Personne Compétente en Radioprotection

Research Associate (IR1 Aix Marseille University) team Dr. Bruno Canard and screening platform Marseille-Luminy (PCML)

Research subject: Activity and inhibition studies of viral replicative enzymes

Experience:

  • 1990 PhD in Biochemistry Martin-Luther-Universität Halle/Germany
  • 1991 Postdoctoral Fellow Humboldt-Universität Berlin/Germany
  • 1992 to 1993 Postdoctoral Fellow at the University of London/United Kingdom supported by the British Council and the German Academic Exchange Service
  • 1994 to 2000 Postdoctoral Fellow at the Universidad National Autonoma de Mexico supported in part by the Humboldt-Foundation Germany
  • 2001 to date AFMB Marseille

Publications

2022

2022
A dual mechanism of action of AT-527 against SARS-CoV-2 polymerase

Ashleigh Shannon, Véronique Fattorini, Bhawna Sama, Barbara Selisko, Mikael Feracci, Camille Falcou, Pierre Gauffre, Priscila El Kazzi, Adrien Delpal, Etienne Decroly, Karine Alvarez, Cécilia Eydoux, Jean-Claude Guillemot, Adel Moussa, Steven Good, Paolo La Colla, Kai Lin, Jean-Pierre Sommadossi, Yingxiao Zhu, Xiaodong Yan, Hui Shi, Francois Ferron, Bruno Canard

Nature Communications 13 (2022)10.1038/s41467-022-28113-1

2021

2021
Simeprevir Potently Suppresses SARS-CoV-2 Replication and Synergizes with Remdesivir

Ho Sing Lo, Kenrie Pui Yan Hui, Hei-Ming Lai, Xu He, Khadija Shahed Khan, Simranjeet Kaur, Junzhe Huang, Zhongqi Li, Anthony Chan, Hayley Hei-Yin Cheung, Ka-Chun Ng, John Chi Wang Ho, Yu Wai Chen, Bowen Ma, Peter Man-Hin Cheung, Donghyuk Shin, Kaidao Wang, Meng-Hsuan Lee, Barbara Selisko, Cecilia Eydoux, Jean-Claude Guillemot, Bruno Canard, Kuen-Phon Wu, Po-Huang Liang, Ivan Dikic, Zhong Zuo, Francis Chan, David Hui, Vincent Mok, Kam-Bo Wong, Chris Ka Pun Mok, Ho Ko, Wei Shen Aik, Michael Chi Wai Chan, Wai-Lung Ng

ACS Central Science 7:792-802 (2021)10.1021/acscentsci.0c01186

2021
Dengue Virus NS5 Transcribes Metabolite-Capped, RIG-I Sensitive vRNAs

Brandon Schweibenz, Mihai Solotchi, Etienne Decroly, Barbara Selisko, Bruno Canard, Smita Patel

Biophysical Journal 120:135a (2021)10.1016/j.bpj.2020.11.1017

2020

2020
Synthesis and biological evaluation of novel flexible nucleoside analogues that inhibit flavivirus replication in vitro

Joy Thames, Charles Waters, Coralie Valle, Marcella Bassetto, Wahiba Aouadi, Baptiste Martin, Barbara Selisko, Arissa Falat, Bruno Coutard, Andrea Brancale, Bruno Canard, Etienne Decroly, Katherine Seley-Radtke

Bioorganic and Medicinal Chemistry Letters 28:115713 (2020)10.1016/j.bmc.2020.115713

2020
Rapid incorporation of Favipiravir by the fast and permissive viral RNA polymerase complex results in SARS-CoV-2 lethal mutagenesis

Ashleigh Shannon, Barbara Selisko, Nhung-Thi-Tuyet Le, Johanna Huchting, Franck Touret, Géraldine Piorkowski, Véronique Fattorini, Francois Ferron, Etienne Decroly, Chris Meier, Bruno Coutard, Olve Peersen, Bruno Canard

Nature Communications 11:4682 (2020)10.1038/s41467-020-18463-z

2020
Remdesivir and SARS-CoV-2: Structural requirements at both nsp12 RdRp and nsp14 Exonuclease active-sites

Ashleigh Shannon, Nhung Thi-Tuyet Le, Barbara Selisko, Cecilia Eydoux, Karine Alvarez, Jean-Claude Guillemot, Etienne Decroly, Olve Peersen, Francois Ferron, Bruno Canard

Antiviral Research 178:104793 (2020)10.1016/j.antiviral.2020.104793

2018

2018
Structural and Functional Basis of the Fidelity of Nucleotide Selection by Flavivirus RNA-Dependent RNA Polymerases

Barbara Selisko, Nicolas Papageorgiou, Francois Ferron, Bruno Canard

Viruses 10:59 (2018)10.3390/v10020059

2017

2017
Substrate selectivity of Dengue and Zika virus NS5 polymerase towards 2′-modified nucleotide analogues

Supanee Potisopon, Francois Ferron, Véronique Fattorini, Barbara Selisko, Bruno Canard

Antiviral Research 140:25-36 (2017)10.1016/j.antiviral.2016.12.021

2017
Zika Virus Methyltransferase: Structure and Functions for Drug Design Perspectives

Bruno Coutard, Karine Barral, Julie Lichiere, Barbara Selisko, Baptiste Martin, Wahiba Aouadi, Miguel Ortiz Lombardia, Françoise Debart, Jean-Jacques Vasseur, Jean Claude Guillemot, Bruno Canard, Etienne Decroly

Journal of Virology 91:2202-2218 (2017)10.1128/JVI.02202-16

2016

2016
The methyltransferase domain of dengue virus protein NS5 ensures efficient RNA synthesis initiation and elongation by the polymerase domain (vol 42, pg 11642, 2014)

Supanee Potisopon, Stephane Priet, Axelle Collet, Etienne Decroly, Bruno Canard, Barbara Selisko

Nucleic Acids Research 44:2974 (2016)10.1093/nar/gkv1294

2015

2015
The RNA Template Channel of the RNA-Dependent RNA Polymerase as a Target for Development of Antiviral Therapy of Multiple Genera within a Virus Family

Lonneke van der Linden, Laia Vives-Adrián, Barbara Selisko, Cristina Ferrer-Orta, Xinran Liu, Kjerstin Lanke, Rachel Ulferts, Armando M. de Palma, Federica Tanchis, Nesya Goris, David Lefebvre, Kris de Clercq, Pieter Leyssen, Céline Lacroix, Gerhard Pürstinger, Bruno Coutard, Bruno Canard, David D. Boehr, Jamie J. Arnold, Craig E. Cameron, Nuria Verdaguer, Johan Neyts, Frank J. M van Kuppeveld

PLoS Pathogens 11 (2015)10.1371/journal.ppat.1004733.s010

2014

2014
The methyltransferase domain of dengue virus protein NS5 ensures efficient RNA synthesis initiation and elongation by the polymerase domain

Supanee Potisopon, Stéphane Priet, Axelle Collet, Etienne Decroly, Bruno Canard, Barbara Selisko

Nucleic Acids Research 42 (2014)10.1093/nar/gku666

2012

2012
Molecular Basis for Nucleotide Conservation at the Ends of the Dengue Virus Genome

Barbara Selisko, Supanee Potisopon, Rym Agred, Stephane Priet, Isabelle Varlet, Yann Thillier, Corinne Sallamand, Françoise Debart, Jean-Jacques Vasseur, Bruno Canard

PLoS Pathogens 8:e1002912 (2012)10.1371/journal.ppat.1002912

2007

2007
Crystal Structure of the RNA Polymerase Domain of the West Nile Virus Non-structural Protein 5

Hélène Malet, Marie-Pierre Egloff, Barbara Selisko, Rebecca Butcher, Peter Wright, Michael Roberts, Arnaud Gruez, Gerlind Sulzenbacher, Clemens Vonrhein, Gérard Bricogne, Jason Mackenzie, Alexander Khromykh, Andrew Davidson, Bruno Canard

Journal of Biological Chemistry (2007)10.1074/jbc.M607273200

2006

2006
Comparative mechanistic studies of de novo RNA synthesis by flavivirus RNA-dependent RNA polymerases

Barbara Selisko, Hélène Dutartre, Jean-Claude Guillemot, Claire Debarnot, Delphine Benarroch, Alexander Khromykh, Philippe Desprès, Marie-Pierre Egloff, Bruno Canard

Virology 351:145-158 (2006)10.1016/j.virol.2006.03.026