CNRS - AIX MARSEILLE UNIV: UMR7257

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Viral Replicases: Structure, function and drug-design

Head Bruno CANARD

Latest Publications

  1. Filovirus proteins for antiviral drug discovery: Structure/function bases of the replication cycle. (2017) Martin B, Canard B, Decroly E. Antiviral Res in press PMID:28192094
  2. Binding of the methyl donor SAM to MERS-CoV 2' -O-methyltransferase nsp16 promotes the recruitment of the allosteric activator nsp10. (2017) Aouadi W, Blanjoie A, Vasseur JJ, Debart F, Canard B, Decroly E. J Virol in press PMID:28031370
  3. The Zika virus methyltransferase: structure and functions for drug design perspectives. (2017) Coutard B, Barral K, Lichiere J, Selisko B, Martin B, Aouadi W, Ortiz Lombardia M, Debart F, Vasseur JJ, Guillemot JC, Canard B, Decroly E. J Virol in press PMID:28031359
  4. Coxsackievirus B3 protease 3C: expression, purification, crystallization and preliminary structural insights. (2016) Fili S., Valmas A., Christopoulou M., Spiliopoulou M., Nikolopoulos N., Lichiere J., logotheti S., Karavassili F., Rosmaraki E., Fitch A., Wright A., Beckers D., Degen T., Nenert G., Hilgenfeld R., Papageorgiou N., Canard B., Coutard B., Margiolaki I.. Acta Crystallogr F Struct Biol Commun. 72(Pt 12) 877-884 PMID:27917835
  5. Involvement of an Arginine Triplet in M1 Matrix Protein Interaction with Membranes and in M1 Recruitment into Virus-Like Particles of the Influenza A(H1N1)pdm09 Virus. (2016) Kerviel A, Dash S, Moncorge O, Panthu B, Prchal J, Decimo D, Ohlmann T, Lina B, Favard C, Decroly E, Ottmann M, Roingeard P, Muriaux D. PLoS One 11 e0165421 PMID:27814373
  6. Discovery of novel dengue virus NS5 methyltransferase non-nucleoside inhibitors by fragment-based drug design. (2016) Benmansour F, Trist I, Coutard B, Decroly E, Querat G, Brancale A, Barral K. Eur J Med Chem 125 865-880 PMID:27750202
...All publications

Our team seeks to unravel the molecular mechanisms of emerging viruses by characterizing the structures and the enzymatic activities of proteins forming the viral replication and transcription complex. These studies are a prerequisite for the development of specific inhibitors of these enzymes and should allow the development of new antiviral strategies.

RNA viruses are often associated with the emergence of infectious diseases. Among the best known, epidemics related to Ebola, SARS coronavirus and MERS, chikungunya or dengue virus illustrate the worldwide public health concern related to viral infections. The team "Viral replicase: structure, mechanism and drug-design" characterizes enzymes and proteines from emerging viruses involved in the replication of their genome and transcription of their messenger RNAs. These enzymes are associated in a replication/transcription complex (RTC). Our studies focus not only on viral polymerases that are at the heart viral replication, but also on the enzymes involved in RNA modifications such as capping and proofreading and proteins involved in the regulation of replication. Beyond the study of the RTC, we are also interested in the interplay of these enzymes with the innate immunity defence mechanism of the cell. The viral replication proteins are privileged antiviral targets and understanding of their structure and function is essential for the design of antiviral molecules with a high potential. To support these research projects, the lab benefits from the support of an inhibitor-screening platform (PCML) and a platform dedicated to the expression of recombinant viral proteins.


Wahiba AOUADI
UNK
Sarah ATTOUMANI
Amal BAKLOUTI
Bruno CANARD
Bruno COUTARD
Etienne DECROLY
UNK
Awa DIOP
UNK
Sylvie DOUBLIE
Cécilia EYDOUX
UNK
Veronique FATTORINI
UNK
Ana-Sofia FERREIRA-RAMOS
François FERRON
Jean-Claude GUILLEMOT
Isabelle IMBERT
Nhung LE
Philippe LIEUTAUD
Baptiste MARTIN
Ana-Theresa MORAIS
UNK
Thi-Hong-Van NGUYEN
Nicolas PAPAGEORGIOU
Nadia RABAH
Barbara SELISKO
UNK
Coralie VALLE
Elsie YEKWA

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