Service : Production dans E.coli, purification et interaction de protéines à haut-débit

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Publications

2021

2021
Display of the human mucinome with defined O-glycans by gene engineered cells

Rebecca Nason, Christian Büll, Andriana Konstantinidi, Lingbo Sun, Zilu Ye, Adnan Halim, Wenjuan Du, Daniel Sørensen, Fabien Durbesson, Sanae Furukawa, Ulla Mandel, Hiren Joshi, Leo Alexander Dworkin, Lars Hansen, Leonor David, Tina Iverson, Barbara Bensing, Paul Sullam, Ajit Varki, Erik De Vries, Cornelis de Haan, Renaud Vincentelli, Bernard Henrissat, Sergey Vakhrushev, Henrik Clausen, Yoshiki Narimatsu

Nature Communications 12:4070 (2021)10.1038/s41467-021-24366-4

2021
Endonuclease-based genotyping of the RBM as a method to track the emergence or evolution of SARS-CoV-2 variants

Eva Lopez, Margot Barthélémy, Cécile Baronti, Shirley Masse, Alessandra Falchi, Fabien Durbesson, Renaud Vincentelli, Xavier de Lamballerie, Rémi Charrel, Bruno Coutard

iScience 24:103329 (2021)10.1016/j.isci.2021.103329

2021
Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms

Francois Iv, Carla Silva Martins, Gerard Castro-Linares, Cyntia Taveneau, Pascale Barbier, Pascal Verdier-Pinard, Luc Camoin, Stéphane Audebert, Feng-Ching Tsai, Laurie Ramond, Alex Llewellyn, Mayssa Belhabib, Koyomi Nakazawa, Aurelie Di Cicco, Renaud Vincentelli, Jérôme Wenger, Stéphanie Cabantous, Gijsje Koenderink, Aurélie Bertin, Manos Mavrakis, Carla Silva Martins

Journal of Cell Science 134:jcs258484 (2021)10.1242/jcs.258484

2021
Community-Wide Experimental Evaluation of the PROSS Stability-Design Method

Yoav Peleg, Renaud Vincentelli, Brett Collins, Kai-En Chen, Emma Livingstone, Saroja Weeratunga, Natalya Leneva, Qian Guo, Kim Remans, Kathryn Perez, Gro E.K Bjerga, Øivind Larsen, Ondřej Vaněk, Ondřej Skořepa, A. Poterszman, Sophie Jacquemin, Svend Kjaer, Evangelos Christodoulou, Shira Albeck, Orly Dym, Elena Ainbinder, Tamar Unger, Anja Schuetz, Susann Matthes, Michael Bader, Ario de Marco, Paola Storici, Marta Semrau, Peggy Stolt-Bergner, Christian Aigner, Sabine Suppmann, Adi Goldenzweig, Sarel Fleishman

Journal of Molecular Biology 433:166964 (2021)10.1016/j.jmb.2021.166964

2021
Host PDZ‐containing proteins targeted by SARS‐CoV‐2

Célia Caillet-Saguy, Fabien Durbesson, Veronica Rezelj, Gergö Gogl, Quang Dinh Tran, Jean‐claude Twizere, Marco Vignuzzi, Renaud Vincentelli, Nicolas Wolff

FEBS Journal epub (2021)10.1111/febs.15881

2021
A High-Affinity Peptide Ligand Targeting Syntenin Inhibits Glioblastoma

Linda Haugaard-Kedström, Louise Clemmensen, Vita Sereikaite, Zeyu Jin, Eduardo Fernandes, Bianca Wind, Flor Abalde-Gil, Jan Daberger, Maria Vistrup-Parry, Diana Aguilar-Morante, Raphael Leblanc, Antonio Egea-Jimenez, Marte Albrigtsen, Kamilla. Jensen, Thomas Jensen, Ylva Ivarsson, Renaud Vincentelli, Petra Hamerlik, Jeanette Hammer Andersen, Pascale Zimmermann, Weontae Lee, Kristian Strømgaard

Journal of Medicinal Chemistry 64:1423-1434 (2021)10.1021/acs.jmedchem.0c00382

2020

2020
Interactomic affinity profiling by holdup assay: Acetylation and distal residues impact the PDZome-binding specificity of PTEN phosphatase

Pau Jané, Gergő Gógl, Camille Kostmann, Goran Bich, Virginie Girault, Célia Caillet-Saguy, Pascal Eberling, Renaud Vincentelli, Nicolas Wolff, Gilles Travé, Yves Nominé

PLoS ONE 15:e0244613 (2020)10.1371/journal.pone.0244613

2020
Display of the Human Mucinome With Defined O-Glycans by Gene Engineered Cells

Nason Rebecca, Bull, Christian, Hiren J Joshi, Konstantinidi, Andriana, Renaud Vincentelli, Bernard Henrissat, Vakhrushev, Sergey, Henrik Clausen, Yoshiki Narimatsu

Glycobiology (2020)

2020
A Venomics Approach Coupled to High-Throughput Toxin Production Strategies Identifies the First Venom-Derived Melanocortin Receptor Agonists

Steve Reynaud, Justyna Ciolek, Michel Degueldre, Natalie Saez, Ana Filipa Sequeira, Yoan Duhoo, Joana Brás, Herve Meudal, Miguel Cabo Díez, Victoria Fernández Pedrosa, Marion Verdenaud, Julia Boeri, Oscar Pereira Ramos, Frédéric Ducancel, Margot Vanden Driessche, Rudy Fourmy, Aude Violette, Grégory Upert, Gilles Mourier, Annette Beck-Sickinger, Karin Mörl, Celine Landon, Carlos Fontes, Rebeca Miñambres Herráiz, Ricardo Rodríguez de la Vega, Steve Peigneur, Jan Tytgat, Loïc Quinton, Edwin de Pauw, Renaud Vincentelli, Denis Servent, Nicolas Gilles

Journal of Medicinal Chemistry 63:8250-8264 (2020)10.1021/acs.jmedchem.0c00485

2020
Dual Specificity PDZ- and 14-3-3-Binding Motifs: A Structural and Interactomics Study

Gergo Gogl, Pau Jane, Célia Caillet-Saguy, Camille Kostmann, Goran Bich, Alexandra Cousido-Siah, Laszlo Nyitray, Renaud Vincentelli, Nicolas Wolff, Yves Nominé, Nikolai Sluchanko, Gilles Travé

Structure (2020)10.1016/j.str.2020.03.010

2020
Dual Specificity PDZ- and 14-3-3-Binding Motifs: A Structural and Interactomics Study

Gergo Gogl, Pau Jane, Célia Caillet-Saguy, Camille Kostmann, Goran Bich, Alexandra Cousido-Siah, Laszlo Nyitray, Renaud Vincentelli, Nicolas Wolff, Yves Nominé, Nikolai Sluchanko, Gilles Travé

Structure 28:747 (2020)10.1016/j.str.2020.03.010

2019

2019
Ubiquitous Carbohydrate Binding Modules Decorate 936 Lactococcal Siphophage Virions

Stephen Hayes, Jennifer Mahony, Renaud Vincentelli, Laurie Ramond, Arjen Nauta, Douwe van Sinderen, Christian Cambillau

Viruses 11:631 (2019)10.3390/v11070631

2019
Structure-based optimization of a PDZ binding motif within a viral peptide stimulates neurite outgrowth

Zakir Khan, Elouan Terrien, Florent Delhommel, Cynthia Lefebvre-Omar, Delphine Bohl, Sandrine Vitry, Clara Bernard, Juan Ramirez, Alain Chaffotte, Kevin Ricquier, Renaud Vincentelli, Henri Buc, Christophe Préhaud, Nicolas Wolff, Monique Lafon

Journal of Biological Chemistry 294:137555-13768 (2019)10.1074/jbc.RA119.008238

2019
Sizes of actin networks sharing a common environment are determined by the relative rates of assembly

Adrien Antkowiak, Audrey Guillotin, Micaela Boiero Sanders, Jessica Colombo, Renaud Vincentelli, Alphee Michelot

PLoS Biology 17:e3000317 (2019)10.1371/journal.pbio.3000317

2019
Rewiring of RSK–PDZ Interactome by Linear Motif Phosphorylation

Gergő Gógl, Beáta Biri-Kovács, Fabien Durbesson, Pau Jane, Yves Nomine, Camille Kostmann, Viktória Bilics, Márton Simon, Attila Reményi, Renaud Vincentelli, Gilles Trave, László Nyitray

Journal of Molecular Biology 431:1234-1249 (2019)10.1016/j.jmb.2019.01.038

2018

2018
Ancient acquisition of “alginate utilization loci” by human gut microbiota

Sophie Mathieu, Mélanie Touvrey-Loiodice, Laurent Poulet, Sophie Drouillard, Renaud Vincentelli, Bernard Henrissat, Gudmund Skjåk-Bræk, William Helbert

Scientific Reports 8:8075 (2018)10.1038/s41598-018-26104-1

2018
Functional carbohydrate binding modules identified in evolved dits from siphophages infecting various Gram-positive bacteria

Stephen Hayes, Renaud Vincentelli, Jennifer Mahony, Arjen Nauta, Laurie Ramond, Gabriele Lugli, Marco Ventura, Douwe van Sinderen, Christian Cambillau

Molecular Microbiology 110:777-795 (2018)10.1111/mmi.14124

2018
Rewiring of RSK-PDZ Interactome by Linear Motif Phosphorylation.

Gergő Gógl, Beáta Biri-Kovács, Fabien Durbesson, Pau Jane, Yves Nominé, Camille Kostmann, Viktória Bilics, Márton Simon, Attila Reményi, Renaud Vincentelli, Gilles Travé, László Nyitray

Journal of Molecular Biology 17:1234-1249 (2018)10.1016/j.jmb.2019.01.038

2018
A gated relaxation oscillator mediated by FrzX controls morphogenetic movements in Myxococcus xanthus

Mathilde Guzzo, Seán Murray, Eugenie Martineau, Sebastien Lhospice, Gregory Baronian, Laetitia My, Yong Zhang, Leon Espinosa, Renaud Vincentelli, Benjamin Bratton, Joshua Shaevitz, Virginie Molle, Martin Howard, Tam Mignot

Nature Microbiology 3:948-959 (2018)10.1038/s41564-018-0203-x

2018
Glycosylate and move! The glycosyltransferase Maf is involved in bacterial flagella formation

Gerlind Sulzenbacher, Veronique Roig-Zamboni, Régine Lebrun, Yann Guérardel, Dorothée Murat, Pascal Mansuelle, Nao Yamakawa, Xin-Xin Qian, Renaud Vincentelli, Yves Bourne, Long-Fei Wu, François Alberto

Environmental Microbiology 20:228-240 (2018)10.1111/1462-2920.13975

2018
ANISEED 2017: extending the integrated ascidian database to the exploration and evolutionary comparison of genome-scale datasets

Matija Brozovic, Christelle Dantec, Justine Dardaillon, Delphine Dauga, Emmanuel Faure, Mathieu Gineste, Alexandra Louis, Magali Naville, Kazuhiro R. Nitta, Jacques Piette, Wendy Reeves, Celine Scornavacca, Paul Simion, Renaud Vincentelli, Maëlle Bellec, Sameh Ben Aicha, Marie Fagotto, Marion Gueroult-Bellone, Maximilian Haeussler, Edwin Jacox, Elijah K. Lowe, Mickaël Mendez, Alexis Roberge, Alberto Stolfi, Rui Yokomori, C. Titus Brown, Christian Cambillau, Lionel Christiaen, Frédéric Delsuc, Emmanuel Douzery, Rémi Dumollard, Takehiro Kusakabe, Kenta Nakai, Hiroki Nishida, Yutaka Satou, Billie Swalla, Michael Veeman, Jean-Nicolas Volff, Patrick Lemaire

Nucleic Acids Research 46:D718-D725 (2018)10.1093/nar/gkx1108

2017

2017
OrfX, a Nucleomodulin Required for Listeria monocytogenes Virulence

Andrzej Prokop, Edith Gouin, Veronique Villiers, Marie-Anne Nahori, Renaud Vincentelli, Mélodie Duval, Pascale Cossart, Olivier Dussurget

mBio 8:e01550-17 (2017)10.1128/mBio.01550-17

2017
High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery

Jeremy Turchetto, Ana Filipa Sequeira, Laurie Ramond, Fanny Peysson, Joana Brás, Natalie Saez, Yoan Duhoo, Marilyne Blémont, Catarina Guerreiro, Loïc Quinton, Edwin de Pauw, Nicolas Gilles, Hervé Darbon, Carlos Fontes, Renaud Vincentelli

Microbial Cell Factories 16:6 (2017)10.1186/s12934-016-0617-1

2017
Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli

Ana Filipa Sequeira, Jeremy Turchetto, Natalie Saez, Fanny Peysson, Laurie Ramond, Yoan Duhoo, Marilyne Blémont, Vânia Fernandes, Luís Gama, Luis Ferreira, Catarina Guerreiro, Nicolas Gilles, Hervé Darbon, Carlos Fontes, Renaud Vincentelli

Microbial Cell Factories 16:4 (2017)10.1186/s12934-016-0618-0

2016

2016
Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins

Ana Filipa Sequeira, Joana L. A. Bras, Catarina I. P. D. Guerreiro, Renaud Vincentelli, Carlos M. G. A. Fontes

BMC Biotechnology 16 (2016)10.1186/s12896-016-0316-3

2016
T7 Endonuclease I Mediates Error Correction in Artificial Gene Synthesis

Ana Filipa Sequeira, Catarina I. P. D. Guerreiro, Renaud Vincentelli, Carlos M. G. A. Fontes

Molecular Biotechnology 58:573-584 (2016)10.1007/s12033-016-9957-7

2016
Differential conformational modulations of MreB folding upon interactions with GroEL/ES and TRiC chaperonin components

S. Moparthi, Uno Carlsson, Renaud Vincentelli, Bengt-Harald Jonsson, Per Hammarström, Jérôme Wenger

Scientific Reports 6:28386 (2016)10.1038/srep28386

2015

2015
Quantifying domain-ligand affinities and specificities by high-throughput holdup assay

Renaud Vincentelli, Katja Luck, Juline Poirson, Jolanta Polanowska, Julie Abdat, Marilyne Blémont, Jeremy Turchetto, François Iv, Kevin Ricquier, Marie-Laure Straub, Anne Forster, Patricia Cassonnet, Jean-Paul Borg, Yves Jacob, Murielle Masson, Yves Nominé, Jérôme Reboul, Nicolas Wolff, Sebastian Charbonnier, Gilles Travé

Nature Methods 12:787-793 (2015)10.1038/nmeth.3438

2015
Quantifying domain-ligand affinities and specificities by high-throughput holdup assay

Renaud Vincentelli, Katja Luck, Juline Poirson, Jolanta Polanowska, Julie Abdat, Marilyne Blémont, Jeremy Turchetto, François Iv, Kevin Ricquier, Marie-Laure Straub, Anne Forster, Patricia Cassonnet, Jean-Paul Borg, Yves Jacob, Murielle Masson, Yves Nominé, Jérome Reboul, Nicolas Wolff, Sebastian Charbonnier, Gilles Travé

Nature Methods 12:787-793 (2015)10.1038/nmeth.3438

2015
A Flexible Extension of the Drosophila Ultrabithorax Homeodomain Defines a Novel Hox/PBC Interaction Mode

Nicolas Foos, Corinne Maurel-Zaffran, Maria Jesus Mate, Renaud Vincentelli, Matthieu Hainaut, Helene Berenger, Jacques Pradel, Andrew J. Saurin, Miguel Ortiz-Lombardia, Yacine Graba

Structure 23:270-279 (2015)10.1016/j.str.2014.12.011

2014

2014
Generation of a vector suite for protein solubility screening

Agustín Correa, Claudia Ortega, Gonzalo Obal, Pedro Alzari, Renaud Vincentelli, Pablo Oppezzo

Frontiers in Microbiology 5:67 (2014)10.3389/fmicb.2014.00067

2013

2013
Crystallization and preliminary X-ray analysis of monalysin, a novel β-pore-forming toxin from the entomopathogen Pseudomonas entomophila

Philippe Leone, Maryline Blemont, Renaud Vincentelli, Christine Kellenberger, Onya Opota, Bruno Lemaitre, Alain Roussel

Acta Crystallographica Section F: Structural Biology and Crystallization Communications 69:930-933 (2013)10.1107/S174430911301885X

2013
High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli

Hervé Nozach, Carole Fruchart-Gaillard, François Fenaille, Fabrice Beau, Oscar Pereira Ramos, Badreddine Douzi, Natalie Saez, Mireille Moutiez, Denis Servent, Muriel Gondry, Robert Thai, Philippe Cuniasse, Renaud Vincentelli, Vincent Dive

Microbial Cell Factories 12 (2013)10.1186/1475-2859-12-37

2011

2011
Characterization of rickettsial adhesin Adr2 belonging to a new group of adhesins in α-proteobacteria

Manohari Vellaiswamy, Malgorzata Kowalczewska, Vicky Merhej, Claude Nappez, Renaud Vincentelli, Patricia Renesto, Didier Raoult

Microbial Pathogenesis 50:233-242 (2011)10.1016/j.micpath.2011.01.009

2011
The Drosophila peptidoglycan-recognition protein LF interacts with peptidoglycan-recognition protein LC to downregulate the Imd pathway

Nada Basbous, Franck Coste, Philippe Leone, Renaud Vincentelli, Julien Royet, Christine Kellenberger, Alain Roussel

EMBO Reports 12:327-333 (2011)10.1038/embor.2011.19

2009

2009
Mammalian G-protein-coupled receptor expression in Escherichia coli: I. High-throughput large-scale production as inclusion bodies

Kerstin Michalke, Marie-Eve Gravière, Céline Huyghe, Renaud Vincentelli, Renaud Wagner, Franc Pattus, Kathrin Schroeder, Jan Oschmann, Rainer Rudolph, Christian Cambillau, Aline Desmyter

Analytical Biochemistry 386:147-155 (2009)10.1016/j.ab.2008.12.016

2009
Production and biophysical characterization of the CorA transporter from Methanosarcina mazei.

David Veesler, Stéphanie Blangy, Marina Siponen, Renaud Vincentelli, Christian Cambillau, Giuliano Sciara

Analytical Biochemistry 388:115-21 (2009)10.1016/j.ab.2009.02.018

2008

2008
Epileptic and developmental disorders of the speech cortex: ligand/receptor interaction of wild-type and mutant SRPX2 with the plasminogen activator receptor uPAR

Patrice Roll, Barbara Royer-Zemmour, Magali Ponsole-Lenfant, Hyam Gara, Christian Lévêque, Annick Massacrier, Géraldine Ferracci, Jennifer Cillario, Andrée Robaglia-Schlupp, Renaud Vincentelli, Pierre Cau, Pierre Szepetowski

Human Molecular Genetics 17:3617-3630 (2008)10.1093/hmg/ddn256

2004

2004
Structure of Escherichia coli YhdH, a putative quinone oxidoreductase

Gerlind Sulzenbacher, Véronique Roig-Zamboni, Fabienne Pagot, Sacha Grisel, Aurelia Salomoni, Christel Valencia, Valérie Campanacci, Renaud Vincentelli, Mariella Tegoni, Hans Eklund, Christian Cambillau

Acta crystallographica Section D : Structural biology [1993-...] (2004)10.1107/S0907444904020220

2004
The Escherichia coli YadB Gene Product Reveals a Novel Aminoacyl-tRNA Synthetase Like Activity

Valérie Campanacci, Daniel Y. Dubois, Hubert Becker, Daniel Kern, Silvia Spinelli, Christel Valencia, Fabienne Pagot, Aurelia Salomoni, Sacha Grisel, Renaud Vincentelli, Christophe Bignon, Jacques Lapointe, Richard Giege, Christian Cambillau

Journal of Molecular Biology 337:273-283 (2004)10.1016/j.jmb.2004.01.027

2003

2003
Medium-Scale Structural Genomics: Strategies for Protein Expression and Crystallization

Renaud Vincentelli, Christophe Bignon, Arnaud Gruez, Stéphane Canaan, Gerlind Sulzenbacher, Mariella Tegoni, Rie Campanacci, Christian Cambillau

Accounts of Chemical Research (2003)10.1021/ar010130s

2002

2002
A medium-throughput crystallization approach

Gerlind Sulzenbacher, Arnaud Gruez, Véronique Roig-Zamboni, Silvia Spinelli, Christel Valencia, Fabienne Pagot, Renaud Vincentelli, Christophe Bignon, Aurelia Salomoni, Sacha Grisel, Damien Maurin, Céline Huyghe, Kent Johansson, Alice Grassick, Alain Roussel, Yves Bourne, Sophie Perrier, Linda Miallau, Phillippe Cantau, Eric Blanc, Michel Genevois, Alain Grossi, André Zenatti, Valérie Campanacci, Christian Cambillau

Acta crystallographica Section D : Structural biology [1993-...] 58:2109-2115 (2002)10.1107/s0907444902013938