Invitée équipe B. Canard: Caroline Demeret, Institut Pasteur -« Comparative host/pathogens “contactomics” dedicated to infectious diseases »

Résumé

The ability of intracellular pathogens to take control the cell machinery for their own replication cycle and for evading intracellular immunity governs their pathogenic power, virulence, and ultimately infection outcome. Unbiased, system-level host/pathogens protein-protein (PPIs) interaction mapping reveals pathogens’ takeover of the cell proteome, which are initiated by the physical contacts that pathogens proteins establish with the host proteins. We are exploring such direct host/pathogens PPIs (“contactome”) using mammalian cell-based split-luciferase assays and applying our strategies to various human viruses. This supervised and systematic interactomics pipeline (“contactomics”) offers a compelling complement of unsupervised proteome wide host/pathogens mappings and of structure-based characterization of protein complexes. I will provide an overview of the interactomics pipelines we are developing, particularly focusing on their application to SARS-CoV-2.

Caroline Demeret
Interactomics RNA and Immunity Virology department
Institut Pasteur, Paris
Caroline.demeret@pasteur.fr