Techniques biophysiques
Le service « techniques biophysiques » offre un ensemble d’équipements de pointe pour la caractérisation de complexes moléculaires et le criblage ou l’analyse d’interactions moléculaires, en solution ou sur support solide.
Ces outils sont particulièrement adaptés à l’étude des complexes de type :
- protéine/protéine
- protéine/acide nucléique
- protéine/petite molécule
- protéine/détergent
Accessibilité
Académiques et industriels
Coût
A définir en fonction du projet.
Comment faire une demande ?
- Compléter le formulaire
en cliquant “Marseille” à partir de l’item “Biophysical facility” - Contact AFMB : Maria Mate
Equipement spécifique
- Titration calorimétrique isotherme (ITC) : MicroCal iTC200 (Malvern Panalytical)
- Thermophorèse à micro échelle (MST) : Monolith NT.115 (NanoTemper Technologies GmbH)
- Gel filtration analytique/diffusion statique de la lumière multi angle/ réfractométrie (SEC-MALS) :
- HPLC Ultimate 3000 (Thermo Scientific)/ Dawn 8 (Wyatt)/ Optilab (Wyatt)
- Interférométrie (BLI) : Octet RED96 et Blitz (ForteBio, Molecular Devices)
- Gel filtration analytique/Detection par fluorescence (FSEC) : HPLC Dionex/détecteur de fluorescence (Thermo Scientific)
- Nano differential scanning fluorimetry (nanoDSF) : Tycho (NanoTemper Technologies GmbH)
- Diffusion dynamique de la lumière (DLS) : Zetasizer (Malvern Panalytical)
- Dichroïsme circulaire (CD) : J-810 (Jasco)
Publications
- Fuchsbauer O, et al. (2019) Cas9 Allosteric Inhibition by the Anti-CRISPR Protein AcrIIA6. Mol Cell. 2765(19):30697-5.
- Bauer L, et al. (2019) Fluoxetine Inhibits Enterovirus Replication by Targeting the Viral 2C Protein in a Stereospecific Manner. ACS Infect Dis. 5 (9):1609-1623
- Tsika AC, et al. (2019) Deciphering the Nucleotide and RNA Binding Selectivity of the Mayaro Virus Macro Domain. J Mol Biol. 431(12):2283-2297
- Nguyen PC, et al. (2018) Biochemical and Structural Characterization of TesA, a Major Thioesterase Required for Outer-Envelope Lipid Biosynthesis in M. tuberculosis. J Mol Biol 430 : 5120-5136
- Nguyen VS, et al. (2018) Towards a complete structural deciphering of Type VI secretion system. Curr Opin Struct Biol 49 77-84
- Leone P, et al. (2018) Type IX secretion system PorM and gliding machinery GldM form arches spanning the periplasmic space. Nat Commun 9 429
- Nguyen VS, et al. (2017) Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex. Nat Microbiol 2 17103
- Ravachol J, et al. (2016) Mechanisms involved in xyloglucan catabolism by the cellulosome-
- Foos N, et al. (2015) A flexible extension of the Drosophila ultrabithorax homeodomain defines a novel Hox/PBC interaction mode. Structure 23(2):270-9
- De la Cruz MA, et al. (2013) A toxin-antitoxin module of Salmonella promotes virulence in mice. PLoS Pathog. 12:e1003827
- Mate MJ, et al. (2012) Crystal structure of the DNA-bound VapBC2 antitoxin/toxin pair from Rickettsia felis. Nucleic Acids Res 40 3245-58
- Douzi B, et al. (2011) Deciphering the Xcp Pseudomonas aeruginosa type II secretion machinery through multiple interactions with substrates. J Biol Chem. 40792-801
- Felisberto-Rodrigues C, et al. (2011) Towards a structural comprehension of bacterial type VI secretion systems : characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathog. 7 e1002386
- Habchi J, et al. (2011) Characterization of the interactions between the nucleoprotein and the phosphoprotein of Henipavirus. J Biol Chem. 13583-602
- Sciara G et al (2010) Structure of lactococcal phage p2 baseplate and its mechanism of activation. Proc Natl Acad Sci USA. 107 6852-7
- Veesler D, et al. (2009) Production and biophysical characterization of the CorA transporter from Methanosarcina mazei. Anal Biochem. 388 115-21.
