Cécilia Eydoux
Ingénieure de recherche
Responsable opérationnelle Plateforme de Criblage Marseille-Luminy (PCML)
2007-2022 : Ingénieure de recherche (AFMB – UMR7257)
Sujet : Drug-design contre les virus émergents. Production/purification de protéines recombinantes (bactérie). Synthèse d’ARN in vitro. Tests enzymatiques radioactifs et fluorescents sur les polymérases à ARN de différents virus (Dengue, Zika, HCV,Coronavirus…), criblage d’inhibiteurs et étude d’inhibition in vitro. Mise au point de nouvelles techniques (tests fluorescents (HTRF…) ou radioactifs, robotisation, miniaturisation). Gestion de base de données. Encadrement d’étudiants.
2004-2007 : Doctorat (EIPL – UPR9025 Marseille).
Sujet : Etudes biochimiques et physiopathologiques de la lipase pancréatique apparentée de type 2 chez l’homme (HPLRP2). Production/purification de protéines recombinantes (levure). Détermination des propriétés biochimiques, enzymatiques et physiologiques. Etudes immuno-histologiques sur tissus, liquides et biopsies humains. Cristallisation de la HPLRP2. Etudes d’inhibition in vitro.
Publications
2023
AT-752 targets multiple sites and activities on the Dengue virus replication enzyme NS5
Mikael Feracci, Cécilia Eydoux, Véronique Fattorini, Lea Lo Bello, Pierre Gauffre, Barbara Selisko, Priscila Sutto-Ortiz, Ashleigh Shannon, Hongjie Xia, Pei-Yong Shi, Mathieu Noel, Françoise Debart, Jean-Jacques Vasseur, Steve Good, Kai Lin, Adel Moussa, Jean-Pierre Sommadossi, Aurélie Chazot, Karine Alvarez, Jean-Claude Guillemot, Etienne Decroly, François Ferron, Bruno Canard
Antiviral Research 212:105574 (2023)10.1016/j.antiviral.2023.105574
2022
A Versatile Class of 1,4,4-Trisubstituted Piperidines Block Coronavirus Replication In Vitro
Sonia de Castro, Annelies Stevaert, Miguel Maldonado, Adrien Delpal, Julie Vandeput, Benjamin van Loy, Cecilia Eydoux, Jean-Claude Guillemot, Etienne Decroly, Federico Gago, Bruno Canard, Maria-Jose Camarasa, Sonsoles Velázquez, Lieve Naesens
Pharmaceuticals 15:1021 (2022)10.3390/ph15081021
A dual mechanism of action of AT-527 against SARS-CoV-2 polymerase
Ashleigh Shannon, Véronique Fattorini, Bhawna Sama, Barbara Selisko, Mikael Feracci, Camille Falcou, Pierre Gauffre, Priscila El Kazzi, Adrien Delpal, Etienne Decroly, Karine Alvarez, Cécilia Eydoux, Jean-Claude Guillemot, Adel Moussa, Steven S Good, Paolo La Colla, Kai Lin, Jean-Pierre Sommadossi, Yingxiao Zhu, Xiaodong Yan, Hui Shi, Francois Ferron, Bruno Canard
Nature Communications 13 (2022)10.1038/s41467-022-28113-1
Synthesis, Structure–Activity Relationships, and Antiviral Profiling of 1-Heteroaryl-2-Alkoxyphenyl Analogs As Inhibitors of SARS-CoV-2 Replication
Dorothée Bardiot, Laura Vangeel, Mohamed Koukni, Philippe Arzel, Marleen Zwaagstra, Heyrhyoung Lyoo, Patrick Wanningen, Shamshad Ahmad, Linlin Zhang, Xinyuanyuan Sun, Adrien Delpal, Cecilia Eydoux, Jean-Claude Guillemot, Eveline Lescrinier, Hugo Klaassen, Pieter Leyssen, Dirk Jochmans, Karolien Castermans, Rolf Hilgenfeld, Colin Robinson, Etienne Decroly, Bruno Canard, Eric J Snijder, Martijn J Van Hemert, Frank Van van Kuppeveld, Patrick Chaltin, Johan Neyts, Steven De de Jonghe, Arnaud Marchand
Molecules 27:1052 (2022)10.3390/molecules27031052
2021
System-oriented optimization of multi-target 2,6-diaminopurine derivatives: Easily accessible broad-spectrum antivirals active against flaviviruses, influenza virus and SARS-CoV-2
Ilaria Vicenti, Maria Grazia Martina, Adele Boccuto, Marta de Angelis, Giorgia Giavarini, Filippo Dragoni, Serena Marchi, Claudia Maria Trombetta, Emmanuele Crespan, Giovanni Maga, Cecilia Eydoux, Etienne Decroly, Emanuele Montomoli, Lucia Nencioni, Maurizio Zazzi, Marco Radi
European Journal of Medicinal Chemistry 224:113683 (2021)10.1016/j.ejmech.2021.113683
Simeprevir Potently Suppresses SARS-CoV-2 Replication and Synergizes with Remdesivir
Ho Sing Lo, Kenrie Pui Yan Hui, Hei-Ming Lai, Xu He, Khadija Shahed Khan, Simranjeet Kaur, Junzhe Huang, Zhongqi Li, Anthony Chan, Hayley Hei-Yin Cheung, Ka-Chun Ng, John Chi Wang Ho, Yu Wai Chen, Bowen Ma, Peter Man-Hin Cheung, Donghyuk Shin, Kaidao Wang, Meng-Hsuan Lee, Barbara Selisko, Cecilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Kuen-Phon Wu, Po-Huang Liang, Ivan Dikic, Zhong Zuo, Francis Chan, David Hui, Vincent Mok, Kam-Bo Wong, Chris Ka Pun Mok, Ho Ko, Wei Shen Aik, Michael Chi Wai Chan, Wai-Lung Ng
ACS Central Science 7:792-802 (2021)10.1021/acscentsci.0c01186
A fluorescence-based high throughput-screening assay for the SARS-CoV RNA synthesis complex
Cecilia Eydoux, Veronique Fattorini, Ashleigh Shannon, Thi-Tuyet-Nhung Le, Bruno Didier, Bruno Canard, Jean-Claude Guillemot
Journal of Virological Methods 288:114013 (2021)10.1016/j.jviromet.2020.114013
2020
Remdesivir and SARS-CoV-2: Structural requirements at both nsp12 RdRp and nsp14 Exonuclease active-sites
Ashleigh Shannon, Nhung Thi-Tuyet Le, Barbara Selisko, Cecilia Eydoux, Karine Alvarez, Jean-Claude Guillemot, Etienne Decroly, Olve Peersen, Francois Ferron, Bruno Canard
Antiviral Research 178:104793 (2020)10.1016/j.antiviral.2020.104793
Carneic Acids from an Endophytic Phomopsis sp. as Dengue Virus Polymerase Inhibitors
Laure-Anne Peyrat, Véronique Eparvier, Cecilia Eydoux, Jean-Claude Guillemot, Marc Litaudon, Didier Stien
Journal of Natural Products 83:2330-2336 (2020)10.1021/acs.jnatprod.9b01169
2019
Antiviral Compounds from Codiaeum peltatum Targeted by a Multi-informative Molecular Networks Approach
Florent Olivon, Simon Remy, Gwendal Grelier, Cécile Apel, Cecilia Eydoux, Jean-Claude Guillemot, Johan Neyts, Leen Delang, David Touboul, Fanny Roussi, Marc Litaudon
Journal of Natural Products 82:330-340 (2019)10.1021/acs.jnatprod.8b00800
Approved drugs screening against the nsP1 capping enzyme of Venezuelan equine encephalitis virus using an immuno-based assay
Ana Ferreira-Ramos, Changqing Li, Cecilia Eydoux, Jean Marie Contreras, Christophe Morice, Gilles Querat, Alba Gigante, María-Jesús Pérez Pérez, Marie-Louise Jung, Bruno Canard, Jean-Claude Guillemot, Etienne Decroly, Bruno Coutard
Antiviral Research 163:59-69 (2019)10.1016/j.antiviral.2019.01.003
2016
Betulinic Acid, The First Lupane-Type Triterpenoid Isolated from Both a Phomopsis sp. and Its Host Plant Diospyros carbonaria Benoist
Laure-Anne Peyrat, Véronique Eparvier, Cécilia Eydoux, Jean-Claude Guillemot, Marc Litaudon, Didier Stien
Chemistry and Biodiversity 14:e1600171 (2016)10.1002/cbdv.201600171
Protein-Protein Interaction Inhibition (2P21)-Oriented Chemical Library Accelerates Hit Discovery
Sabine Milhas, Brigitt Raux, Stéphane Betzi, Carine Derviaux, Philippe Roche, Audrey Restouin, Marie-Jeanne Basse, Etienne Rebuffet, Adrien Lugari, Marion Badol, Rudra Kashyap, Jean-Claude Lissitzky, Cecilia Eydoux, Veronique Hamon, Marie -Edith Gourde, Sebastien Combes, Pascale Zimmermann, Michel Aurrand-Lions, Thomas Roux, Catherine Rogers, Susanne Muller, Stefan Knapp, Eric Trinquet, Yves Collette, Jean-Claude Guillemot, Xavier Morelli
ACS Chemical Biology 11:2140-2148 (2016)10.1021/acschembio.6b00286
Novel 2-phenyl-5-[(E)-2-(thiophen-2-yl)ethenyl]-1,3,4-oxadiazole and 3-phenyl-5-[(E)-2-(thiophen-2-yl)ethenyl]-1,2,4-oxadiazole derivatives as dengue virus inhibitors targeting NS5 polymerase
Fatiha Benmansour, Cecilia Eydoux, Gilles Querat, Xavier de Lamballerie, Bruno Canard, Karine Alvarez, Jean-Claude Guillemot, Karine Barral
European Journal of Medicinal Chemistry 109:146-156 (2016)10.1016/j.ejmech.2015.12.046
Chemical diversity and antiviral potential in the pantropical Diospyros genus
Laure-Anne Peyrat, Véronique Eparvier, Cécilia Eydoux, Jean-Claude Guillemot, Didier Stien, Marc Litaudon
Fitoterapia (2016)10.1016/j.fitote.2016.04.017
2012
Antiviral chlorinated daphnane diterpenoid orthoesters from the bark and wood of Trigonostemon cherrieri.
Pierre-Marie Allard, Pieter Leyssen, Marie-Thérèse Martin, Mélanie Bourjot, Vincent Dumontet, Cécilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Cyril Poullain, Françoise Guéritte, Marc Litaudon
Phytochemistry 84:160-8 (2012)10.1016/j.phytochem.2012.07.023
Chemical constituents of Anacolosa pervilleana and their antiviral activities.
Mélanie Bourjot, Pieter Leyssen, Cécilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Philippe Rasoanaivo, Françoise Guéritte, Marc Litaudon
Fitoterapia 83:1076-1080 (2012)10.1016/j.fitote.2012.05.004
Flacourtosides A-F, Phenolic Glycosides Isolated from Flacourtia ramontchi.
Mélanie Bourjot, Pieter Leyssen, Cécilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Philippe Rasoanaivo, Françoise Guéritte, Marc Litaudon
Journal of Natural Products 75:752-8 (2012)10.1021/np300059n
2011
Alkylated flavanones from the bark of Cryptocarya chartacea as dengue virus NS5 polymerase inhibitors.
Pierre-Marie Allard, Elise Tran Huu Dau, Cécilia Eydoux, Jean-Claude Guillemot, Vincent Dumontet, Cyril Poullain, Bruno Canard, Françoise Guéritte, Marc Litaudon
Journal of Natural Products 74:2446-53 (2011)10.1021/np200715v
2009
Identification of allosteric inhibitors blocking the hepatitis C virus polymerase NS5B in the RNA synthesis initiation step.
Stéphane Betzi, Cécilia Eydoux, Cécile Bussetta, Marilyne Blemont, Pieter Leyssen, Claire Debarnot, Mohamed Ben-Rahou, Jacques Haiech, Marcel Hibert, Françoise Gueritte, David S. Grierson, Jean-Louis Romette, Jean-Claude Guillemot, Johan Neyts, Karine Alvarez, Xavier Morelli, Hélène Dutartre, Bruno Canard
Antiviral Research 84:48-59 (2009)10.1016/j.antiviral.2009.06.009
2008
Structure of Human Pancreatic Lipase-Related Protein 2 with the Lid in an Open Conformation ,
Cécilia Eydoux, Silvia Spinelli, Tara Davis, John Walker, Alma Seitova, Sirano Dhe-Paganon, Alain de Caro, Christian Cambillau, Frédéric Carrière
Biochemistry 47:9553-9564 (2008)10.1021/bi8005576
Occurrence of pancreatic lipase-related protein-2 in various species and its relationship with herbivore diet
Josiane de Caro, Cécilia Eydoux, Slim Chérif, Régine Lebrun, Youssef Gargouri, Frédéric Carrière, Alain de Caro
Comparative Biochemistry and Physiology. Part B, Biochemistry and Molecular Biology 150:1-9 (2008)10.1016/j.cbpb.2008.01.007
2007
Further biochemical characterization of human pancreatic lipase-related protein 2 expressed in yeast cells
Cécilia Eydoux, Josiane de Caro, Francine Ferrato, Paul Boullanger, Dominique Lafont, René Laugier, Frédéric Carrière, Alain de Caro
Journal of Lipid Research 48:1539-1549 (2007)10.1194/jlr.M600486-JLR200
2006
Human pancreatic lipase-related protein 2: Tissular localization along the digestive tract and quantification in pancreatic juice using a specific ELISA
Cécilia Eydoux, Ahmed Aloulou, Josiane de Caro, Philippe Grandval, René Laugier, Frédéric Carrière, Alain de Caro
Biochimica et Biophysica Acta (BBA) - General Subjects 1760:1497-1504 (2006)10.1016/j.bbagen.2006.06.005
