Yves Bourne est directeur de recherche au CNRS. Aprés un doctorat en 1990 à l’Université Aix-Marseille III intitulé “Etude cristallographique de l’interaction entre une lectine végétale et divers saccharides” sous la direction de C Cambillau, il a intégré le laboratoire LCCMB (ensuite renommé laboratoire AFMB) en 1991 en tant que chargé de recherche CNRS. En 1994, il rejoint l’équipe du Pr John A. Tainer au Scripps Research Institute à La Jolla (CA, USA) pour travailler sur des protéines régulatrices du cycle cellulaire lors d’un séjour sabbatique de 2 ans. Depuis 2003, il est responsabble, et depuis 2012 co-responsable avec Pascale Marchot, de l’équipe « Glycobiologie et Neurobiologie Structurales » du laboratoire AFMB. Depuis 2008, il est directeur du laboratoire AFMB (CNRS / Aix-Marseille Université) après en avoir été le directeur-adjoint en 2007.

Il s’intéresse à élucider l’architecture, principalement par cristallographie aux rayons X, et le mode d’action de protéines ou d’enzymes agissant sur les glycanes mais aussi de protéines synaptiques et des complexes qu’elles peuvent former avec d’autres partenaires protéiques ou glycoprotéines. Les résultats de ces travaux ont un fort intérêt biomédical et permettent d’appréhender les bases moléculaires de certains désordres de la glycosylation des protéines, certaines infections pulmonaires ou des troubles autistiques.

Il est co-auteur d’une centaine d’articles dans des revues à comité de lecture et a contribué à plus d’une centaine de structures déposées dans la “Protein Data Bank”.

Publications

2022

2022
Molecular basis for substrate recognition and septum cleavage by AtlA, the major N-acetylglucosaminidase of Enterococcus faecalis

Véronique Roig-Zamboni, Sarah Barelier, Robert Dixon, Nicola Galley, Amani Ghanem, Quoc Phong Nguyen, Héloize Cahuzac, Bartłomiej Salamaga, Peter Davis, Yves Bourne, Stéphane Mesnage, Florence Vincent

Journal of Biological Chemistry 101915 (2022)10.1016/j.jbc.2022.101915

2021

2021
Comparative mapping of selected structural determinants on the extracellular domains of cholinesterase-like cell-adhesion molecules.

Davide Comoletti, Laura Trobiani, Arnaud Chatonnet, Yves Bourne, P. Marchot

Neuropharmacology 184:108381 (2021)10.1016/j.neuropharm.2020.108381

2020

2020
The neuroligins and the synaptic pathway in Autism Spectrum Disorder.

Laura Trobiani, Maria Meringolo, Tamara Diamanti, Yves Bourne, P. Marchot, Giuseppina Martella, Luciana Dini, Antonio Pisani, Antonella de Jaco, Paola Bonsi

Neuroscience and Biobehavioral Reviews 119:37-51 (2020)10.1016/j.neubiorev.2020.09.017

2020
Biochemical characterization of a glycosyltransferase Gtf3 from Mycobacterium smegmatis: a case study of improved protein solubilization

Mahfoud Bakli, Loukmane Karim, Nassima Mokhtari-Soulimane, Hafida Merzouk, Florence Vincent

3 Biotech 10 (2020)10.1007/s13205-020-02431-x

2020
The Ig-like domain of Punctin/MADD-4 is the primary determinant for interaction with the ectodomain of neuroligin NLG-1

Semeli Platsaki, Xin Zhou, Bérangère Pinan-Lucarré, Vincent Delauzun, Haijun Tu, Pascal Mansuelle, Patrick Fourquet, Yves Bourne, Jean-Louis Bessereau, P. Marchot

Journal of Biological Chemistry 16267-16279. (2020)10.1074/jbc.RA120.014591

2019

2019
Substrate binding mode and catalytic mechanism of human heparan sulfate d-glucuronyl C5 epimerase

Claire Debarnot, Yoan Monneau, Veronique Roig-Zamboni, Vincent Delauzun, Christine Le Narvor, Emeline Richard, Jérôme Hénault, Adeline Goulet, Firas Fadel, Romain R Vivès, Bernard Priem, David Bonnaffé, Hugues Lortat-Jacob, Yves Bourne

Proceedings of the National Academy of Sciences of the United States of America 116:6760-6765 (2019)10.1073/pnas.1818333116

2019
Overview of the Structure–Function Relationships of Mannose-Specific Lectins from Plants, Algae and Fungi

Annick Barre, Yves Bourne, Els van Damme, Pierre Rougé

International Journal of Molecular Sciences 20:254 (2019)10.3390/ijms20020254

2019
An evolutionary perspective on the first disulfide bond in members of the cholinesterase-carboxylesterase (COesterase) family: Possible outcomes for cholinesterase expression in prokaryotes

Arnaud Chatonnet, Xavier Brazzolotto, Thierry Hotelier, Nicolas Lenfant, P. Marchot, Yves Bourne

Chemico-Biological Interactions 308:179-184 (2019)10.1016/j.cbi.2019.05.016

2018

2018
Genetic and enzymatic characterization of 3-O-sulfotransferase SNPs associated with Plasmodium falciparum parasitaemia

Ngoc Thy Nguyen, Romain R Vivès, Magali Iché-Torres, Vincent Delauzun, Els Saesen, Veronique Roig-Zamboni, Hugues Lortat-Jacob, Pascal Rihet, Yves Bourne

Glycobiology 28:534-541 (2018)10.1093/glycob/cwy038

2018
Glycosylate and move! The glycosyltransferase Maf is involved in bacterial flagella formation

Gerlind Sulzenbacher, Veronique Roig-Zamboni, Régine Lebrun, Yann Guérardel, Dorothée Murat, Pascal Mansuelle, Nao Yamakawa, Xin-Xin Qian, Renaud Vincentelli, Yves Bourne, Long-Fei Wu, François Alberto

Environmental Microbiology 20:228-240 (2018)10.1111/1462-2920.13975

2017

2017
Structural and functional insights into the periplasmic detector domain of the GacS histidine kinase controlling biofilm formation in Pseudomonas aeruginosa

Ahmad Ali-Ahmad, Firas Fadel, Corinne Sebban-Kreuzer, Moly Ba, Gauthier Dangla Pélissier, Olivier Bornet, Françoise Guerlesquin, Yves Bourne, Christophe Bordi, Florence Vincent

Scientific Reports 7:11262 (2017)10.1038/s41598-017-11361-3

2017
Structure of human lysosomal acid α-glucosidase-a guide for the treatment of Pompe disease

Véronique Roig-Zamboni, Beatrice Cobucci-Ponzano, Roberta Iacono, Maria Ferrara, Stanley Germany, Yves Bourne, Giancarlo Parenti, Marco Moracci, Gerlind Sulzenbacher

Nature Communications (2017)10.1038/s41467-017-01263-3

2017
Cyclic imine toxins from dinoflagellates: a growing family of potent antagonists of the nicotinic acetylcholine receptors

Jordi Molgó, P. Marchot, Rómulo Aráoz, Evelyne Benoit, Bogdan I Iorga, Armen Zakarian, Palmer Taylor, Yves Bourne, Denis Servent

Journal of Neurochemistry 142:41-51 (2017)10.1111/jnc.13995

2017
Structure of human lysosomal acid α-glucosidase–a guide for the treatment of Pompe disease

Veronique Roig-Zamboni, Beatrice Cobucci-Ponzano, Roberta Iacono, Maria Carmina Ferrara, Stanley Germany, Yves Bourne, Giancarlo Parenti, Marco Moracci, Gerlind Sulzenbacher

Nature Communications 8:1111 (2017)10.1038/s41467-017-01263-3

2017
Structural insights into a family 39 glycoside hydrolase from the gut symbiont Bacteroides cellulosilyticus WH2

Ahmad Ali-Ahmad, Marie-Line Garron, Véronique Zamboni, Nicolas Lenfant, Didier Nurizzo, Bernard Henrissat, Jean-Guy Berrin, Yves Bourne, Florence Vincent

Journal of Structural Biology 197:227-235 (2017)10.1016/j.jsb.2016.11.004

2017
NMR assignments of the GacS histidine-kinase periplasmic detection domain from Pseudomonas aeruginosa PAO1

Ahmad Ali-Ahmad, Olivier Bornet, Firas Fadel, Yves Bourne, Florence Vincent, Christophe Bordi, Françoise Guerlesquin, Corinne Sebban-Kreuzer

Biomolecular NMR Assignments 11:25-28 (2017)10.1007/s12104-016-9714-7

2016

2016
Relationships of human alpha/beta hydrolase fold proteins and other organophosphate-interacting proteins

Nicolas Lenfant, Yves Bourne, P. Marchot, Arnaud Chatonnet

Chemico-Biological Interactions 259:343-351 (2016)10.1016/j.cbi.2016.04.027

2016
Steric and Dynamic Parameters Influencing In Situ Cycloadditions to Form Triazole Inhibitors with Crystalline Acetylcholinesterase

Yves Bourne, K. Barry Sharpless, Palmer Taylor, P. Marchot

Journal of the American Chemical Society 138:1611-1621 (2016)10.1021/jacs.5b11384

2015

2015
2015
Marine Macrocyclic Imines, Pinnatoxins A and G: Structural Determinants and Functional Properties to Distinguish Neuronal α7 from Muscle α12βγδ nAChRs.

Yves Bourne, Gerlind Sulzenbacher, Zoran Radić, Rómulo Aráoz, Morgane Reynaud, Evelyne Benoit, Armen Zakarian, Denis Servent, Jordi Molgó, Palmer Taylor, P. Marchot

Structure (London, England : 1993) 23:1106-15 (2015)10.1016/j.str.2015.04.009

2015
Structural basis for carbohydrate binding properties of a plant chitinase-like agglutinin with conserved catalytic machinery

Gerlind Sulzenbacher, Veronique Roig-Zamboni, Willy J. Peumans, Bernard Henrissat, Els J. M. Damme, Yves Bourne

Journal of Structural Biology 190:115-121 (2015)10.1016/j.jsb.2015.01.013

2014

2014
The neuroligins and their neurexin ligands: from structure to function at the synapse

Yves Bourne, P. Marchot

Journal of Molecular Neuroscience 53:387-396 (2014)10.1007/s12031-014-0234-6

2014
Structural and Functional Characterization of the Clostridium perfringens N-Acetylmannosamine-6-phosphate 2-Epimerase Essential for the Sialic Acid Salvage Pathway

Marie-Cécile Pélissier, Corinne Sebban-Kreuzer, Françoise Guerlesquin, James Brannigan, Yves Bourne, Florence Vincent

Journal of Biological Chemistry 289:35215-35224 (2014)10.1074/jbc.m114.604272

2014
Tracking the origin and divergence of cholinesterases and neuroligins: the evolution of synaptic proteins

Nicolas Lenfant, Thierry Hotelier, Yves Bourne, P. Marchot, Arnaud Chatonnet

Journal of Molecular Neuroscience 53:362-369 (2014)10.1007/s12031-013-0194-2

2014
Structural and biochemical characterization of the β-N-acetylglucosaminidase from Thermotoga maritima: toward rationalization of mechanistic knowledge in the GH73 family

Alexandra Lipski, Mireille Hervé, Vincent Lombard, Didier Nurizzo, Dominique Mengin-Lecreulx, Yves Bourne, Florence Vincent

Glycobiology 25:319-330 (2014)10.1093/glycob/cwu113

2013

2013
Molecular Characterization of Monoclonal Antibodies that Inhibit Acetylcholinesterase by Targeting the Peripheral Site and Backdoor Region

Yves Bourne, Ludovic Renault, Sosthène Essono, Grégoire Mondielli, Patricia Lamourette, Didier Boquet, Jacques Grassi, P. Marchot

PLoS ONE 8:e77226 (2013)10.1371/journal.pone.0077226

2013
Proteins with an alpha/beta hydrolase fold: Relationships between subfamilies in an ever-growing superfamily.

Nicolas Lenfant, Thierry Hotelier, Yves Bourne, P. Marchot, Arnaud Chatonnet

Chemico-Biological Interactions 203:266-268 (2013)10.1016/j.cbi.2012.09.003

2013
ESTHER, the database of the α/β-hydrolase fold superfamily of proteins: tools to explore diversity of functions

Nicolas Lenfant, Thierry Hotelier, Eric Velluet, Yves Bourne, P. Marchot, Arnaud Chatonnet

Nucleic Acids Research 41:423-429 (2013)10.1093/nar/gks1154

2012

2012
The Structure of Human DNase I Bound to Magnesium and Phosphate Ions Points to a Catalytic Mechanism Common to Members of the DNase I-like Superfamily

Goetz Parsiegla, Christophe Noguere, Lydia Santell, Robert Lazarus, Yves Bourne

Biochemistry 51:10250-10258 (2012)10.1021/bi300873f

2012
Structural Insights into Antibody Sequestering and Neutralizing of Na+ Channel α-Type Modulator from Old World Scorpion Venom.

Igor P Fabrichny, Grégoire Mondielli, Sandrine Conrod, Marie-France Martin-Eauclaire, Yves Bourne, P. Marchot

Journal of Biological Chemistry 287:14136-48 (2012)10.1074/jbc.M111.315382

2010

2010
Conformational remodeling of femtomolar inhibitor-acetylcholinesterase complexes in the crystalline state.

Yves Bourne, Zoran Radić, Palmer Taylor, P. Marchot

Journal of the American Chemical Society 132:18292-300 (2010)10.1021/ja106820e

2010
Crystal structures of a family 8 polysaccharide lyase reveal open and highly occluded substrate-binding cleft conformations

Zainab Elmabrouk, Florence Vincent, Meng Zhang, Nicola Smith, Johan Turkenburg, Simon Charnock, Gary Black, Edward Taylor

Proteins - Structure, Function and Bioinformatics 79:965-974 (2010)10.1002/prot.22938

2010
Structural insights into the exquisite selectivity of neurexin/neuroligin synaptic interactions.

Philippe Leone, Davide Comoletti, Géraldine Ferracci, Sandrine Conrod, Simon U Garcia, Palmer Taylor, Yves Bourne, P. Marchot

EMBO Journal 29:2461-71 (2010)10.1038/emboj.2010.123

2010
Structure of a polyisoprenoid binding domain from Saccharophagus degradans implicated in plant cell wall breakdown

Florence Vincent, David Dal Molin, Ronald Weiner, Yves Bourne, Bernard Henrissat

FEBS Letters 584:1577-1584 (2010)10.1016/j.febslet.2010.03.015

2010
Structural determinants in phycotoxins and AChBP conferring high affinity binding and nicotinic AChR antagonism

Yves Bourne, Zoran Radić, Rómulo Aráoz, Todd Talley, Evelyne Benoit, Denis Servent, Palmer Taylor, Jordi Molgó, Pascale Marchot

Proceedings of the National Academy of Sciences of the United States of America 107:6076-6081 (2010)10.1073/pnas.0912372107

2010
Structural determinants in phycotoxins and AChBP conferring high affinity binding and nicotinic AChR antagonism.

Yves Bourne, Zoran Radic, Rómulo Aráoz, Todd T Talley, Evelyne Benoit, Denis Servent, Palmer Taylor, Jordi Molgó, P. Marchot

Proceedings of the National Academy of Sciences of the United States of America epub ahead of print (2010)10.1073/pnas.0912372107

2010
Structure-function relationships of the alpha/beta-hydrolase fold domain of neuroligin: A comparison with acetylcholinesterase.

Philippe Leone, Davide Comoletti, Palmer Taylor, Yves Bourne, P. Marchot

Chem Biol Interact epub ahead of print (2010)10.1016/j.cbi.2010.01.030

2010
Crystal Structure of the GalNAc/Gal-Specific Agglutinin from the Phytopathogenic Ascomycete Sclerotinia sclerotiorum Reveals Novel Adaptation of a β-Trefoil Domain

Gerlind Sulzenbacher, Véronique Roig-Zamboni, Willy Peumans, Pierre Rougé, Els van Damme, Yves Bourne

Journal of Molecular Biology 400:715-723 (2010)10.1016/j.jmb.2010.05.038

2010
A novel α-N-acetylgalactosaminidase family with an NAD+-dependent catalytic mechanism suitable for enzymatic removal of blood group A antigens

Gerlind Sulzenbacher, Qiyong Liu, Eric Bennett, Steven Levery, Yves Bourne, Guillaume Ponchel, Henrik Clausen, Bernard Henrissat

Biocatalysis and Biotransformation 28:22-32 (2010)10.3109/10242420903424259

2009

2009
Interaction between the SifA Virulence Factor and Its Host Target SKIP Is Essential for Salmonella Pathogenesis

Lautaro Diacovich, Audrey Dumont, Daniel Lafitte, Elodie Soprano, Aude-Agnès Guilhon, Christophe Bignon, Jean-Pierre Gorvel, Yves Bourne, Stéphane Méresse

Journal of Biological Chemistry 284:33151-33160 (2009)10.1074/jbc.m109.034975

2009
Interaction between the sifa virulence factor and its host target skip is essential for salmonella pathogenesis.

Lautaro Diacovich, Audrey Dumont, Daniel Lafitte, Elodie Soprano, Aude-Agnes Guilhon, Christophe Bignon, Jean-Pierre Gorvel, Yves Bourne, Stephane Meresse

Journal of Biological Chemistry epub ahead of print (2009)10.1074/jbc.M109.034975

2009
Structural determinants for interaction of partial agonists with acetylcholine binding protein and neuronal α7 nicotinic acetylcholine receptor

Ryan Hibbs, Gerlind Sulzenbacher, Jianxin Shi, Todd Talley, Sandrine Conrod, William Kem, Palmer Taylor, P. Marchot, Yves Bourne

EMBO Journal 28:3040-3051 (2009)10.1038/emboj.2009.227

2009
Distinct oligomeric forms of the Pseudomonas aeruginosa RetS sensor domain modulate accessibility to the ligand binding site

Florence Vincent, Adam Round, Aline Reynaud, Christophe Bordi, Alain Filloux, Yves Bourne

Environmental Microbiology 12:1775-1786 (2009)10.1111/j.1462-2920.2010.02264.x

2008

2008
Glycosyltransferases, glycoside hydrolases: surprise, surprise!

Bernard Henrissat, Gerlind Sulzenbacher, Yves Bourne

Current Opinion in Structural Biology (2008)10.1016/j.sbi.2008.09.003

2008
Glycosyltransferases, glycoside hydrolases: surprise, surprise!

Bernard Henrissat, Gerlind Sulzenbacher, Yves Bourne

Current Opinion in Structural Biology 18:527-533 (2008)10.1016/j.sbi.2008.09.003

2007

2007
Structural Analysis of the Synaptic Protein Neuroligin and Its β-Neurexin Complex: Determinants for Folding and Cell Adhesion

Igor Fabrichny, Philippe Leone, Gerlind Sulzenbacher, Davide Comoletti, Meghan Miller, Palmer Taylor, Yves Bourne, P. Marchot

Neuron 56:979-991 (2007)10.1016/j.neuron.2007.11.013

2007
Des glycosidases bactériennes pour du sang universel

Gerlind Sulzenbacher, Yves Bourne, Bernard Henrissat

médecine/sciences (2007)10.1051/medsci/20072389703

2007
Bacterial glycosidases for the production of universal red blood cells

Qiyong Liu, Gerlind Sulzenbacher, Huaiping Yuan, Eric Bennett, Greg Pietz, Kristen Saunders, Jean Spence, Edward Nudelman, Steven Levery, Thayer White, John Neveu, William Lane, Yves Bourne, Martin Olsson, Bernard Henrissat, Henrik Clausen

Nature Biotechnology (2007)10.1038/nbt1298

2007
Gene overexpression and biochemical characterization of the biotechnologically relevant chlorogenic acid hydrolase from Aspergillus niger

Isabelle Benoit, Michèle Asther, Yves Bourne, David Navarro, Stéphane Canaan, Laurence Lesage-Meessen, Marga Herweijer, Pedro Coutinho, Marcel Asther, Eric Record

Applied and Environmental Microbiology 73:5624-5632 (2007)10.1128/AEM.00374-07

2006

2006
Substrate and product trafficking through the active center gorge of acetylcholinesterase analyzed by crystallography and equilibrium binding.

Yves Bourne, Zoran Radic, Gerlind Sulzenbacher, Esther Kim, Palmer Taylor, P. Marchot

Journal of Biological Chemistry 281:29256-67 (2006)10.1074/jbc.M603018200

2006
Functional characterization of the glycosyltransferase domain of penicillin-binding protein 1a from Thermotoga maritima

Julien Offant, François Michoux, Annabelle Dermiaux, Jacques Biton, Yves Bourne

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1764:1036-1042 (2006)10.1016/j.bbapap.2006.03.012

2006
LppX is a lipoprotein required for the translocation of phthiocerol dimycocerosates to the surface of Mycobacterium tuberculosis

Gerlind Sulzenbacher, Stéphane Canaan, Yann Bordat, Olivier Neyrolles, Gustavo Stadthagen, Véronique Roig-Zamboni, Jean Rauzier, Damien Maurin, Françoise Laval, Mamadou Daffé, Christian Cambillau, Brigitte Gicquel, Yves Bourne, Mary Jackson

EMBO Journal (2006)10.1038/sj.emboj.7601048

2006
Structure and Activity of Two Metal Ion-dependent Acetylxylan Esterases Involved in Plant Cell Wall Degradation Reveals a Close Similarity to Peptidoglycan Deacetylases

Edward Taylor, Tracey Gloster, Johan Turkenburg, Florence Vincent, A. Marek Brzozowski, Claude Dupont, François Shareck, Maria S.J. Centeno, José A.M. Prates, Vladimír Puchart, Luís M.A. Ferreira, Carlos M.G.A. Fontes, Peter Biely, Gideon Davies

Journal of Biological Chemistry 281:10968-10975 (2006)10.1074/jbc.M513066200

2006
Structural characterization of agonist and antagonist-bound acetylcholine-binding protein from Aplysia californica.

Scott B Hansen, Gerlind Sulzenbacher, Tom Huxford, P. Marchot, Yves Bourne, Palmer Taylor

Journal of Molecular Neuroscience 30:101-2 (2006)

2006
Structural comparison of three crystalline complexes of a peptidic toxin with a synaptic acetylcholine recognition protein.

Yves Bourne, Scott B Hansen, Gerlind Sulzenbacher, Todd T Talley, Tom Huxford, Palmer Taylor, P. Marchot

Journal of Molecular Neuroscience 30:103-4 (2006)

2005

2005
Structural insights into AChE inhibition by monoclonal antibodies

Ludovic Renault, Sosthène Essono, Marianick Juin, Didier Boquet, Jacques Grassi, Yves Bourne, P. Marchot

Chemico-Biological Interactions 157-158:397-400 (2005)10.1016/j.cbi.2005.10.073

2005
A. niger protein “EstA”, perhaps a new electrotactin, defines a new class of fungal esterases within the α/β hydrolase fold superfamily

Yves Bourne, Alinda Hasper, Henri Chahinian, Ludovic Renault, Marianick Juin, Leo de Graaff, P. Marchot

Chemico-Biological Interactions 157-158:395-396 (2005)10.1016/j.cbi.2005.10.072

2005
Structure and Kinetics of a Monomeric Glucosamine 6-Phosphate Deaminase

Florence Vincent, Gideon Davies, James Brannigan

Journal of Biological Chemistry 280:19649-19655 (2005)10.1074/jbc.m502131200

2005
Crystal structure of the conserved hypothetical protein Rv1155 from Mycobacterium tuberculosis

Stéphane Canaan, Gerlind Sulzenbacher, Véronique Roig-Zamboni, Loréna Scappuccini-Calvo, Frédéric Frassinetti, Damien Maurin, Christian Cambillau, Yves Bourne, Christian Griesinger

FEBS Letters (2005)10.1016/j.febslet.2004.11.069

2005
Crystal structure of a Cbtx-AChBP complex reveals essential interactions between snake alpha-neurotoxins and nicotinic receptors,

Yves Bourne, T. T. Talley, Hansen S. B., P. Taylor, P. Marchot

EMBO Reports 24:1512-1522 (2005)

2005
Structures of Aplysia AChBP complexes with nicotinic agonists and antagonists reveal distinctive binding interfaces and conformations

Scott Hansen, Gerlind Sulzenbacher, Tom Huxford, P. Marchot, Palmer Taylor, Yves Bourne

EMBO Journal 24:3635-3646 (2005)10.1038/sj.emboj.7600828

2005
Structural insights into conformational flexibility at the peripheral site and within the active center gorge of AChE, Chem Biol Interact. 157-158, 159-65.

Yves Bourne, Z. Radic, Kolb H. C., Sharpless K. B., P. Taylor, P. Marchot

Chemico-Biological Interactions 157-158:159-65. (2005)

2005
Structures of Aplysia AChBP complexes with nicotinic agonists and antagonists reveal distinctive binding interfaces and conformations

Hansen S. B., G. Sulzenbacher, T. Huxford, P. Marchot, P. Taylor, Yves Bourne

EMBO Reports 24:3635-46 (2005)

2004

2004
The Use of Forced Protein Evolution to Investigate and Improve Stability of Family 10 Xylanases

Simon Andrews, Edward Taylor, Gavin Pell, Florence Vincent, Valérie M.-A. Ducros, Gideon Davies, Jeremy Lakey, Harry Gilbert

Journal of Biological Chemistry 279:54369-54379 (2004)10.1074/jbc.M409044200

2004
Common Inhibition of Both β-Glucosidases and β-Mannosidases by Isofagomine Lactam Reflects Different Conformational Itineraries for Pyranoside Hydrolysis

Florence Vincent, Tracey Gloster, James Macdonald, Carl Morland, Robert Stick, Fernando Dias, José Prates, Carlos Fontes, Harry Gilbert, Gideon Davies

ChemBioChem 5:1596-1599 (2004)10.1002/cbic.200400169

2004
Crystal structures of bovine odorant-binding protein in complex with odorant molecules

Florence Vincent, Roberto Ramoni, Silvia Spinelli, Stefano Grolli, Mariella Tegoni, Christian Cambillau

European Journal of Biochemistry 271:3832-3842 (2004)10.1111/j.1432-1033.2004.04315.x

2004
Insights into the Molecular Determinants of Substrate Specificity in Glycoside Hydrolase Family 5 Revealed by the Crystal Structure and Kinetics of Cellvibrio mixtus Mannosidase 5A

Fernando Dias, Florence Vincent, Gavin Pell, José Prates, Maria Centeno, Louise Tailford, Luís Ferreira, Carlos Fontes, Gideon Davies, Harry Gilbert

Journal of Biological Chemistry 279:25517-25526 (2004)10.1074/jbc.m401647200

2004
Aspergillus niger Protein EstA Defines a New Class of Fungal Esterases within the α/β Hydrolase Fold Superfamily of Proteins

Yves Bourne, Alinda Hasper, Henri Chahinian, Marianick Juin, Leo de Graaff, P. Marchot

Structure 12:677-687 (2004)10.1016/j.str.2004.03.005

2004
Crystal Structure of Thermotoga maritima ␣-L-Fucosidase INSIGHTS INTO THE CATALYTIC MECHANISM AND THE MOLECULAR BASIS FOR FUCOSIDOSIS

Gerlind Sulzenbacher, Christophe Bignon, Takeshi Nishimura, Chris Tarling, Stephen Withers, Bernard Henrissat, Yves Bourne

Journal of Biological Chemistry (2004)10.1074/jbc.M313783200

2004
Crystal Structure of E. coli YddE Protein Reveals a Striking Homology With Diaminopimelate Epimerase

Alice Grassick, Gerlind Sulzenbacher, Véronique Roig-Zamboni, Valérie Campanacci, Christian Cambillau, Yves Bourne

Proteins - Structure, Function and Bioinformatics 55:764-767 (2004)10.1002/prot.20025

2003

2003
Identification of the Catalytic Nucleophile of the Family 29 α-L-Fucosidase from Thermotoga maritima through Trapping of a Covalent Glycosyl-Enzyme Intermediate and Mutagenesis

Chris Tarling, Shouming He, Gerlind Sulzenbacher, Christophe Bignon, Yves Bourne, Bernard Henrissat, Stephen Withers

Journal of Biological Chemistry (2003)10.1074/jbc.M306610200

2003
The Three-dimensional Structure of the N-Acetylglucosamine-6-phosphate Deacetylase, NagA, from Bacillus subtilis

Florence Vincent, David Yates, Elspeth Garman, Gideon Davies, James Brannigan

Journal of Biological Chemistry 279:2809-2816 (2003)10.1074/jbc.m310165200

2003
Multifunctional Xylooligosaccharide/Cephalosporin C Deacetylase Revealed by the Hexameric Structure of the Bacillus subtilis Enzyme at 1.9Å Resolution

Florence Vincent, Simon Charnock, Koen H.G. Verschueren, Johan Turkenburg, David Scott, Wendy Offen, Shirley Roberts, Gavin Pell, Harry Gilbert, Gideon Davies, James Brannigan

Journal of Molecular Biology 330:593-606 (2003)10.1016/s0022-2836(03)00632-6

2003
Structural insights into ligand interactions at the acetylcholinesterase peripheral anionic site

Yves Bourne, Palmer Taylor, Zoran Radic, P. Marchot

EMBO Journal 22:1-12 (2003)10.1093/emboj/cdg005

2002

2002
Boar salivary lipocalin

Silvia Spinelli, Florence Vincent, Paolo Pelosi, Mariella Tegoni, Christian Cambillau

European Journal of Biochemistry 269:2449-2456 (2002)10.1046/j.1432-1033.2002.02901.x

2002
Mechanism of Acetylcholinesterase Inhibition by Fasciculin: A 5-ns Molecular Dynamics Simulation

Kaihsu Tai, Tongye Shen, Richard Henchman, Yves Bourne, P. Marchot, J. Andrew Mccammon

Journal of the American Chemical Society 124:6153-6161 (2002)10.1021/ja017310h

2002
Control of domain swapping in bovine odorant-binding protein

Roberto Ramoni, Florence Vincent, Alison Ashcroft, Paolo Accornero, Stefano Grolli, Christel Valencia, Mariella Tegoni, Christian Cambillau

Biochemical Journal (2002)

2002
A medium-throughput crystallization approach

Gerlind Sulzenbacher, Arnaud Gruez, Véronique Roig-Zamboni, Silvia Spinelli, Christel Valencia, Fabienne Pagot, Renaud Vincentelli, Christophe Bignon, Aurelia Salomoni, Sacha Grisel, Damien Maurin, Céline Huyghe, Kent Johansson, Alice Grassick, Alain Roussel, Yves Bourne, Sophie Perrier, Linda Miallau, Phillippe Cantau, Eric Blanc, Michel Genevois, Alain Grossi, André Zenatti, Valérie Campanacci, Christian Cambillau

Acta crystallographica Section D : Structural biology [1993-...] 58:2109-2115 (2002)10.1107/s0907444902013938

2001

2001
Crystal Structure of Streptococcus pneumoniae N -Acetylglucosamine-1-phosphate Uridyltransferase Bound to Acetyl-coenzyme A Reveals a Novel Active Site Architecture

Gerlind Sulzenbacher, Laurent Gal, Caroline Peneff, Florence Fassy, Yves Bourne

Journal of Biological Chemistry 276:11844-11851 (2001)10.1074/jbc.M011225200

2001
Crystal structure of aphrodisin, a sex pheromone from female hamster11Edited by R Huber

Florence Vincent, Dietrich Löbel, Kieron Brown, Silvia Spinelli, Phillip Grote, Heinz Breer, Christian Cambillau, Mariella Tegoni

Journal of Molecular Biology 305:459-469 (2001)10.1006/jmbi.2000.4241

2001
The insect attractant 1-Octen-3-o1 is the natural ligand of bovine odorant-binding protein

Roberto Ramoni, Florence Vincent, Stefano Grolli, Virna Conti, Christian Malosse, Francois Boyer, Patricia Nagnan-Le Meillour, Silvia Spinelli, Christian Cambillau, Mariella Tegoni

Journal of Biological Chemistry 276:7150-7155 (2001)10.1074/jbc.M010368200

2000

2000
Immunocytochemical Localization and Crystal Structure of Human Frequenin (Neuronal Calcium Sensor 1)

Yves Bourne, Jens Dannenberg, Verena Pollmann, P. Marchot, Olaf Pongs

Journal of Biological Chemistry 276:11949-11955 (2000)10.1074/jbc.m009373200

2000
Mammalian odorant binding proteins

Mariella Tegoni, Paolo Pelosi, Florence Vincent, Silvia Spinelli, Valérie Campanacci, Stefano Grolli, Roberto Ramoni, Christian Cambillau

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 1482:229-240 (2000)10.1016/s0167-4838(00)00167-9

2000
Complexes of porcine odorant binding protein with odorant molecules belonging to different chemical classes

Florence Vincent, Silvia Spinelli, Roberto Ramoni, Stefano Grolli, Paolo Pelosi, Christian Cambillau, Mariella Tegoni

Journal of Molecular Biology 300:127-139 (2000)10.1006/jmbi.2000.3820

1999

1999
Conformational Flexibility of the Acetylcholinesterase Tetramer Suggested by X-ray Crystallography*

Yves Bourne, Jacques Grassi, Pierre Bougis, P. Marchot

Journal of Biological Chemistry 274:30370-30376 (1999)

1999
Crystal Structure of Mouse Acetylcholinesterase

Yves Bourne, Palmer Taylor, Pierre Bougis, P. Marchot

Journal of Biological Chemistry 274:2963-2970 (1999)10.1074/jbc.274.5.2963

1998

1998
Inhibition of mouse acetylcholinesterase by fasciculin: Crystal structure of the complex and mutagenesis of fasciculin

P. Marchot, Yves Bourne, Claudine Prowse, Pierre Bougis, Palmer Taylor

Toxicon 36:1613-1622 (1998)10.1016/s0041-0101(98)00154-8

1998
Barley alpha-amylase bound to its endogenous protein inhibitor BASI: crystal structure of the complex at 1.9 A resolution.

F. Vallee, A. Kadziola, Yves Bourne, M. Juy, Kw Rodenburg, B. Svensson, R. Haser

Structure 6:649-659 (1998)

1996

1996
Soluble monomeric acetylcholinesterase from mouse: Expression, purification, and crystallization in complex with fasciculin

P. Marchot, Raimond B.G. Ravelli, Mia Raves, Yves Bourne, Daniel Vellom, Joan Kanter, Shelley Camp, Joel Sussman, Palmer Taylor

Protein Science 5:672-679 (1996)10.1002/pro.5560050411

1995

1994

1994
Structures of a legume lectin complexed with the human lactotransferrin N2 fragment, and with an isolated biantennary glycopeptide: role of the fucose moiety.

Yves Bourne, J. Mazurier, Delphine Legrand, Pierre Rougé, J. Montreuil, G. Spik, C. Cambillau

Structure / Struct Fold Des; Structure (Camb ) 2:209-19 (1994)

1994
Primary and three-dimensional structure of lactotransferrin (lactoferrin) glycans.

G. Spik, B. Coddeville, J. Mazurier, Yves Bourne, C. Cambillaut, J. Montreuil

Advances in Experimental Medicine and Biology 357:21-32 (1994)

1992

1992
Crystallization and preliminary X-ray diffraction study of Lathyrus ochrus isolectin II complexed to the human lactotransferrin N2 fragment.

Yves Bourne, M. P. Nésa, Pierre Rougé, J. Mazurier, Delphine Legrand, G. Spik, J. Montreuil, C. Cambillau

Journal of Molecular Biology 227:938-41 (1992)

1989

1989
Crystallization and preliminary X-ray study of horse pancreatic lipase

Dominique Lombardo, Catherine Chapus, Yves Bourne, Christian Cambillau

Journal of Molecular Biology 205:259-261 (1989)10.1016/0022-2836(89)90380-x

1988

1988
Crystallization and preliminary X-ray studies of two isolectins from the seeds of Lathyrus ochrus

Yves Bourne, Pierre Rougé, Christian Cambillau

Journal of Molecular Biology 202:685-687 (1988)10.1016/0022-2836(88)90299-9