Florence Vincent
Chargée de recherche
Responsable du thème Glycobiologie bactérienne de l’équipe
Correspondante communication
Curriculum Vitae
2001 Doctorat en Biologie Structurale à l’Université d’Aix-Marseille (directeur : Dr Christian Cambillau),
2002-2004 Post-doctorat au York Structural Biology Laboratory, Dept. Chemistry (directeur: Dr Gideon Davies), Royaume-Uni.
2017 Habilitation à Diriger des Recherches, Université Aix-Marseille
Glycobiologie bactérienne, pathogénicité et virulence
Mes recherches sont centrées sur la structure et la fonction d’enzymes impliquées dans la lyse de la paroi bactérienne et le développement de biofilm par les bactéries pathogènes…
Publications
2022
Insights into the atypical autokinase activity of the Pseudomonas aeruginosa GacS histidine kinase and its interaction with RetS
Firas Fadel, Violla Bassim, Vanessa Francis, Steven Porter, Thomas Botzanowski, Pierre Legrand, Maria Maté Perez, Yves Bourne, Sarah Cianférani, Florence Vincent
Structure 22:S0969-2126 (2022)10.1016/j.str.2022.06.002
Molecular basis for substrate recognition and septum cleavage by AtlA, the major N-acetylglucosaminidase of Enterococcus faecalis
Véronique Roig-Zamboni, Sarah Barelier, Robert Dixon, Nicola Galley, Amani Ghanem, Quoc Phong Nguyen, Héloize Cahuzac, Bartłomiej Salamaga, Peter Davis, Yves Bourne, Stéphane Mesnage, Florence Vincent
Journal of Biological Chemistry 298:101915 (2022)10.1016/j.jbc.2022.101915
2020
Biochemical characterization of a glycosyltransferase Gtf3 from Mycobacterium smegmatis: a case study of improved protein solubilization
Mahfoud Bakli, Loukmane Karim, Nassima Mokhtari-Soulimane, Hafida Merzouk, Florence Vincent
3 Biotech 10 (2020)10.1007/s13205-020-02431-x
2018
Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes
Wade Abbott, Orly Alber, Ed Bayer, Jean-Guy Berrin, Alisdair Boraston, Harry Brumer, Ryszard Brzezinski, Anthony Clarke, Beatrice Cobucci-Ponzano, Darrell Cockburn, Pedro Coutinho, Mirjam Czjzek, Bareket Dassa, Gideon John Davies, Vincent Eijsink, Jens Eklof, Alfons Felice, Elizabeth Ficko-Blean, Geoff Pincher, Thierry Fontaine, Zui Fujimoto, Kiyotaka Fujita, Shinya Fushinobu, Harry Gilbert, Tracey Gloster, Ethan Goddard-Borger, Ian Greig, Jan-Hendrik Hehemann, Glyn Hemsworth, Bernard Henrissat, Masafumi Hidaka, Ramon Hurtado-Guerrero, Kiyohiko Igarashi, Takuya Ishida, Stefan Janecek, Seino Jongkees, Nathalie Juge, Satoshi Kaneko, Takane Katayama, Motomitsu Kitaoka, Naotake Konno, Daniel Kracher, Anna Kulminskaya, Alicia Lammerts van Bueren, Sine Larsen, Junho Lee, Markus Linder, Leila Loleggio, Roland Ludwig, Ana Luis, Mirko Maksimainen, Brian Mark, Richard Mclean, Gurvan Michel, Cédric Montanier, Marco Moracci, Haruhide Mori, Hiroyuki Nakai, Wim Nerinckx, Takayuki Ohnuma, Richard Pickersgill, Kathleen Piens, Tirso Pons, Etienne Rebuffet, Peter Reilly, Magali Remaud Simeon, Brian Rempel, Kyle Robinson, David Rose, Juha Rouvinen, Wataru Saburi, Yuichi Sakamoto, Mats Sandgren, Fathima Shaikh, Yuval Shoham, Franz St John, Jerry Stahlberg, Michael Suits, Gerlind Sulzenbacher, Tomomi Sumida, Ryuichiro Suzuki, Birte Svensson, Toki Taira, Ed Taylor, Takashi Tonozuka, Breeanna Urbanowicz, Gustav Vaaje-Kolstad, Wim van den Ende, Annabelle Varrot, Maxime Versluys, Florence Vincent, David Vocadlo, Warren Wakarchuk, Tom Wennekes, Rohan Williams, Spencer Williams, David Wilson, Stephen Withers, Katsuro Yaoi, Vivian Yip, Ran Zhang
Glycobiology 28:3-8 (2018)10.1093/glycob/cwx089
2017
Structural and functional insights into the periplasmic detector domain of the GacS histidine kinase controlling biofilm formation in Pseudomonas aeruginosa
Ahmad Ali-Ahmad, Firas Fadel, Corinne Sebban-Kreuzer, Moly Ba, Gauthier Dangla Pélissier, Olivier Bornet, Françoise Guerlesquin, Yves Bourne, Christophe Bordi, Florence Vincent
Scientific Reports 7:11262 (2017)10.1038/s41598-017-11361-3
Structural insights into a family 39 glycoside hydrolase from the gut symbiont Bacteroides cellulosilyticus WH2
Ahmad Ali-Ahmad, Marie-Line Garron, Véronique Roig-Zamboni, Nicolas Lenfant, Didier Nurizzo, Bernard Henrissat, Jean-Guy Berrin, Yves Bourne, Florence Vincent
Journal of Structural Biology 197:227-235 (2017)10.1016/j.jsb.2016.11.004
NMR assignments of the GacS histidine-kinase periplasmic detection domain from Pseudomonas aeruginosa PAO1
Ahmad Ali-Ahmad, Olivier Bornet, Firas Fadel, Yves Bourne, Florence Vincent, Christophe Bordi, Françoise Guerlesquin, Corinne Sebban-Kreuzer
Biomolecular NMR Assignments 11:25-28 (2017)10.1007/s12104-016-9714-7
2015
Identification of the nucleophile catalytic residue of GH51 alpha-L-arabinofuranosidase from Pleurotus ostreatus
Antonella Amore, Alfonso Iadonisi, Florence Vincent, Vincenza Faraco
AMB Express 5 (2015)10.1186/s13568-015-0164-x
Structural and biochemical characterization of the -N-acetylglucosaminidase from Thermotoga maritima: Toward rationalization of mechanistic knowledge in the GH73 family
A. Lipski, M. Herve, V. Lombard, D. Nurizzo, D. Mengin-Lecreulx, Y. Bourne, Florence Vincent
Glycobiology 25:319-330 (2015)10.1093/glycob/cwu113
2014
Development of an improved variant of GH51 α-l-arabinofuranosidase from Pleurotus ostreatus by directed evolution
Simona Giacobbe, Florence Vincent, Vincenza Faraco
New Biotechnology 31:230-236 (2014)10.1016/j.nbt.2014.02.004
Structural and biochemical characterization of the β-N-acetylglucosaminidase from Thermotoga maritima: toward rationalization of mechanistic knowledge in the GH73 family
Alexandra Lipski, Mireille Hervé, Vincent Lombard, Didier Nurizzo, Dominique Mengin-Lecreulx, Yves Bourne, Florence Vincent
Glycobiology 25:319-330 (2014)10.1093/glycob/cwu113
Structural and Functional Characterization of the Clostridium perfringens N-Acetylmannosamine-6-phosphate 2-Epimerase Essential for the Sialic Acid Salvage Pathway
Marie-Cécile Pélissier, Corinne Sebban-Kreuzer, Françoise Guerlesquin, James A Brannigan, Yves Bourne, Florence Vincent
Journal of Biological Chemistry 289:35215-35224 (2014)10.1074/jbc.m114.604272
2010
Crystal structures of a family 8 polysaccharide lyase reveal open and highly occluded substrate-binding cleft conformations
Zainab H Elmabrouk, Florence Vincent, Meng Zhang, Nicola L Smith, Johan P Turkenburg, Simon J Charnock, Gary W Black, Edward J Taylor
Proteins - Structure, Function and Bioinformatics 79:965-974 (2010)10.1002/prot.22938
Structure of a polyisoprenoid binding domain from Saccharophagus degradans implicated in plant cell wall breakdown
Florence Vincent, David Dal Molin, Ronald Weiner, Yves Bourne, Bernard Henrissat
FEBS Letters 584:1577-1584 (2010)10.1016/j.febslet.2010.03.015
2009
Distinct oligomeric forms of the Pseudomonas aeruginosa RetS sensor domain modulate accessibility to the ligand binding site
Florence Vincent, Adam Round, Aline Reynaud, Christophe Bordi, Alain Filloux, Yves Bourne
Environmental Microbiology 12:1775-1786 (2009)10.1111/j.1462-2920.2010.02264.x
2006
Structure and Activity of Two Metal Ion-dependent Acetylxylan Esterases Involved in Plant Cell Wall Degradation Reveals a Close Similarity to Peptidoglycan Deacetylases
Edward J. Taylor, Tracey M. Gloster, Johan P. Turkenburg, Florence Vincent, A. Marek Brzozowski, Claude Dupont, François Shareck, Maria S.J. Centeno, José A.M. Prates, Vladimír Puchart, Luís M.A. Ferreira, Carlos M.G.A. Fontes, Peter Biely, Gideon J. Davies
Journal of Biological Chemistry 281:10968-10975 (2006)10.1074/jbc.M513066200
2005
Structure and Kinetics of a Monomeric Glucosamine 6-Phosphate Deaminase
Florence Vincent, Gideon J Davies, James A Brannigan
Journal of Biological Chemistry 280:19649-19655 (2005)10.1074/jbc.m502131200
2004
The Use of Forced Protein Evolution to Investigate and Improve Stability of Family 10 Xylanases
Simon Andrews, Edward Taylor, Gavin Pell, Florence Vincent, Valérie M.-A. Ducros, Gideon Davies, Jeremy Lakey, Harry Gilbert
Journal of Biological Chemistry 279:54369-54379 (2004)10.1074/jbc.M409044200
Common Inhibition of Both β-Glucosidases and β-Mannosidases by Isofagomine Lactam Reflects Different Conformational Itineraries for Pyranoside Hydrolysis
Florence Vincent, Tracey Gloster, James Macdonald, Carl Morland, Robert Stick, Fernando Dias, José Prates, Carlos Fontes, Harry Gilbert, Gideon Davies
ChemBioChem 5:1596-1599 (2004)10.1002/cbic.200400169
Crystal structures of bovine odorant-binding protein in complex with odorant molecules
Florence Vincent, Roberto Ramoni, Silvia Spinelli, Stefano Grolli, Mariella Tegoni, Christian Cambillau
European Journal of Biochemistry 271:3832-3842 (2004)10.1111/j.1432-1033.2004.04315.x
Insights into the Molecular Determinants of Substrate Specificity in Glycoside Hydrolase Family 5 Revealed by the Crystal Structure and Kinetics of Cellvibrio mixtus Mannosidase 5A
Fernando M V Dias, Florence Vincent, Gavin Pell, José a M Prates, Maria S J Centeno, Louise E Tailford, Luís M A Ferreira, Carlos M G A Fontes, Gideon J Davies, Harry J Gilbert
Journal of Biological Chemistry 279:25517-25526 (2004)10.1074/jbc.m401647200
2003
The Three-dimensional Structure of the N-Acetylglucosamine-6-phosphate Deacetylase, NagA, from Bacillus subtilis
Florence Vincent, David Yates, Elspeth Garman, Gideon J Davies, James A Brannigan
Journal of Biological Chemistry 279:2809-2816 (2003)10.1074/jbc.m310165200
Multifunctional Xylooligosaccharide/Cephalosporin C Deacetylase Revealed by the Hexameric Structure of the Bacillus subtilis Enzyme at 1.9Å Resolution
Florence Vincent, Simon Charnock, Koen H.G. Verschueren, Johan Turkenburg, David Scott, Wendy Offen, Shirley Roberts, Gavin Pell, Harry Gilbert, Gideon Davies, James Brannigan
Journal of Molecular Biology 330:593-606 (2003)10.1016/s0022-2836(03)00632-6
2002
Boar salivary lipocalin
Silvia Spinelli, Florence Vincent, Paolo Pelosi, Mariella Tegoni, Christian Cambillau
European Journal of Biochemistry 269:2449-2456 (2002)10.1046/j.1432-1033.2002.02901.x
Control of domain swapping in bovine odorant-binding protein
Roberto Ramoni, Florence Vincent, Alison E Ashcroft, Paolo Accornero, Stefano Grolli, Christel Valencia, Mariella Tegoni, Christian Cambillau
Biochemical Journal (2002)
2001
Crystal structure of aphrodisin, a sex pheromone from female hamster11Edited by R Huber
Florence Vincent, Dietrich Löbel, Kieron Brown, Silvia Spinelli, Phillip Grote, Heinz Breer, Christian Cambillau, Mariella Tegoni
Journal of Molecular Biology 305:459-469 (2001)10.1006/jmbi.2000.4241
The insect attractant 1-Octen-3-o1 is the natural ligand of bovine odorant-binding protein
Roberto Ramoni, Florence Vincent, Stefano Grolli, Virna Conti, Christian Malosse, Francois Boyer, Patricia Nagnan-Le Meillour, Silvia Spinelli, Christian Cambillau, Mariella Tegoni
Journal of Biological Chemistry 276:7150-7155 (2001)10.1074/jbc.M010368200
2000
Mammalian odorant binding proteins
Mariella Tegoni, Paolo Pelosi, Florence Vincent, Silvia Spinelli, Valérie Campanacci, Stefano Grolli, Roberto Ramoni, Christian Cambillau
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 1482:229-240 (2000)10.1016/s0167-4838(00)00167-9
Complexes of porcine odorant binding protein with odorant molecules belonging to different chemical classes
Florence Vincent, Silvia Spinelli, Roberto Ramoni, Stefano Grolli, Paolo Pelosi, Christian Cambillau, Mariella Tegoni
Journal of Molecular Biology 300:127-139 (2000)10.1006/jmbi.2000.3820
1999
RNA recognition by transcriptional antiterminators of the BglG/SacY family: functional and structural comparison of the CAT domain from SacY and LicT
Nathalie Declerck, Florence Vincent, François Hoh, Stéphane Aymerich, Herman van Tilbeurgh
Journal of Molecular Biology 294:389-402 (1999)10.1006/jmbi.1999.3256
