Yves Bourne
Directeur de recherche
Yves Bourne est directeur de recherche au CNRS. Aprés un doctorat en 1990 à l’Université Aix-Marseille III intitulé “Etude cristallographique de l’interaction entre une lectine végétale et divers saccharides” sous la direction de C Cambillau, il a intégré le laboratoire LCCMB (ensuite renommé laboratoire AFMB) en 1991 en tant que chargé de recherche CNRS. En 1994, il rejoint l’équipe du Pr John A. Tainer au Scripps Research Institute à La Jolla (CA, USA) pour travailler sur des protéines régulatrices du cycle cellulaire lors d’un séjour sabbatique de 2 ans. Depuis 2003, il est responsabble, et depuis 2012 co-responsable avec Pascale Marchot, de l’équipe « Glycobiologie et Neurobiologie Structurales » du laboratoire AFMB. Depuis 2008, il est directeur du laboratoire AFMB (CNRS / Aix-Marseille Université) après en avoir été le directeur-adjoint en 2007.
Il s’intéresse à élucider l’architecture, principalement par cristallographie aux rayons X, et le mode d’action de protéines ou d’enzymes agissant sur les glycanes mais aussi de protéines synaptiques et des complexes qu’elles peuvent former avec d’autres partenaires protéiques ou glycoprotéines. Les résultats de ces travaux ont un fort intérêt biomédical et permettent d’appréhender les bases moléculaires de certains désordres de la glycosylation des protéines, certaines infections pulmonaires ou des troubles autistiques.
Il est co-auteur d’une centaine d’articles dans des revues à comité de lecture et a contribué à plus d’une centaine de structures déposées dans la “Protein Data Bank”.
Publications
2024
The Cyclic Imine Core Common to the Marine Macrocyclic Toxins Is Sufficient to Dictate Nicotinic Acetylcholine Receptor Antagonism
Yves Bourne, Gerlind Sulzenbacher, Laurent Chabaud, Rómulo Aráoz, Zoran Radić, Sandrine Conrod, Palmer Taylor, Catherine Guillou, Jordi Molgó, Pascale Marchot
Marine drugs 22:149 (2024)10.3390/md22040149
2022
Direct capture, inhibition and crystal structure of HsaD (Rv3569c) from M. tuberculosis
Sarah Barelier, Romain Avellan, Giri Raj Gnawali, Patrick Fourquet, Véronique Roig-Zamboni, Isabelle Poncin, Vanessa Point, Yves Bourne, Stéphane Audebert, Luc Camoin, Christopher Spilling, Stéphane Canaan, Jean‐françois Cavalier, Gerlind Sulzenbacher
FEBS Journal (2022)10.1111/febs.16645
Insights into the atypical autokinase activity of the Pseudomonas aeruginosa GacS histidine kinase and its interaction with RetS
Firas Fadel, Violla Bassim, Vanessa Francis, Steven Porter, Thomas Botzanowski, Pierre Legrand, Maria Maté Perez, Yves Bourne, Sarah Cianférani, Florence Vincent
Structure 22:S0969-2126 (2022)10.1016/j.str.2022.06.002
Molecular basis for substrate recognition and septum cleavage by AtlA, the major N-acetylglucosaminidase of Enterococcus faecalis
Véronique Roig-Zamboni, Sarah Barelier, Robert Dixon, Nicola Galley, Amani Ghanem, Quoc Phong Nguyen, Héloize Cahuzac, Bartłomiej Salamaga, Peter Davis, Yves Bourne, Stéphane Mesnage, Florence Vincent
Journal of Biological Chemistry 298:101915 (2022)10.1016/j.jbc.2022.101915
2021
Comparative mapping of selected structural determinants on the extracellular domains of cholinesterase-like cell-adhesion molecules.
Davide Comoletti, Laura Trobiani, Arnaud Chatonnet, Yves Bourne, Pascale Marchot
Neuropharmacology 184:108381 (2021)10.1016/j.neuropharm.2020.108381
2020
The neuroligins and the synaptic pathway in Autism Spectrum Disorder.
Laura Trobiani, Maria Meringolo, Tamara Diamanti, Yves Bourne, Pascale Marchot, Giuseppina Martella, Luciana Dini, Antonio Pisani, Antonella de Jaco, Paola Bonsi
Neuroscience and Biobehavioral Reviews 119:37-51 (2020)10.1016/j.neubiorev.2020.09.017
The Ig-like domain of Punctin/MADD-4 is the primary determinant for interaction with the ectodomain of neuroligin NLG-1
Semeli Platsaki, Xin Zhou, Bérangère Pinan-Lucarré, Vincent Delauzun, Haijun Tu, Pascal Mansuelle, Patrick Fourquet, Yves Bourne, Jean-Louis Bessereau, Pascale Marchot
Journal of Biological Chemistry 16267-16279. (2020)10.1074/jbc.RA120.014591
2019
Substrate binding mode and catalytic mechanism of human heparan sulfate d-glucuronyl C5 epimerase
Claire Debarnot, Yoan Monneau, Veronique Roig-Zamboni, Vincent Delauzun, Christine Le Narvor, Emeline Richard, Jérôme Hénault, Adeline Goulet, Firas Fadel, Romain R Vivès, Bernard Priem, David Bonnaffé, Hugues Lortat-Jacob, Yves Bourne
Proceedings of the National Academy of Sciences of the United States of America 116:6760-6765 (2019)10.1073/pnas.1818333116
Overview of the Structure–Function Relationships of Mannose-Specific Lectins from Plants, Algae and Fungi
Annick Barre, Yves Bourne, Els J. M. van Damme, Pierre Rougé
International Journal of Molecular Sciences 20:254 (2019)10.3390/ijms20020254
An evolutionary perspective on the first disulfide bond in members of the cholinesterase-carboxylesterase (COesterase) family: Possible outcomes for cholinesterase expression in prokaryotes
Arnaud Chatonnet, Xavier Brazzolotto, Thierry Hotelier, Nicolas Lenfant, Pascale Marchot, Yves Bourne
Chemico-Biological Interactions 308:179-184 (2019)10.1016/j.cbi.2019.05.016
2018
Genetic and enzymatic characterization of 3-O-sulfotransferase SNPs associated with Plasmodium falciparum parasitaemia
Ngoc Thy Nguyen, Romain R Vivès, Magali Iché-Torres, Vincent Delauzun, Els Saesen, Veronique Roig-Zamboni, Hugues Lortat-Jacob, Pascal Rihet, Yves Bourne
Glycobiology 28:534-541 (2018)10.1093/glycob/cwy038
Hot Spots for Protein Partnerships at the Surface of Cholinesterases and Related α/β Hydrolase Fold Proteins or Domains—A Structural Perspective
Yves Bourne, Pascale Marchot
Molecules 23 (2018)10.3390/molecules23010035
Glycosylate and move! The glycosyltransferase Maf is involved in bacterial flagella formation
Gerlind Sulzenbacher, Veronique Roig-Zamboni, Régine Lebrun, Yann Guérardel, Dorothée Murat, Pascal Mansuelle, Nao Yamakawa, Xin-Xin Qian, Renaud Vincentelli, Yves Bourne, Long-Fei Wu, François Alberto
Environmental Microbiology 20:228-240 (2018)10.1111/1462-2920.13975
2017
Structural and functional insights into the periplasmic detector domain of the GacS histidine kinase controlling biofilm formation in Pseudomonas aeruginosa
Ahmad Ali-Ahmad, Firas Fadel, Corinne Sebban-Kreuzer, Moly Ba, Gauthier Dangla Pélissier, Olivier Bornet, Françoise Guerlesquin, Yves Bourne, Christophe Bordi, Florence Vincent
Scientific Reports 7:11262 (2017)10.1038/s41598-017-11361-3
Structure of human lysosomal acid α-glucosidase-a guide for the treatment of Pompe disease
Véronique Roig-Zamboni, Beatrice Cobucci-Ponzano, Roberta Iacono, Maria Carmina Ferrara, Stanley Germany, Yves Bourne, Giancarlo Parenti, Marco Moracci, Gerlind Sulzenbacher
Nature Communications (2017)10.1038/s41467-017-01263-3
Cyclic imine toxins from dinoflagellates: a growing family of potent antagonists of the nicotinic acetylcholine receptors
Jordi Molgó, Pascale Marchot, Rómulo Aráoz, Evelyne Benoit, Bogdan I Iorga, Armen Zakarian, Palmer Taylor, Yves Bourne, Denis Servent
Journal of Neurochemistry 142:41-51 (2017)10.1111/jnc.13995
Structural insights into a family 39 glycoside hydrolase from the gut symbiont Bacteroides cellulosilyticus WH2
Ahmad Ali-Ahmad, Marie-Line Garron, Véronique Roig-Zamboni, Nicolas Lenfant, Didier Nurizzo, Bernard Henrissat, Jean-Guy Berrin, Yves Bourne, Florence Vincent
Journal of Structural Biology 197:227-235 (2017)10.1016/j.jsb.2016.11.004
Structure of human lysosomal acid α-glucosidase–a guide for the treatment of Pompe disease
Veronique Roig-Zamboni, Beatrice Cobucci-Ponzano, Roberta Iacono, Maria Carmina Ferrara, Stanley Germany, Yves Bourne, Giancarlo Parenti, Marco Moracci, Gerlind Sulzenbacher
Nature Communications 8:1111 (2017)10.1038/s41467-017-01263-3
NMR assignments of the GacS histidine-kinase periplasmic detection domain from Pseudomonas aeruginosa PAO1
Ahmad Ali-Ahmad, Olivier Bornet, Firas Fadel, Yves Bourne, Florence Vincent, Christophe Bordi, Françoise Guerlesquin, Corinne Sebban-Kreuzer
Biomolecular NMR Assignments 11:25-28 (2017)10.1007/s12104-016-9714-7
2016
Relationships of human alpha/beta hydrolase fold proteins and other organophosphate-interacting proteins
Nicolas Lenfant, Yves Bourne, Pascale Marchot, Arnaud Chatonnet
Chemico-Biological Interactions 259:343-351 (2016)10.1016/j.cbi.2016.04.027
Steric and Dynamic Parameters Influencing In Situ Cycloadditions to Form Triazole Inhibitors with Crystalline Acetylcholinesterase
Yves Bourne, K. Barry Sharpless, Palmer Taylor, Pascale Marchot
Journal of the American Chemical Society 138:1611-1621 (2016)10.1021/jacs.5b11384
2015
Marine Macrocyclic Imines, Pinnatoxins A and G: Structural Determinants and Functional Properties to Distinguish Neuronal α7 from Muscle α12βγδ nAChRs.
Yves Bourne, Gerlind Sulzenbacher, Zoran Radić, Rómulo Aráoz, Morgane Reynaud, Evelyne Benoit, Armen Zakarian, Denis Servent, Jordi Molgó, Palmer Taylor, Pascale Marchot
Structure (London, England : 1993) 23:1106-15 (2015)10.1016/j.str.2015.04.009
Structural basis for carbohydrate binding properties of a plant chitinase-like agglutinin with conserved catalytic machinery
Gerlind Sulzenbacher, Veronique Roig-Zamboni, Willy J. Peumans, Bernard Henrissat, Els J. M. Van Damme, Yves Bourne
Journal of Structural Biology 190:115-121 (2015)10.1016/j.jsb.2015.01.013
Structural and biochemical characterization of the -N-acetylglucosaminidase from Thermotoga maritima: Toward rationalization of mechanistic knowledge in the GH73 family
A. Lipski, M. Herve, V. Lombard, D. Nurizzo, D. Mengin-Lecreulx, Y. Bourne, Florence Vincent
Glycobiology 25:319-330 (2015)10.1093/glycob/cwu113
Crystal Structure of Snake Venom Acetylcholinesterase in Complex with Inhibitory Antibody Fragment Fab410 Bound at the Peripheral Site EVIDENCE FOR OPEN AND CLOSED STATES OF A BACK DOOR CHANNEL
Yves Bourne, Ludovic Renault, Pascale Marchot
Journal of Biological Chemistry 290:1522-1535 (2015)10.1074/jbc.M114.603902
2014
The neuroligins and their neurexin ligands: from structure to function at the synapse
Yves Bourne, Pascale Marchot
Journal of Molecular Neuroscience 53:387-396 (2014)10.1007/s12031-014-0234-6
Structural and biochemical characterization of the β-N-acetylglucosaminidase from Thermotoga maritima: toward rationalization of mechanistic knowledge in the GH73 family
Alexandra Lipski, Mireille Hervé, Vincent Lombard, Didier Nurizzo, Dominique Mengin-Lecreulx, Yves Bourne, Florence Vincent
Glycobiology 25:319-330 (2014)10.1093/glycob/cwu113
Structural and Functional Characterization of the Clostridium perfringens N-Acetylmannosamine-6-phosphate 2-Epimerase Essential for the Sialic Acid Salvage Pathway
Marie-Cécile Pélissier, Corinne Sebban-Kreuzer, Françoise Guerlesquin, James A Brannigan, Yves Bourne, Florence Vincent
Journal of Biological Chemistry 289:35215-35224 (2014)10.1074/jbc.m114.604272
Tracking the origin and divergence of cholinesterases and neuroligins: the evolution of synaptic proteins
Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot, Arnaud Chatonnet
Journal of Molecular Neuroscience 53:362-369 (2014)10.1007/s12031-013-0194-2
2013
Molecular Characterization of Monoclonal Antibodies that Inhibit Acetylcholinesterase by Targeting the Peripheral Site and Backdoor Region
Yves Bourne, Ludovic Renault, Sosthène Essono, Grégoire Mondielli, Patricia Lamourette, Didier Boquet, Jacques Grassi, Pascale Marchot
PLoS ONE 8:e77226 (2013)10.1371/journal.pone.0077226
ESTHER, the database of the α/β-hydrolase fold superfamily of proteins: tools to explore diversity of functions
Nicolas Lenfant, Thierry Hotelier, Eric Velluet, Yves Bourne, Pascale Marchot, Arnaud Chatonnet
Nucleic Acids Research 41:423-429 (2013)10.1093/nar/gks1154
Proteins with an alpha/beta hydrolase fold: Relationships between subfamilies in an ever-growing superfamily.
Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot, Arnaud Chatonnet
Chemico-Biological Interactions 203:266-268 (2013)10.1016/j.cbi.2012.09.003
2012
The Structure of Human DNase I Bound to Magnesium and Phosphate Ions Points to a Catalytic Mechanism Common to Members of the DNase I-like Superfamily
Goetz Parsiegla, Christophe Noguere, Lydia Santell, Robert Lazarus, Yves Bourne
Biochemistry 51:10250-10258 (2012)10.1021/bi300873f
Structural Insights into Antibody Sequestering and Neutralizing of Na+ Channel α-Type Modulator from Old World Scorpion Venom.
Igor P Fabrichny, Grégoire Mondielli, Sandrine Conrod, Marie-France Martin-Eauclaire, Yves Bourne, Pascale Marchot
Journal of Biological Chemistry 287:14136-48 (2012)10.1074/jbc.M111.315382
2010
Conformational remodeling of femtomolar inhibitor-acetylcholinesterase complexes in the crystalline state.
Yves Bourne, Zoran Radić, Palmer Taylor, Pascale Marchot
Journal of the American Chemical Society 132:18292-300 (2010)10.1021/ja106820e
Structural insights into the exquisite selectivity of neurexin/neuroligin synaptic interactions.
Philippe Leone, Davide Comoletti, Géraldine Ferracci, Sandrine Conrod, Simon U Garcia, Palmer Taylor, Yves Bourne, Pascale Marchot
EMBO Journal 29:2461-71 (2010)10.1038/emboj.2010.123
Structure of a polyisoprenoid binding domain from Saccharophagus degradans implicated in plant cell wall breakdown
Florence Vincent, David Dal Molin, Ronald Weiner, Yves Bourne, Bernard Henrissat
FEBS Letters 584:1577-1584 (2010)10.1016/j.febslet.2010.03.015
Structural determinants in phycotoxins and AChBP conferring high affinity binding and nicotinic AChR antagonism.
Yves Bourne, Zoran Radic, Rómulo Aráoz, Todd T Talley, Evelyne Benoit, Denis Servent, Palmer Taylor, Jordi Molgó, Pascale Marchot
Proceedings of the National Academy of Sciences of the United States of America 107:6076-81 (2010)10.1073/pnas.0912372107
Structure-function relationships of the alpha/beta-hydrolase fold domain of neuroligin: A comparison with acetylcholinesterase.
Philippe Leone, Davide Comoletti, Palmer Taylor, Yves Bourne, Pascale Marchot
Chemico-Biological Interactions epub ahead of print (2010)10.1016/j.cbi.2010.01.030
Crystal Structure of the GalNAc/Gal-Specific Agglutinin from the Phytopathogenic Ascomycete Sclerotinia sclerotiorum Reveals Novel Adaptation of a β-Trefoil Domain
Gerlind Sulzenbacher, Véronique Roig-Zamboni, Willy J Peumans, Pierre Rougé, Els J M van Damme, Yves Bourne
Journal of Molecular Biology 400:715-723 (2010)10.1016/j.jmb.2010.05.038
A novel α-N-acetylgalactosaminidase family with an NAD+-dependent catalytic mechanism suitable for enzymatic removal of blood group A antigens
Gerlind Sulzenbacher, Qiyong Peter Liu, Eric P Bennett, Steven B Levery, Yves Bourne, Guillaume Ponchel, Henrik Clausen, Bernard Henrissat
Biocatalysis and Biotransformation 28:22-32 (2010)10.3109/10242420903424259
2009
Interaction between the SifA Virulence Factor and Its Host Target SKIP Is Essential for Salmonella Pathogenesis
Lautaro Diacovich, Audrey Dumont, Daniel Lafitte, Elodie Soprano, Aude-Agnès Guilhon, Christophe Bignon, Jean-Pierre Gorvel, Yves Bourne, Stéphane Méresse
Journal of Biological Chemistry 284:33151-33160 (2009)10.1074/jbc.m109.034975
Interaction between the sifa virulence factor and its host target skip is essential for salmonella pathogenesis.
Lautaro Diacovich, Audrey Dumont, Daniel Lafitte, Elodie Soprano, Aude-Agnes Guilhon, Christophe Bignon, Jean-Pierre Gorvel, Yves Bourne, Stephane Meresse
Journal of Biological Chemistry epub ahead of print (2009)10.1074/jbc.M109.034975
Structural determinants for interaction of partial agonists with acetylcholine binding protein and neuronal α7 nicotinic acetylcholine receptor
Ryan Hibbs, Gerlind Sulzenbacher, Jianxin Shi, Todd Talley, Sandrine Conrod, William Kem, Palmer Taylor, Pascale Marchot, Yves Bourne
EMBO Journal 28:3040-3051 (2009)10.1038/emboj.2009.227
Distinct oligomeric forms of the Pseudomonas aeruginosa RetS sensor domain modulate accessibility to the ligand binding site
Florence Vincent, Adam Round, Aline Reynaud, Christophe Bordi, Alain Filloux, Yves Bourne
Environmental Microbiology 12:1775-1786 (2009)10.1111/j.1462-2920.2010.02264.x
2008
Glycosyltransferases, glycoside hydrolases: surprise, surprise!
Bernard Henrissat, Gerlind Sulzenbacher, Yves Bourne
Current Opinion in Structural Biology (2008)10.1016/j.sbi.2008.09.003
Glycosyltransferases, glycoside hydrolases: surprise, surprise!
Bernard Henrissat, Gerlind Sulzenbacher, Yves Bourne
Current Opinion in Structural Biology 18:527-533 (2008)10.1016/j.sbi.2008.09.003
2007
Structural Analysis of the Synaptic Protein Neuroligin and Its β-Neurexin Complex: Determinants for Folding and Cell Adhesion
Igor Fabrichny, Philippe Leone, Gerlind Sulzenbacher, Davide Comoletti, Meghan Miller, Palmer Taylor, Yves Bourne, Pascale Marchot
Neuron 56:979-991 (2007)10.1016/j.neuron.2007.11.013
Des glycosidases bactériennes pour du sang universel
Gerlind Sulzenbacher, Yves Bourne, Bernard Henrissat
Médecine/Sciences (2007)10.1051/medsci/20072389703
Bacterial glycosidases for the production of universal red blood cells
Qiyong P Liu, Gerlind Sulzenbacher, Huaiping Yuan, Eric P Bennett, Greg Pietz, Kristen Saunders, Jean Spence, Edward Nudelman, Steven B Levery, Thayer White, John M Neveu, William S Lane, Yves Bourne, Martin L Olsson, Bernard Henrissat, Henrik Clausen
Nature Biotechnology (2007)10.1038/nbt1298
Gene overexpression and biochemical characterization of the biotechnologically relevant chlorogenic acid hydrolase from Aspergillus niger
Isabelle Benoit, Michèle Asther, Yves Bourne, David Navarro, Stéphane Canaan, Laurence Lesage-Meessen, Marga Herweijer, Pedro M Coutinho, Marcel M. Asther, Eric Record
Applied and Environmental Microbiology 73:5624-5632 (2007)10.1128/AEM.00374-07
2006
Substrate and product trafficking through the active center gorge of acetylcholinesterase analyzed by crystallography and equilibrium binding.
Yves Bourne, Zoran Radic, Gerlind Sulzenbacher, Esther Kim, Palmer Taylor, Pascale Marchot
Journal of Biological Chemistry 281:29256-67 (2006)10.1074/jbc.M603018200
Functional characterization of the glycosyltransferase domain of penicillin-binding protein 1a from Thermotoga maritima
Julien Offant, François Michoux, Annabelle Dermiaux, Jacques Biton, Yves Bourne
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1764:1036-1042 (2006)10.1016/j.bbapap.2006.03.012
LppX is a lipoprotein required for the translocation of phthiocerol dimycocerosates to the surface of Mycobacterium tuberculosis
Gerlind Sulzenbacher, Stéphane Canaan, Yann Bordat, Olivier Neyrolles, Gustavo Stadthagen, Véronique Roig-Zamboni, Jean Rauzier, Damien Maurin, Françoise Laval, Mamadou Daffé, Christian Cambillau, Brigitte Gicquel, Yves Bourne, Mary Jackson
EMBO Journal (2006)10.1038/sj.emboj.7601048
Structural comparison of three crystalline complexes of a peptidic toxin with a synaptic acetylcholine recognition protein.
Yves Bourne, Scott B Hansen, Gerlind Sulzenbacher, Todd T Talley, Tom Huxford, Palmer Taylor, Pascale Marchot
Journal of Molecular Neuroscience 30:103-4 (2006)
Structural characterization of agonist and antagonist-bound acetylcholine-binding protein from Aplysia californica.
Scott B Hansen, Gerlind Sulzenbacher, Tom Huxford, Pascale Marchot, Yves Bourne, Palmer Taylor
Journal of Molecular Neuroscience 30:101-2 (2006)
2005
A. niger protein “EstA”, perhaps a new electrotactin, defines a new class of fungal esterases within the α/β hydrolase fold superfamily
Yves Bourne, Alinda Hasper, Henri Chahinian, Ludovic Renault, Marianick Juin, Leo de Graaff, Pascale Marchot
Chemico-Biological Interactions 157-158:395-396 (2005)10.1016/j.cbi.2005.10.072
Structural insights into AChE inhibition by monoclonal antibodies
Ludovic Renault, Sosthène Essono, Marianick Juin, Didier Boquet, Jacques Grassi, Yves Bourne, Pascale Marchot
Chemico-Biological Interactions 157-158:397-400 (2005)10.1016/j.cbi.2005.10.073
Crystal structure of the conserved hypothetical protein Rv1155 from Mycobacterium tuberculosis
Stéphane Canaan, Gerlind Sulzenbacher, Véronique Roig-Zamboni, Loréna Scappuccini-Calvo, Frédéric Frassinetti, Damien Maurin, Christian Cambillau, Yves Bourne, Christian Griesinger
FEBS Letters (2005)10.1016/j.febslet.2004.11.069
Crystal structure of a Cbtx-AChBP complex reveals essential interactions between snake alpha-neurotoxins and nicotinic receptors,
Yves Bourne, T. T. Talley, Hansen S. B., P. Taylor, Pascale Marchot
EMBO Journal 24:1512-1522 (2005)
Structural insights into conformational flexibility at the peripheral site and within the active center gorge of AChE, Chem Biol Interact. 157-158, 159-65.
Yves Bourne, Z. Radic, Kolb H. C., Sharpless K. B., P. Taylor, Pascale Marchot
Chemico-Biological Interactions 157-158:159-65. (2005)
Structures of Aplysia AChBP complexes with nicotinic agonists and antagonists reveal distinctive binding interfaces and conformations
Scott B Hansen, Gerlind Sulzenbacher, Tom Huxford, Pascale Marchot, Palmer Taylor, Yves Bourne
EMBO Journal 24:3635-3646 (2005)10.1038/sj.emboj.7600828
2004
Aspergillus niger Protein EstA Defines a New Class of Fungal Esterases within the α/β Hydrolase Fold Superfamily of Proteins
Yves Bourne, Alinda A Hasper, Henri Chahinian, Marianick Juin, Leo H de Graaff, Pascale Marchot
Structure 12:677-687 (2004)10.1016/j.str.2004.03.005
Crystal Structure of Thermotoga maritima ␣-L-Fucosidase INSIGHTS INTO THE CATALYTIC MECHANISM AND THE MOLECULAR BASIS FOR FUCOSIDOSIS
Gerlind Sulzenbacher, Christophe Bignon, Takeshi Nishimura, Chris A Tarling, Stephen G Withers, Bernard Henrissat, Yves Bourne
Journal of Biological Chemistry (2004)10.1074/jbc.M313783200
Freeze-frame inhibitor captures acetylcholinesterase in a unique conformation
Y. Bourne, H. Kolb, Z. Radic, K. Sharpless, P. Taylor, Pascale Marchot
Proceedings of the National Academy of Sciences of the United States of America 101:1449-1454 (2004)10.1073/pnas.0308206100
Crystal Structure of E. coli YddE Protein Reveals a Striking Homology With Diaminopimelate Epimerase
Alice Grassick, Gerlind Sulzenbacher, Véronique Roig-Zamboni, Valérie Campanacci, Christian Cambillau, Yves Bourne
Proteins - Structure, Function and Bioinformatics 55:764-767 (2004)10.1002/prot.20025
2003
Identification of the Catalytic Nucleophile of the Family 29 α-L-Fucosidase from Thermotoga maritima through Trapping of a Covalent Glycosyl-Enzyme Intermediate and Mutagenesis
Chris A Tarling, Shouming He, Gerlind Sulzenbacher, Christophe Bignon, Yves Bourne, Bernard Henrissat, Stephen G Withers
Journal of Biological Chemistry (2003)10.1074/jbc.M306610200
Structural insights into ligand interactions at the acetylcholinesterase peripheral anionic site
Yves Bourne, Palmer Taylor, Zoran Radic, Pascale Marchot
EMBO Journal 22:1-12 (2003)10.1093/emboj/cdg005
2002
Mechanism of Acetylcholinesterase Inhibition by Fasciculin: A 5-ns Molecular Dynamics Simulation
Kaihsu Tai, Tongye Shen, Richard Henchman, Yves Bourne, Pascale Marchot, J. Andrew Mccammon
Journal of the American Chemical Society 124:6153-6161 (2002)10.1021/ja017310h
A medium-throughput crystallization approach
Gerlind Sulzenbacher, Arnaud Gruez, Véronique Roig-Zamboni, Silvia Spinelli, Christel Valencia, Fabienne Pagot, Renaud Vincentelli, Christophe Bignon, Aurelia Salomoni, Sacha Grisel, Damien Maurin, Céline Huyghe, Kent Johansson, Alice Grassick, Alain Roussel, Yves Bourne, Sophie Perrier, Linda Miallau, Phillippe Cantau, Eric Blanc, Michel Genevois, Alain Grossi, André Zenatti, Valérie Campanacci, Christian Cambillau
Acta crystallographica Section D : Structural biology [1993-...] 58:2109-2115 (2002)10.1107/s0907444902013938
2001
Crystal Structure of Streptococcus pneumoniae N -Acetylglucosamine-1-phosphate Uridyltransferase Bound to Acetyl-coenzyme A Reveals a Novel Active Site Architecture
Gerlind Sulzenbacher, Laurent Gal, Caroline Peneff, Florence Fassy, Yves Bourne
Journal of Biological Chemistry 276:11844-11851 (2001)10.1074/jbc.M011225200
2000
Immunocytochemical Localization and Crystal Structure of Human Frequenin (Neuronal Calcium Sensor 1)
Yves Bourne, Jens Dannenberg, Verena Pollmann, Pascale Marchot, Olaf Pongs
Journal of Biological Chemistry 276:11949-11955 (2000)10.1074/jbc.m009373200
1999
Conformational Flexibility of the Acetylcholinesterase Tetramer Suggested by X-ray Crystallography*
Yves Bourne, Jacques Grassi, Pierre E Bougis, Pascale Marchot
Journal of Biological Chemistry 274:30370-30376 (1999)
Crystal Structure of Mouse Acetylcholinesterase
Yves Bourne, Palmer Taylor, Pierre E Bougis, Pascale Marchot
Journal of Biological Chemistry 274:2963-2970 (1999)10.1074/jbc.274.5.2963
1998
Inhibition of mouse acetylcholinesterase by fasciculin: Crystal structure of the complex and mutagenesis of fasciculin
Pascale Marchot, Yves Bourne, Claudine Prowse, Pierre Bougis, Palmer Taylor
Toxicon 36:1613-1622 (1998)10.1016/s0041-0101(98)00154-8
Barley alpha-amylase bound to its endogenous protein inhibitor BASI: crystal structure of the complex at 1.9 A resolution.
F. Vallee, A. Kadziola, Yves Bourne, M. Juy, Kw Rodenburg, B. Svensson, R. Haser
Structure 6:649-659 (1998)
1996
Soluble monomeric acetylcholinesterase from mouse: Expression, purification, and crystallization in complex with fasciculin
Pascale Marchot, Raimond B.G. Ravelli, Mia Raves, Yves Bourne, Daniel Vellom, Joan Kanter, Shelley Camp, Joel Sussman, Palmer Taylor
Protein Science 5:672-679 (1996)10.1002/pro.5560050411
1995
Acetylcholinesterase Inhibition by Fasciculin: Crystal Structure of the Complex
Yves Bourne, Palmer Taylor, Pascale Marchot
Cell 83:503-512 (1995)10.1016/0092-8674(95)90128-0
1994
Structures of a legume lectin complexed with the human lactotransferrin N2 fragment, and with an isolated biantennary glycopeptide: role of the fucose moiety.
Yves Bourne, J. Mazurier, Delphine Legrand, Pierre Rougé, J. Montreuil, G. Spik, C. Cambillau
Structure / Struct Fold Des; Structure (Camb ) 2:209-19 (1994)
Characterization, crystallization and preliminary X-ray crystallographic analysis of the complex between barley alpha-amylase and the bifunctional alpha-amylase/subtilisin inhibitor from barley seeds.
F. Vallee, A. Kadziola, Yves Bourne, J. Abe, B. Svensson, R. Haser
Journal of Molecular Biology 236:368-371 (1994)
Primary and three-dimensional structure of lactotransferrin (lactoferrin) glycans.
G. Spik, B. Coddeville, J. Mazurier, Yves Bourne, C. Cambillaut, J. Montreuil
Advances in Experimental Medicine and Biology 357:21-32 (1994)
1992
Crystallization and preliminary X-ray diffraction study of Lathyrus ochrus isolectin II complexed to the human lactotransferrin N2 fragment.
Yves Bourne, M. P. Nésa, Pierre Rougé, J. Mazurier, Delphine Legrand, G. Spik, J. Montreuil, C. Cambillau
Journal of Molecular Biology 227:938-41 (1992)
1989
Crystallization and preliminary X-ray study of horse pancreatic lipase
Dominique Lombardo, Catherine Chapus, Yves Bourne, Christian Cambillau
Journal of Molecular Biology 205:259-261 (1989)10.1016/0022-2836(89)90380-x
1988
Crystallization and preliminary X-ray studies of two isolectins from the seeds of Lathyrus ochrus
Yves Bourne, Pierre Rougé, Christian Cambillau
Journal of Molecular Biology 202:685-687 (1988)10.1016/0022-2836(88)90299-9
