Le service « techniques biophysiques » offre un ensemble d’équipements de pointe pour la caractérisation de complexes moléculaires et le criblage ou l’analyse d’interactions moléculaires, en solution ou sur support solide. Ces outils sont particulièrement adaptés à l’étude des complexes de type :
- protéine/protéine
- protéine/acide nucléique
- protéine/petite molécule
- protéine/détergent
Accessibilité
Académiques et industriels
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Comment faire une demande ?
Compléter le formulaire à partir de http://frisbi.eu/submit-a-proposal/ en cliquant “Marseille” à partir de l’item “Biophysical facility” Contact AFMB : Maria Mate
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Equipement spécifique
- Titration Calorimétrique Isotherme (ITC) : MicroCal iTC200 (Malvern Panalytical)
- Thermophorèse à micro échelle (MST) : Monolith NT.115 (NanoTemper Technologies GmbH)
- Gel filtration analytique/diffusion statique de la lumière multi angle/ réfractométrie (SEC-MALS) :
HPLC Ultimate 3000 (Thermo Scientific)/ Dawn 8 (Wyatt)/ Optilab (Wyatt) - Interférométrie (BLI) : Octet RED96 et Blitz (ForteBio, Molecular Devices)
- Gel filtration analytique/Detection par fluorescence (FSEC) : HPLC Dionex/détecteur de fluorescence (Thermo Scientific)
- Nano differential scanning fluorimetry (nanoDSF) : Tycho (NanoTemper Technologies GmbH)
- Diffusion dynamique de la lumière (DLS) : Zetasizer (Malvern Panalytical)
- Dichroïsme circulaire (CD) : J-810 (Jasco)
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Coût
A définir en fonction du projet.
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