2007-2022 : Research Engineer (AFMB-UMR7257)

Project : Drug-design against emerging virus. Recombinant proteins production/purification in bacteria. In vitro RNA synthesis. Enzymatic assays (radioactive or fluorescent) on viral RNA polymerases (Dengue,Zika,HCV,Coronavirus…). Compounds screening and in vitro inhibition study. Development of new technologies (fluorescent assays (HTRF…) or radioactive, robotization, miniaturization). Data base management. Student management.

2004-2007 : PHD (EIPL-UPR9025 Marseille)

Project : Biochemical,structural and Physiopathological studies of the human pancreatic lipase related protein 2 (HPLRP2). Recombinant proteins production/purification in yeast. Determination of biochemical, enzymatical and physiological properties. Immuno-histological studies on tissus, liquids and human biopsies. HPLRP2 cristallization. In vitro inhibition studies.

Publications

2022

2022
A dual mechanism of action of AT-527 against SARS-CoV-2 polymerase

Ashleigh Shannon, Véronique Fattorini, Bhawna Sama, Barbara Selisko, Mikael Feracci, Camille Falcou, Pierre Gauffre, Priscila El Kazzi, Adrien Delpal, Etienne Decroly, Karine Alvarez, Cécilia Eydoux, Jean-Claude Guillemot, Adel Moussa, Steven S Good, Paolo La Colla, Kai Lin, Jean-Pierre Sommadossi, Yingxiao Zhu, Xiaodong Yan, Hui Shi, Francois Ferron, Bruno Canard

Nature Communications 13 (2022)10.1038/s41467-022-28113-1

2022
Synthesis, Structure–Activity Relationships, and Antiviral Profiling of 1-Heteroaryl-2-Alkoxyphenyl Analogs As Inhibitors of SARS-CoV-2 Replication

Dorothée Bardiot, Laura Vangeel, Mohamed Koukni, Philippe Arzel, Marleen Zwaagstra, Heyrhyoung Lyoo, Patrick Wanningen, Shamshad Ahmad, Linlin Zhang, Xinyuanyuan Sun, Adrien Delpal, Cecilia Eydoux, Jean-Claude Guillemot, Eveline Lescrinier, Hugo Klaassen, Pieter Leyssen, Dirk Jochmans, Karolien Castermans, Rolf Hilgenfeld, Colin Robinson, Etienne Decroly, Bruno Canard, Eric J Snijder, Martijn J Van Hemert, Frank Van van Kuppeveld, Patrick Chaltin, Johan Neyts, Steven De de Jonghe, Arnaud Marchand

Molecules 27:1052 (2022)10.3390/molecules27031052

2021

2021
System-oriented optimization of multi-target 2,6-diaminopurine derivatives: Easily accessible broad-spectrum antivirals active against flaviviruses, influenza virus and SARS-CoV-2

Ilaria Vicenti, Maria Grazia Martina, Adele Boccuto, Marta de Angelis, Giorgia Giavarini, Filippo Dragoni, Serena Marchi, Claudia Maria Trombetta, Emmanuele Crespan, Giovanni Maga, Cecilia Eydoux, Etienne Decroly, Emanuele Montomoli, Lucia Nencioni, Maurizio Zazzi, Marco Radi

European Journal of Medicinal Chemistry 224:113683 (2021)10.1016/j.ejmech.2021.113683

2021
System-oriented optimization of multi-target 2,6-diaminopurine derivatives: Easily accessible broad-spectrum antivirals active against flaviviruses, influenza virus and SARS-CoV-2

Ilaria Vicenti, Maria Grazia Martina, Adele Boccuto, Marta de Angelis, Giorgia Giavarini, Filippo Dragoni, Serena Marchi, Claudia Maria Trombetta, Emmanuele Crespan, Giovanni Maga, Cecilia Eydoux, Etienne Decroly, Emanuele Montomoli, Lucia Nencioni, Maurizio Zazzi, Marco Radi

European Journal of Medicinal Chemistry 224:113683 (2021)10.1016/j.ejmech.2021.113683

2021
Simeprevir Potently Suppresses SARS-CoV-2 Replication and Synergizes with Remdesivir

Ho Sing Lo, Kenrie Pui yan Hui, Hei-Ming Lai, Xu He, Khadija Shahed Khan, Simranjeet Kaur, Junzhe Huang, Zhongqi Li, Anthony Chan, Hayley Hei-yin Cheung, Ka-Chun Ng, John Chi Wang Ho, yu Wai Chen, Bowen Ma, Peter Man-Hin Cheung, Donghyuk Shin, Kaidao Wang, Meng-Hsuan Lee, Barbara Selisko, Cecilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Kuen-Phon Wu, Po-Huang Liang, Ivan Dikic, Zhong Zuo, Francis Chan, David Hui, Vincent Mok, Kam-Bo Wong, Chris Ka Pun Mok, Ho Ko, Wei Shen Aik, Michael Chi Wai Chan, Wai-Lung Ng

ACS Central Science 7:792-802 (2021)10.1021/acscentsci.0c01186

2021
A fluorescence-based high throughput-screening assay for the SARS-CoV RNA synthesis complex

Cecilia Eydoux, Veronique Fattorini, Ashleigh Shannon, Thi-Tuyet-Nhung Le, Bruno Didier, Bruno Canard, Jean-Claude Guillemot

Journal of Virological Methods 288:114013 (2021)10.1016/j.jviromet.2020.114013

2020

2020
Remdesivir and SARS-CoV-2: Structural requirements at both nsp12 RdRp and nsp14 Exonuclease active-sites

Ashleigh Shannon, Nhung Thi-Tuyet Le, Barbara Selisko, Cecilia Eydoux, Karine Alvarez, Jean-Claude Guillemot, Etienne Decroly, Olve Peersen, Francois Ferron, Bruno Canard

Antiviral Research 178:104793 (2020)10.1016/j.antiviral.2020.104793

2020
Carneic Acids from an Endophytic Phomopsis sp. as Dengue Virus Polymerase Inhibitors

Laure-Anne Peyrat, Véronique Eparvier, Cecilia Eydoux, Jean-Claude Guillemot, Marc Litaudon, Didier Stien

Journal of Natural Products (2020)10.1021/acs.jnatprod.9b01169

2019

2019
Antiviral Compounds from Codiaeum peltatum Targeted by a Multi-informative Molecular Networks Approach

Florent Olivon, Simon Remy, Gwendal Grelier, Cécile Apel, Cecilia Eydoux, Jean-Claude Guillemot, Johan Neyts, Leen Delang, David Touboul, Fanny Roussi, Marc Litaudon

Journal of Natural Products 82:330-340 (2019)10.1021/acs.jnatprod.8b00800

2019
Approved drugs screening against the nsP1 capping enzyme of Venezuelan equine encephalitis virus using an immuno-based assay

Ana Ferreira-Ramos, Changqing Li, Cecilia Eydoux, Jean Marie Contreras, Christophe Morice, Gilles Querat, Alba Gigante, María-Jesús Pérez Pérez, Marie-Louise Jung, Bruno Canard, Jean-Claude Guillemot, Etienne Decroly, Bruno Coutard

Antiviral Research 163:59-69 (2019)10.1016/j.antiviral.2019.01.003

2017

2017
Betulinic Acid, The First Lupane-Type Triterpenoid Isolated from Both a Phomopsis sp. and Its Host Plant Diospyros carbonaria Benoist

Laure-Anne Peyrat, Véronique Eparvier, Cécilia Eydoux, Jean-Claude Guillemot, Marc Litaudon, Didier Stien

Chemistry & Biodiversity 14:e1600171 (2017)10.1002/cbdv.201600171

2016

2016
Protein-Protein Interaction Inhibition (2P21)-Oriented Chemical Library Accelerates Hit Discovery

Sabine Milhas, Brigitt Raux, Stéphane Betzi, Carine Derviaux, Philippe Roche, Audrey Restouin, Marie-Jeanne Basse, Etienne Rebuffet, Adrien Lugari, Marion Badol, Rudra Kashyap, Jean-Claude Lissitzky, Cecilia Eydoux, Veronique Hamon, Marie -Edith Gourde, Sebastien Combes, Pascale Zimmermann, Michel Aurrand-Lions, Thomas Roux, Catherine Rogers, Susanne Muller, Stefan Knapp, Eric Trinquet, Yves Collette, Jean-Claude Guillemot, Xavier Morelli

ACS Chemical Biology 11:2140-2148 (2016)10.1021/acschembio.6b00286

2016
Novel 2-phenyl-5-[(E)-2-(thiophen-2-yl)ethenyl]-1,3,4-oxadiazole and 3-phenyl-5-[(E)-2-(thiophen-2-yl)ethenyl]-1,2,4-oxadiazole derivatives as dengue virus inhibitors targeting NS5 polymerase

Fatiha Benmansour, Cecilia Eydoux, Gilles Querat, Xavier de Lamballerie, Bruno Canard, Karine Alvarez, Jean-Claude Guillemot, Karine Barral

European Journal of Medicinal Chemistry 109:146-156 (2016)10.1016/j.ejmech.2015.12.046

2016
Chemical diversity and antiviral potential in the pantropical Diospyros genus

Laure-Anne Peyrat, Véronique Eparvier, Cécilia Eydoux, Jean-Claude Guillemot, Didier Stien, Marc Litaudon

Fitoterapia (2016)10.1016/j.fitote.2016.04.017

2012

2012
Antiviral chlorinated daphnane diterpenoid orthoesters from the bark and wood of Trigonostemon cherrieri.

Pierre-Marie Allard, Pieter Leyssen, Marie-Thérèse Martin, Mélanie Bourjot, Vincent Dumontet, Cécilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Cyril Poullain, Françoise Guéritte, Marc Litaudon

Phytochemistry 84:160-8 (2012)10.1016/j.phytochem.2012.07.023

2012
Chemical constituents of Anacolosa pervilleana and their antiviral activities.

Mélanie Bourjot, Pieter Leyssen, Cécilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Philippe Rasoanaivo, Françoise Guéritte, Marc Litaudon

Fitoterapia 83:1076-1080 (2012)10.1016/j.fitote.2012.05.004

2012
Flacourtosides A-F, Phenolic Glycosides Isolated from Flacourtia ramontchi.

Mélanie Bourjot, Pieter Leyssen, Cécilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Philippe Rasoanaivo, Françoise Guéritte, Marc Litaudon

Journal of Natural Products 75:752-8 (2012)10.1021/np300059n

2011

2011
Alkylated flavanones from the bark of Cryptocarya chartacea as dengue virus NS5 polymerase inhibitors.

Pierre-Marie Allard, Elise Tran Huu Dau, Cécilia Eydoux, Jean-Claude Guillemot, Vincent Dumontet, Cyril Poullain, Bruno Canard, Françoise Guéritte, Marc Litaudon

Journal of Natural Products 74:2446-53 (2011)10.1021/np200715v

2009

2009
Identification of allosteric inhibitors blocking the hepatitis C virus polymerase NS5B in the RNA synthesis initiation step.

Stéphane Betzi, Cécilia Eydoux, Cécile Bussetta, Marilyne Blemont, Pieter Leyssen, Claire Debarnot, Mohamed Ben-Rahou, Jacques Haiech, Marcel Hibert, Françoise Gueritte, David S. Grierson, Jean-Louis Romette, Jean-Claude Guillemot, Johan Neyts, Karine Alvarez, Xavier Morelli, Hélène Dutartre, Bruno Canard

Antiviral Research 84:48-59 (2009)10.1016/j.antiviral.2009.06.009

2008

2008
Structure of Human Pancreatic Lipase-Related Protein 2 with the Lid in an Open Conformation ,

Cécilia Eydoux, Silvia Spinelli, Tara Davis, John Walker, Alma Seitova, Sirano Dhe-Paganon, Alain de Caro, Christian Cambillau, Frédéric Carrière

Biochemistry 47:9553-9564 (2008)10.1021/bi8005576

2008
Occurrence of pancreatic lipase-related protein-2 in various species and its relationship with herbivore diet

Josiane de Caro, Cécilia Eydoux, Slim Chérif, Régine Lebrun, youssef Gargouri, Frédéric Carrière, Alain de Caro

Comparative Biochemistry and Physiology. Part B, Biochemistry and Molecular Biology 150:1-9 (2008)10.1016/j.cbpb.2008.01.007

2007

2007
Further biochemical characterization of human pancreatic lipase-related protein 2 expressed in yeast cells

Cécilia Eydoux, Josiane de Caro, Francine Ferrato, Paul Boullanger, Dominique Lafont, René Laugier, Frédéric Carrière, Alain de Caro

Journal of Lipid Research 48:1539-1549 (2007)10.1194/jlr.M600486-JLR200

2006

2006
Human pancreatic lipase-related protein 2: Tissular localization along the digestive tract and quantification in pancreatic juice using a specific ELISA

Cécilia Eydoux, Ahmed Aloulou, Josiane de Caro, Philippe Grandval, René Laugier, Frédéric Carrière, Alain de Caro

Biochimica et Biophysica Acta (BBA) - General Subjects 1760:1497-1504 (2006)10.1016/j.bbagen.2006.06.005