Latest Publications
- An evolutionary perspective on the first disulfide bond in members of the cholinesterase-carboxylesterase (COesterase) family: Possible outcomes for cholinesterase expression in prokaryotes. (2019) Chatonnet A, Brazzolotto X, Hotelier T, Lenfant N, Marchot P, Bourne Y. Chem Biol Interact 308 179-184 PMID:31100280
- Substrate binding mode and catalytic mechanism of human heparan sulfate d-glucuronyl C5 epimerase. (2019) Debarnot C, Monneau YR, Roig-Zamboni V, Delauzun V, Le Narvor C, Richard E, Henault J, Goulet A, Fadel F, Vives RR, Priem B, Bonnaffe D, Lortat-Jacob H, Bourne Y. Proc Natl Acad Sci U S A in press PMID:30872481
- Overview of the Structure-Function Relationships of Mannose-Specific Lectins from Plants, Algae and Fungi. (2019) Barre A, Bourne Y, Van Damme EJM, Rouge P. Int J Mol Sci 20 PMID:30634645
- Genetic and enzymatic characterization of 3-O-sulfotransferase SNPs associated with Plasmodium falciparum parasitaemia. (2018) Nguyen NT, Vives RR, Torres M, Delauzun V, Saesen E, Roig-Zamboni V, Lortat-Jacob H, Rihet P, Bourne Y. Glycobiology 28 534-541 PMID:29718295
- Lytic xylan oxidases from wood-decay fungi unlock biomass degradation. (2018) Couturier M, Ladeveze S, Sulzenbacher G, Ciano L, Fanuel M, Moreau C, Villares A, Cathala B, Chaspoul F, Frandsen KE, Labourel A, Herpoel-Gimbert I, Grisel S, Haon M, Lenfant N, Rogniaux H, Ropartz D, Davies GJ, Rosso MN, Walton PH, Henrissat B, Berrin JG. Nat Chem Biol 14 306-310 PMID:29377002
- Hot spots for protein partnership at the surface of cholinesterases and related alpha/beta hydrolase fold proteins or domains - a structural perspective. (2017) Bourne Y, Marchot P. Molecules 23 35 PMID:29295471
Our research projects address the molecular architectures and functional mechanisms of carbohydrate-active enzymes (CAZymes), sensor domains and lectins, and of enzymes, receptors and cell adhesion molecules with a neurobiological interest.
The CAZymes govern the hydrolysis, conversion or recognition of sugar moieties from glycoconjuguates widely found in animals, fungi, plants and bacteria. These conjugates are involved in key biological processes such as development, fertilization, quality control of protein folding, host-pathogen recognition, immune response, and they can be linked to diseases such as neurodegenerative metabolic diseases. In collaboration with the “Glycogenomics” team we pay particular attention to those glycoside hydrolases that are of interest for biomedical and biotechnological applications.
We also address the function, recognition properties and structure of receptors, enzymes and adhesion molecules involved in central and peripheral neurotransmission, with particular interest for their structure-function relationships, specificity of partner and ligand recognition, conformational dynamics and functional regulation. In particular we have accumulated expertise in the study of the enzyme acetylcholinesterase and of the extracellular ligand-binding domain of the nicotinic receptor to acetylcholine whose malfunctioning has been correlated with neurodegenerative diseases, and to cell adhesion molecules structurally related to the cholinesterases or to other families of proteins and associated with developmental neuronal deficiencies.
Our methodological approaches involve recombinant protein expression in prokaryotic and eukaryotic systems and complementary biochemical, biophysical, functional and structural (X-ray crystallography and electron microscopy) characterization means.
The team
Yves BOURNE (Senior researcher)
Pascale MARCHOT (Senior researcher)
Violla BASSIM (Ph.D. student)
Jean-Baptiste BOITEL (Engineer)
Sarah COULIBALY MARTINEAU (Master student)
Nicola CURCI (Ph.D. student)
Claire DEBARNOT (Engineer)
Antonin GARDAIS (Master student)
Semeli PLATSAKI (Postdoctoral fellow)
Gerlind SULZENBACHER (Research engineer)
Florence VINCENT (Researcher)
Véronique ZAMBONI (Assistant engineer)
Former team members and trainees
Caroline Peneff, PhD student, 1999-2001
Jordan Meynard, Master-2 BBSG student, 2003
David Veesler, Master-1 BBSG student, 2004
Dorothée Bauguil, 2nd year ESIL (now Polytech Marseille) student, 2004
Abraham Saliba, Master-2 BBSG student, 2004
Julien Offant, PhD student, 2003-2005
Isabelle Dezoize, 2nd year ESIL (now Polytech Marseille) student, 2006
Alexandra Lipski, Master 2 BBSG and PhD student, 2006-2008
David Dal Molin, 2nd year ESIL (now Polytech Marseille) student, 2008
Mahfoud Balki, Master-2 BBSG student, 2009
Emma Bossion, 2nd yrs BTS student Lycée La Forbine, 2009
Marie-Cécile Pélissier, Master-2 BBSG and PhD student, 2006-2009 (ATER 2009)
Goetz Parsiegla, Maître de Conférences, 2001-2010
Allan Lamontanara, 2nd year ESIL (now Polytech Marseille) student, 2010
Daniel Gonzales, Master-1 BBSG student, 2010
Nahla Dhen, Master-2 BBSG student, 2011
Julie Prats, 2nd year ESIL (now Polytech Marseille) student, 2012
Guillaume Ponchel, Master-2 BBSG and PhD student, 2008-2012
Isabelle Mosnier, engineer assistant, 2011-2012
Claire Zimberger, BTS student Lycée Marie Curie, 1rst and 2nd years, 2011 and 2012
Thibaud Freyd, Master-2 BBSG student, 2012
Camille Ribeyre, Master-1 BBSG student, 2013
Nicolas Lenfant, postdoc/visitor INRA Montpellier, 2011-2013
Audrey Blanchard, study engineer, 2011-2014
Supanee Potisopon, postdoc/research engineer, 2014
Florian Mourey, 2nd year AgroParisTech student, 2014
Stanley Germany, Master-2 BBSG student, 2014
Romain La Rocca, L3 student, 2015
Aurélien Assice-Vivoni, study engineer, 2015
Julie Baptiste, study engineer, 2015-2016
Camille Garcia, L3 student, 2016
Sandy Bezenger, Master-2 BBSG student, 2016
Farida Nezlioui, Master-2 BBSG student, 2016
Ahmad Ali-Ahmad, Master-2 BBSG and PhD student, 2011-2016
Maria Mate, research engineer CNRS, 2013-2017
Marie-Julie Dejardin, study engineer, 2016-2017
Adeline Goulet, research engineer, then postdoc FRM, and then CNRS researcher, 2013-2017
Yoann Crétinon, Master-1 BBSG student, 2017
Marina Gomes, Master-1 and Master-2 BBSG student, 2016-2017
Quoc-Phong Nguyen, Master-2 student, 2017
Melvin Boumedour, 1rst and 2nd yrs BTS student Lycée La Forbine, 2017
Lamia Mebarki, PhD student, then study/research engineer, 2014-2017
Amani Ghanem, Master-2 BBSG student, 2018
Viet-Anh Hoang, Master-2 student, Vietnam, 2018
Firas Fadel, post-doctoral fellow, 2014-2018
Elodie Caillol, L3 student, Univ Grenoble Alpes, 2019
Sarah Coulibaly Martineau, Master-2 BSG student, 2019
