Bruno Canard
Directeur de recherche
Bruno Canard a obtenu un doctorat en 1991 en microbiologie et biochimie de l’Université Paris VII à l’Institut Pasteur (Dir : Dr. Stewart T. Cole), Paris, et une formation post-doctorale en 1995-1998 à la Harvard Medical School, Dept. Biological Chemistry and Molecular Pharmacology (Dir : Dr. Charles C. Richardson), Boston.
Il est actuellement responsable de l’équipe ” Replication Virale : Structure, Mécanismes, et Drug Design” au Laboratoire Architecture et Fonctions des Macromolécules Biologiques, Aix-Marseille Université, Marseille, France, où il occupe un poste de Directeur de Recherche CNRS.
Il s’intéresse actuellement à la structure et à la fonction des enzymes impliquées dans la réplication virale et la coiffe de l’ARN, et à leur utilisation dans la conception de médicaments antiviraux.
Publications
2022
A dual mechanism of action of AT-527 against SARS-CoV-2 polymerase
Ashleigh Shannon, Véronique Fattorini, Bhawna Sama, Barbara Selisko, Mikael Feracci, Camille Falcou, Pierre Gauffre, Priscila El Kazzi, Adrien Delpal, Etienne Decroly, Karine Alvarez, Cécilia Eydoux, Jean-Claude Guillemot, Adel Moussa, Steven Good, Paolo La Colla, Kai Lin, Jean-Pierre Sommadossi, Yingxiao Zhu, Xiaodong Yan, Hui Shi, Francois Ferron, Bruno Canard
Nature Communications 13 (2022)10.1038/s41467-022-28113-1
Fluoxetine targets an allosteric site in the enterovirus 2C AAA+ ATPase and stabilizes a ring-shaped hexameric complex
Daniel Hurdiss, Priscila El Kazzi, Lisa Bauer, Nicolas Papageorgiou, Francois Ferron, Tim Donselaar, Arno L.W. van Vliet, Tatiana Shamorkina, Joost Snijder, Bruno Canard, Etienne Decroly, Andrea Brancale, Tzviya Zeev-Ben-Mordehai, Friedrich Förster, Frank J.M. van Kuppeveld, Bruno Coutard
Science Advances 8 (2022)10.1126/sciadv.abj7615
Synthesis, Structure–Activity Relationships, and Antiviral Profiling of 1-Heteroaryl-2-Alkoxyphenyl Analogs As Inhibitors of SARS-CoV-2 Replication
Dorothée Bardiot, Laura Vangeel, Mohamed Koukni, Philippe Arzel, Marleen Zwaagstra, Heyrhyoung Lyoo, Patrick Wanningen, Shamshad Ahmad, Linlin Zhang, Xinyuanyuan Sun, Adrien Delpal, Cecilia Eydoux, Jean-Claude Guillemot, Eveline Lescrinier, Hugo Klaassen, Pieter Leyssen, Dirk Jochmans, Karolien Castermans, Rolf Hilgenfeld, Colin Robinson, Etienne Decroly, Bruno Canard, Eric Snijder, Martijn Hemert, Frank van Kuppeveld, Patrick Chaltin, Johan Neyts, Steven de Jonghe, Arnaud Marchand
Molecules 27:1052 (2022)10.3390/molecules27031052
2021
Structure-function analysis of the nsp14 N7-guanine methyltransferase reveals an essential role in Betacoronavirus replication
Natacha Ogando, Priscila El Kazzi, Jessika Zevenhoven-Dobbe, Brenda Bontes, Alice Decombe, Clara Posthuma, Volker Thiel, Bruno Canard, Francois Ferron, Etienne Decroly, Eric Snijder
Proceedings of the National Academy of Sciences of the United States of America (2021)10.1101/2021.05.17.444407
An appeal for an objective, open, and transparent scientific debate about the origin of SARS-CoV-2
Jacques van Helden, Colin Butler, Guillaume Achaz, Bruno Canard, Didier Casane, Jean-Michel Claverie, Fabien Colombo, Virginie Courtier-Orgogozo, Richard Ebright, François Graner, Milton Leitenberg, Serge Morand, Nikolai Petrovsky, Rossana Segreto, Etienne Decroly, José Halloy
The Lancet 398:1402-1404 (2021)10.1016/S0140-6736(21)02019-5
Structure and Sequence Requirements for RNA Capping at the Venezuelan Equine Encephalitis Virus RNA 5′ End
Oney Ortega Granda, Coralie Valle, Ashleigh Shannon, Etienne Decroly, Bruno Canard, Bruno Coutard, Nadia Rabah
Journal of Virology 95 (2021)10.1128/JVI.00777-21
The enzymes for genome size increase and maintenance of large (+)RNA viruses
Francois Ferron, Bhawna Sama, Etienne Decroly, Bruno Canard
Trends in Biochemical Sciences (2021)10.1016/j.tibs.2021.05.006
Simeprevir Potently Suppresses SARS-CoV-2 Replication and Synergizes with Remdesivir
Ho Sing Lo, Kenrie Pui Yan Hui, Hei-Ming Lai, Xu He, Khadija Shahed Khan, Simranjeet Kaur, Junzhe Huang, Zhongqi Li, Anthony Chan, Hayley Hei-Yin Cheung, Ka-Chun Ng, John Chi Wang Ho, Yu Wai Chen, Bowen Ma, Peter Man-Hin Cheung, Donghyuk Shin, Kaidao Wang, Meng-Hsuan Lee, Barbara Selisko, Cecilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Kuen-Phon Wu, Po-Huang Liang, Ivan Dikic, Zhong Zuo, Francis Chan, David Hui, Vincent Mok, Kam-Bo Wong, Chris Ka Pun Mok, Ho Ko, Wei Shen Aik, Michael Chi Wai Chan, Wai-Lung Ng
ACS Central Science 7:792-802 (2021)10.1021/acscentsci.0c01186
The methyltransferase domain of the Respiratory Syncytial Virus L protein catalyzes cap N7 and 2’-O-methylation
Priscila Sutto-Ortiz, Sergey Tcherniuk, Nina Ysebaert, Pravien Abeywickrema, Mathieu Noël, Alice Decombe, Françoise Debart, Jean-Jacques Vasseur, Bruno Canard, Dirk Roymans, Peter Rigaux, Jean-François Eléouët, Etienne Decroly
PLoS Pathogens 17:e1009562 (2021)10.1371/journal.ppat.1009562
Observation of arenavirus nucleoprotein heptamer assembly
Nicolas Papageorgiou, Afroditi Vaitsopoulou, Awa Diop, Thi van Nguyen, Bruno Canard, Karine Alvarez, Francois Ferron
FEBS Open Bio (2021)10.1002/2211-5463.13106
A fluorescence-based high throughput-screening assay for the SARS-CoV RNA synthesis complex
Cecilia Eydoux, Veronique Fattorini, Ashleigh Shannon, Thi-Tuyet-Nhung Le, Bruno Didier, Bruno Canard, Jean-Claude Guillemot
Journal of Virological Methods 288:114013 (2021)10.1016/j.jviromet.2020.114013
Dengue Virus NS5 Transcribes Metabolite-Capped, RIG-I Sensitive vRNAs
Brandon Schweibenz, Mihai Solotchi, Etienne Decroly, Barbara Selisko, Bruno Canard, Smita Patel
Biophysical Journal 120:135a (2021)10.1016/j.bpj.2020.11.1017
The methyltransferase domain of the Respiratory Syncytial Virus L protein catalyzes cap N7 and 2’-O-methylation
Priscila Sutto-Ortiz, Sergey Tcherniuk, Nina Ysebaert, Pravien Abeywickrema, Mathieu Noël, Alice Decombe, Françoise Debart, Jean-Jacques Vasseur, Bruno Canard, Dirk Roymans, Peter Rigaux, Jean-François Eléouët, Etienne Decroly
PLoS Pathogens 17:1-20 (2021)10.1371/journal.ppat.1009562
First insights into the structural features of Ebola virus methyltransferase activities
Coralie Valle, Baptiste Martin, François Ferron, Véronique Roig-Zamboni, Aline Desmyter, Françoise Debart, Jean Jacques Vasseur, Bruno Canard, Bruno Coutard, Etienne Decroly
Nucleic Acids Research 49:1737-1748 (2021)10.1093/nar/gkaa1276
The WHO mission report struggles to trace the origins of the SARS-CoV-2 epidemic
Etienne Decroly, Jean-Michel Claverie, Bruno Canard
Virologie 1-7 (2021)
Combining Antivirals and Immunomodulators to Fight COVID-19
Vincent Feuillet, Bruno Canard, Alain Trautmann
Trends in Immunology 42:31-44 (2021)10.1016/j.it.2020.11.003
2020
Synthesis and biological evaluation of novel flexible nucleoside analogues that inhibit flavivirus replication in vitro
Joy Thames, Charles Waters, Coralie Valle, Marcella Bassetto, Wahiba Aouadi, Baptiste Martin, Barbara Selisko, Arissa Falat, Bruno Coutard, Andrea Brancale, Bruno Canard, Etienne Decroly, Katherine Seley-Radtke
Bioorganic and Medicinal Chemistry Letters 28:115713 (2020)10.1016/j.bmc.2020.115713
Drugs against SARS‐CoV ‐2: What do we know about their mode of action?
Coralie Valle, Baptiste Martin, Franck Touret, Ashleigh Shannon, Bruno Canard, Jean‐claude Guillemot, Bruno Coutard, Etienne Decroly
Reviews in Medical Virology 30:1-10 (2020)10.1002/rmv.2143
Mutations on VEEV nsP1 relate RNA capping efficiency to ribavirin susceptibility
Nadia Rabah, Oney Ortega Granda, Gilles Quérat, Bruno Canard, Etienne Decroly, Bruno Coutard
Antiviral Research 182:104883 (2020)10.1016/j.antiviral.2020.104883
Rapid incorporation of Favipiravir by the fast and permissive viral RNA polymerase complex results in SARS-CoV-2 lethal mutagenesis
Ashleigh Shannon, Barbara Selisko, Nhung-Thi-Tuyet Le, Johanna Huchting, Franck Touret, Géraldine Piorkowski, Véronique Fattorini, Francois Ferron, Etienne Decroly, Chris Meier, Bruno Coutard, Olve Peersen, Bruno Canard
Nature Communications 11:4682 (2020)10.1038/s41467-020-18463-z
Snapshots of ADp-ribose bound to Getah virus macro domain reveal an intriguing choreography
Ana Ferreira-Ramos, Gerlind Sulzenbacher, Bruno Canard, Bruno Coutard
Scientific Reports 10 (2020)10.1038/s41598-020-70870-w
Synthesis of adenine dinucleosides SAM analogs as specific inhibitors of SARS-CoV nsp14 RNA cap guanine-N7-methyltransferase
Rostom Ahmed-Belkacem, Priscila Sutto-Ortiz, Mathis Guiraud, Bruno Canard, Jean-Jacques Vasseur, Etienne Decroly, Françoise Debart
European Journal of Medicinal Chemistry 201:112557 (2020)10.1016/j.ejmech.2020.112557
Brothers in Arms: Structure, Assembly and Function of Arenaviridae Nucleoprotein
Nicolas Papageorgiou, Maria Spiliopoulou, Thi-Hong van Nguyen, Afroditi Vaitsopoulou, Elsie Laban, Karine Alvarez, Irene Margiolaki, Bruno Canard, Francois Ferron
Viruses 12:772 (2020)10.3390/v12070772
Remdesivir and SARS-CoV-2: Structural requirements at both nsp12 RdRp and nsp14 Exonuclease active-sites
Ashleigh Shannon, Nhung Thi-Tuyet Le, Barbara Selisko, Cecilia Eydoux, Karine Alvarez, Jean-Claude Guillemot, Etienne Decroly, Olve Peersen, Francois Ferron, Bruno Canard
Antiviral Research 178:104793 (2020)10.1016/j.antiviral.2020.104793
A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae
Francois Ferron, Humberto Debat, Ashleigh Shannon, Etienne Decroly, Bruno Canard
NAR Genomics and Bioinformatics 2 (2020)10.1093/nargab/lqz022
Design, Synthesis and Discovery of N,N’ ‐Carbazoyl‐aryl‐urea Inhibitors of Zika NS5 Methyltransferase and Virus Replication
Sharon Spizzichino, Giulio Mattedi, Kate Lauder, Coralie Valle, Wahiba Aouadi, Bruno Canard, Etienne Decroly, Suzanne Kaptein, Johan Neyts, Carl Graham, Zakary Sule, David Barlow, Romano Silvestri, Daniele Castagnolo
ChemMedChem 15:385-390 (2020)10.1002/cmdc.201900533
The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade
Bruno Coutard, Coralie Valle, Xavier de Lamballerie, Bruno Canard, Nabil Seidah, Etienne Decroly
Antiviral Research 176:104742 (2020)10.1016/j.antiviral.2020.104742
The C-Terminal Domain of the Sudan Ebolavirus L Protein Is Essential for RNA Binding and Methylation
Coralie Valle, Baptiste Martin, Françoise Debart, Jean-Jacques Vasseur, Isabelle Imbert, Bruno Canard, Bruno Coutard, Etienne Decroly
Journal of Virology 94:e00520-20 (2020)10.1128/JVI.00520-20
2019
The Curious Case of the Nidovirus Exoribonuclease: Its Role in RNA Synthesis and Replication Fidelity
Natacha Ogando, Francois Ferron, Etienne Decroly, Bruno Canard, Clara Posthuma, Eric Snijder
Frontiers in Microbiology 10 (2019)10.3389/fmicb.2019.01813
Deciphering the Nucleotide and RNA Binding Selectivity of the Mayaro Virus Macro Domain
Aikaterini Tsika, Efstathios Melekis, Sofia-Antigoni Tsatsouli, Nicolas Papageorgiou, Maria Mate, Bruno Canard, Bruno Coutard, Detlef Bentrop, Georgios Spyroulias
Journal of Molecular Biology 431:2283-2297 (2019)10.1016/j.jmb.2019.04.013
Conformational plasticity of the VEEV macro domain is important for binding of ADP-ribose
Garyfallia Makrynitsa, Dioni Ntonti, Konstantinos Marousis, Maria Birkou, Minos-Timotheos Matsoukas, Sam Asami, Detlef Bentrop, Nicolas Papageorgiou, Bruno Canard, Bruno Coutard, Georgios Spyroulias
Journal of Structural Biology 206:119-127 (2019)10.1016/j.jsb.2019.02.008
Gln151 of HIV-1 reverse transcriptase acts as a steric gate towards clinically relevant acyclic phosphonate nucleotide analogues.
Antoine Frangeul, Cécile Bussetta, Jérôme Deval, Karine Barral, Karine Alvarez, Bruno Canard
Antiviral Therapy 13:115-24 (2019)
C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
Philippe Jaïs, Etienne Decroly, Eric Jacquet, Marine Le boulch, Aurélien Jaïs, Olivier Jean-Jean, Heather Eaton, Prishila Ponien, Frédérique Verdier, Bruno Canard, Sergio Gonçalves, Stéphane Chiron, Maude Le Gall, Patrick Mayeux, Maya Shmulevitz
Nucleic Acids Research 47:2681-2698 (2019)10.1093/nar/gkz069
Metal chelators for the inhibition of the lymphocytic choriomeningitis virus endonuclease domain
Magali Saez-Ayala, Elsie Laban Yekwa, Clémence Mondielli, Loic Roux, Sergio Hernández, Fabrice Bailly, Philippe Cotelle, Dominga Rogolino, Bruno Canard, Francois Ferron, Karine Alvarez
Antiviral Research 162:79-89 (2019)10.1016/j.antiviral.2018.12.008
Approved drugs screening against the nsP1 capping enzyme of Venezuelan equine encephalitis virus using an immuno-based assay
Ana Ferreira-Ramos, Changqing Li, Cecilia Eydoux, Jean Marie Contreras, Christophe Morice, Gilles Querat, Alba Gigante, María-Jesús Pérez Pérez, Marie-Louise Jung, Bruno Canard, Jean-Claude Guillemot, Etienne Decroly, Bruno Coutard
Antiviral Research 163:59-69 (2019)10.1016/j.antiviral.2019.01.003
Structure and oligomerization state of the C-terminal region of the Middle East respiratory syndrome coronavirus nucleoprotein
Thi Hong Van Nguyen, Julie Lichiere, Bruno Canard, Nicolas Papageorgiou, Sarah Attoumani, Francois Ferron, Bruno Coutard
Acta crystallographica Section D : Structural biology [1993-...] 75:8-15 (2019)10.1107/S2059798318014948
2018
Ebola virus L protein harbors a new enzymatic activity involved in the internal methylation of RNAs
Baptiste Martin, Coralie Valle, Bruno Coutard, Bruno Canard, Françoise Debart, Etienne Decroly
médecine/sciences 34:919-921 (2018)10.1051/medsci/2018230
The methyltransferase domain of the Sudan ebolavirus L protein specifically targets internal adenosines of RNA substrates, in addition to the cap structure
Baptiste Martin, Bruno Coutard, Théo Guez, Guido Paesen, Bruno Canard, Françoise Debart, Jean-Jacques Vasseur, Jonathan Grimes, Etienne Decroly
Nucleic Acids Research 46:7902-7912 (2018)10.1093/nar/gky637
Crystal structures of Lymphocytic choriomeningitis virus endonuclease domain complexed with diketo-acid ligands
Magali Saez-Ayala, Elsie Laban Yekwa, Mauro Carcelli, Bruno Canard, Karine Alvarez, Francois Ferron
International Union of Crystallography journal 5:223-235 (2018)10.1107/S2052252518001021
Crystal structures ofLymphocytic choriomeningitis virusendonuclease domain complexed with diketo-acid ligands
Magali Saez-Ayala, Elsie Yekwa, Mauro Carcelli, Bruno Canard, Karine Alvarez, Francois Ferron
International Union of Crystallography journal 5:223-235 (2018)10.1107/s2052252518001021
Structural and Functional Basis of the Fidelity of Nucleotide Selection by Flavivirus RNA-Dependent RNA Polymerases
Barbara Selisko, Nicolas Papageorgiou, Francois Ferron, Bruno Canard
Viruses 10:59 (2018)10.3390/v10020059
Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA
Francois Ferron, Lorenzo Subissi, Ana Theresa Silveira de Morais, Nhung Thi Tuyet Le, Marion Sevajol, Laure Gluais, Etienne Decroly, Clemens Vonrhein, Gérard Bricogne, Bruno Canard, Isabelle Imbert
Proceedings of the National Academy of Sciences of the United States of America 115:E162-E171 (2018)10.1073/pnas.1718806115
2017
Toward the identification of viral cap-methyltransferase inhibitors by fluorescence screening assay
Wahiba Aouadi, C Ecilia Eydoux, Bruno Coutard, Baptiste Martin, Françoise Debart, Jean-Jacques Vasseur, Jean Contreras, Christophe Morice, Gilles Quérat, Marie-Louise Jung, Bruno Canard, Jean-Claude Guillemot, Etienne Decroly
Antiviral Research 144:330-339 (2017)10.1016/j.antiviral.2017.06.021
Toscana virus nucleoprotein oligomer organization observed in solution
Amal Baklouti, Adeline Goulet, Julie Lichiere, Bruno Canard, Remi Charrel, Francois Ferron, Bruno Coutard, Nicolas Papageorgiou
Acta crystallographica Section D : Structural biology [1993-...] 73:650-659 (2017)10.1107/S2059798317008774
Activity inhibition and crystal polymorphism induced by active-site metal swapping
Elsie Yekwa, Joelle Khourieh, Bruno Canard, Nicolas Papageorgiou, Francois Ferron
Acta crystallographica Section D : Structural biology [1993-...] 73:641-649 (2017)10.1107/S205979831700866X
Understanding the Mechanism of the Broad-Spectrum Antiviral Activity of Favipiravir (T-705): Key Role of the F1 Motif of the Viral Polymerase
Rana Abdelnabi, Ana Theresa Silveira De Morais, Pieter Leyssen, Isabelle Imbert, Stéphanie Beaucourt, Hervé Blanc, Mathy Froeyen, Marco Vignuzzi, Bruno Canard, Johan Neyts, Leen Delang
Journal of Virology 91:e00487-17 (2017)10.1128/JVI.00487-17
Biochemical principles and inhibitors to interfere with viral capping pathways
Etienne Decroly, Bruno Canard
Current Opinion in Virology 24:87-96 (2017)10.1016/j.coviro.2017.04.003
Filovirus proteins for antiviral drug discovery: Structure/function bases of the replication cycle
Baptiste Martin, Bruno Canard, Etienne Decroly
Antiviral Research 141:48-61 (2017)10.1016/j.antiviral.2017.02.004
Substrate selectivity of Dengue and Zika virus NS5 polymerase towards 2′-modified nucleotide analogues
Supanee Potisopon, Francois Ferron, Véronique Fattorini, Barbara Selisko, Bruno Canard
Antiviral Research 140:25-36 (2017)10.1016/j.antiviral.2016.12.021
Binding of the Methyl Donor S -Adenosyl-l-Methionine to Middle East Respiratory Syndrome Coronavirus 2′- O -Methyltransferase nsp16 Promotes Recruitment of the Allosteric Activator nsp10
Wahiba Aouadi, Alexandre Blanjoie, Jean-Jacques Vasseur, Françoise Debart, Bruno Canard, Etienne Decroly
Journal of Virology 91:1103-1119 (2017)10.1128/JVI.02217-16
Zika Virus Methyltransferase: Structure and Functions for Drug Design Perspectives
Bruno Coutard, Karine Barral, Julie Lichiere, Barbara Selisko, Baptiste Martin, Wahiba Aouadi, Miguel Ortiz Lombardia, Françoise Debart, Jean-Jacques Vasseur, Jean Claude Guillemot, Bruno Canard, Etienne Decroly
Journal of Virology 91:2202-2218 (2017)10.1128/JVI.02202-16
2016
Coxsackievirus B3 protease 3C: expression, purification, crystallization and preliminary structural insights
Stavroula Fili, Alexandros Valmas, Magdalini Christopoulou, Maria Spiliopoulou, Nikos Nikolopoulos, Julie Lichiere, Souzana Logotheti, Fotini Karavassili, Eleftheria Rosmaraki, Andrew Fitch, Jonathan Wright, Detlef Beckers, Thomas Degen, Gwilherm Nenert, Rolf Hilgenfeld, Nicolas Papageorgiou, Bruno Canard, Bruno Coutard, Irene Margiolaki
Acta crystallographica Section F : Structural biology communications [2014-...] 72:877-884 (2016)10.1107/S2053230X16018513
Filovirus proteins for antiviral drug discovery: A structure/function analysis of surface glycoproteins and virus entry
Baptiste Martin, Thomas Hoenen, Bruno Canard, Etienne Decroly
Antiviral Research 135:1-14 (2016)10.1016/j.antiviral.2016.09.001
Viral Macro Domains Reverse Protein ADP-Ribosylation
Changqing Li, Yannick Debing, Gytis Jankevicius, Johan Neyts, Ivan Ahel, Bruno Coutard, Bruno Canard
Journal of Virology 90:8478-8486 (2016)10.1128/JVI.00705-16
Screening of a Library of FDA-Approved Drugs Identifies Several Enterovirus Replication Inhibitors That Target Viral Protein 2C
Rachel Ulferts, S. Matthijn Boer, Lonneke Linden, Lisa Bauer, Hey Rhyoung Lyoo, Maria J. Mate, Julie Lichiere, Bruno Canard, Daphne Lelieveld, Wienand Omta, David Egan, Bruno Coutard, Frank J. M. Kuppeveld
Antimicrobial Agents and Chemotherapy 60:2627-2638 (2016)10.1128/AAC.02182-15
The methyltransferase domain of dengue virus protein NS5 ensures efficient RNA synthesis initiation and elongation by the polymerase domain (vol 42, pg 11642, 2014)
Supanee Potisopon, Stephane Priet, Axelle Collet, Etienne Decroly, Bruno Canard, Barbara Selisko
Nucleic Acids Research 44:2974 (2016)10.1093/nar/gkv1294
Structural characterization of the N-terminal part of the MERS-CoV nucleocapsid by X-ray diffraction and small-angle X-ray scattering
Nicolas Papageorgiou, Julie Lichiere, Amal Baklouti, Francois Ferron, Marion Sevajol, Bruno Canard, Bruno Coutard
Acta crystallographica Section D : Structural biology [1993-...] 72:192-202 (2016)10.1107/S2059798315024328
Novel 2-phenyl-5-[(E)-2-(thiophen-2-yl)ethenyl]-1,3,4-oxadiazole and 3-phenyl-5-[(E)-2-(thiophen-2-yl)ethenyl]-1,2,4-oxadiazole derivatives as dengue virus inhibitors targeting NS5 polymerase
Fatiha Benmansour, Cecilia Eydoux, Gilles Querat, Xavier de Lamballerie, Bruno Canard, Karine Alvarez, Jean-Claude Guillemot, Karine Barral
European Journal of Medicinal Chemistry 109:146-156 (2016)10.1016/j.ejmech.2015.12.046
2015
mRNA Capping by Venezuelan Equine Encephalitis Virus nsP1: Functional Characterization and Implications for Antiviral Research
Changqing Li, Jaime Guillen, Nadia Rabah, Alexandre Blanjoie, Francoise Debart, Jean-Jacques Vasseur, Bruno Canard, Etienne Decroly, Bruno Coutard
Journal of Virology 89:8292-8303 (2015)10.1128/JVI.00599-15
Enzymatic synthesis of acyclic nucleoside thiophosphonate diphosphates: Effect of the alpha-phosphorus configuration on HIV-1 RT activity
Stephane Priet, Loic Roux, Magali Saez-Ayala, Francois Ferron, Bruno Canard, Karine Alvarez
Antiviral Research 117:122-131 (2015)10.1016/j.antiviral.2015.03.003
Structural and biophysical analysis of sequence insertions in the Venezuelan Equine Encephalitis Virus macro domain
Jaime Guillen, Julie Lichiere, Nadia Rabah, Brett F. Beitzel, Bruno Canard, Bruno Coutard
Virus Research 201:94-100 (2015)10.1016/j.virusres.2015.02.018
Primer-Dependent and Primer-Independent Initiation of Double Stranded RNA Synthesis by Purified Arabidopsis RNA-Dependent RNA Polymerases RDR2 and RDR6
Anthony Devert, Nicolas Fabre, Maïna Floris, Bruno Canard, Christophe Robaglia, Patrice Crété
PLoS ONE 10 (2015)10.1371/journal.pone.0120100.s006
The RNA Template Channel of the RNA-Dependent RNA Polymerase as a Target for Development of Antiviral Therapy of Multiple Genera within a Virus Family
Lonneke van der Linden, Laia Vives-Adrián, Barbara Selisko, Cristina Ferrer-Orta, Xinran Liu, Kjerstin Lanke, Rachel Ulferts, Armando M. de Palma, Federica Tanchis, Nesya Goris, David Lefebvre, Kris de Clercq, Pieter Leyssen, Céline Lacroix, Gerhard Pürstinger, Bruno Coutard, Bruno Canard, David D. Boehr, Jamie J. Arnold, Craig E. Cameron, Nuria Verdaguer, Johan Neyts, Frank J. M van Kuppeveld
PLoS Pathogens 11 (2015)10.1371/journal.ppat.1004733.s010
X-ray structure and activities of an essential Mononegavirales L-protein domain
Guido C. Paesen, Axelle Collet, Corinne Sallamand, Francoise Debart, Jean-Jacques Vasseur, Bruno Canard, Etienne Decroly, Jonathan M. Grimes
Nature Communications 6:8749 (2015)10.1038/ncomms9749
2014
The methyltransferase domain of dengue virus protein NS5 ensures efficient RNA synthesis initiation and elongation by the polymerase domain
Supanee Potisopon, Stéphane Priet, Axelle Collet, Etienne Decroly, Bruno Canard, Barbara Selisko
Nucleic Acids Research 42 (2014)10.1093/nar/gku666
Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes
Mickaël Bouvet, Adrien Lugari, Clara C. Posthuma, Jessika C. Zevenhoven, Stéphanie Bernard, Stéphane Betzi, Isabelle Imbert, Bruno Canard, Jean-Claude Guillemot, Patrick Lécine, Susanne Pfefferle, Christian Drosten, Eric J. Snijder, Etienne Decroly, Xavier Morelli
Journal of Biological Chemistry 289:25783-25796 (2014)10.1074/jbc.M114.577353
2013
Development of specific dengue virus 2'-O- and N7-methyltransferase assays for antiviral drug screening.
Karine Barral, Corinne Sallamand, Christiane Petzold, Bruno Coutard, Axelle Collet, Yann Thillier, Jan Zimmermann, Jean-Jacques Vasseur, Bruno Canard, Jacques Rohayem, Françoise Debart, Etienne Decroly
Antiviral Research 99:292-300 (2013)10.1016/j.antiviral.2013.06.001
2012
Antiviral chlorinated daphnane diterpenoid orthoesters from the bark and wood of Trigonostemon cherrieri.
Pierre-Marie Allard, Pieter Leyssen, Marie-Thérèse Martin, Mélanie Bourjot, Vincent Dumontet, Cécilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Cyril Poullain, Françoise Guéritte, Marc Litaudon
Phytochemistry 84:160-8 (2012)10.1016/j.phytochem.2012.07.023
Chemical constituents of Anacolosa pervilleana and their antiviral activities.
Mélanie Bourjot, Pieter Leyssen, Cécilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Philippe Rasoanaivo, Françoise Guéritte, Marc Litaudon
Fitoterapia 83:1076-1080 (2012)10.1016/j.fitote.2012.05.004
Flacourtosides A-F, Phenolic Glycosides Isolated from Flacourtia ramontchi.
Mélanie Bourjot, Pieter Leyssen, Cécilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Philippe Rasoanaivo, Françoise Guéritte, Marc Litaudon
Journal of Natural Products 75:752-8 (2012)10.1021/np300059n
Molecular Basis for Nucleotide Conservation at the Ends of the Dengue Virus Genome
Barbara Selisko, Supanee Potisopon, Rym Agred, Stephane Priet, Isabelle Varlet, Yann Thillier, Corinne Sallamand, Françoise Debart, Jean-Jacques Vasseur, Bruno Canard
PLoS Pathogens 8:e1002912 (2012)10.1371/journal.ppat.1002912
Single pH buffer refolding screen for protein from inclusion bodies
Bruno Coutard, Etienne Danchin, Rachid Oubelaid, Bruno Canard, Christophe Bignon
Protein Expression and Purification 82:352-359 (2012)10.1016/j.pep.2012.01.014
Synthesis of 5' cap-0 and cap-1 RNAs using solid-phase chemistry coupled with enzymatic methylation by human (guanine-N 7)-methyl transferase
Yann Thillier, Etienne Decroly, François Morvan, Bruno Canard, Jean-Jacques Vasseur, Françoise Debart
RNA 18:856-868 (2012)10.1261/rna.030932.111
2011
Alkylated flavanones from the bark of Cryptocarya chartacea as dengue virus NS5 polymerase inhibitors.
Pierre-Marie Allard, Elise Tran Huu Dau, Cécilia Eydoux, Jean-Claude Guillemot, Vincent Dumontet, Cyril Poullain, Bruno Canard, Françoise Guéritte, Marc Litaudon
Journal of Natural Products 74:2446-53 (2011)10.1021/np200715v
Acyclic nucleoside thiophosphonates as potent inhibitors of HIV and HBV replication
Karine Barral, Clément Weck, Nadine Payrot, Loic Roux, Céline Durafour, Fabien Zoulim, Johan Neyts, Jan Balzarini, Bruno Canard, Stephane Priet, Karine Alvarez
European Journal of Medicinal Chemistry 46:4281-4288 (2011)10.1016/j.ejmech.2011.06.034
Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex.
Claire Debarnot, Isabelle Imbert, Francois Ferron, Laure Gluais, Isabelle Varlet, Nicolas Papageorgiou, Mickaël Bouvet, Julien Lescar, Etienne Decroly, Bruno Canard
Acta crystallographica Section F : Structural biology communications [2014-...] 67:404-8 (2011)10.1107/S1744309111002867
Flavivirus NS3 and NS5 proteins interaction network: a high-throughput yeast two-hybrid screen.
Marc Le Breton, Laurène Meyniel-Schicklin, Alexandre Deloire, Bruno Coutard, Bruno Canard, Xavier de Lamballerie, Patrice Andre, Chantal Rabourdin-Combe, Vincent Lotteau, Nathalie Davoust
BMC Microbiology 11:234 (2011)10.1186/1471-2180-11-234
PHYTOCHIK: Biodiversity As A Source of Selective Inhibitors of CHIKV Replication
Pieter Leyssen, Marc Litaudon, Jean-Claude Guillemot, Philippe Rasoanaivo, Jacqueline Smadja, Ameenah Gurib-Fakim, Bruno Canard, Françoise Guéritte
Antiviral Research 90:A36-A37 (2011)10.1016/j.antiviral.2011.03.048
2010
Genomics and structure/function studies of Rhabdoviridae proteins involved in replication and transcription.
R. Assenberg, O Delmas, B Morin, C Graham, X de Lamballerie, C Laubert, B Coutard, J Grimes, J Neyts, R J Owens, B Brandt, A Gorbalenya, P Tucker, D I Stuart, Bruno Canard, Hervé Bourhy
Antiviral Research 87:149-61 (2010)10.1016/j.antiviral.2010.02.322
Synthesis and antiviral activity of boranophosphonate isosteres of AZT and d4T monophosphates
Karine Barral, Stephane Priet, Céline de Michelis, Joséphine Sire, Johan Neyts, Jan Balzarini, Bruno Canard, Karine Alvarez
European Journal of Medicinal Chemistry 45:849-856 (2010)10.1016/j.ejmech.2009.11.012
3'-Deoxy phosphoramidate dinucleosides as improved inhibitors of Hepatitis C virus subgenomic replicon and NS5B polymerase activity.
Stephane Priet, Ivan Zlatev, Ivan Barvik, Katrien Geerts, Pieter Leyssen, Johan Neyts, Hélène Dutartre, Bruno Canard, Jean-Jacques Vasseur, François Morvan, Karine Alvarez
Journal of Medicinal Chemistry 53:6608-6617 (2010)10.1021/jm100102v
2009
Identification of allosteric inhibitors blocking the hepatitis C virus polymerase NS5B in the RNA synthesis initiation step.
Stéphane Betzi, Cécilia Eydoux, Cécile Bussetta, Marilyne Blemont, Pieter Leyssen, Claire Debarnot, Mohamed Ben-Rahou, Jacques Haiech, Marcel Hibert, Françoise Gueritte, David Grierson, Jean-Louis Romette, Jean-Claude Guillemot, Johan Neyts, Karine Alvarez, Xavier Morelli, Hélène Dutartre, Bruno Canard
Antiviral Research 84:48-59 (2009)10.1016/j.antiviral.2009.06.009
A pyrazolotriazolopyrimidinamine inhibitor of bovine viral diarrhea virus replication that targets the viral RNA-dependent RNA polymerase
Jan Paeshuyse, Carine Letellier, Matheus Froeyen, Hélène Dutartre, Robert Vrancken, Bruno Canard, Erik de Clercq, Alain Gueiffier, Jean-Claude Teulade, Piet Herdewijn, Gerhard Puerstinger, Frank Koenen, Pierre Kerkhofs, Pier Giovanni Baraldi, Johan Neyts
Antiviral Research 82:141-147 (2009)10.1016/j.antiviral.2009.02.192
2007
The Imidazopyrrolopyridine Analogue AG110 Is a Novel, Highly Selective Inhibitor of Pestiviruses That Targets the Viral RNA-Dependent RNA Polymerase at a Hot Spot for Inhibition of Viral Replication
Jan Paeshuyse, Jean-Michel Chezal, Matheus Froeyen, Pieter Leyssen, Hélène Dutartre, Robert Vrancken, Bruno Canard, Carine Letellier, Tong Li, Harald Mittendorfer, Frank Koenen, Pierre Kerkhofs, Erik de Clercq, Piet Herdewijn, Gerhard Puerstinger, Alain Gueiffier, Olivier Chavignon, Jean-Claude Teulade, Johan Neyts
Journal of Virology 81:11046-11053 (2007)10.1128/JVI.00388-07
Crystal Structure of the RNA Polymerase Domain of the West Nile Virus Non-structural Protein 5
Hélène Malet, Marie-Pierre Egloff, Barbara Selisko, Rebecca Butcher, Peter Wright, Michael Roberts, Arnaud Gruez, Gerlind Sulzenbacher, Clemens Vonrhein, Gérard Bricogne, Jason Mackenzie, Alexander Khromykh, Andrew Davidson, Bruno Canard
Journal of Biological Chemistry (2007)10.1074/jbc.M607273200
2006
Synthesis, in Vitro Antiviral Evaluation, and Stability Studies of Novel α-Borano-Nucleotide Analogues of 9-[2-(Phosphonomethoxy)ethyl]adenine and ( R )-9-[2-(Phosphonomethoxy)propyl]adenine
Karine Barral, Stephane Priet, Joséphine Sire, Johan Neyts, Jan Balzarini, Bruno Canard, Karine Alvarez
Journal of Medicinal Chemistry 49:7799-7806 (2006)10.1021/jm060030y
General Catalytic Deficiency of Hepatitis C Virus RNA Polymerase with an S282T Mutation and Mutually Exclusive Resistance towards 2'-Modified Nucleotide Analogues
Hélène Dutartre, Cécile Bussetta, Joëlle Boretto, Bruno Canard
Antimicrobial Agents and Chemotherapy 50:4161-4169 (2006)10.1128/AAC.00433-06
Structural and Functional Basis for ADP-Ribose and Poly(ADP-Ribose) Binding by Viral Macro Domains
Marie-Pierre Egloff, Hélène Malet, Ákos Putics, Maarit Heinonen, Hélène Dutartre, Antoine Frangeul, Arnaud Gruez, Valérie Campanacci, Christian Cambillau, John Ziebuhr, Tero Ahola, Bruno Canard
Journal of Virology 80:8493-8502 (2006)10.1128/JVI.00713-06
Comparative mechanistic studies of de novo RNA synthesis by flavivirus RNA-dependent RNA polymerases
Barbara Selisko, Hélène Dutartre, Jean-Claude Guillemot, Claire Debarnot, Delphine Benarroch, Alexander Khromykh, Philippe Desprès, Marie-Pierre Egloff, Bruno Canard
Virology 351:145-158 (2006)10.1016/j.virol.2006.03.026
Synthesis and Anti-BVDV Activity of Acridones As New Potential Antiviral Agents 1
Oriana Tabarrini, Giuseppe Manfroni, Arnaldo Fravolini, Violetta Cecchetti, Stefano Sabatini, Erik de Clercq, Jef Rozenski, Bruno Canard, Hélène Dutartre, Jan Paeshuyse, Johan Neyts
Journal of Medicinal Chemistry 49:2621-2627 (2006)10.1021/jm051250z
Expression, purification and crystallization of the SARS-CoV macro domain
Hélène Malet, Karen Dalle, Nicolas Bremond, Fabienne Tocque, Stéphanie Blangy, Valérie Campanacci, Bruno Coutard, Sacha Grisel, Julie Lichiere, Violaine Lantez, Christian Cambillau, Bruno Canard, Marie-Pierre Egloff
Acta Crystallographica Section F: Structural Biology and Crystallization Communications 62:405-408 (2006)10.1107/S1744309106009274
2005
A Novel, Highly Selective Inhibitor of Pestivirus Replication That Targets the Viral RNA-Dependent RNA Polymerase
Jan Paeshuyse, Pieter Leyssen, Eric Mabery, Nina Boddeker, Robert Vrancken, Matheus Froeyen, Israrul Ansari, Hélène Dutartre, Jef Rozenski, Laura Gil, Carine Letellier, Robert Lanford, Bruno Canard, Frank Koenen, Pierre Kerkhofs, Ruben Donis, Piet Herdewijn, Julia Watson, Erik de Clercq, Gerhard Puerstinger, Johan Neyts
Journal of Virology 80:149-160 (2005)10.1128/JVI.80.1.149-160.2006
The modeled structure of the RNA dependent RNA polymerase of GBV-C virus suggests a role for motif E in Flaviviridae RNA polymerases.
Francois Ferron, Cécile Bussetta, Hélène Dutartre, Bruno Canard
BMC Bioinformatics 6:255 (2005)10.1186/1471-2105-6-255
Borano-nucleotides: new analogues to circumvent HIV-1 RT-mediated nucleoside drug-resistance
Karine Alvarez, Jérôme Deval, Boulbaba Selmi, Karine Barral, Joelle Boretto, Catherine Guerreiro, Laurence Mulard, Robert Sarfati, Bruno Canard
Nucleosides, Nucleotides and Nucleic Acids 24:419-422 (2005)10.1081/NCN-200059951
A Relaxed Discrimination of 2′- O -Methyl-GTP Relative to GTP between de Novo and Elongative RNA Synthesis by the Hepatitis C RNA-dependent RNA Polymerase NS5B
Hélène Dutartre, Joëlle Boretto, Jean Claude Guillemot, Bruno Canard
Journal of Biological Chemistry 280:6359-6368 (2005)10.1074/jbc.M410191200
2004
The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world
Marie-Pierre Egloff, Francois Ferron, Valérie Campanacci, Sonia Longhi, Corinne Rancurel, Hélène Dutartre, Eric Snijder, Alexander Gorbalenya, Christian Cambillau, Bruno Canard
Proceedings of the National Academy of Sciences of the United States of America 101:3792-3796 (2004)10.1073/pnas.0307877101
2003
Structural Analysis of the Activation of Ribavirin Analogs by NDP Kinase: Comparison with Other Ribavirin Targets
Sarah Gallois-Montbrun, Yuxing Chen, Hélène Dutartre, Magali Sophys, Solange Moréra, Catherine Guerreiro, Benoit Schneider, Laurence Mulard, Joël Janin, Michel Véron, Dominique Deville-Bonne, Bruno Canard
Molecular Pharmacology 63:538-546 (2003)10.1124/mol.63.3.538
1992
Phylogenetic analysis of the pathogenic anaerobe Clostridium perfringens using the 16S rRNA nucleotide sequence.
Bruno Canard, Thierry Garnier, Bénédicte Lafay, Richard Christen, Stewart T. Cole
International Journal of Systematic Bacteriology 42:312-4 (1992)10.1099/00207713-42-2-312
