzenbacher, Esther Kim, Palmer Taylor, P. Marchot

Journal of Biological Chemistry 281:29256-67 (2006)10.1074/jbc.M603018200

2006
Comparative mechanistic studies of de novo RNA synthesis by flavivirus RNA-dependent RNA polymerases

Barbara Selisko, Hélène Dutartre, Jean-Claude Guillemot, Claire Debarnot, Delphine Benarroch, Alexander Khromykh, Philippe Desprès, Marie-Pierre Egloff, Bruno Canard

Virology 351:145-158 (2006)10.1016/j.virol.2006.03.026

2006
Functional characterization of the glycosyltransferase domain of penicillin-binding protein 1a from Thermotoga maritima

Julien Offant, François Michoux, Annabelle Dermiaux, Jacques Biton, yves Bourne

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1764:1036-1042 (2006)10.1016/j.bbapap.2006.03.012

2006
LppX is a lipoprotein required for the translocation of phthiocerol dimycocerosates to the surface of Mycobacterium tuberculosis

Gerlind Sulzenbacher, Stéphane Canaan, yann Bordat, Olivier Neyrolles, Gustavo Stadthagen, Véronique Roig-Zamboni, Jean Rauzier, Damien Maurin, Françoise Laval, Mamadou Daffé, Christian Cambillau, Brigitte Gicquel, yves Bourne, Mary Jackson

EMBO Journal (2006)10.1038/sj.emboj.7601048

2006
Structure and Activity of Two Metal Ion-dependent Acetylxylan Esterases Involved in Plant Cell Wall Degradation Reveals a Close Similarity to Peptidoglycan Deacetylases

Edward J. Taylor, Tracey M. Gloster, Johan P. Turkenburg, Florence Vincent, A. Marek Brzozowski, Claude Dupont, François Shareck, Maria S.J. Centeno, José A.M. Prates, Vladimír Puchart, Luís M.A. Ferreira, Carlos M.G.A. Fontes, Peter Biely, Gideon J. Davies

Journal of Biological Chemistry 281:10968-10975 (2006)10.1074/jbc.M513066200

2006
Structural characterization of agonist and antagonist-bound acetylcholine-binding protein from Aplysia californica.

Scott B Hansen, Gerlind Sulzenbacher, Tom Huxford, P. Marchot, yves Bourne, Palmer Taylor

Journal of Molecular Neuroscience 30:101-2 (2006)

2006
Respective importance of protein folding and glycosylation in the thermal stability of recombinant feruloyl esterase A

Isabelle Benoit, Michèle Asther, Gerlind Sulzenbacher, Eric Record, Laurence Marmuse, Goetz Parsiegla, Isabelle Gimbert, Marcel M. Asther, Christophe Bignon

FEBS Letters 580:5815-5821 (2006)10.1016/j.febslet.2006.09.039

2006
Structural comparison of three crystalline complexes of a peptidic toxin with a synaptic acetylcholine recognition protein.

yves Bourne, Scott B Hansen, Gerlind Sulzenbacher, Todd T Talley, Tom Huxford, Palmer Taylor, P. Marchot

Journal of Molecular Neuroscience 30:103-4 (2006)

2005
Structural insights into AChE inhibition by monoclonal antibodies

Ludovic Renault, Sosthène Essono, Marianick Juin, Didier Boquet, Jacques Grassi, yves Bourne, P. Marchot

Chemico-Biological Interactions 157-158:397-400 (2005)10.1016/j.cbi.2005.10.073

2005
A. niger protein “EstA”, perhaps a new electrotactin, defines a new class of fungal esterases within the α/β hydrolase fold superfamily

yves Bourne, Alinda Hasper, Henri Chahinian, Ludovic Renault, Marianick Juin, Leo de Graaff, P. Marchot

Chemico-Biological Interactions 157-158:395-396 (2005)10.1016/j.cbi.2005.10.072

2005
Structure and Kinetics of a Monomeric Glucosamine 6-Phosphate Deaminase

Florence Vincent, Gideon J Davies, James A Brannigan

Journal of Biological Chemistry 280:19649-19655 (2005)10.1074/jbc.m502131200

2005
Crystal structure of the conserved hypothetical protein Rv1155 from Mycobacterium tuberculosis

Stéphane Canaan, Gerlind Sulzenbacher, Véronique Roig-Zamboni, Loréna Scappuccini-Calvo, Frédéric Frassinetti, Damien Maurin, Christian Cambillau, yves Bourne, Christian Griesinger

FEBS Letters (2005)10.1016/j.febslet.2004.11.069

2005
Structures of Aplysia AChBP complexes with nicotinic agonists and antagonists reveal distinctive binding interfaces and conformations

Scott B Hansen, Gerlind Sulzenbacher, Tom Huxford, P. Marchot, Palmer Taylor, yves Bourne

EMBO Journal 24:3635-3646 (2005)10.1038/sj.emboj.7600828

2005
Structural insights into conformational flexibility at the peripheral site and within the active center gorge of AChE, Chem Biol Interact. 157-158, 159-65.

yves Bourne, Z. Radic, Kolb H. C., Sharpless K. B., P. Taylor, P. Marchot

Chemico-Biological Interactions 157-158:159-65. (2005)

2005
Crystal structure of a Cbtx-AChBP complex reveals essential interactions between snake alpha-neurotoxins and nicotinic receptors,

yves Bourne, T. T. Talley, Hansen S. B., P. Taylor, P. Marchot

EMBO Reports 24:1512-1522 (2005)

2004
The Use of Forced Protein Evolution to Investigate and Improve Stability of Family 10 Xylanases

Simon Andrews, Edward Taylor, Gavin Pell, Florence Vincent, Valérie M.-A. Ducros, Gideon Davies, Jeremy Lakey, Harry Gilbert

Journal of Biological Chemistry 279:54369-54379 (2004)10.1074/jbc.M409044200

2004
Common Inhibition of Both β-Glucosidases and β-Mannosidases by Isofagomine Lactam Reflects Different Conformational Itineraries for Pyranoside Hydrolysis

Florence Vincent, Tracey Gloster, James Macdonald, Carl Morland, Robert Stick, Fernando Dias, José Prates, Carlos Fontes, Harry Gilbert, Gideon Davies

ChemBioChem 5:1596-1599 (2004)10.1002/cbic.200400169

2004
Structure of Escherichia coli YhdH, a putative quinone oxidoreductase

Gerlind Sulzenbacher, Véronique Roig-Zamboni, Fabienne Pagot, Sacha Grisel, Aurelia Salomoni, Christel Valencia, Valérie Campanacci, Renaud Vincentelli, Mariella Tegoni, Hans Eklund, Christian Cambillau

Acta crystallographica Section D : Structural biology [1993-...] (2004)10.1107/S0907444904020220

2004
Crystal structures of bovine odorant-binding protein in complex with odorant molecules

Florence Vincent, Roberto Ramoni, Silvia Spinelli, Stefano Grolli, Mariella Tegoni, Christian Cambillau

European Journal of Biochemistry 271:3832-3842 (2004)10.1111/j.1432-1033.2004.04315.x

2004
Insights into the Molecular Determinants of Substrate Specificity in Glycoside Hydrolase Family 5 Revealed by the Crystal Structure and Kinetics of Cellvibrio mixtus Mannosidase 5A

Fernando M V Dias, Florence Vincent, Gavin Pell, José a M Prates, Maria S J Centeno, Louise E Tailford, Luís M A Ferreira, Carlos M G A Fontes, Gideon J Davies, Harry J Gilbert

Journal of Biological Chemistry 279:25517-25526 (2004)10.1074/jbc.m401647200

2004
The Three-dimensional Structure of Invertase (β-Fructosidase) from Thermotoga maritima Reveals a Bimodular Arrangement and an Evolutionary Relationship between Retaining and Inverting Glycosidases

Francois Alberto, Christophe Bignon, Gerlind Sulzenbacher, Bernard Henrissat, Mirjam Czjzek

Journal of Biological Chemistry 279:18903-18910 (2004)10.1074/jbc.M313911200

2004
Aspergillus niger Protein EstA Defines a New Class of Fungal Esterases within the α/β Hydrolase Fold Superfamily of Proteins

yves Bourne, Alinda A Hasper, Henri Chahinian, Marianick Juin, Leo H de Graaff, P. Marchot

Structure 12:677-687 (2004)10.1016/j.str.2004.03.005

2004
Crystal Structure of Thermotoga maritima ␣-L-Fucosidase INSIGHTS INTO THE CATALYTIC MECHANISM AND THE MOLECULAR BASIS FOR FUCOSIDOSIS

Gerlind Sulzenbacher, Christophe Bignon, Takeshi Nishimura, Chris A Tarling, Stephen G Withers, Bernard Henrissat, yves Bourne

Journal of Biological Chemistry (2004)10.1074/jbc.M313783200

2004
Freeze-frame inhibitor captures acetylcholinesterase in a unique conformation

y. Bourne, H. Kolb, Z. Radic, K. Sharpless, P. Taylor, P. Marchot

Proceedings of the National Academy of Sciences of the United States of America 101:1449-1454 (2004)10.1073/pnas.0308206100

2004
Crystal Structure of E. coli Alcohol Dehydrogenase YqhD: Evidence of a Covalently Modified NADP Coenzyme

Gerlind Sulzenbacher, Karine Alvarez, Robert H H van den Heuvel, Cees Versluis, Silvia Spinelli, Valé Rie Campanacci, Christel Valencia, Christian Cambillau, Hans Eklund, Mariella Tegoni

Journal of Molecular Biology 342:489-502 (2004)10.1016/j.jmb.2004.07.034

2004
Crystal Structure of E. coli YddE Protein Reveals a Striking Homology With Diaminopimelate Epimerase

Alice Grassick, Gerlind Sulzenbacher, Véronique Roig-Zamboni, Valérie Campanacci, Christian Cambillau, yves Bourne

Proteins - Structure, Function and Bioinformatics 55:764-767 (2004)10.1002/prot.20025

2003
Identification of the Catalytic Nucleophile of the Family 29 α-L-Fucosidase from Thermotoga maritima through Trapping of a Covalent Glycosyl-Enzyme Intermediate and Mutagenesis

Chris A Tarling, Shouming He, Gerlind Sulzenbacher, Christophe Bignon, yves Bourne, Bernard Henrissat, Stephen G Withers

Journal of Biological Chemistry (2003)10.1074/jbc.M306610200

2003
The Three-dimensional Structure of the N-Acetylglucosamine-6-phosphate Deacetylase, NagA, from Bacillus subtilis

Florence Vincent, David yates, Elspeth Garman, Gideon J Davies, James A Brannigan

Journal of Biological Chemistry 279:2809-2816 (2003)10.1074/jbc.m310165200

2003
Multifunctional Xylooligosaccharide/Cephalosporin C Deacetylase Revealed by the Hexameric Structure of the Bacillus subtilis Enzyme at 1.9Å Resolution

Florence Vincent, Simon Charnock, Koen H.G. Verschueren, Johan Turkenburg, David Scott, Wendy Offen, Shirley Roberts, Gavin Pell, Harry Gilbert, Gideon Davies, James Brannigan

Journal of Molecular Biology 330:593-606 (2003)10.1016/s0022-2836(03)00632-6

2003
Medium-Scale Structural Genomics: Strategies for Protein Expression and Crystallization

Renaud Vincentelli, Christophe Bignon, Arnaud Gruez, Stéphane Canaan, Gerlind Sulzenbacher, Mariella Tegoni, Rie Campanacci, Christian Cambillau

Accounts of Chemical Research (2003)10.1021/ar010130s

2003
Structural insights into ligand interactions at the acetylcholinesterase peripheral anionic site

yves Bourne, Palmer Taylor, Zoran Radic, P. Marchot

EMBO Journal 22:1-12 (2003)10.1093/emboj/cdg005

2002
Boar salivary lipocalin

Silvia Spinelli, Florence Vincent, Paolo Pelosi, Mariella Tegoni, Christian Cambillau

European Journal of Biochemistry 269:2449-2456 (2002)10.1046/j.1432-1033.2002.02901.x

2002
Mechanism of Acetylcholinesterase Inhibition by Fasciculin: A 5-ns Molecular Dynamics Simulation

Kaihsu Tai, Tongye Shen, Richard Henchman, yves Bourne, P. Marchot, J. Andrew Mccammon

Journal of the American Chemical Society 124:6153-6161 (2002)10.1021/ja017310h

2002
Control of domain swapping in bovine odorant-binding protein

Roberto Ramoni, Florence Vincent, Alison E Ashcroft, Paolo Accornero, Stefano Grolli, Christel Valencia, Mariella Tegoni, Christian Cambillau

Biochemical Journal (2002)

2002
A medium-throughput crystallization approach

Gerlind Sulzenbacher, Arnaud Gruez, Véronique Roig-Zamboni, Silvia Spinelli, Christel Valencia, Fabienne Pagot, Renaud Vincentelli, Christophe Bignon, Aurelia Salomoni, Sacha Grisel, Damien Maurin, Céline Huyghe, Kent Johansson, Alice Grassick, Alain Roussel, yves Bourne, Sophie Perrier, Linda Miallau, Phillippe Cantau, Eric Blanc, Michel Genevois, Alain Grossi, André Zenatti, Valérie Campanacci, Christian Cambillau

Acta crystallographica Section D : Structural biology [1993-...] 58:2109-2115 (2002)10.1107/s0907444902013938

2001
Crystal Structure of Streptococcus pneumoniae N -Acetylglucosamine-1-phosphate Uridyltransferase Bound to Acetyl-coenzyme A Reveals a Novel Active Site Architecture

Gerlind Sulzenbacher, Laurent Gal, Caroline Peneff, Florence Fassy, yves Bourne

Journal of Biological Chemistry 276:11844-11851 (2001)10.1074/jbc.M011225200

2001
Crystal structure of aphrodisin, a sex pheromone from female hamster11Edited by R Huber

Florence Vincent, Dietrich Löbel, Kieron Brown, Silvia Spinelli, Phillip Grote, Heinz Breer, Christian Cambillau, Mariella Tegoni

Journal of Molecular Biology 305:459-469 (2001)10.1006/jmbi.2000.4241

2001
The insect attractant 1-Octen-3-o1 is the natural ligand of bovine odorant-binding protein

Roberto Ramoni, Florence Vincent, Stefano Grolli, Virna Conti, Christian Malosse, Francois Boyer, Patricia Nagnan-Le Meillour, Silvia Spinelli, Christian Cambillau, Mariella Tegoni

Journal of Biological Chemistry 276:7150-7155 (2001)10.1074/jbc.M010368200

2000
Immunocytochemical Localization and Crystal Structure of Human Frequenin (Neuronal Calcium Sensor 1)

yves Bourne, Jens Dannenberg, Verena Pollmann, P. Marchot, Olaf Pongs

Journal of Biological Chemistry 276:11949-11955 (2000)10.1074/jbc.m009373200

2000
Mammalian odorant binding proteins

Mariella Tegoni, Paolo Pelosi, Florence Vincent, Silvia Spinelli, Valérie Campanacci, Stefano Grolli, Roberto Ramoni, Christian Cambillau

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 1482:229-240 (2000)10.1016/s0167-4838(00)00167-9

2000
Complexes of porcine odorant binding protein with odorant molecules belonging to different chemical classes

Florence Vincent, Silvia Spinelli, Roberto Ramoni, Stefano Grolli, Paolo Pelosi, Christian Cambillau, Mariella Tegoni

Journal of Molecular Biology 300:127-139 (2000)10.1006/jmbi.2000.3820

1999
RNA recognition by transcriptional antiterminators of the BglG/SacY family: functional and structural comparison of the CAT domain from SacY and LicT

Nathalie Declerck, Florence Vincent, François Hoh, Stéphane Aymerich, Herman van Tilbeurgh

Journal of Molecular Biology 294:389-402 (1999)10.1006/jmbi.1999.3256

1999
Conformational Flexibility of the Acetylcholinesterase Tetramer Suggested by X-ray Crystallography*

yves Bourne, Jacques Grassi, Pierre E Bougis, P. Marchot

Journal of Biological Chemistry 274:30370-30376 (1999)

1999
Catalysis and specificity in enzymatic glycoside hydrolysis: a 2,5B conformation for the glycosyl-enzyme intermediate revealed by the structure of the Bacillus agaradhaerens family 11 xylanase

Elisabetta Sabini, Gerlind Sulzenbacher, Miroslava Dauter, Zbigniew Dauter, Per Linå Jørgensen, Martin Schülein, Claude Dupont, Gideon Davies, Keith Wilson

Chemistry and Biology 6:483-492 (1999)10.1016/s1074-5521(99)80066-0

1999
Crystal Structure of Mouse Acetylcholinesterase

yves Bourne, Palmer Taylor, Pierre E Bougis, P. Marchot

Journal of Biological Chemistry 274:2963-2970 (1999)10.1074/jbc.274.5.2963

1999
The Crystal Structure of a 2-Fluorocellotriosyl Complex of the Streptomyces lividans Endoglucanase CelB2 at 1.2 Å Resolution † , ‡

Gerlind Sulzenbacher, Lloyd F Mackenzie, Keith S Wilson, Stephen G Withers, Claude Dupont, Gideon J Davies

Biochemistry 38:4826-4833 (1999)10.1021/bi982648i

1998
Inhibition of mouse acetylcholinesterase by fasciculin: Crystal structure of the complex and mutagenesis of fasciculin

P. Marchot, yves Bourne, Claudine Prowse, Pierre Bougis, Palmer Taylor

Toxicon 36:1613-1622 (1998)10.1016/s0041-0101(98)00154-8

1998
Barley alpha-amylase bound to its endogenous protein inhibitor BASI: crystal structure of the complex at 1.9 A resolution.

F. Vallee, A. Kadziola, yves Bourne, M. Juy, Kw Rodenburg, B. Svensson, R. Haser

Structure 6:649-659 (1998)

1998
Crystal structure of mouse acetylcholinesterase

yves Bourne, P Taylor, J R Kanter, Pierre E Bougis, P. Marchot

315-322 (1998)

1998
Crystal structure of the family 7 endoglucanase I (Cel7B) from Humicola insolens at 2.2 Å resolution and identification of the catalytic nucleophile by trapping of the covalent glycosyl-enzyme intermediate

Lloyd F Mackenzie, Gerlind Sulzenbacher, Christina Divne, T. Alwyn Jones, Helle Wöldike, Martin Schülein, Stephen G Withers, Gideon J Davies

Biochemical Journal 335:409-416 (1998)10.1042/bj3350409

1997
The Streptomyces lividans Family 12 Endoglucanase: Construction of the Catalytic Core, Expression, and X-ray Structure at 1.75 Å Resolution

Gerlind Sulzenbacher, François Shareck, Rolf Morosoli, Claude Dupont, Gideon J Davies

Biochemistry 36:16032-16039 (1997)10.1021/bi972407v

1996
Soluble monomeric acetylcholinesterase from mouse: Expression, purification, and crystallization in complex with fasciculin

P. Marchot, Raimond B.G. Ravelli, Mia Raves, yves Bourne, Daniel Vellom, Joan Kanter, Shelley Camp, Joel Sussman, Palmer Taylor

Protein Science 5:672-679 (1996)10.1002/pro.5560050411

1994
Structures of a legume lectin complexed with the human lactotransferrin N2 fragment, and with an isolated biantennary glycopeptide: role of the fucose moiety.

yves Bourne, J. Mazurier, Delphine Legrand, Pierre Rougé, J. Montreuil, G. Spik, C. Cambillau

Structure / Struct Fold Des; Structure (Camb ) 2:209-19 (1994)

1994
Primary and three-dimensional structure of lactotransferrin (lactoferrin) glycans.

G. Spik, B. Coddeville, J. Mazurier, yves Bourne, C. Cambillaut, J. Montreuil

Advances in Experimental Medicine and Biology 357:21-32 (1994)

1993
Structural versatility of peptides from C α, α -disubstituted glycines: Crystal-state conformational analysis of homopeptides from C α -methyl, C α -benzylglycine [(αMe)Phe] n

Giovanni Valle, Monica Pantano, Fernando Formaggio, Marco Crisma, Claudio Toniolo, Gilles Précigoux, Gerlind Sulzenbacher, Wilhelmus Boesten, Quirinus Broxterman, Hans E Schoemaker, Johan Kamphuis

Biopolymers 33:1617-1625 (1993)10.1002/bip.360331011

1993
Oligosaccharide conformation in protein/carbohydrate complexes

A. Imberty, yves Bourne, C. Cambillau, Pascal Rougé, Sarah Perez

(1993)

1992
Crystallization and preliminary X-ray diffraction study of Lathyrus ochrus isolectin II complexed to the human lactotransferrin N2 fragment.

yves Bourne, M. P. Nésa, Pierre Rougé, J. Mazurier, Delphine Legrand, G. Spik, J. Montreuil, C. Cambillau

Journal of Molecular Biology 227:938-41 (1992)

1992
Conformational analysis of oligosaccharides complexed with lectins

A. Imberty, yves Bourne, C. Cambillau, Pascal Rougé, Sarah Perez

1 p. (1992)

1989
Crystallization and preliminary X-ray study of horse pancreatic lipase

Dominique Lombardo, Catherine Chapus, yves Bourne, Christian Cambillau

Journal of Molecular Biology 205:259-261 (1989)10.1016/0022-2836(89)90380-x

1988
Crystallization and preliminary X-ray studies of two isolectins from the seeds of Lathyrus ochrus

yves Bourne, Pierre Rougé, Christian Cambillau

Journal of Molecular Biology 202:685-687 (1988)10.1016/0022-2836(88)90299-9

Network

National
  • Jean-Louis Bessereau, Bérangère Pinan-Lucarre 
    • INMG, CNRS, INSERM, U. Lyon
  • Arnaud Chatonnet
    • DMeM, INRAE, U. Montpellier
  • Sylvie Diochot, Gérard Lambeau
    • IPMC, CNRS, U. Côte d’Azur, Valbonne
  • Oussama El Far, Dominique Debanne
    • UNIS, INSERM, AMU, Marseille
  • Jordi Molgó, Denis Servent
    • SIMoS, CNRS, CEA-Saclay, Gif-sur-Yvette
  • Aziz Moqrich, Francis Castet, Flavio Maina
    • IBDM, CNRS, AMU, Marseille
  • Tafalgie Therapeutics SAS
    • Marseille
  • Sarah Cianférani, Oscar Hernandez
    • IPHC, U. Strasbourg
  • Pascal Villa
    • PCBIS, U. Strasbourg
  • Jean-Guy Berrin
    • BBF, INRAE, AMU, Marseille
  • Sandrine Py
    • DCM-SERCO, U. Grenoble- Alpes
  • Frédérique Pompeo
    • LCB, CNRS, Marseille
  • François Alberto
    • LCB, Marseille
  • Stéphane Canaan, Jean-François Cavalier
    • LISM, CNRS, Marseille
  • Hugues Lortat-Jacob, Romain Vivès, Rebekka Wild
    • IBS, Grenoble
  • Kevin Baranger
    • LIENSs, La Rochelle
  • Jérôme Nigou, Julien Marcoux
    • IPBS, Toulouse
International
  • Davide Comoletti 
    • Victoria U. of Wellington, NZ
  • Antonella De Jaco
    • Sapienza U. of Rome, IT
  • Akos Nemecz
    • Institute of Biology, UMK, Toruń, PL
  • Palmer Taylor
    • SSPPS, UCSD, La Jolla (CA), USA
  • Armen Zakarian
    • UCSB, Santa Barbara (CA), USA
  • Steven Porter
    • U. of Exeter, UK
  • Stéphane Mesnage
    • U. of Sheffield, UK
  • Marco Moracci
    • U. of Naples Federico II, IT
  • Giancarlo Parenti
    • U. of Naples Federico II, IT
  • Marta Artola, Hermen Overkleeft
    • Leiden University, NL
  • Ben Schumann
    • The Francis Crick Institute, London, UK

Funding